Abstract
The mammalian epidermis has evolved to protect the body in a dry environment. Genes of the epidermal differentiation complex (EDC), such as FLG (filaggrin), are implicated in the barrier function of the epidermis. Here, we investigated the molecular evolution of the EDC in sirenians (manatees and dugong), which have adapted to fully aquatic life, in comparison to the EDC of terrestrial mammals and aquatic mammals of the clade Cetacea (whales and dolphins). We show that the main subtypes of EDC genes are conserved or even duplicated, like late cornified envelope (LCE) genes of the dugong, whereas specific EDC genes have undergone inactivating mutations in sirenians. FLG contains premature stop codons in the dugong, and the ortholog of human CASP14 (caspase-14), which proteolytically processes filaggrin, is pseudogenized in the same species. As FLG and CASP14 have also been lost in whales, these mutations represent convergent evolution of skin barrier genes in different lineages of aquatic mammals. In contrast to the dugong, the manatee has retained functional FLG and CASP14 genes. FLG2 (filaggrin 2) is truncated in both species of sirenians investigated. We conclude that the land-to-water transition of sirenians was associated with modifications of the epidermal barrier at the molecular level.
Similar content being viewed by others
Introduction
Life on land depends on the protection against excessive loss of water from the body in a dry environment1,2. The control of water loss is mediated in part by the outermost, epithelial compartment of the skin, the epidermis. Within this stratified epithelium, keratinocytes proliferate in the basal layer and differentiate during their movement towards the skin surface while passing through the suprabasal layers (Fig. 1). Keratinocyte differentiation involves the accumulation of cytoskeletal proteins and enzymes that are required for establishing the barrier against the environment which mainly resides in the granular layer and the cornified layer of the epidermis. The transition of keratinocytes from the granular to the cornified layer is associated with programmed cell death and cross-linking of proteins to form a mechanically and chemically resilient protein envelope to which lipids are attached3,4,5,6. The integrity of cornified cell envelopes is essential for the barrier function of the epidermis7,8.
A cluster of genes encoding protein components of the cornified envelope of epidermal keratinocytes is known as the epidermal differentiation complex (EDC). The EDC is located on human chromosome 1q21.3, and homologous gene clusters have been identified in other mammalian species9,10,11,12 as well as non-mammalian tetrapods13,14,15,16,17,18. The EDC is bordered by genes of the S100A family, which are considered the evolutionarily ancestors of other EDC gene types13,19. Genes in the core region of the EDC can be classified into peptidoglycan recognition protein genes, single-coding-exon EDC (SEDC) genes and S100 fused-type protein (SFTP) genes. Peptidoglycan recognition protein 3 (PGLYRP3) and PGLYRP4 genes have antimicrobial functions20 and differ substantially from other EDC genes13. SEDCs contain one protein-coding exon and one non-coding exon, whereas SFTPs comprise one non-coding and two protein-coding exons13. Gene families, such as small proline-rich proteins (SPRRs) and late-cornified envelope (LCE) genes, are crucial for the formation of the cornified envelope and belong to the SEDCs. Loricrin, involucrin, keratinocyte proline rich protein (KPRP), KPRP N-terminal and LCE C-terminal like protein (KPLCE), which was formerly known as LEP7, XP32 or C1orf68 (chromosome 1 open reading frame 68), proline rich 9 (PRR9) and late cornified envelope like proline rich 1 (LELP1) are SEDCs that exist as single-copy genes in the human genome.
SFTPs contain an S100 domain at their N-terminus and a long, sequence repeat-rich domain at the C-terminus9,10. Additionally, a short C-terminal sequence motif is conserved in most SFTPs21. Seven SFTP genes, i.e. cornulin (CRNN), filaggrin (FLG), filaggrin 2 (FLG2), hornerin (HRNR), repetin (RPTN), trichohyalin (TCHH) and trichohyalin-like1 (TCHHL1), are present in the human EDC. The best characterized genes among the latter are FLG and TCHH. FLG contributes to keratin filament aggregation in the epidermis, hydration of the stratum corneum and UV protection of the skin22. On histological sections, FLG forms, together with other proteins, basophilic keratohyalin granules in late differentiated but not yet cornified keratinocytes which form the granular layer of the epidermis. Mutations of the FLG gene are linked to skin barrier diseases, such as ichthyosis vulgaris and atopic dermatitis23. TCHH interacts with keratins and is expressed in the inner root sheath of the hair follicle, the tongue filiform papillae and the nail isthmus24.
