Abstract
Lymphocyte phosphatase-associated phosphoprotein (LPAP) is a transmembrane protein with unknown function. The available data on its close association with phosphatase CD45 and its phosphorylation depending on cell activation suggest that LPAP can play a significant role in the antigenic stimulation of lymphocytes. We have localized three antigenic epitopes of the LPAP molecule that can be detected using monoclonal antibodies prepared earlier. Experiments on reactions of antibodies with point mutants and shortened forms of the LPAP protein revealed regions of the amino acid sequence that correspond to the epitopes recognized by the antibodies.
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Abbreviations
- BSA:
-
bovine serum albumin
- CD45-AP:
-
CD45-associated protein
- FITC:
-
fluorescein isothiocyanate
- KLH:
-
keyhole limpet hemocyanin
- LPAP:
-
lymphocyte phosphatase-associated phosphoprotein
- PBS:
-
phosphate buffered saline
- PMSF:
-
phenylmethylsulfonyl fluoride
- R6G-SE:
-
rhodamine 6G succinimidyl ester
- SDS:
-
sodium dodecyl sulfate
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Original Russian Text © A. V. Filatov, T. D. Meshkova, D. V. Mazurov, 2014, published in Biokhimiya, 2014, Vol. 79, No. 12, pp. 1705–1714.
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Filatov, A.V., Meshkova, T.D. & Mazurov, D.V. Epitope mapping of lymphocyte phosphatase-associated phosphoprotein. Biochemistry Moscow 79, 1397–1404 (2014). https://doi.org/10.1134/S0006297914120153
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DOI: https://doi.org/10.1134/S0006297914120153