Abstract
The seasonal dynamics of diarrhetic shellfish poisoning (DSP) toxins in the digestive gland of the mussel Crenomytilus grayanus (Dunker, 1853) from Peter the Great Bay in the Sea of Japan have been studied. Toxins in the form of 4-bromomethyl-7-methoxycoumarin derivatives have been analyzed using high-performance liquid chromatography (HPLC) with fluorometric detection. This method allows the determination of the quantitative and qualitative composition of individual toxins. All the C. grayanus samples contained DSP toxins, but their concentration varied substantially during the year. The highest concentration was recorded from the mussels collected in May; the lowest concentration was recorded in February. The toxins were represented predominantly by dinophysistoxin-2; in July-September and December, okadaic acid was detected. An assumption has been made about the relationship between the potential producers of DSP toxins in the waters of the Peter the Great Bay and the dynamics of these toxins in tissues of C. grayanus. The HPLC method, as an alternative to the enzyme-linked immunosorbent assay, was used to assess the quantitative and qualitative composition of DSP toxins in seafood for the first time in the Russian Federation. This method can be recommended for the routine monitoring of toxins conducted by the controlling organizations of the Russian Federal Service for Veterinary and Phytosanitary Surveillance.
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Yedinye sanitarno-epidemiologicheskiye i gigienicheskiye trebovaniya k produktsii (tovaram), podlezhashchei sanitarno- epidemiologicheskomu nadzoru (kontrolyu) (s izmeneniyami na 10 noyabrya 2015 goda): utv. resheniem Komissii Tamozhennogo Soyuza ot 28.05.2010 g. N 299. (Uniform Sanitary, Epidemiological, and Hygienic Requirements to the Product (Goods) Subject to Sanitary and Epidemiologic Supervision (Control) (with amendments on November 10, 2015), Approved by Decision of the Customs Union Commission, May 28, 2010, no. 299).
Konovalova, G.V., Dinoflagellyaty (Dinophyta) dal’nevostochnykh morei Rossii i sopredel’nykh akvatorii Tikhogo okeana (Dinoflagellates (Dinophyta) in the Far Eastern Seas of Russia and Adjacent Waters of the Pacific Ocean), Vladivostok: Dal’nauka, 1998.
Kokhanova, Yu.A. and Khotimchenko, S.A., Biosafety: phytotoxins (part 3), Vopr. Pitaniya, 2006, no. 4, pp. 16–19.
Orlova, T.Yu., Monitoring of toxic microalgae as a basis for the biological safety of coastal waters and seafood, in Materialy Tselevoi kompleksnoi programmy orientirovannykh fundamental’nykh nauchnykh issledovanii Dal’nevostochnogo otdeleniya Rossiiskoi Akademii Nauk na 2007–2012 gg.: “Biologicheskaya bezopasnost’ dal’nevostochnykh morei Rossiiskoi Federatsii” (Materials of the Target Complex Program Oriented to the Fundamental Researches at the Far Eastern Branch, Russian Academy of Sciences for 2007–2012: “Biological Safety of the Far Eastern Seas of the Russian Federation”), Adrianov, A.V., Ed., Vladivostok: Dal’nauka, 2014, pp. 309–324.
Selina, M.S., Distribution of potentially toxic dinoflagellates Dinophysis (Dinophyta) in Vostok Bay, Sea of Japan, Russ. J. Mar. Biol., 1993, vol. 19, nos. 5–6, pp. 299–302.
Sychev, K.S., Prakticheskii kurs zhidkostnoi khromatografii (Practical Course on Liquid Chromatography), Moscow: KOKORO, 2013.
Tekhnicheskii Reglament Tamozhennogo Soyuza TRTS 021/2011 “O bezopasnosti pishchevoi produktsii”: utv. resheniem Komissii Tamozhennogo Soyuza ot 9.12.2011 g. N 880 (Technical Regulations of the Customs Union TRTS 021/2011 “On Safety of Food Products”, Approved by Decision of the Customs Union Commission, December 9, 2011, no. 880).
Metodicheskiye rekomendatsii MR01.016-07: “Ekspress-opredeleniye okadaikovoi kisloty v mollyuskakh s pomoshch’yu test-sistemy DSP-Check proizvodstva firmy Parapharm Laboratories Co., Ltd, Yaponiya” (Methodical Recommendations MR01.016-07: “Rapid Detection of Okadaic Acid in Shellfish Using the Test System DSP-Check Manufactured by Parapharm Laboratories, Japan”), Moscow: Rospotrebnadzor, 2007.
Blanco, J., Marino, C., Martin, H., et al., Anatomical distribution of diarrhetic shellfish poisoning (DSP) toxins in the mussel Mytilus galloprovincialis, Toxicon, 2007, vol. 50, no. 8, pp. 1011–1018.
Carmody, P., James, J.K., and Kelly, S.S., Dinophysistoxin- 2: the predominant diarrhetic shellfish toxin in Ireland, Toxicon, 1996, vol. 34, no. 3, pp. 351–359.
Garcia, C., Truan, D., Lagos, M., et al., Metabolic transformation of dinophysistoxin-3 into dinophysistoxin- 1 causes human intoxication by consumption of O-acyl-derivatives dinophysistoxins contaminated shellfish, J. Toxicol. Sci., 2005, vol. 30, no. 4, pp. 287–296.
Hammert, C., Lucas, B., and Kirschbaum, J., HPLC/MS determination of diarrhetic shellfish poisoning (DSP) toxins as 4-bromomethyl-7-methoxycoumarin derivatives, in Harmful Marine Algal Blooms, Paris: Lavoisier, 1995, pp. 297–302.
