Abstract
Although dryland ecosystems are the most abundant terrestrial biomes on the Earth, relatively little is known about their microbial diversity and potential metabolic activities. Therefore, the bacterial diversity of the Lut Desert in Iran has been remained largely obscure. In this study, ionizing radiation resistant bacteria from arid Gandom Beryan region was investigated by a culture-dependent method. After exposing the soil and surface sand samples to different periods of dehydration in a desiccator containing silica gel, two nonendospore- forming bacterial isolates were recovered by plating on R2A and TSA agar media and then subjected to a desiccation and ionizing radiation resistance assay. The isolates A2 and B9 were still recovered after 8 weeks in a desiccator containing silica gel and were moderately resistant to gamma radiation with a D10 value between 2 and 4 kGy. Strains A2 and B9 were affiliated with Modestobacter muralis MDVD1T (99.7% similarity) and Maritalea mobilis E6T (97.3% similarity) respectively, using 16S rRNA gene sequence analysis. This is the first report of radiation resistant bacteria which belongs to the genus Maritalea.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Azua-Bustos, A., Urrejola, C., and Vicuna, R., Life at the dry edge: microorganisms of the Atacama Desert, FEBS Lett., 2012, vol. 586, no. 18, pp. 2939–2945.
Billi, D., Friedmann, E.I., Hofer, K.G., Caiola, M.G., and Ocampo-Friedmann, R., Ionizing-radiation resistance in the desiccation-tolerant cyanobacterium Chroococcidiopsis, Appl. Environ. Microbiol., 2000, vol. 66, no. 4, pp. 1489–1492.
Bull, A.T. and Asenjo, J.A., Microbiology of hyper-arid environments: recent insights from the Atacama Desert, Chile, Antonie van Leeuwenhoek, 2013, vol. 103, pp. 1173–1179.
Busarakam, K., Bull, A.T., Trujillo, M.E., Riesco, R., Sangal, V., Van Wezel, G.P., and Goodfellow, M., Modestobacter caceresii sp. nov., novel actinobacteria with an insight into their adaptive mechanisms for survival in extreme hyper-arid Atacama Desert soils, Syst. Appl. Microbiol., 2016, vol. 39, pp. 243–251.
Carter, M.R., and Gregorich, E.G., Soil Sampling and Methods of Analysis, 2007, CRC.
Chanal, A., Chapon, V., Benzerara, K., Barakat, M., Christen, R., Achouak, W., Barras, F., and Heulin, T., The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria, Environ. Microbiol., 2006, vol. 8, pp. 514–525.
Chen, M., and Alexander, M., Survival of soil bacteria during prolonged desiccation, Soil. Biol. Biochem., 1973, vol. 5, no. 2, pp. 213–221.
Felsenstein, J., Confidence limits on phylogenies: an approach using bootstrap, Evolution., 1985, vol. 39, pp. 783–791.
Fredrickson, J.K., Li, S.M., Gaidamakova, E.K., Matrosova, V.Y., Zhai, M., Sulloway, H.M., Scholten, J.C., Brown, M.G., Balkwill, D.L., and Daly, M.J., Protein oxidation: key to bacterial desiccation resistance?, ISME J., 2008, vol. 2, pp. 393–403.
Fukui, Y., Abe, M., Kobayashi, M., Ishihara, K., Oikawa, H., Yano, Y., and Satomi, M., Maritalea porphyrae sp. nov., isolated from a red alga (Porphyra yezoensis), and transfer of Zhangella mobilis to Maritalea mobilis comb. nov., Int. J. Syst. Evol. Microbiol., 2012, vol. 62, pp. 43–48.
Gtari, M., Essoussi, I., Maaoui, R., Sghaier, H., Boujmil, R., Gury, J., Pujic, P., Brusetti, L., Chouaia, B., Crotti, E., Daffonchio, D., Boudabous, A., and Normand, P., Contrasted resistance of stone-dwelling Geodermatophilaceae species to stresses known to give rise to reactive oxygen species, FEMS Microbiol. Ecol., 2012, vol. 80, pp. 566–577.
