Abstract
A method has been developed for monitoring the dynamics of the number and viability of Dunaliella salina microalgae taking into account the spectral contribution from aggregates of metal particles tested as a toxic agent in a microplate assay system. The method is based on the in vivo determination of the chlorophyll content from the intensity of an absorption peak at 680 nm corrected for the nonselective extinction as calculated using the values of nearest local minima at 640 and 740 nm.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Mecozzi, M., Onorati, F., Oteri, F., and Sarni, A., Int. J. Environ. Pollut., 2008, vol. 32, p. 104.
Zakhozhii, I.G., Matalin, D.A., Popova, L.G., and Balnokin, Yu.V., Fiziol. Rastenii, 2012, vol. 59, p. 48.
Rabinovich, E., Fotosintez (Photosynthesis), Moscow: Inostrannaya Literatura, 1953, vol.2.
Fujiki, T. and Taguchi, S., J. Plankton Res., 2002, vol. 24, p. 859.
Kubín, Š., Arch. Protistenkd., 1991, vol. 139, p. 111.
Chekanov, K.A. and Solovchenko, A.E., Fiziol. Rastenii, 2015, vol. 62, p. 291.
Koci, V., Dragoun, D., and Lukavský, J., Algol. Stud., 2006, vol. 122, p. 123.
Arar, E.J., National Exposure Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Cincinnati, Ohio, USA, 1997.
Bogatyrev, V.A., Golubev, A.A., Selivanov, N.Yu., Prilepskii, A.Yu., Bukina, O.G., Pylaev, T.E., Bibikova, O.A., Dykman, L.A., and Khlebtsov, N.G., Nanotechnol. Russ., 2015, vol. 10, nos. 1–2, p. 109.
Golubev, A.A., Prilepskii, A.Y., Dykman, L.A., Khlebtsov, N.G., and Bogatyrev, V.A., Toxicol. Sci., 2016, vol. 151, p. 115.
Khlebtsov, N.G., Dykman, L.A., Krasnov, Ya.M., and Mel’nikov, A.G., Colloid J., 2000, vol. 62, p. 765.
Shaish, A., Avro, M., and Ben-Amotz, A., Plant Cell Physiol., 1990, vol. 31, p. 689.
Frens, G., Nature (London), 1973, vol. 241, p. 20.
Duff, D.G., Baiker, A., and Edwards, P.P., Langmuir, 1993, vol. 9, p. 2301.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © D.S. Chumakov, A.A. Golubev, L.A. Dykman, N.G. Khlebtsov, V.A. Bogatyrev, 2017, published in Kolloidnyi Zhurnal, 2017, Vol. 79, No. 6, pp. 808–812.
Rights and permissions
About this article
Cite this article
Chumakov, D.S., Golubev, A.A., Dykman, L.A. et al. Optical assessment of Dunaliella salina microalgae viability upon toxicological testing. Colloid J 79, 844–848 (2017). https://doi.org/10.1134/S1061933X17060059
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1061933X17060059