Hoppenrath, M., Chomerat, N., Horiguchi, T., et al., Taxonomy and phylogeny of the benthic Prorocentrum species (Dinophyceae)—a proposal and review, Harmful Algae, 2013, vol. 27, pp. 1–28.
Imbs, A.B., Vologodskaya, A.V., Nevshupova, N.V., et al., Response of prostaglandin content in the red alga Gracilaria verrucosa to season and solar irradiance, Phytochemistry, 2001, vol. 58, no. 7, pp. 1067–1072.
Kameneva, P.A., Efimova, K.V., Rybin, V.G., et al., Detection of dinophysistoxin-1 in clonal culture of marine dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan, Toxins, 2015, vol. 7, no. 10, pp. 3947–3959.
Kameneva, P.A., Imbs, A.B., and Orlova, T.Yu., Distribution of DTX-3 in edible and non-edible parts of Crenomytilus grayanus from the Sea of Japan, Toxicon, 2015, vol. 93, pp. 1–3.
Lee, J.S., Igarashi, T., Fraga, S., et al., Determination of diarrhetic shellfish toxins in various dinoflagellate species, J. Appl. Phycol., 1989, vol. 1, no. 2, pp. 147–152.
Lee, J.S., Yanagi T., Kenma, R., et al., Fluorometric determination of diarrhetic shellfish poisoning by high performance liquid chromatography, Agric. Biol. Chem., 1987, vol. 51, no. 3, pp. 877–881.
Leftley, J. and Hannah, F., Phycotoxins in seafood, in Bioactive Compounds in Foods, London: Wiley, 2008, pp. 52–108.
Luckas, B., Hummert, C., Thielert, G., et al., Improvement of HPLC/fluorescence and HPLC/MS procedures for the determination of DSP and PSP toxins in contaminated shellfish, in EUR Report No. 15339, Luxembourg: Eur. Comm., 1994.
Marr, J.C., McDowell, L.M., and Quilliam, M.A., Investigation of derivatization reagents for the analysis of diarrhetic shellfish poisoning toxins by liquid chromatography with fluorescence detection, Nat. Toxins, 1994, vol. 2, no. 5, pp. 302–311.
McNabb, P., Chemistry, metabolism and chemical analysis of okadaic acid group toxins, in Seafood and Freshwater Toxins: Pharmacology, Physiology and Detection, Boca Raton: CRC, 2008, pp. 209–228.
Morton, S., Vershinin, A., Laurinda, L.S., et al., Seasonality of Dinophysis spp. and Prorocentrum lima in Black Sea phytoplankton and associated shellfish toxicity, Harmful Algae, 2009, vol. 8, no. 5, pp. 629–636.
Orlova, T.Yu., Toxic microalgae in plankton, benthic and epiphytic assemblages from the north-western Sea of Japan, Proc. 9th IST Asia Pacific Meeting “Animal, Plant and Microbial Toxins,” Vladivostok, Russia, September 4–8, 2011, Vladivostok: Dal’nauka, 2011, p. 40.
Orlova, T.Yu., Kameneva, P.A., Stonik, I.V., et al., Diarrhetic shellfish toxins in Primorsky krai, Russia, J. Shellfish Res., 2015, vol. 34, no. 3, pp. 1151–1160.
Reguera, B., Velo-Suarez, L., Raine, R., et al., Harmful Dinophysis species: a review, Harmful Algae, 2012, vol. 14, pp. 87–106.
Stabell, O.B., Hormazabal, V., Steffenak, I., et al., Diarrhetic shellfish toxins: improvement of sample clean-up for HPLC determination, Toxicon, 1991, vol. 29, no. 1, pp. 21–29.
Suzuki, T., Ota, H., and Yamasaki, M., Direct evidence of transformation of dinophysistoxin-1 to 7-O-acyldinophysistoxin- 1 (dinophysistoxin-3) in the scallop Patinopecten yessoensis, Toxicon, 1999, vol. 37, no. 1, pp. 187–198.
Takai, A., Murata, M., Torigoe, K., et al., Inhibitory effect of okadaic acid derivatives on protein phosphatases, Biochem. J., 1992, vol. 284, no. 2, pp. 539–544.
Valdiglesias, V., Prego-Faraldo, M.V., Pasaro, E., et al., Okadaic acid: more than a diarrheic toxin, Mar. Drugs, 2013, vol. 11, no. 11, pp. 4328–4349.
Vale, P. and Sampayo, M.A., Esterification of DSP toxins by Portuguese bivalves from the northwest coast determined by LC-MS—a widespread phenomenon, Toxicon, 2002, vol. 40, no. 1, pp. 33–42.
Vershinin, A., Moruchkov, A., Morton, S., et al., Phytoplankton composition of the Kandalaksha Gulf, Russian White Sea: Dinophysis and lipophilic toxins in the blue mussel (Mytilus edulis), Harmful Algae, 2006, vol. 5, no. 5, pp. 558–564.
Yanagi, T., Murata, M., Torigoe, K., et al., Biological activities of semisynthetic analogs of DTX-3 and major diarrhetic shellfish toxins, Agric. Biol. Chem., 1989, vol. 53, no. 2, pp. 525–529.
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Original Russian Text © P.A. Kameneva, T.Yu. Orlova, A.B. Imbs, 2016, published in Biologiya Morya.
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Kameneva, P.A., Orlova, T.Y. & Imbs, A.B. The seasonal dynamics of diarrhetic shellfish poisoning toxins in the digestive gland of Crenomytilus grayanus (Dunker, 1853) analyzed by high-performance liquid chromatography with fluorometric detection. Russ J Mar Biol 42, 258–265 (2016). https://doi.org/10.1134/S1063074016030056
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DOI: https://doi.org/10.1134/S1063074016030056