Abstract
The endogenous gaseous transmitters (GTs) —nitrogen oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S)—make up a special neuromodulatory system which mediates the development, maturation and plastic modification of nervous centers. We addressed immunolocalization of the key enzymes of GT synthesis, cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), heme oxygenase-2 (HO-2) and constitutive NO synthase (nNOS), at different ontogenetic stages of the human fetal retina. CBS, CSE and HO-2 were found to be expressed in photoreceptor, bipolar and amacrine neurons, the number of which increases in the first and decreases in the third trimesters of gestation. The number of nNOS-immunopositive amacrine and ganglion neurons demonstrates inverse dynamics with maximal values in the third trimester. Uneven distribution patterns of the marker enzymes are discussed in the light of the modulatory function of GTs in neurogenesis of the human retina and their involvement in cytoprotective mechanisms.
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Russian Text © The Author(s), 2019, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2019, Vol. 55, No. 3, pp. 199–207.
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The material was obtained at the Vladivostok Pathological Anatomy Bureau according to the orders of the Ministry of Health of the Russian Federation no. 354n of 06.06.2013 “On conducting pathological autopsies” and no. 179n of 24.03.2016 “On the regulations for conducting pathological anatomical studies”, and the order of the Ministry of Health and Social Development of the Russian Federation no. 346n of 12.05.2010 “On the organization and conducting forensic medical expertise in state forensic examination institutions of the Russian Federation”.
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Matveeva, N.Y., Kalinichenko, S.G., Kotsyuba, E.P. et al. Immunolocalization of Cystathionine β-Synthase, Cystathionine γ-Lyase, Heme Oxygenase-2 and Nitric Oxide Synthase in the Human Fetal Retina in Different Gestational Trimesters. J Evol Biochem Phys 55, 222–230 (2019). https://doi.org/10.1134/S0022093019030074
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DOI: https://doi.org/10.1134/S0022093019030074