Two clades of mammals have adapted to a fully aquatic lifestyle, cetaceans and sirenians. The former comprise whales, dolphins and porpoises and, together with artiodactyls, form the clade Cetartiodactyla within the superorder Laurasiatheria. Sirenians comprise manatees and dugongs and belong to the superorder of Afrotheria, with proboscideans (elephants) being their closest extant relatives25. Land-dwelling ancestors of cetaceans and sirenians independently underwent the evolutionary transition to life in the sea.
The skin of sirenians differs histologically from that of terrestrial mammals and shows some similarities to that of cetaceans, as it contains a subcutaneous fat layer called blubber and lacks sweat glands, and the epidermis is thicker than that of terrestrial mammals26,27 (Fig. 1). Furthermore, the epidermis lacks a granular layer and contains a thickened cornified layer of incompletely characterized structure in sirenians and cetaceans27,28,29. Specialized epithelial structures, namely vibrissae and keratinized pads that replace incisors have evolved as an adaptation of sirenians to feeding on seagrass30.
The genes encoding many epidermal proteins have been studied in detail in cetaceans, but only very incompletely in sirenians. Among EDC genes, LOR, IVL, SPRRs and CRCT1 have been conserved in cetaceans, whereas KPRP, KPLCE and LCEs with the exception of LCE7A are absent in all cetaceans and PRR9 and LELP1 have been lost in subclades of cetaceans11. Keratins forming the cytoskeleton in the suprabasal epidermis of land-dwelling mammals, i.e. KRT1, KRT2, KRT9 and KRT10, are not conserved in cetaceans and they are also inactivated by mutations in the manatee31,32. Additional genes with functions in the epidermis were lost in cetaceans33,34.
In the present study, we analyzed the EDC of two species of sirenians in comparison to their homologs in humans and other mammals. We report that the coding sequence of the important skin barrier gene FLG is truncated and the FLG-processing protease, caspase-14, is inactivated by mutations in the dugong. However, we also demonstrate that most other EDC genes are conserved in sirenians and encode functional proteins, indicating roles of EDC genes that are not associated with the barrier to a dry environment.
Results
Identification of the EDC in the genomes of sirenians
We investigated the EDC in the partly annotated genome sequence of the manatee and the not-yet-annotated genome sequence assembly of the dugong (Supplementary Tables S1, S2; Supplementary Figs. S1, S2). The gene organization of the EDC of sirenians was compared to the EDC in the Asian elephant (Elephas maximus indicus) (Supplementary Table S3; Supplementary Fig. S3), as a representative member of the phylogenetically closest terrestrial clade of mammals, the order Proboscidea. Furthermore, the human EDC was included in comparative analyses. The sequence of the EDC of the dugong was available as a continuous scaffold without sequence gaps, whereas genes of EDC of the manatee were identified on different sequence contigs that were not finally assembled at the time of this study (December 2023) (Fig. 2).
The EDC of both species of sirenians is comprised of S100A, PGLYRP, SEDC and SFTP genes in an arrangement homologous to that in other mammals9,10,11. We focused on the genes located between S100A9 and S100A11. PGLYRP3 is free of disruptive mutations, whereas PGLYRP4 contains inactivating mutations in its coding sequence (Supplementary Fig. S4). Conservation of PGLYRP3 and loss of functional PGLYRP4 was also detected in the elephant, suggesting that the inactivation of PGLYRP4 has occurred in a common ancestor of sirenians and elephants. Both intact genes and pseudogenes were also identified among the main types of EDC genes, that is, SEDCs and SFTPs, as will be described in detail below.
Late cornified envelope (LCE) genes have been amplified in the dugong
Comparative analysis showed that sirenians have orthologs of all subtypes of SEDC genes (Fig. 2). Loricrin, PRR9, LELP1, involucrin (IVL), SMCP, KPRP, KPLCE and CRCT1 are present as single copy genes in both manatee and dugong (Fig. 2, Supplementary Tables S1 and S2). Multiple paralogs of SPRRs and LCE genes are arranged in gene clusters in sirenians, similar to their homologs in elephants and humans. Due to gaps in the genome sequence of the manatee, the precise arrangement and the numbers of SPRR and LCE genes could not be determined for the manatee. In the dugong, twenty-one protein-coding SPRR genes and additional pseudogenized SPRRs are located between the LELP1 and IVL genes. This number of SPRRs is smaller than that in the elephant (n = 34), but larger than the number of human SPRR genes (n = 12).
