Abstract
The Drosophila melanogaster Z3314 line isolated from a Zvenigorodka (Ukraine) natural population is characterized by the manifestation and emergence of a wide spectrum of molecular aberrations. Among them, two types (the wing venation anomaly and violation of the leg segmentation) were the most represented. It was demonstrated that the frequency of manifestation (penetrance) and the expressiveness of these phenotypic aberrations increase with an increase in the temperature. When the Z3314 line is bred in the laboratory, autosomal visible rase (ra: 3–97.3) mutation, which leads to reduction of a part of dorso-central and scutellaria macrochaetae, was detected (isolated and identified). A number of genetic peculiarities that determined the consistency and prospects of the study were found during the mutation process study in the Z3314 line. The Z3314 line is characterized by a high frequency of the emergence of visible mutations in the X-Z3314 chromosome, which persisted for a long time of the breeding under laboratory conditions (from 2003 to 2011). Locus-specific high genetic instability in the singed locus in the X-Z3314 chromosome persisted from the moment of emergence of the first mutant alleles in 2006 until the end of the study. The emergence of mutations was observed both during the line breeding “inside” (in the case of brother–sister crossings) and after the crossings of the X-Z3314 chromosome carrier males with females of the С(1)DX,ywf/Y laboratory line with linked X chromosomes.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Chetverikov, S.S., About some moments in the evolutionary process from the point of view of modern genetics, Zh. Eksp. Boil., Ser. A, 1926, vol. 2, no. 1, pp. 3–54.
Duseeva, N.D., High yellow gene mutability in natural populations of Drosophila melanogaster, Dokl. Akad. Nauk SSSR, 1948, vol. 59, no. 2, pp. 329–331.
Dubinin, N.P., Evolyutsiya populyatsii i radiatsiya (Evolution of Populations and Radiation), Moscow: Atomizdat, 1966.
Berg, R.L., Yellow mutation in the Uman population of Drosophila melanogaster, Vestn. Leningrad. Univ., Ser. Biol., 1961, no. 1, pp. 77–89.
Golubovskii, M.D., Ivanov, Yu.N., Zakharov, I.K., and Berg, R.L., Study of synchronous and parallel changes in gene pools in natural populations of fruit flies, Drosophila melanogaster, Genetika (Moscow), 1974, vol. 10, no. 4, pp. 72–83.
Tinyakov, G.G., Highly mutable line from the wild population of Drosophila melanogaster, Dokl. Akad. Nauk SSSR, 1939, vol. 22, no. 9, p. 615.
Demerec, M., Unstable genes in Drosophila, Cold Spring Harbor Symp. Quant. Biol., 1941, vol. 9, pp. 145–159.
Neel, J.V., A study of a case of high mutation rate in Drosophila melanogaster, Genetics, 1942, vol. 27, pp. 519–536.
Green, M., The genetic of mutable gene at the white locus of Drosophila melanogaster, Genetics, 1967, vol. 56, pp. 467–482.
Zakharov, I.K. and Skibitskii, E.E., Genetics of unstable alleles from genes on the X-chromosome isolated during the outbreak of the yellow mutations in 1982— 1991 in the natural population of Drosophila melanogaster in Uman’, Genetika (Moscow), 1995, vol. 31, no. 8, pp. 1079–1084.
Yurchenko, N.N. and Zakharov, I.K., Mutable XZchromosome isolated from natural population of Drosophila melanogaster, Genetika (Moscow), 1995, vol. 31, no. 3, p. 422–426.
Berg, R.L., A simultaneous mutability rise at the singed locus in two out of three Drosophila melanogaster population studied in 1973, Drosophila Inform. Serv., 1974, no. 51, p. 100.
Golubovskii, M.D., The instability of the singed locus in Drosophila melanogaster: mutant and normal alleles that reverse according to the all-or-nothing principle, Genetika (Moscow), 1977, vol. 13, no. 5, pp. 847–861.
Golubovskii, M.D. and Erokhina, I.D., Mutation process in lines with super-mutable alleles of the singed locus in Drosophila melanogaster, Genetika (Moscow), 1977, vol. 13, no. 7, pp. 1210–1219.
