Abstract
A new method of evaluation of beta-receptor’s activity on the surface of human T-lymphocytes has been proposed based on the radioligand method. Optimal conditions for evaluation of specific binding to β2-adrenoceptors of 0.5 fmol ligand per 1 million cells using [125I] -cyanopindolol were found. The possibility of using β2-adrenoceptor’s activity assessment in clinical settings was demonstrated on human T-lymphocytes.
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Abbreviations
- PBS:
-
5 mM potassium phosphate buffer with 0.14 M NaCl
- pH 7.0; BSA:
-
bovine serum albumin
- PKA:
-
protein kinase A
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Original Russian Text © O.Y. Agapova, Y.S. Skoblov, K.A. Zykov, A.V. Rvacheva, V.B. Beilina, V.P. Masenko, I.E. Chazova, 2015, published in Bioorganicheskaya Khimiya, 2015, Vol. 41, No. 5, pp. 592–598.
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Agapova, O.Y., Skoblov, Y.S., Zykov, K.A. et al. Radioligand method of assessment of human T-lymphocytes’ β-adrenoceptors activity. Russ J Bioorg Chem 41, 529–535 (2015). https://doi.org/10.1134/S1068162015050027
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DOI: https://doi.org/10.1134/S1068162015050027