Strikingly, the number of LCE genes is greatly increased in the dugong as compared to both elephant and humans. With 3 LCE genes in cluster 1 between SMCP and KPRP and 47 LCE genes in cluster 2 between KPLCE and CRCT1, the dugong has more than twice as many LCE genes as humans (n = 19) and the elephant (n = 15) (Figs. 2 and 3). The increase in the number of LCEs is due to the amplification of LCE2 paralogs which show slight variation at amino acid positions of the entire length of the protein (Fig. 3). Phylogenetic analysis confirmed that the main cluster of LCE genes of the dugong is monophyletic (Supplementary Fig. S5). Fewer LCE paralogs were identified in the genome of the manatee, which, however, contained several gaps in the region of the LCE genes (Fig. 2).
Divergent evolution of KPLCE in elephants and sirenians
KPLCE is a gene that has been recently re-named by GenBank after it was originally reported as LEP7, XP32 or C1orf68. The KPLCE protein is characterized by a tripartite organization with an N-terminal segment, a central region with imperfect sequence repeats (Supplementary Fig. S6) and a C-terminal segment, which are largely conserved across species (Fig. 4). However, KPLCE of the manatee has an unusual organization as it contains more sequence repeats than its homologs in other species and lacks the C-terminal segment (Fig. 4). The EDC of the Asian elephant contains 7 copies of KPLCE, of which 6 encode proteins and one is a pseudogene (Fig. 2). The KPLCE proteins of the elephant are characterized by a shortened C-terminal segment, which lacks a subsegment of 59 amino acid residues present in human KPLCE (Fig. 4).
To estimate when in evolution the copies of KPLCE have emerged, we investigated the EDC of the African Savannah elephant (Loxodonta africana) and the rock hyrax (Procavia capensis). The African elephant has at least two intact copies of KPLCE, whereas the hyrax has only one (Fig. 4). This pattern suggests that the amplification of KPLCE has occurred in the phylogenetic lineage leading to elephants, and only one KPLCE gene was present in the common ancestor of sirenians, elephants and hyrax.
Filaggrin and trichohyalin-like 1 genes contain premature stop codons in sirenians
SFTP genes form a cluster in the EDC of sirenians like in other mammals. All of the SFTP genes present in humans and elephants have homologs in sirenians (Fig. 2). However, due to premature stop codons the proteins encoded by FLG, FLG2 and TCHHL1 are more than 50% shorter in sirenians than in elephants and humans (Fig. 5A, Supplementary Fig. S7). A characteristic short amino sequence motif, that has been suggested to mediate binding of SFTPs to keratins35, is conserved in 6 out of 7 SFTPs of humans and elephants (Fig. 5B), but only in 4 and 5 proteins encoded by SFTP genes of the dugong and manatee, respectively. Both species of sirenians lack the C-terminal motif in the predicted FLG2 and TCHHL1 proteins (Fig. 5B). The C-terminal motif of SFTPs21 is present in FLG of the manatee but absent in FLG of the dugong. FLG2 of the dugong is predicted to be extremely short because of an in-frame stop codon in the currently available genome sequence. The sequence downstream of this predicted stop codon does not contain further stops for more than 2000 codons, suggesting that this gene has acquired the premature stop only recently in evolution.
SFTPs of sirenians and other species contain an N-terminal S100 domain of around 90 amino acid residues, followed by a long highly repetitive sequence that is strongly biased to only few amino acid residues. This leads to an extreme enrichment of few amino acids in many SFTPs. In line with this notion, only two amino acids, i.e. arginine (R) and glutamic acid (E), account for approximately 50% of all residues of TCHH in sirenians, strongly resembling TCHH in elephant and humans (Fig. 5C). Likewise, the high glycine and serine contents are conserved in HRNR of sirenians (Fig. 5C). Overall, the SFTPs of sirenians have a similar amino acid composition as their homologs in terrestrial mammals.
