Abstract
The photoreceptor UV RESISTANCE LOCUS 8 (UVR8) activates photomorphogenic responses when plants are exposed to ultraviolet-B (UV-B) light. However, whereas the absorption spectrum of UVR8 peaks at 280 nm, action spectra for several photomorphogenic UV-B responses show maximal photon effectiveness at 290-300 nm. To investigate this apparent discrepancy we measured the effectiveness of UV wavelengths in initiating two responses in Arabidopsis: photoconversion of homodimeric UVR8 into the monomeric form, which is active in signaling, and accumulation of transcripts of the ELONGATED HYPOCOTYL 5 (HY5) transcription factor, which has a key role in UVR8-mediated responses. When purified UVR8 or Arabidopsis leaf extracts were exposed to UV light monomerisation was maximal at approximately 280 nm, which correlates with the UVR8 absorption spectrum. When intact plants were exposed to UV, monomerisation was most strongly initiated at approximately 290 nm, and this shift in maximal effectiveness could be explained by strong absorption or reflectance at 280 nm by leaf tissue. Notably, the action spectrum for accumulation of HY5 transcripts in the same leaf tissue samples used to assay UVR8 dimer/monomer status peaked at approximately 300 nm. Possible reasons for the difference in maximal photon effectiveness of UVR8 monomerisation and HY5 transcript accumulation in leaf tissue are discussed.
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R. L. McKenzie, L. O. Bjorn, A. Bais and M. Ilyasd, Photochem. Photobiol. Sci., 2003, 2, 5–15.
B. R. Jordan, Adv. Bot. Res., 1996, 22, 97–162.
M. A. K. Jansen, Physiol. Plant., 2002, 116, 423–429.
G. I. Jenkins, Annu. Rev. Plant Biol., 2009, 60, 407–431.
T. M. Robson, K. Klem, O. Urban and M. A. K. Jansen, Plant Cell Environ., 2015, 38, 856–866.
P. Casati and V. Walbot, Genome Biol., 2004, 5, R16.
R. Ulm, A. Baumann, A. Oravecz, Z. Mate, E. Adam, E. J. Oakeley, E. Schäfer and F. Nagy, Proc. Natl. Acad. Sci. U. S. A., 2004, 101, 1397–1402.
B. A. Brown, C. Cloix, G. H. Jiang, E. Kaiserli, P. Herzyk, D. J. Kliebenstein and G. I. Jenkins, Proc. Natl. Acad. Sci. U. S. A., 2005, 102, 18225–18230.
J. Kilian, D. Whitehead, J. Horak, D. Wanke, S. Weinl, O. Batistic, C. D'Angelo, E. Bornberg-Bauer, J. Kudla and K. Harter, Plant J., 2007, 50, 347–363.
J. J. Favory, A. Stec, H. Gruber, L. Rizzini, A. Oravecz, M. Funk, A. Albert, C. Cloix, G. I. Jenkins, E. J. Oakeley, H. K. Seidlitz, F. Nagy and R. Ulm, EMBO J., 2009, 28, 591–601.
R. Ulm and F. Nagy, Curr. Opin. Plant Biol., 2005, 8, 477–482.
E. Hideg, M. A. K. Jansen and Ä. Strid, Trends Plant Sci., 2013, 18, 107–115.
G. I. Jenkins, Plant Cell Environ., 2017, 40, 2544–2557.
K. Tilbrook, A. B. Arongaus, M. Binkert, M. Heijde, R. Yin and R. Ulm, The Arabidopsis Book, American Society of Plant Biologists, 2013, p. e0164.
G. I. Jenkins, Plant Cell, 2014, 26, 21–37.
D. J. Kliebenstein, J. E. Lim, L. G. Landry and R. L. Last, Plant Physiol., 2002, 130, 234–243.
A. Coffey, E. Prinsen, M. A. K. Jansen and J. Conway, Plant Cell Environ., 2017, 40, 2250–2260.
L. Rizzini, J.-J. Favory, C. Cloix, D. Faggionato, A. O'Hara, E. Kaiserli, R. Baumeister, E. Schäfer, F. Nagy, G. I. Jenkins and R. Ulm, Science, 2011, 332, 103–106.
