Abstract
In this paper, mesoporous silica with large specific surface area was used to immobilize laccase by the glutaraldehyde cross-linking method, and after screening and optimization experiments, the best enzyme immobilization process conditions were found (25°C, pH 5.4, 4% glutaraldehyde and 0.2 g/L laccase, treatment time 6 h). After that, the removal and degradation ratio of 2,4-dichlorophenol (abbreviated as DCP) under different conditions were also studied. After the degradation process was performed for 6 h at 30°C, pH 5.4, and DCP initial concentration of 50 mg/L in the presence of 0.1 g of immobilized laccase, the removal ratio and the degradation ratio were 42.28 and 15.93%, respectively. Compared with free laccase, the reusability of immobilized laccase is significantly improved.
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Yang, Y., Xu, Y., Yang, Y. et al. Laccase immobilized on mesoporous SiO2 and its use for degradation of chlorophenol pesticides. Russ. J. Phys. Chem. 90, 2044–2054 (2016). https://doi.org/10.1134/S0036024416100307
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DOI: https://doi.org/10.1134/S0036024416100307