Abstract
A procedure is proposed for determining Meldonium in human urine, including sample preparation to analysis and analyte determination by HPLC with tandem mass spectrometric detection. For sample preparation, the procedure of “dilute-and-shoot” was used. The lower limit of the analytical range is 10 ng/mL; the limit of detection is 7.5 ng/mL; and the linearity range is 10–250 ng/mL. The proposed procedure is tested on real samples obtained from volunteers. A possibility of the direct analysis of urine samples after dilution is demonstrated; the limit of detection is 20 ng/mL. The high sensitivity of the procedure ensures its use for the determination of Meldonium in clinical diagnosis and doping control.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Eremeev, A., Kalvinsh, I., Semenikhina, V., Liepinsh, E., Latvieti, Y., Anderson, P., Astapenok, E., Spruzh, Y., Trapentsiers, P., Podoprigora, G., and Giller, S., US Patent 4481218, 1984.
Dambrova, M., Liepinsh, E., and Kalvinsh, I., Trends Cardiovasc. Med., 2002, vol. 12, p. 275.
Cai, L.-J., Zhang, J., Peng, W.-X., Zhu, R.-H., Yang, J., Cheng, G., and Wang, X.-M., Chromatographia, 2011, vol. 73, p. 659.
Lv, Y.-F., Hu, X., and Bi, K.-S., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2007, vol. 852, p. 35.
Peng, Y., Yang, J., Wang, Z., Wang, J., Liu, Y., Luo, Z., and Wen, A., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2010, vol. 878, p. 551.
Pidpruzhnykov, Y., Sabko, V., Iurchenko, V., and Zupanets, I., Biomed. Chromatogr., 2012, vol. 26, p. 599.
Görgens, C., Guddat, S., Dib, J., Geyer, H., Schänzer, W., and Thevis, M., Drug Test. Anal., 2015, vol. 7, p. 973.
Arduiani, A., Bonomini, M., Savica, V., Amato, A., and Zammit, V., Pharmacol. Ther., 2008, vol. 120, p. 149.
World Anti-Doping Agency: The 2016 Prohibited List. https://wadamainprod.s3.amazonaws.com/resources/files/wada_td2015idcr_minimum _criteria_chromatomass_spectro_conf_en.pdf. Cited January 1, 2016.
Thevis, M., Kuuranne, T., Geyer, H., and Schanzer, W., Drug Test. Anal., 2013, vol. 5, p. 1.
FDA Guidance for Industry: Bioanalytical Method Validation, US Department of Health and Human Services, Food and Drug Administration (FDA), Rockville, USA. http://www.fda.gov/downloads/drugs/guidancecomplianceregulatoryinformation/guidnces/ucm368107.pdf. Cited August 11, 2015.
Identification criteria for qualitative assays incorporating column chromatography and mass spectrometry, WADA Technical Document TD2015IDCR. https://wada-mainprod.s3.amazonaws.com/resources/files/wada_td2015idcr_minimum_criteria_chromatomass_ spectro_conf_en.pdf. Cited March 10, 2016.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.A. Azaryan, A.Z. Temerdashev, E.V. Dmitrieva, 2017, published in Zhurnal Analiticheskoi Khimii, 2017, Vol. 72, No. 10, pp. 885–889.
Rights and permissions
About this article
Cite this article
Azaryan, A.A., Temerdashev, A.Z. & Dmitrieva, E.V. Determination of Meldonium in human urine by HPLC with tandem mass spectrometric detection. J Anal Chem 72, 1057–1060 (2017). https://doi.org/10.1134/S1061934817100033
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1061934817100033