Caspase-14 is inactivated by mutations in the dugong
As FLG is an important skin barrier protein and mutations of the human FLG gene are associated with ichthyosis vulgaris and atopic dermatitis36,37, we investigated FLG-interacting proteins in the manatee, which has retained FLG, and the dugong, which has lost the C-terminal portion of FLG (Fig. 5A,B). Two proteases, aspartic peptidase retroviral like 1 (ASPRV1) and caspase-14 (CASP14), are expressed specifically in terminally differentiated keratinocytes where they are involved in the proteolytic processing of filaggrin38,39. ASPRV1 is conserved in the manatee and the dugong (Supplementary Fig. S8). By contrast, the CASP14 gene is conserved only in the manatee, whereas it is disrupted by a premature stop codon and a frameshift mutation in the dugong (Fig. 6). All disruptive mutations of CASP14 were present in three dugong genome sequences that were available in GenBank as results of independent projects (Supplementary Fig. S9).
Discussion
The main function of keratinocyte differentiation is the establishment of the body’s interface with the environment3,40. Accordingly, adaptations to different environments are expected to involve adaptations of keratinocyte differentiation. Our results support this hypothesis with regard to mutations of genes, such as FLG and CASP14, implicated in the epidermal barrier formation in land-dwelling mammals. However, the extent of gene loss in the keratinocyte differentiation program is less pronounced than that in the other major group of aquatic mammals, the cetaceans11,41,42 (Fig. 7).
Sirenians have apparently intact KPRP, KPLCE, PRR9, LELP1 and LCEs, the orthologs of which have been lost in cetaceans11. Our analysis shows that LCE genes are even amplified in the dugong, whereas the incompleteness of the current genome sequence assembly of the manatee does not allow to conclude on the number of LCE genes in this species. The increase of LCE gene copy numbers in the dugong has likely occurred through gene duplications by the mechanism of unequal crossing over43. The retention of the duplicated genes suggests that they have provided a selective advantage, for example by increasing the dosage of the encoded proteins or by facilitating subfunctionalization44. However, the possibility of neutral evolution of gene copy numbers needs to be considered45, and even potentially deleterious effects of large tandemly arrayed gene clusters have been discussed46. In humans, LCE proteins are components of cornified envelopes47. Their expression is increased upon exposure of the skin to ultraviolet radiation48 and during the repair of the skin barrier49, whereas lack of LCE3B and LCE3C due to gene loss predisposes to psoriasis50. LCEs have antimicrobial activities51 and interact with the antimicrobial cysteine-rich tail protein 1 (CYSRT1)50. It remains to be investigated which function of LCEs has been retained in sirenians whereas it is dispensable in cetaceans. Another antimicrobial protein encoded by an EDC gene, PGLYRP4, is absent in both sirenians and cetaceans11, indicating that this protein is dispensable for fully aquatic mammals.
In contrast to SEDC genes, the SFTP gene clusters of sirenians are affected by several mutations which are predicted to impair the normal function of the encoded proteins. The proteins encoded by the genes FLG, FLG2 and TCHHL1 are much shorter in sirenians than their orthologs in other mammalian species. Interestingly, the manatee has a potentially functional FLG including the characteristic C-terminal sequence motif of SFTPs (Fig. 5B), whereas FLG of the dugong is truncated and lacks this motif. Human FLG is probably the most-investigated EDC gene because polymorphisms of FLG affect skin barrier properties21,52 and FLG mutations are associated with the highly prevalent inflammatory skin disease, atopic dermatitis37. Both FLG2 and TCHHL1 are truncated by premature stop codons in sirenians (Fig. 5A,B). FLG2 is a component of cornified envelopes53 and mutations of the FLG2 gene cause peeling skin syndrome type A54. TCHHL1 is expressed in hair follicles55, and TCHHL1 protein was detected by mass spectrometry-based proteomics in mature hair shafts of mice56. As sirenians have a few hairs with putative mechanosensory functions, hair-related genes are not generally lost31. Accordingly, the main SFTP of the inner root sheath of hair follicles, TCHH, is conserved in both manatee and dugong. The comparison of SFTP genes in cetaceans41 and sirenians (this study) reveals striking differences, because all SFTPs have been lost in whales and only FLG is conserved in dolphins, whereas many SFTPs are conserved in sirenians.
