Abstract
We characterized AA1 (Abscisic acid Activated 1), a protein from Lupinus luteus L. predicted to be located in the apoplastic space who’s mRNA and protein levels are strongly regulated by ABA, salt stress, and hypothermia. A fragment from the recombinant AA1 protein binds ABA as shown by the spectrofluorimetric titration assay of the protein by ABA. The BLAST software of the DFCI database identified more than 200 ESTs from 46 dicots and monocots, including three genes with unknown function from Arabidopsis thaliana, which are closely related to the lupine AA1. The central part of the proteins encoded by these genes contains the TolB motif from Escherichia coli and shares conserved WD40-like repeats, which form the basis for the tertiary beta-propeller structure and provide a potential platform for the assembly of protein complexes. Our data suggest that the highly conserved AA1 proteins from L. luteus and other higher plants are involved in ABA-mediated responses.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- AA1 :
-
Abscisic acid Activated 1
- Ab:
-
antibodies
- BA:
-
benzyladenine
- DPP IV:
-
IV dipeptidyl peptidases
- FITC:
-
fluorescein isothiocyanate
- IgG:
-
immunoglobulins
- 154N-AA1 :
-
coding sequence of AA1 gene in vector pQE-30 including the sequence encoding the 6xHis and additional 5 amino acids from the pQE-30 vector
References
Wasilewska A., Vlad F., Sirichandra C., Redko Y., Jammes F., Valon C., Frei dit Frey N., and Leung J., An update on abscisic acid signaling in plants and more, Mol. Plant, 2008, vol. 1, pp. 198–217.
Schroeder, J.I., Kwak, J.M., and Allen, G.J., Guard cell abscisic acid signalling and engineering drought hardiness in plants, Nature, 2001, vol. 410, pp. 327–330.
MacRobbie, E.C., ABA-induced ion efflux in stomatal guard cells: multiple actions of ABA inside and outside the cell, Plant J., 1995, vol. 7, pp. 565–576.
Novikova, G.V., Stepanchenko, N.S., Nosov, A.V., and Moshkov, I.E., At the beginning of the route: ABA perception and signal transduction in plants, Russ. J. Plant Physiol., 2009, vol. 56, pp. 727–741.
Melcher, K., Ng, L.M., Zhou, E., Soon, F.F., Xu, Y., Suino-Powell, K.M., Park, S.Y., Weiner, J.J., Fujii, H., Chinnusamy, V., Kovach, A., Li, J., Wang, Y., Li, J., Peterson, F.C., Jensen, D.R., Yong, E.L., Volkman, B.F., Cutler, S.R., Zhu, J.K., and Xu, H.E., A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors, Nature, 2009, vol. 462, pp. 602–608.
Fujii, H., Chinnusamy, V., Rodrigues, A., Rubio, S., Antoni, R., Park, S.Y., Cutler, S.R., Sheen, J., Rodriguez, P.L., and Zhu, J.K., In vitro reconstitution of an abscisic acid signalling pathway, Nature, 2009, vol. 462, pp. 660–664.
Joshi-Saha, A., Valon, C., and Leung, J., A brand new START: abscisic acid perception and transduction in the guard cell, Sci. Signal., 2011, vol. 4: re4, doi 10.1126/scisignal.2002164
Pandey, S., Nelson, D.C., and Assmann, S.M., Two novel GPCR-type G proteins are abscisic acid receptors in Arabidopsis, Cell, 2009, vol. 136, pp. 136–148.
Raghavendra, A.S., Gonugunta, V.K., Christmann, A., and Grill, E., ABA perception and signalling, Trends Plant Sci., 2010, vol. 15, pp. 395–401.
Finkelstein, R., Studies of abscisic acid perception finally flower, Plant Cell, 2006, vol. 18, pp. 786–791.
Sambrook, J. Fritsch, E.F., and Maniatis, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, N.Y.: Cold Spring Harbor Lab. Press, 1989, vol. 1, ISBN 0-87969-309-6
Liang, P., Bauer, D., Averboukh, L., Warthoe, P., Rohrwild, M., Muller, H., Strauss, M., and Pardee, A.B., Analysis of altered gene expression by differential display, Methods Enzymol., 1995, vol. 254, pp. 304–321.
Egorov, A.M., Osipov, A.P., Dzantiev, B.B., and Gavrilova, E.M., Teoriya i praktika immunofermentnogo analiza (Theory and Practice of Immunoassay), Moscow: Vyssh. Shkola, 1999.
Conlon, H.E. and Salter, M.G., Plant protein extraction, Methods Mol. Biol., 2007, vol. 362, pp. 379–383.
Stoscheck, C.M., Quantitation of protein, Methods Enzymol., 1990, vol. 182, pp. 50–69.
Timmons, T. and Dunbar, B., Protein blotting and immunodetection, Methods Enzymol., 1990, vol. 182, pp. 679–688.
