Abstract
Capsular polysaccharide (CPS) assigned to the K93 type was isolated from the bacterium Acinetobacter baumannii B11911 and studied by sugar analysis along with oneand two-dimensional 1H and 13C NMR spectroscopy. The CPS was found to contain a derivative of pseudaminic acid, and the structure of the branched tetrasaccharide repeating unit was established. Genes in the KL93 capsule biosynthesis locus were annotated and found to be consistent with the CPS structure established. The K93 CPS has the α-D-Galp-(1→6)-β-D-Galp-(1→3)-D-GalpNAc trisaccharide fragment in common with the K14 CPS of Acinetobacter nosocomialis LUH 5541 and A. baumannii D46. It also shares the β-D-Galp-(1→3)-DGalpNAc disaccharide fragment and the corresponding predicted Gal transferase Gtr5, as well as the initiating GalNAc-1P transferase ItrA2, with a number of A. baumannii strains.
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Abbreviations
- COSY:
-
correlation spectroscopy
- CPS:
-
capsular polysaccharide
- HMBC:
-
heteronuclear multiple-bond correlation
- HSQC:
-
heteronuclear single-quantum coherence
- Pse:
-
pseudaminic acid
- RHb:
-
(R)-3-hydroxybutanoyl
- ROESY:
-
rotating-frame nuclear Overhauser effect spectroscopy
- TOCSY:
-
total correlation spectroscopy
References
Roca, I., Espinal, P., Vila-Farres, X., and Vila, J. (2012) The Acinetobacter baumannii oxymoron: commensal hospital dweller turned pan-drug-resistant menace, Front. Microbiol., 3, article148.
Kenyon, J. J., and Hall, R. M. (2013) Variation in the complex carbohydrate biosynthesis loci of Acinetobacter baumannii genomes, PLoS One, 3, e62160.
Russo, T. A., Luke, N. R., Beanan, J. M., Olson, R., Sauberan, S. L., MacDonald, U., Schultz, L. W., Umland, T. C., and Campagnari, A. A. (2010) The K1 capsular polysaccharide of Acinetobacter baumannii strain 307–0294 is a major virulence factor, Infect. Immun., 78, 3993–4000.
Balaji, V., Rajenderan, S., Anandan, S., and Biswas, I. (2015) Genome sequences of two multidrug-resistant Acinetobacter baumannii clinical strains isolated from Southern India, Genome Announc., 3, e01010–e01015.
Westphal, O., and Jann, K. (1965) Bacterial lipopolysaccharides. Extraction with phenol–water and further applications of the procedure, Methods Carbohydr. Chem., 5, 83–91.
Leontein, K., and Lönngren, J. (1993) Determination of the absolute configuration of sugars by gas-liquid chromatography of their acetylated 2-octylglycosides, Methods Carbohydr. Chem., 9, 87–89.
Shashkov, A. S., Paramonov, N. A., Veremeychenko, S. N., Grosskurth, H., Zdorovenko, G. M., Knirel, Y. A., and Kochetkov, N. K. (1998) Somatic antigens of pseudomonads: structure of the O-specific polysaccharide of Pseudomonas fluorescens biovar B, strain IMV 247, Carbohydr. Res., 306, 297–303.
Senchenkova, S. N., Shashkov, A. S., Popova, A. V., Shneider, M. M., Arbatsky, N. P., Miroshnikov, K. A., Volozhantsev, N. V., and Knirel, Y. A. (2015) Structure elucidation of the capsular polysaccharide of Acinetobacter baumannii AB5075 having the KL25 capsule biosynthesis locus, Carbohydr. Res., 408, 8–11.
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. (1990) Basic local alignment search tool, J. Mol. Biol., 215, 403–410.
Knirel, Y. A., Shashkov, A. S., Tsvetkov, Y. E., Jansson, P.E., and Zähringer, U. (2003) 5,7-Diamino-3,5,7,9tetradeoxynon-2-ulosonic acids in bacterial glycopolymers: chemistry and biochemistry, Adv. Carbohydr. Chem. Biochem., 58, 371–417.
Knirel, Y. A., Kocharova, N. A., Shashkov, A. S., Dmitriev, B. A., Kochetkov, N. K., Stanislavsky, E. S., and Mashilova, G. M. (1987) Somatic antigens of Pseudomonas aeruginosa. The structure of the O-specific polysaccharide chains of the lipopolysaccharide from P. aeruginosa O5 (Lanyi) and immunotype 6 (Fisher), Eur. J. Biochem., 163, 639–652.