Our finding of parallel loss of FLG and CASP14 in the dugong suggests that a common pathway involving both proteins has been lost in the dugong. Caspase-14 is co-expressed with FLG57 and proteolytically processes FLG in murine and human keratinocytes39,58. However, FLG and CASP14 have not been strictly interdependent during the evolution of mammals. CASP14 is present in monotremes (platypus and echidna), whereas an SFTP with amino acid sequence features characteristic for FLG is missing12. CASP14 has been lost in cetaceans, whereas FLG has been conserved, as mention above, in a subgroup of cetaceans41. Deletions in the human CASP14 gene have been linked to a defect in cornification that manifests as autosomal recessive inherited ichthyosis59. The cellular features of the epidermis in manatees, which have FLG and CASP14, and dugongs, which lack FLG and CASP14, remain to be investigated in future studies.
Although the availability of genome sequences has provided insights into changes of keratinocyte differentiation genes, it is important to notice the limitations of the present study. First, the expression of EDC genes of sirenians remains to be investigated in situ, that is, in skin samples of manatees and dugongs. As protein sequences can be faithfully predicted now, proteomic analysis appears to be straightforward. Second, keratinocyte differentiation could not be studied in an in vitro model, because the culture of skin cells of sirenians is only in its infancy60,61, and fresh biosamples were not available to us. Finally, the interpretation of sequence data must be done cautiously because errors of DNA-sequencing and sequence assembly cannot be excluded.
Material and methods
Ethics statement
Genome and transcriptome data were obtained from public databases. This study involved neither humans nor animals.
Identification of EDC genes in genomic sequences
Homologs of human EDC genes were identified by searches with the basic local alignment search tool (BLAST) at the NCBI website (https://blast.ncbi.nlm.nih.gov/Blast.cgi, last accessed on 21 December 2023) and analysis of the genomic region between the genes S100A9 and S100A11 in the genomes of the dugong (Dugong dugon, mDugDug1.hap1, GenBank accession number GCA_030035585.1, submitted by Vertebrate Genomes Project)62, manatee (Trichechus manatus latirostris, GenBank accession number GCA_030013775.1, submitted by Consejo Superior de Investigaciones Cientificas, Valencia, Spain) and elephant (Elephas maximus indicus, GenBank accession number GCF_024166365.1, submitted by Vertebrate Genomes Project). Sequences of the dugong that were considered important for the conclusions of this study, were analyzed in two additional dugong genome sequences (Dugong dugon assembly, WGS project CAJQER01, GenBank accession number GCA_905400935.1, submitted by Max-Planck Institute for Evolutionary Anthropology, Leipzig, Germany; Dugong dugon genome assembly D_dugong, WGS project BMBL01, GenBank accession number GCA_015147995.1, submitted by National Institute for Environmental Studies, Japan). The EDC region around KPLCE was analyzed in the genome sequence of another species of Proboscidea, the African savannah elephant (Loxodonta africana, GenBank accession number GCA_030014295.1, submitted by Vertebrate Genomes Project), and the hyrax (Procavia capensis, GenBank accession number GCA_000152225.2, submitted by Baylor College of Medicine, Houston, Texas). For some EDC genes, annotations were available in the genome sequence assemblies of NCBI GenBank, as indicated in Supplementary Tables S1–S3. Other EDC genes were identified by tBLASTn searches using proteins encoded in the EDC of humans or Afrotherian species as queries. To avoid false elimination of hits with biased amino acid composition characteristic for EDC proteins13,24, the filter for low sequence complexity was deactivated. Criteria for gene orthology were shared local synteny and reciprocal best hits in BLAST searches63.
Analysis of amino acid sequences encoded by EDC genes
Amino acid sequences were aligned with MUSCLE64 and MultAlin65. The alignments were manually adjusted. Amino acid contents of proteins were calculated with the ProtParam tool at the ExPASy portal66. For the visualization of sequence repeats in KPLCE proteins, sequence logos were generated using the Weblogo software67.
Molecular phylogenetics
Sequences belonging to the LCE family were collected from NCBI GenBank for each species of interest. The phylogenetic analysis was performed with PhyML (version 3.3.20220408)68 according to an approach described previously19. Phylogenetic trees were visualized and edited with FigTree (http://tree.bio.ed.ac.uk/software/figtree/, last accessed on December 17, 2023) and inkscape (version: 1.0.0.0; https://inkscape.org/de/, accessed on December 17, 2023).
Data availability
All data generated or analyzed during this study are included in this published article and its Supplementary Information files.
References
Alibardi, L. Adaptation to the land: The skin of reptiles in comparison to that of amphibians and endotherm amniotes. J. Exp. Zool. B Mol. Dev. Evol. 298, 12–41 (2003).