Permyakov, E.A., Luminescent Spectroscopy of Proteins, Boca Raton, Ann Arbor, London, Tokyo: CRC Press, 1993.
Bogoeva, V.P., Radeva, M.A., Atanasova, L.Y., Stoitsova, S.R., and Boteva, R.N., Fluorescence analysis of hormone binding activities of wheat germ agglutinin, Biochim. Biophys. Acta, 2004, vol. 1698, pp. 213–218.
Pasternak, O., Bujacz, G.D., Fujimoto, Y., Hashimoto, Y., Jelen, F., Otlewski, J., Sikorski, M.M., and Jaskolski, M., Crystal structure of Vigna radiate cytokinin-specific binding protein in complex with zeatin, Plant Cell, 2006, vol. 18, pp. 2622–2634.
Marquardt, D.W., An algorithm for least-squares estimation of nonlinear parameters, J. Soc. Industr. Appl. Mathematics, 1963, vol. 11, pp. 431–441.
Kusnetsov, V.V., Oelmüller, R., Sarwat, M.I., Porfirova, S.A., Cherepneva, G.N., Herrmann, R.G., and Kulaeva, O.N., Cytokinins, abscisic acid and light affect accumulation of chloroplast proteins in Lupinus luteus cotyledons without notable effect on steadystate mRNA levels, Planta, 1994, vol. 194, pp. 318–327.
Santiago, J., Rodrigues, A., Saez, A., Rubio, S., Antoni, R., Dupeux, F., Park, S.Y., Märquez, J.A., Cutler, S.R., and Rodriguez, P.L., Modulation of drought resistance by the abscisic acid receptor PYL5 through inhibition of clade A PP2Cs, Plant J., 2009, vol. 60, pp. 75–88.
Li, G., Xin, H., Zheng, X.F., Li, S., and Hu, Z., Identification of the abscisic acid receptor VvPYL1 in Vitis vinifera, Plant Biol., 2012, vol. 14, pp. 244–248.
Smith, T.F., Gaitatzes, C., Saxena, K., and Neer, E.J., The WD repeat: a common architecture for diverse functions, Trends Biochem. Sci., 1999, vol. 24, pp. 181–185.
Shirameti, I., Shahollari, B., Landsberger, M., Westermann, M., Cherepneva, G., Kusnetsov, V., and Oelmüller, R., Cytokinin stimulates polyribosome loading of nuclear-encoded mRNAs for the plastid ATP synthase in etiolplasts of Lupinus luteus: the complex accumulates in the inner envelope membrane with the CF1 moiety located towards the stromal space, Plant J., 2004, vol. 38, pp. 578–593.
Xu, Y.H., Liu, R., Yan, L., Liu, Z.Q., Jiang, S.C., Shen, Y.Y., Wang, X.F., and Zhang, D.P., Light-harvesting chlorophyll a/b-binding proteins are required for stomatal response to abscisic acid in Arabidopsis, J. Exp. Bot., 2012, vol. 63, pp. 1095–1106.
Leung, J. and Giraudat, J., Abscisic acid signal transduction, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1998, vol. 49, pp. 199–222.
Zeevaart, J.A. and Creelman, R.A., Metabolism and physiology of abscisic acid, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1988, vol. 39, pp. 439–473.
Cornish, K. and Zeevaart, J.A.D., Abscisic acid accumulation by in situ and isolated guard cells of Pisum sativum L. and Vicia faba L. in relation to water stress, Plant Physiol., 1986, vol. 81, pp. 1017–1021.
Boudart, G., Jamet, E., Rossignol, M., Lafitte, C., Borderies, G., Jauneau, A., Esquerre-Tugaye, M.T., and Pont-Lezica, R., Cell wall proteins in apoplastic fluids of Arabidopsis thaliana rosettes: identification by mass spectrometry and bioinformatics, Proteomics, 2005, vol. 5, pp. 212–221.
Gardiner, E.E., Al-Tamimi, M., Andrews, R.K., and Berndt, M.C., Platelet receptor shedding, Methods Mol. Biol., 2012, vol. 788, pp. 321–339.
Higashiyama, S., Nanba, D., Nakayama, H., Inoue, H., and Fukuda, S., Ectodomain shedding and remnant peptide signaling of EGFRs and their ligands, J. Biochem., 2011, vol. 150, pp. 15–22.
Author information
Authors and Affiliations
Corresponding author
Additional information
This text was submitted by authors in English.
Electronic supplementary material: The online version of this article contains supplementary material, which is available to authorized users.
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Demidenko, A.V., Kudryakova, N.V., Karavaiko, N.N. et al. The ABA-binding protein AA1 of Lupinus luteus is involved in ABA-mediated responses. Russ J Plant Physiol 62, 161–170 (2015). https://doi.org/10.1134/S1021443715020053
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1021443715020053