Luedemann, G.M., Geodermatophilus, a new genus of the Dermatophilaceae (Actinomycetales), J. Bacteriol., 1968, vol. 96, pp. 1848–1858.
Mattimore, V. and Battista, J.R., Radioresistance of Deinococcus radiodurans: functions necessary to survive ionizing radiation are also necessary to survive rolonged desiccation, J. Bacteriol., 1996, vol. 178, pp. 633–637.
Mohseni, M., Abbaszadeh, J, and Nasrollahi Omran, A., Radiation resistant of native Deinococcus spp. isolated from the Lout desert of Iran “the hottest place on Earth”, Int. J. Environ. Sci. Technol., 2014, vol. 11, pp. 1939–1946.
Rainey, F.A., Ray, K., Ferreira, M., Gatz, B.Z., Nobre, M.F., Bagaley, D., Rash, B.A., Park, M.J., Earl, A.M., Shank, N.C., Small, A.M., Henk, M.C., Battista, J.R., Kämpfer, P., and da Costa, M.S., Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample, Appl. Environ. Microbiol., 2005, vol. 71, pp. 5225–5235.
Saitou, N. and Nei, M., The neighbor-joining method: a new method for reconstructing phylogenetic trees, Mol. Biol. Evol., 1987, vol. 4, pp. 406–425.
Shirsalimian, M.S., Amoozegar, M.A., Akhavan Sepahy, A., Kalantar, S.M., and Dabbagh, R., Isolation of extremely halophilic Archaea from a saline river in the Lut Desert of Iran, moderately resistant to desiccation and gamma radiation, Microbiology (Moscow), 2017, vol. 86, pp. 403–411.
Slade, D. and Radman, M., Oxidative stress resistance in Deinococcus radiodurans, Microbiol. Mol. Biol. Rev., 2011, vol. 75, pp. 133–191.
Spencer, J.F.T. and Ragout de Spencer, A.L., Environmental Microbiology: Methods and Protocols, Humana, 2004.
Sun, H.M., Zhang, T., Yu, L.Y., Sen, K., and Zhang, Y.Q., Ubiquity, diversity and physiological characteristics of Geodermatophilaceae in Shapotou National Desert Ecological Reserve, Front. Microbiol., 2015, vol. 6.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S., MEGA6: molecular evolutionary genetics analysis version 6.0., Mol. Biol. Evol., 2013, vol. 30, pp. 2725–2729.
Tanaka, M., Earl, A.M., Howell, H.A., Park, M.J., Eisen, J.A., Peterson, S.N., and Battista, J.R., Analysis of Deinococcus radiodurans’s transcriptional response to ionizing radiation and desiccation reveals novel proteins that contribute to extreme radioresistance, Genetics., 2004, vol. 168, no. 1, pp. 21–33.
Terato, H., Suzuki, K., Nishioka, N., Okamoto, A., Shimazaki-Tokuyama, Y., Inoue, Y., and Saito, T., Characterization and radio-resistant function of manganese superoxide dismutase of Rubrobacter radiotolerans, J. Radiat. Res., 2011, vol. 52, no. 6, pp. 735–742.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.J., The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools, Nucleic. Acids. Res., 1997, vol. 25, pp. 4876–4882.
Xu, H.Y., Chen, L.P., Fu, S.Z., Fan, H.X., Zhou, Y.G., Liu, S.J., and Liu, Z.P., Zhangella mobilis gen. nov., sp. nov., a new member of the family Hyphomicrobiaceae isolated from coastal seawater, Int. J. Syst. Evol. Microbiol., 2009, vol. 59, pp. 2297–2301.
Author information
Authors and Affiliations
Corresponding author
Additional information
The article is published in the original.
Rights and permissions
About this article
Cite this article
Shirsalimian, M.S., Akhavan Sepahy, A., Amoozegar, M.A. et al. Isolation of Two Radiation Resistant and Desiccation Tolerant Bacteria, Modestobacter sp. A2 and Maritalea sp. B9, from Gandom Beryan Hill in the Lut Desert of Iran. Microbiology 87, 363–371 (2018). https://doi.org/10.1134/S0026261718030104
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0026261718030104