Matsui, T. & Amagai, M. Dissecting the formation, structure and barrier function of the stratum corneum. Int. Immunol. 27, 269–280 (2015).
Watt, F. M. Terminal differentiation of epidermal keratinocytes. Curr. Opin. Cell Biol. 1, 1107–1115 (1989).
Candi, E., Schmidt, R. & Melino, G. The cornified envelope: A model of cell death in the skin. Nat. Rev. Mol. Cell Biol. 6, 328–340 (2005).
Sachslehner, A. P. et al. Transglutaminase activity is conserved in stratified epithelia and skin appendages of mammals and birds. Int. J. Mol. Sci. 24, 2193 (2023).
Crumrine, D. et al. Mutations in recessive congenital ichthyoses illuminate the origin and functions of the corneocyte lipid envelope. J. Investig. Dermatol. 139, 760–768 (2019).
Matsuki, M. et al. Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1 (keratinocyte transglutaminase). Proc. Natl. Acad. Sci. U. S. A. 95, 1044–1049 (1998).
Jonca, N. & Simon, M. The cornified envelope: A versatile contributor to the epidermal barrier. J. Investig. Dermatol. 143, 1335–1337 (2023).
Henry, J. et al. Update on the epidermal differentiation complex. Front. Biosci. 17, 1517–1532 (2012).
Kypriotou, M., Huber, M. & Hohl, D. The human epidermal differentiation complex, cornified envelope precursors, S100 proteins and the “fused genes” family. Exp. Dermatol. 21, 643–649 (2012).
Holthaus, K. B., Lachner, J., Ebner, B., Tschachler, E. & Eckhart, L. Gene duplications and gene loss in the epidermal differentiation complex during the evolutionary land-to-water transition of cetaceans. Sci. Rep. 11, 12334 (2021).
Steinbinder, J., Sachslehner, A. P., Holthaus, K. B. & Eckhart, L. Comparative genomics of monotremes provides insights into the early evolution of mammalian epidermal differentiation genes. Sci. Rep. 14, 1437 (2024).
Strasser, B. et al. Evolutionary origin and diversification of epidermal barrier proteins in amniotes. Mol. Biol. Evol. 31, 3194–3205 (2014).
Holthaus, K. B. et al. Comparative genomics identifies epidermal proteins associated with the evolution of the turtle shell. Mol. Biol. Evol. 33, 726–737 (2016).
Holthaus, K. B. et al. Identification and comparative analysis of the epidermal differentiation complex in snakes. Sci. Rep. 7, 45338 (2017).
Holthaus, K. B. et al. Comparative analysis of epidermal differentiation genes of crocodilians suggests new models for the evolutionary origin of avian feather proteins. Genome Biol. Evol. 10, 694–704 (2018).
Holthaus, K. B., Alibardi, L., Tschachler, E. & Eckhart, L. Identification of epidermal differentiation genes of the tuatara provides insights into the early evolution of lepidosaurian skin. Sci. Rep. 10, 12844 (2020).
Davis, A. & Greenwold, M. J. Evolution of an epidermal differentiation complex (EDC) gene family in birds. Genes (Basel) 12, 767 (2021).
Sachslehner, A. P. & Eckhart, L. Evolutionary diversification of epidermal barrier genes in amphibians. Sci. Rep. 12, 13634 (2022).
Kashyap, D. R. et al. Peptidoglycan recognition proteins kill bacteria by activating protein-sensing two-component systems. Nat. Med. 17, 676–683 (2011).
Mlitz, V., Hussain, T., Tschachler, E. & Eckhart, L. Filaggrin has evolved from an “S100 fused-type protein” (SFTP) gene present in a common ancestor of amphibians and mammals. Exp. Dermatol. 26, 955–957 (2017).
Kezic, S. & Jakasa, I. Filaggrin and skin barrier function. Curr. Probl. Dermatol. 49, 1–7 (2016).
McLean, W. H. Filaggrin failure—From ichthyosis vulgaris to atopic eczema and beyond. Br. J. Dermatol. 175, 4–7 (2016).
Mlitz, V. et al. Trichohyalin-like proteins have evolutionarily conserved roles in the morphogenesis of skin appendages. J. Investig. Dermatol. 134, 2685–2692 (2014).
Heritage, S. & Seiffert, E. R. Total evidence time-scaled phylogenetic and biogeographic models for the evolution of sea cows (Sirenia, Afrotheria). PeerJ 10, e13886 (2022).