Zakharov, I.K. and Golubovskii, M.D., A series of unstable alleles of the singed gene isolated from natural populations of Drosophila melanogaster: patterns of mutation, Genetika (Moscow), 1984, vol. 20, no. 7, pp. 1117–1124.
Zakharov, I.K., Mutations and mutation process in natural populations of Drosophila melanogaster, Extended Abstract of Doctoral Dissertation, Insitut Tsitologii i Genetiki, Sibirskoe Otdelenie, Rossiiskaya Akademiya Nauk, Novosibirsk, 1995.
Zakharov, I.K., Ivannikov, A.V., Skibitskii, E.E., et al., Genetic properties of alleles from genes on the X-chromosome isolated from natural Drosophila melanogaster populations during mutational outbreak, Dokl. Biol. Sci., 1995, vol. 341, no. 1, pp. 126–129.
Zakharov, I.K., Ivannikov, A.V., and Yurchenko, N.N., Dynamics of the mutational process and the gene pool in Drosophila melanogaster natural populations, Sovremennye kontseptsii evolyutsionnoi genetiki (Modern Concepts of Evolutionary Genetics), Shumnyi, V.K. and Markel’, A.L., Eds., Novosibirsk: Inst. Tsitol. Genet., Sib. Otd. Ross. Akad. Nauk, 2000, pp. 151–159.
Medvedev, N.N., Prakticheskaya genetika (Practical Genetics), Moscow: Nauka, 1966.
Golubovskii, M.D., Zakharov, I.K., and Sokolova, O.A. Analysis of the instability of the yellow gene alleles isolated from the natural population of fruit fly during mutational outbreak, Genetika (Moscow), 1987, vol. 23, no. 9, pp. 1595–1603.
Green, M.M., A case for DNA insertion mutants in Drosophila melanogaster, in DNA Insertion Elements, Plasmids, and Episomes, Bukhari, A.I., Shapiro, J.A., and Adhya, S.L., Eds., New York: Cold Spring Harbor Lab., 1977, pp. 437–445.
Golubovsky, M.D., Ivanov, Y.N., and Green, M.M. Genetic instability in Drosophila melanogaster: putative multiple insertion mutants at the singed bristle locus, Proc. Natl. Acad. Sci. U.S.A., 1977, vol. 74, pp. 2973–2975.
Biessmann, H., Molecular analysis of the yellow gene (y) region of Drosophila melanogaster, Proc. Natl. Acad. Sci. U.S.A., 1985, vol. 82, pp. 7369–7373.
O’Hare, K., Tam, J.L.-Y., Lim, J.K., et al., Rearrangements at a hobo element inserted into the first intron of the singed gene in the unstable sn49 system of Drosophila melanogaster, Mol. Gen. Genet., 1998, vol. 257, pp. 452–460.
Zakharenko, L.P., Zakharov, I.K., Romanova, O.A., et al., “Mode for mutation” in the Uman’ natural population of Drosophila melanogaster is due to the spreading of a hobo-induced unstable inversion in the regulatory region of the yellow gene, Russ. J. Genet., 2000, vol. 36, no. 6, pp. 603–610.
Engels, W.R., Extrachromosomal control of mutability in Drosophila melanogaster, Proc. Natl. Acad. Sci. U.S.A., 1979, vol. 76, no. 8, pp. 4011–4015.
Gracheva, E.M., Zakharov, I.K., Voloshina, M.A., et al., Mutation bursts at the yellow locus in a natural population of Drosophila melanogaster are associated with a hobo insertion, Russ. J. Genet., 1998, vol. 34, no. 4, pp. 364–370.
Lindsley, D.L. and Zimm, G.G., The Genome of Drosophila melanogaster, New York: Acad. Press, 1992.
http://flybase.org.
Yurchenko, N.N., O’Hare, K., and Zakharov, I.K., Unstable system sn49 in Drosophila melanogaster: analysis by blot-hybridization and polymerase chain reaction, Russ. J. Genet., 1996, vol. 32, no. 5, pp. 533–539.
Ives, P.T., The importance of mutation rate genes in evolution, Evolution, 1950, vol. 4, no. 3, pp. 236–252.
