Abstract
EPR spin trapping and EPR oximetry were used to study the superoxide radical generation in heart mitochondria from Wistar rats at various oxygen concentrations. Lithium phthalocyanine and TEMPONE-15N-D16 were chosen to determine the oxygen content in a gas-permeable capillary containing mitochondria. TIRON was used as a spin trap. Several oxygen concentrations in the incubation mixture were tested; heart mitochondria were found to generate superoxide in complex III at various partial pressures of oxygen, including deep hypoxia (<5% O2). Dinitrosyl iron complexes with glutathione (the drug Oxacom) exerted an antioxidant effect regardless of the partial pressure of oxygen; the magnitude and kinetic characteristics of the effect depended on the drug concentration.
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Abbreviations
- DNIC:
-
dinitrosyl iron complex
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Original Russian Text © A.L. Dudylina, M.V. Ivanova, K.B. Shumaev, E.K. Ruuge, 2016, published in Biofizika, 2016, Vol. 61, No. 2, pp. 304–309.
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Dudylina, A.L., Ivanova, M.V., Shumaev, K.B. et al. The generation of superoxide radicals by complex III in heart mitochondria and the antioxidant effect of dinitrosyl iron complexes at different partial pressures of oxygen. BIOPHYSICS 61, 257–261 (2016). https://doi.org/10.1134/S0006350916020032
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DOI: https://doi.org/10.1134/S0006350916020032