Horgan, P. et al. Insulative capacity of the integument of the dugong (Dugong dugon): Thermal conductivity, conductance and resistance measured by in vitro heat flux. Mar. Biol. 161, 1395–1407 (2014).
Graham, A. Histological examination of the Florida manatee (Trichechus manatus latirostris) integument. Dissertation, The University of Florida, pp 175. http://ufdcimages.uflib.ufl.edu/UF/E0/01/33/43/00001/graham_a.pdf (2005).
Menon, G. K., Elias, P. M., Wakefield, J. S. & Crumrine, D. Cetacean epidermal specialization: A review. Anat. Histol. Embryol. 51, 563–575 (2022).
Elias, P. M., Menon, G. K., Grayson, S., Brown, B. E. & Rehfeld, S. J. Avian sebokeratocytes and marine mammal lipokeratinocytes: Structural, lipid biochemical, and functional considerations. Am. J. Anat. 180, 161–177 (1987).
Hautier, L. et al. From teeth to pad: Tooth loss and development of keratinous structures in sirenians. Proc. Biol. Sci. 290, 20231932 (2023).
Ehrlich, F. et al. Differential evolution of the epidermal keratin cytoskeleton in terrestrial and aquatic mammals. Mol. Biol. Evol. 36, 328–340 (2019).
Eckhart, L., Ehrlich, F. & Tschachler, E. A stress response program at the origin of evolutionary innovation in the skin. Evol. Bioinform. Online 15, 1176934319862246 (2019).
Huelsmann, M. et al. Genes lost during the transition from land to water in cetaceans highlight genomic changes associated with aquatic adaptations. Sci. Adv. 5, eaaw6671 (2019).
Zhang, X. et al. Parallel independent losses of G-type lysozyme genes in hairless aquatic mammals. Genome Biol. Evol. 13, evab201 (2021).
Takase, T. & Hirai, Y. Identification of the C-terminal tail domain of AHF/trichohyalin as the critical site for modulation of the keratin filamentous meshwork in the keratinocyte. J. Dermatol. Sci. 65, 141–148 (2012).
Smith, F. J. et al. Loss-of-function mutations in the gene encoding filaggrin cause ichthyosis vulgaris. Nat. Genet. 38, 337–342 (2006).
Palmer, C. N. et al. Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis. Nat. Genet. 38, 441–446 (2006).
Matsui, T. et al. SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing. EMBO Mol. Med. 3, 320–333 (2011).
Hoste, E. et al. Caspase-14 is required for filaggrin degradation to natural moisturizing factors in the skin. J. Investig. Dermatol. 131, 2233–2241 (2011).
Eckhart, L. & Zeeuwen, P. L. J. M. The skin barrier: Epidermis vs environment. Exp. Dermatol. 27, 805–806 (2018).
Strasser, B., Mlitz, V., Fischer, H., Tschachler, E. & Eckhart, L. Comparative genomics reveals conservation of filaggrin and loss of caspase-14 in dolphins. Exp. Dermatol. 24, 365–369 (2015).
Espregueira, T. G. et al. Losing genes: The evolutionary remodeling of cetacea skin. Front. Mar. Sci. 7, 592375 (2020).
Reams, A. B. & Rothm, J. R. Mechanisms of gene duplication and amplification. Cold Spring Harb. Perspect. Biol. 7, a016592 (2015).
Kuzmin, E., Taylor, J. S. & Boone, C. Retention of duplicated genes in evolution. Trends Genet. 38, 59–72 (2022).
Wagner, A. Decoupled evolution of coding region and mRNA expression patterns after gene duplication: Implications for the neutralist-selectionist debate. Proc. Natl. Acad. Sci. U. S. A. 97, 6579–6584 (2000).
Schiffer, P. H., Gravemeyer, J., Rauscher, M. & Wiehe, T. Ultra large gene families: A matter of adaptation or genomic parasites?. Life (Basel) 6, 32 (2016).
Ishitsuka, Y. et al. Lce1 family members are Nrf2-target genes that are induced to compensate for the loss of loricrin. J. Investig. Dermatol. 136, 1656–1663 (2016).
Jackson, B. et al. Late cornified envelope family in differentiating epithelia–response to calcium and ultraviolet irradiation. J. Investig. Dermatol. 124, 1062–1070 (2005).
de Koning, H. D. et al. Expression profile of cornified envelope structural proteins and keratinocyte differentiation-regulating proteins during skin barrier repair. Br. J. Dermatol. 166, 1245–1254 (2012).
