Abstract
The crosslinking of fibrin γ-polypeptide chains under the influence of the plasma fibrin-stabilizing factor (FXIIIa), which causes their conversion to γ-γ dimers, is the major enzyme reaction of covalent fibrin stabilization. We studied the self-assembly of soluble cross-linked fibrin oligomers. The results of analytical ultracentrifugation as well as elastic and dynamic light scattering showed that the double-stranded fibrin oligomers formed under the influence of moderate concentrations of urea are cross-linked only due to formation of γ-γ dimers, which can dissociate into single-stranded structure when the concentration of urea increases. This fact proves that γ-γ dimers are formed in the end-to-end manner.
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Richardson, V.R., Cordell, P., Standeven, K.F., and Carter, A.M., Clin. Sci., 2013, vol. 124, pp. 123–137.
Weisel, J.W., Francis, C.W., Nagaswani, C., and Marder, V.J., J. Biol. Chem., 1993, vol. 268, pp. 26618–26624.
Veklich, Y., Ang, E.K., Lorand, L., and Weisel, J.W., Proc. Nat. Acad. Sci. U.S.A., 1998, vol. 95, pp. 1438–1442.
Mosesson, M.W., J. Thromb. Haemost., 2004, vol. 2, pp. 388–393.
Siebenlist, K.R., Meh, D.A., and Mosesson, M.W., Biochemistry, 2000, vol. 39, pp. 14171–14175.
Rosenfeld, M.A., Leonova, V.B., Vasileva, M.V., and Biryukova, M.I., Biochemistry (Moscow), 2011, vol. 76, no. 10, pp. 1155–1163.
Rosenfeld, M.A., Bychkova, A.V., Shchegolikhin, A.N., Leonova, V.B., Biryukova, M.I., and Kostanova, E.A., Biochim. Biophys. Acta., 2013, vol. 1834, pp. 2470–2479.
Rosenfeld, M.A., Gershkovich, K.B., Kuznetsov, D.V., Meshkov, B.B., and Gontar’, I.D., Mol. Biol. (Moscow), 1986, vol. 20, pp. 1098–1110.
Rosenfeld, M.A., Gershkovich, K.B., and Kuznetsov, D.V., Mol. Biol. (Moscow), 1988, vol. 22, pp. 86–93.
Rosenfeld, M.A., Shchegolikhin, A.N., Bychkova, A.V., Leonova, V.B., Biryukova, M.I., and Kostanova, E.A., Free Radic. Biol. Med., 2014, vol. 77, pp. 106–120.
Ñasassa, E.F., J. Chem. Phys., 1955, vol. 23, pp. 596–597.
Bernocco, S., Ferri, F., Profumo, A., Cuniberti, C., and Rocco, M., Biophys. J., 2000, vol. 79, pp. 561–583.
Mosesson, M.W., Siebenlist, K.R., Hainfeld, F., and Wall, J.S., J. Struct. Biol., 1995, vol. 115, pp. 88–101.
Weisel, J.W., J. Thromb. Haemost., 2004, vol. 2, pp. 394–399.
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Original Russian Text © M.A. Rosenfeld, V.B. Leonova, A.V. Bychkova, E.A. Kostanova, M.I. Biryukova, 2015, published in Doklady Akademii Nauk, 2015, Vol. 464, No. 2, pp. 234–237.
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Rosenfeld, M.A., Leonova, V.B., Bychkova, A.V. et al. Longitudinal orientation of cross-linked polypeptide γ chains in fibrin fibrils. Dokl Biochem Biophys 464, 286–289 (2015). https://doi.org/10.1134/S1607672915050051
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DOI: https://doi.org/10.1134/S1607672915050051