J. M. Christie, A. S. Arvai, K. J. Baxter, M. Heilmann, A. J. Pratt, A. O'Hara, S. M. Kelly, M. Hothorn, B. O. Smith, K. Hitomi, G. I. Jenkins and E. D. Getzoff, Science, 2012, 335, 1492–1496.
D. Wu, Q. Hu, Z. Yan, W. Chen, C. Yan, X. Huang, J. Zhang, P. Yang, H. Deng, J. Wang, X. W. Deng and Y. Shi, Nature, 2012, 484, 214–219.
A. O'Hara and G. I. Jenkins, Plant Cell, 2012, 24, 3755–3766.
A. A. Voityuk, R. A. Marcus and M. Michel-Beyerle, Proc. Natl. Acad. Sci. U. S. A., 2014, 111, 5219–5224.
M. Wu, Ä. Strid and L. A. Eriksson, J. Phys. Chem. B, 2014, 118, 951–965.
T. Mathes, M. Heilmann, A. Pandit, J. Zhu, J. Ravensbergen, M. Klos, Y. Fu, B. O. Smith, J. M. Christie, G. I. Jenkins and J. T. M. Kennis, J. Am. Chem. Soc., 2015, 137, 8113–8120.
X. Zeng, Z. Ren, Q. Wu, J. Fan, P. Peng, K. Tang, R. Zhang, K.-H. Zhao and X. Yang, Nat. Plants, 2015, 1, 14006.
X. Huang, X. Ouyang, P. Yang, O. S. Lau, L. Chen, N. Wei and X. W. Deng, Proc. Natl. Acad. Sci. U. S. A., 2013, 110, 16669–16674.
M. Binkert, L. Kozma-Bognâr, K. Terecskei, L. De Veylder, F. Nagy and R. Ulm, Plant Cell, 2014, 26, 4200–4213.
B. A. Brown and G. I. Jenkins, Plant Physiol., 2008, 146, 576–588.
M. Heilmann and G. I. Jenkins, Plant Physiol., 2013, 161, 547–555.
M. Heijde and R. Ulm, Proc. Natl. Acad. Sci. U. S. A., 2013, 110, 1113–1118.
H. Gruber, M. Heijde, W. Heller, A. Albert, H. K. Seidlitz and R. Ulm, Proc. Natl. Acad. Sci. U. S. A., 2010, 107, 20132–20137.
K. M. W. Findlay and G. I. Jenkins, Plant Cell Environ., 2016, 39, 1706–1714.
P. A. Ensminger, Physiol. Plant., 1993, 88, 501–508.
L. Jiang, Y. Wang, L. O. Björn, J.-X. He and S.-S. Li, Plant Signaling Behav., 2012, 7, 1–5.
J. Takeda, R. Nakata, H. Ueno, A. Murakami, M. Iseki and M. Watanabe, Photochem. Photobiol., 2014, 90, 1043–1049.
B. A. Brown, L. R. Headland and G. I. Jenkins, Photochem. Photobiol., 2009, 85, 1147–1155.
E. Kaiserli and G. I. Jenkins, Plant Cell, 2007, 19, 2662–2673.
M. Heilmann, C. N. Velanis, C. Cloix, B. O. Smith, J. M. Christie and G. I. Jenkins, Plant J., 2016, 88, 71–81.
C. Cloix, E. Kaiserli, M. Heilmann, K. J. Baxter, B. A. Brown, A. O'Hara, B. O. Smith, J. M. Christie and G. I. Jenkins, Proc. Natl. Acad. Sci. U. S. A., 2012, 109, 16366–16370.
G. Czégény, M. Wu, A. Dér, L. A. Eriksson, Â. Strid and É. Hideg, FEBS Lett., 2014, 588, 2255–2261.
M. Heilmann, J. M. Christie, J. T. Kennis, G. I. Jenkins and T. Mathes, Photochem. Photobiol. Sci., 2014, 14, 252–257.
T. Miyamori, Y. Nakasone, K. Hitomi, J. M. Christie, E. D. Getzoff and M. Terazima, Photochem. Photobiol. Sci., 2015, 14, 995–1004.
