Abstract
By using morphometric and molecular-genetic methods, the population of honeybees of apiaries located in the mountain-forest zone of the Crimean Peninsula under conditions of prolonged isolation from other bee farms of the peninsula was studied. The hypothesis that the bees of this apiary may show signs of lost Crimean bees was analyzed. The complex of morphological traits makes the bees of the examined apiaries closer to Italian, Krajina, and Ukrainian steppe bees and, to a lesser extent, to Carpathian bees. The results of molecular genetic analysis of the initial part of the mtDNA COI gene from GenBank for the races under consideration revealed the evolutionary proximity of the bees studied to representatives of Italian bees. The bees of the isolated apiary are united into a single haplotype. The sequences of the mtDNA COI locus were obtained from Carpathian bees from Moscow oblast and Tajikistan. In terms of differences between the Italian bee haplotype and haplotypes of bees from other regions related to Carpathian bees, it is suggested that the haplotypes of the apiary studied are more similar to Carpathian bees. The investigations do not reject the hypothesis that bees of the isolated apiary may have traits typical of Crimean bees.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Alpatov, V.V., Variability of bee proboscis in biometric research and practical issues related to the problem of honeybee breeding, IV Int. Congr. Entomol., 1929, vol. 2, pp. 1010–1019.
Alpatov, V.V., To the knowledge of the honey bee variability. VI. Bees of Ukraine and Crimea and their place among other forms of Apis mellifera L., Zool. Zh., 1938, vol. 17, no. 3, pp. 473–480.
Alpatov, V.V., Porody medonosnoi pchely (Honeybee Breeds), Moscow: Izd. MOIP, 1948.
Bandelt, H.J., Foster, P., and Röhl, A., Median-joining networks for inferring intraspecific phylogenies, Mol. Biol. Evol., 1999, vol. 16, no. 1, pp. 37–48.
Bilash, G.D. and Kravtsov, N.I., Population systematics of the honeybee, Pchelovodstvo, 1985, no. 10, pp. 11–13.
Brandorf, A.Z., Ivoilova, M.M., Il’yasov, R.A., Poskryakov, A.V., and Nikolenko, A.G., Populationgenetic differentiation of honeybees of Kirov oblast, Pchelovodstvo, 2012, no. 7, pp. 14–16.
Crozier, R.H. and Crozier, Y.C., The mitochondrial genome of the honeybee Apis mellifera: complete sequence and genome organization, Genetics, 1993, vol. 133, no. 1, pp. 97–117.
Engel, M.S., The taxonomy of recent and fossil honey bees (Hymenoptera: Apidae, Apis), J. Hym. Res., 1999, vol. 8, pp. 165–196.
Excoffier, L. and Lischer, H.E.L., Arlequin suite ver. 3.5: a new series of programs to perform population genetics analyses under Linux and Windows, Mol. Ecol. Res., 2011, vol. 10, pp. 564–567.
Gaidar, V.A., Kerek, S.S., Papp, V.V., and Kerek, P.M., Morphoecological standard of Carpathian bees, Pchelovodstvo, 2004, no. 6, p. 14.
Gubin, V.A., Carpathian population of Carnica bee, Pchelovodstvo, 1972, no. 5, pp. 28–31.
Il’yasov, R.A., Polymorphism of Apis mellifera L. in the Urals, Extended Abstract of Cand. Sci. (Biol.) Dissertation, Ufa: Inst. Biokhim. Genet. UfNTs RAN, 2006.
Itenov, K. and Pedersen, B.V., Nucleotide sequence differences in the honey-bee subspecies Apis mellifera mellifera and Apis mellifera ligustica, Tidsskrift Planteavl. Bind., 1991, vol. 95, pp. 101–103.
Konusova, O.L., Pogorelov, Yu.L., Ostroverkhova, N.V., Rasseikina, S.A., Nechipurenko, A.O., Vorotov, A.A., Klimova, E.A., and Prokop’ev, A.S., Biological and economic evaluation of families of the honeybee (Apis mellifera L.) in some parts of Tomsk oblast, Vestn. Tomsk. Univ.: Biol., 2010, no. 1 (9), pp. 29–41.
Kozhevnikov, G.A., Materials of the natural history of the bee (Apis mellifera L.). Vol. 1, Izv. Obshch. Lyubit. Estestvoznan. Antropol. Etnogr., vol. XCIX, Tr. Zool. Otd., 1900, no. XIV.
Krivtsov, N.I., Lebedev, V.I., and Tunikov, G.M., Pchelovodstvo (Beekeeping), Moscow: Kolos, 1999.
Krivtsov, N.I. and Sokol’skii, S.S., Porody pchel i ikh selektsiya (Breeds of Bees and Their Breeding), Rybnoe: NIIP, 2010.
Lebedev, V.I. and Bilash, N.G., Biologiya medonosnoi pchely (Honeybee Biology), Moscow: Agropromizdat, 1991.
Morev, I.A., Moreva, L.Ya., and Kozub, M.A., Ecological plasticity of bee populations of the south of Russia, Vestn. Tambov. Univ., 2013, vol. 18, no. 3, pp. 890–895.
Petrov, P., Population structure of the honeybee Apis mellifera rodopica in Bulgaria, Pchelarstvo, 1995, vols. 1–2, pp. 101–105.
Ruttner, F., Die Zuchtauslese bei der Biene: Anleitung zur Kör- und Belegstellenpraxis, München: Ehrenwirth, 1967.
Ruttner, F., Biogeography and Taxonomy of Honeybees, Berlin: Springer Verlag, 1988.
Ruttner, F., Naturgeschichte der Honigbienen, Munich, Germany: Ehrenwirth Verlag; Grenoble; Bukarest: Apimondia, 1992.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S., Mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Mol. Biol. Evol., 2011, vol. 28, pp. 2731–2739.
Tormosina, T.T., Comparative study of Carpathian bees of different origin in the North Caucasus, Extended Abstract of Cand. Sci. (Agric.) Dissertation, Moscow: MTSKhA, 1985.
Triselyova, T.A., Akent’eva, N.A., and Safonkin, A.F., Phylogenetic relations between frit fly groups from the genus Meromyza based on genetic and morphological analysis, Biol. Bull. (Moscow), 2014, vol. 41, no. 3, pp. 203–207.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © T.O. Bykova, T.A. Triseleva, A.V. Ivashov, A.F. Safonkin, 2016, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2016, No. 6, pp. 625–630.
Rights and permissions
About this article
Cite this article
Bykova, T.O., Triseleva, T.A., Ivashov, A.V. et al. Morphogenetic diversity of the honeybee Apis mellifera L. from the mountain-forest zone of Crimea. Biol Bull Russ Acad Sci 43, 541–546 (2016). https://doi.org/10.1134/S1062359016060054
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1062359016060054