Voloshina, M.A. and Golubovskii, M.D., Genetic analysis of chromosome and cytoplasmic effects of the R-M hybrid dysgenesis system on the instability of mutations caused by the introduction of various mobile elements in Drosophila, Genetika (Moscow), 1986, vol. 22, no. 4, pp. 624–632.
Golubovskii, M.D., Voloshina, M.A., Zakharov, I.K., and Yurchenko, N.N. The relationship between P and mdg3 mobile elements in Drosophila melanogaster: the genetic aspect, Genetika (Moscow), 1986, vol. 22, no. 10, pp. 2452–2458.
Zakharov, I.K., Genetics of natural populations of Drosophila melanogaster: variation in mutability and concentration of the singed gene alleles in natural populations, Genetika (Moscow), 1984, vol. 20, no. 8, pp. 1295–1304.
Yurchenko, N.N., Zakharov, I.K., and Golubovskii, M.D. Influence of the homologous locus structure on the behavior of the Tn::clw transposon in Drosophila melanogaster, Genetika (Moscow), 1985, vol. 21, no. 8, pp. 1290–1297.
Yurchenko, N.N., Koryakov, D.E., and Zakharov, I.K. Appearance of recessive lethal mutations in derivatives of the unstable XZ chromosome of Drosophila melanogaster, Genetica (Moscow), 1995, vol. 31, no. 9, pp. 1218–1224.
Golubovskii, M.D. and Zakharov, I.K., Insertional mutagenesis in natural populations of Drosophila, in Uspekhi teoreticheskoi i prikladnoi genetiki (Advances in Theoretical and Applied Genetics), Novosibirsk: Inst. Tsitol. Genet., Sib. Otd. Akad. Nauk SSSR, 1982, pp. 180–182.
Rutherford, S.L. and Lindquist, S., Hsp90 as a capacitor for morphological evolution, Nature, 1998, vol. 396, pp. 336–342.
Kidwell, M.G., Kidwell, J., and Sved, J. Hybrid dysgenesis in Drosophila melanogaster: a syndrome of aberrant traits including mutation, sterility and male recombination, Genetics, 1977, vol. 86, pp. 813–833.
Kovalenko, L.V., Zakharenko, L.P., and Zakharov, I.K. Transposition of the hobo element in Drosophila melanogaster somatic cells, Russ. J. Genet., 2006, vol. 42, no. 2, pp. 122–128. https://doi.org/10.1134/S1022795- 406020049.
Green, M.M., Todo, T., Ryo, H., and Fujikawa, K. Genetic-molecular basis for a simple Drosophila melanogaster somatic system that detects environmental mutagens, Proc. Natl. Acad. Sci. U.S.A., 1986, vol. 83, pp. 6667–6671.
Tcheressiz, S., Calco, V., Arnaud, F., et al., Expression of the Idefix retrotransposon in early follicle cells in the germarium of Drosophila melanogaster determined by its LTR sequence and a specific genomic context, Mol. Genet. Genomics, 2002, vol. 267, no. 2, pp. 133–141.
Morozova, T.V., Tcybulko, E.A., and Pasyukova, E.G. Regulatory elements of the copia retrotransposon determine different levels of expression in different organs of males and females of Drosophila melanogaster, Russ. J. Genet., 2009, vol. 45, p. 147.
David, J.R. and Capy, P., Genetic variation of Drosophila melanogaster natural populations, Trends Genet., 1988, vol. 4, no. 4, pp. 106–111.
Kidwell, M.G. and Lisch, D.R., Transposable elements as sources of genomic variation, in Mobile DNA II, 2002, pp. 59–93.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © Yu.A. Koromyslov, Yu.Yu. Ilinsky, A.V. Ivannikov, I.K. Zakharov, 2018, published in Genetika, 2018, Vol. 54, No. 2, pp. 167–176.
Rights and permissions
About this article
Cite this article
Koromyslov, Y.A., Ilinsky, Y.Y., Ivannikov, A.V. et al. Peculiarities of Mutation Process in X Chromosome of Drosophila melanogaster Z3314 Line from Zvenigorodka (Ukraine) Natural Population. Russ J Genet 54, 157–165 (2018). https://doi.org/10.1134/S1022795418020114
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795418020114