Abstract
This study was aimed at examining two flow injection-spectrophotometric systems (normal and reverse) for the determination of clonazepam (CLO) at the microgram level in pure and pharmaceutical dosage forms. The estimation of CLO has been developed by conjugating a normal (or reverse) flow injection analysis (nFIA or rFIA) and spectrophotometric detection with phloroglucinol as a coupling reagent. Beer’s law was obeyed over a range of 50–400 and 30–400 μg/mL. The limits of detection were 11 and 8 μg/mL and the sampling rates were 51 and 28 samples per hour for nFIA and rFIA respectively. Both systems were successfully applied for the determination of CLO in its commercially available dosage forms. A comparison between the proposed flow systems was also done. These simple and high throughput methods could be utilized for pharmaceutical analysis of CLO.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Clinical Pharmacy and Therapeutics, Epilepsy, P.W.A., Herfindal, E.T., Gourley, D.R., and Hart, L.L., Eds., Maryland: Williams and Wilkins, 1988.
Martindale: The Complete Drug Reference, Pharmaceutical Press, 2009, 36th ed.
The Indian Pharmacopoeia, Ghaziabad: The Indian Pharmacopoeia Commission, 2010, 6th ed., vol.2.
British Pharmacopoeia, London: British Pharmacopoeia Commission, 2007, vol.3.
United State Pharmacopoeia, Rockville: USP Convention, 2007, 30th ed.
Isabelle, F.B., Fabienne, P., and Christian, J., J. Pharm. Biomed. Anal., 2004, vol. 36, p. 865.
Mistsuhrio, N., Kana, F., Tadashi, S., and Yoshihiro, K., Biol. Pharm. Bull., 2004, vol. 27, p. 893.
Chaichi, M.J. and Alijanpour, S.O., Spectrochim. Acta, Part A, 2014, vol. 118, p. 36.
Dai, H., Lin, Y., Wu, X., and Chen, G., Sens. Actuators, B, 2010, vol. 145, no. 1, p. 320.
Habibi, B. and Jahanbakhshi, M., Electrochim. Acta, 2014, vol. 118, p. 10.
Hadi, H., Iraqi J. Pharm. Sci., 2015, vol. 24, no. 1, p. 25.
Papoutsis, I.I., Athanaselis, S.A., Nikolaou, P.D., Pistos, C.M., Spiliopoulou, A., and Maravelias, C.P., J. Pharm. Biomed. Anal., 2010, vol. 52, no. 4, p. 609.
Chèze, M., Villain, M., and Pépin, G., Forensic Sci. Int., 2004, vol. 145, nos. 2–3, p. 123.
Al-Abachi, M.Q. and Hadi, H., Al-Mustansiriyah J. Sci., 2015, vol. 26, no. 1, p. 38.
Hargis, L.G., Analytical Chemistry: Principles and Techniques, New Jersey: Prentice-Hall, 1998.
Inczedy, J., Analytical Application of Complex Equilibria, Budapest: Akademiai Kiado, 1976.
Author information
Authors and Affiliations
Corresponding author
Additional information
The article is published in the original.
Rights and permissions
About this article
Cite this article
Hadi, H., Mouayed, M. Determination of clonazepam in pharmaceutical preparations using simple high-throughput flow injection system. J Anal Chem 72, 226–233 (2017). https://doi.org/10.1134/S1061934817020058
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1061934817020058