Knirel, Y. A., Vinogradov, E. V., Shashkov, A. S., Dmitriev, B. A., Kochetkov, N. K., Stanislavsky, E. S., and Mashilova, G. M. (1986) Somatic antigens of Pseudomonas aeruginosa. The structure of O-specific polysaccharide chains of P. aeruginosa O10 (Lányi) lipopolysaccharides, Eur. J. Biochem., 157, 129–138.
Knirel, Y. A., Vinogradov, E. V., Shashkov, A. S., Dmitriev, B. A., Kochetkov, N. K., Stanislavsky, E. S., and Mashilova, G. M. (1987) Somatic antigens of Pseudomonas aeruginosa. The structure of the O-specific polysaccharide chain of the lipopolysaccharide from P. aeruginosa O13 (Lányi), Eur. J. Biochem., 163, 627–637.
Lipkind, G. M., Shashkov, A. S., Knirel, Y. A., Vinogradov, E. V., and Kochetkov, N. K. (1988) A computer-assisted structural analysis of regular polysaccharides on the basis of 13C-n.m.r. data, Carbohydr. Res., 175, 59–75.
Senchenkova, S. N., Popova, A. V., Shashkov, A. S., Shneider, M. M., Mei, Z., Arbatsky, N. P., Liu, B., Miroshnikov, K. A., Volozhantsev, N. V., and Knirel, Y. A. (2015) Structure of a new pseudaminic acid-containing capsular polysaccharide of Acinetobacter baumannii LUH5550 having the KL42 capsule biosynthesis locus, Carbohydr. Res., 407, 154–157.
Senchenkova, S. N., Shashkov, A. S., Shneider, M. M., Arbatsky, N. P., Popova, A. V., Miroshnikov, K. A., Volozhantsev, N. V., and Knirel, Y. A. (2014) Structure of the capsular polysaccharide of Acinetobacter baumannii ACICU containing di-N-acetylpseudaminic acid, Carbohydr. Res., 391, 89–92.
Kenyon, J. J., Marzaioli, A. M., Hall, R. M., and De Castro, C. (2014) Structure of the K2 capsule associated with the KL2 gene cluster of Acinetobacter baumannii, Glycobiology, 24, 554–563.
Arbatsky, N. P., Shneider, M. M., Shashkov, A. S., Popova, A. V., Miroshnikov, K. A., Volozhantsev, N. V., and Knirel, Y. A. (2016) Structure of the N-acetylpseudaminic acidcontaining capsular polysaccharide of Acinetobacter baumannii NIPH67, Russ. Chem. Bull., 588–591.
Kenyon, J. J., Marzaioli, A. M., Hall, R. M., and De Castro, C. (2015) Structure of the K6 capsular polysaccharide from Acinetobacter baumannii isolate RBH4, Carbohydr. Res., 409, 30–35.
Haseley, S. R., and Wilkinson, S. G. (1996) Structural studies of the putative O-specific polysaccharide of Acinetobacter baumannii O11, Eur. J. Biochem., 237, 266–271.
Kenyon, J. J., Hall, R. M., and De Castro, C. (2015) Structural determination of the K14 capsular polysaccharide from an ST25 Acinetobacter baumannii isolate, D46, Carbohydr. Res., 417, 52–56.
Shashkov, A. S., Kenyon, J. J., Senchenkova, S. N., Shneider, M. M., Popova, A. V., Arbatsky, N. P., Miroshnikov, K. A., Volozhantsev, N. V., Hall, R. M., and Knirel, Y. A. (2016) Acinetobacter baumannii K27 and K44 capsular polysaccharides have the same K unit but different structures due to the presence of distinct wzy genes in otherwise closely related K gene clusters, Glycobiology, 26, 501508.
Lees-Miller, R. G., Iwashkiw, J. A., Scott, N. E., Seper, A., Vinogradov, E., Schild, S., and Feldman, M. F. (2103) A common pathway for O-linked protein-glycosylation and synthesis of capsule in Acinetobacter baumannii, Mol. Microbiol., 89, 816–830.
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Published in Russian in Biokhimiya, 2017, Vol. 82, No. 4, pp. 655-663.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM16-342, December 5, 2016.
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Kasimova, A.A., Shneider, M.M., Arbatsky, N.P. et al. Structure and gene cluster of the K93 capsular polysaccharide of Acinetobacter baumannii B11911 containing 5-N-Acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid. Biochemistry Moscow 82, 483–489 (2017). https://doi.org/10.1134/S0006297917040101
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DOI: https://doi.org/10.1134/S0006297917040101