M. Wu, E. Grahn, L. A. Eriksson and Â. Strid, J. Chem. Inf. Model., 2011, 51, 1287–1295.
R. Yin, M. Y. Skvortsova, S. Loubéry and R. Ulm, Proc. Natl. Acad. Sci. U. S. A., 2016, 113, E4415–E4422.
J. Rausenberger, A. Tscheuschler, W. Nordmeier, F. Wüst, J. Timmer, E. Schäfer, C. Fleck and A. Hiltbrunner, Cell, 2011, 146, 813–825.
J. Takeda and S. Abe, Photochem. Photobiol., 1992, 56, 69–74.
J. Takeda, I. Obi and K. Yoshida, Physiol. Plant., 1994, 91, 517–521.
W. Eisinger, T. E. Swartz, R. A. Bogomolni and L. Taiz, Plant Physiol., 2000, 122, 99–105.
K. E. Gerhardt, M. I. Wilson and B. M. Greenberg, Photochem. Photobiol., 2005, 81, 1061–1068.
E. Wellmann, in Encyclopedia of Plant Physiology New Series, ed. W. Shropshire Jr., and H. Mohr, Springer, Berlin, 1983, vol. 16B, pp. 745–756.
G. Gardner, C. Lin, E. M. Tobin, H. Loehrer and D. Brinkman, Plant, Cell Environ., 2009, 32, 1573–1583.
H. Yatsuhashi, T. Hashimoto and S. Shimizu, Plant Physiol., 1982, 70, 735–741.
T. Hashimoto, C. Shichijo and H. Yatsuhashi, J. Photochem. Photobiol., B, 1991, 11, 353–363.
C. J. Beggs and E. Wellmann, Photochem. Photobiol., 1985, 41, 481–486.
Y. L. Ng, K. V. Thimann and S. A. Gordon, Arch. Biochem. Biophys., 1964, 107, 550–558.
I. Kalbina, S. Li, G. Kalbin, L. O. Björn and Â. Strid, Funct. Plant Biol., 2008, 35, 222–227.
M. Loki, S. Takahashi, N. Nakajima, K. Fujikura, M. Tamaoki, H. Saji, A. Kubo, M. Aono, M. Kanna, D. Ogawa, J. Fukazawa, Y. Oda, S. Yoshida, M. Watanabe, S. Hasezawa and N. Kondo, Planta, 2008, 229, 25–36.
Acknowledgements
Aranzazú Díaz-Ramos was supported by a PhD studentship from Consejo Nacional de Ciencia y Tecnología (CONACYT). Andrew O'Hara was supported by a UK Biotechnology and Biological Sciences Research Council PhD studentship (University of Glasgow) and Sven and Lily Lawski's Foundation for Scientific Research (University of Örebro). Selvaraju Kanagarajan was supported by the Carl Trygger Foundation. Daniel Farkas was supported by the Faculty for Business, Science, and Technology at Örebro University. Åke Strid acknowledges financial support for this work from the Knowledge Foundation (kks.se) (20130164), FORMAS - aSwedish Research Council for Sustainable Development (formas.se), and the Faculty for Business, Science, and Technology at Örebro University. We thank the EU COST action FA0906 'UV4Growth' for supporting visits by A. O. to Örebro. We thank Konstancija Vasilenkaite and Monika Heilmann for undertaking preliminary experiments in this project. G. I. J. thanks the University of Glasgow for the support of his research.
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Díaz-Ramos, L.A., O'Hara, A., Kanagarajan, S. et al. Difference in the action spectra for UVR8 monomerisation and HY5 transcript accumulation in Arabidopsis. Photochem Photobiol Sci 17, 1108–1117 (2018). https://doi.org/10.1039/c8pp00138c
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DOI: https://doi.org/10.1039/c8pp00138c