Abstract
Using high throughput sequencing of the nodA gene, we studied the population dynamics of Rhizobium leguminosarum (bv. viciae, bv. trifolii) in rhizospheric and nodular subpopulations associated with the leguminous plants representing different cross-inoculation groups (Vicia sativa, Lathyrus pratensis of the vetch/vetchling/pea group and Trifolium hybridum of the clover group). The “rhizosphere → nodules” transitions result in either an increase or decrease in the frequencies of 10 of the 23 operational taxonomic units (OTUs) (which were identified with 95% similarity) depending on the symbiotic specificity and phylogenetic positions of OTUs. Statistical and bioinformatical analysis of the population structures suggest that the type of natural selection responsible for these changes may be diversifying at the whole-population level and frequency-dependent at the OTU-specific level, ensuring the divergent evolution of rhizobia interacting with different host species.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Provorov, N.A. and Vorob’ev, N.I., Geneticheskie osnovy evolyutsii rastitel’no-mikrobnogo simbioza (Evolutionary Genetics of Plant–Microbe Symbiosis), Tikhonovich, I.A., Ed., St. Petersburg: Inform-Navigator, 2012.
Wang, D., Yang, S., Tang, F., and Zhu, H., Symbiosis specificity in the legume-rhizobial mutualism, Cell. Microbiol., 2012, vol. 14, no. 3, pp. 334–342.
Provorov, N.A., Tsyganova, A.V., Brewin, N.J., et al., Evolution of symbiotic bacteria within the extraand intra-cellular plant compartments: experimental evidence and mathematical simulation (mini-review), Symbiosis, 2012, vol. 58, nos. 1–3, pp. 39–50.
Andronov, E.E., Onishchuk, O.P., Kurchak, O.N., and Provorov, N.A., Population structure of the clover rhizobia Rhizobium leguminosarum bv. trifolii upon transition from soil into the nodular niche, Microbiology (Moscow), 2014, vol. 83, no. 4, pp. 422–429.
Nei, M., Estimation of average heterozygosity and genetic distance from a small number of individuals, Genetics, 1978, vol. 89, no. 4, pp. 583–590.
Selander, R.K., Caugant, D.A., Ochman, H., et al., Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics, Appl. Environ. Microbiol., 1986, vol. 51, no. 5, pp. 873–884.
Lakin, G.F., Biometriya (Biometry), Moscow: Vysshaya Shkola, 1990.
Vorobyov, N.I. and Provorov, N.A., Simulation of legume–Rhizobium symbiosis evolution under multistrain competition among bacteria for inoculation of symbiotic niches, Ekol. Genet., 2008, vol. 6, no. 4, pp. 3–11.
Provorov, N.A. and Tikhonovich, I.A., Genetic and molecular bases of symbiotic adaptations, Usp. Sovrem. Biol., 2014, vol. 134, no. 3, pp. 211–226.
Shlaman, Kh., Fillips, D., and Kondoroshi, E., Genetic organization and transcriptional regulation of rhizobia genes controlling nodulation, in Rhizobiaceae: molekulyarnaya biologiya bakterii, vzaimodeistvuyushchikh s rasteniyami (Rhizobiaceae: Molecular Biology of Bacteria Interacting with Plants), Spaink, G., Kondoroshi, A., and Khukas, P., Eds., St. Petersburg: Biont, 2002, pp. 389–416.
Guttman, D.S., Gropp, S.J., Morgan, R.L., and Wang, P.W., Diversifying selection drives the evolution of the type III secretion system pilus of Pseudomonas syringae, Mol. Biol. Evol., 2006, vol. 23, no. 12, pp. 2342–2354.
Timofeev-Resovskii, N.V., Vorontsov, N.N., and Yablokov, A.V., Kratkii ocherk teorii evolyutsii (Short Essay on the Theory of Evolution), Moscow: Nauka, 1977.
Provorov, N.A., Andronov, E.E., Onishchuk, O.P., et al., Genetic structure of the introduced and local populations of Rhizobioum leguminosarum in plant–soil systems, Microbiology (Moscow), 2012, vol. 81, no. 2, pp. 224–232.
Trotter, M.V. and Spencer, H.G., Frequency-dependent selection and the maintenance of genetic variation: exploring the parameter space of the multiallelic pairwise interaction model, Genetics, 2007, vol. 176, no. 3, pp. 1729–1740.
Friesen, M.L., Saxer, G., Travisano, M., and Doeveli, M., Experimental evidence for sympartric ecological diversification due to frequency-dependent competition in Escherichia coli, Evolution, 2004, vol. 58, no. 2, pp. 245–260.
Provorov, N.A. and Vorob’ev, N.I., Microevolution of nodule bacteria upon generation of mutants with altered survival in the plant–soil system, Russ. J. Genet., 2003, vol. 39, no. 12, pp. 1349–1359.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © E.E. Andronov, A.A. Igolkina, A.K. Kimeklis, N.I. Vorobyov, N.A. Provorov, 2015, published in Genetika, 2015, Vol. 51, No. 10, pp. 1108–1116.
Rights and permissions
About this article
Cite this article
Andronov, E.E., Igolkina, A.A., Kimeklis, A.K. et al. Characteristics of natural selection in populations of nodule bacteria (Rhizobium leguminosarum) interacting with different host plants. Russ J Genet 51, 949–956 (2015). https://doi.org/10.1134/S1022795415100026
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795415100026