Niehues, H. et al. CYSRT1: An antimicrobial epidermal protein that can interact with late cornified envelope proteins. J. Investig. Dermatol. 143, 1498–1508 (2023).
Niehues, H. et al. Antimicrobial late cornified envelope proteins: The psoriasis risk factor deletion of LCE3B/C genes affects microbiota composition. J. Investig. Dermatol. 142, 1947–1955 (2022).
Brown, S. J. et al. Intragenic copy number variation within filaggrin contributes to the risk of atopic dermatitis with a dose-dependent effect. J. Investig. Dermatol. 132, 98–104 (2012).
Albérola, G., Schröder, J. M., Froment, C. & Simon, M. The amino-terminal part of human FLG2 is a component of cornified envelopes. J. Investig. Dermatol. 139, 1395–1397 (2019).
Mohamad, J. et al. Filaggrin 2 deficiency results in abnormal cell-cell adhesion in the cornified cell layers and causes peeling skin syndrome type A. J. Investig. Dermatol. 138, 1736–1743 (2018).
Wu, Z., Latendorf, T., Meyer-Hoffert, U. & Schröder, J. M. Identification of trichohyalin-like 1, an S100 fused-type protein selectively expressed in hair follicles. J. Investig. Dermatol. 131, 1761–1763 (2011).
Sukseree, S. et al. Autophagy controls the protein composition of hair shafts. J. Investig. Dermatol. 144, 170–173 (2024).
Fischer, H. et al. Caspase-14 but not caspase-3 is processed during the development of fetal mouse epidermis. Differentiation 73, 406–413 (2005).
Denecker, G. et al. Caspase-14 protects against epidermal UVB photodamage and water loss. Nat. Cell Biol. 9, 666–674 (2007).
Kirchmeier, P., Zimmer, A., Bouadjar, B., Rösler, B. & Fischer, J. Whole-exome-sequencing reveals small deletions in CASP14 in patients with autosomal recessive inherited ichthyosis. Acta Derm. Venereol. 97, 102–104 (2017).
Nascimento, M. B. et al. The initial steps toward the formation of somatic tissue banks and cell cultures derived from captive Antillean manatee (Trichechus manatus manatus) skin biopsies. Zoo Biol. 42, 709–722 (2023).
Tavares, F. D. S. et al. Establishment and characterization of a primary fibroblast cell culture from the Amazonian manatee (Trichechus inunguis). Animals 14, 686 (2024).
Baker, D. N. et al. A chromosome-level genome assembly for the dugong (Dugong dugon). J. Hered. 115, 212–220 (2024).
Moreno-Hagelsieb, G. & Latimer, K. Choosing BLAST options for better detection of orthologs as reciprocal best hits. Bioinformatics 24, 319–324 (2008).
Edgar, R. C. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32, 1792–1797 (2004).
Corpet, F. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 16, 10881–10890 (1988).
Artimo, P. et al. ExPASy: SIB bioinformatics resource portal. Nucleic Acids Res. 40, 597–603 (2012).
Crooks, G. E., Hon, G., Chandonia, J. M. & Brenner, S. E. WebLogo: A sequence logo generator. Genome Res. 14, 1188–1190 (2004).
Guindon, S. & Gascuel, O. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biol. 52, 696–704 (2003).
Acknowledgements
We thank Bettina Ebner, Julia Lachner, Veronika Mlitz and Florian Ehrlich for helpful discussions during early stages of the project. This work was supported by the Austrian Science Fund (FWF): P32777.
Funding
The funding was supported by Austrian Science Fund, https://doi.org/10.55776/P32777.
Author information
Authors and Affiliations
Contributions
J.S., A.P.S., K.B.H, and L.E. conceived the study, J.S., and A.P.S. performed phylogenetic analyses, J.S., A.P.S., K.B.H. and L.E. analyzed genome and transcriptome sequences, J.S., A.P.S., K.B.H. and L.E. wrote the manuscript.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Steinbinder, J., Sachslehner, A.P., Holthaus, K.B. et al. Comparative genomics of sirenians reveals evolution of filaggrin and caspase-14 upon adaptation of the epidermis to aquatic life. Sci Rep 14, 9278 (2024). https://doi.org/10.1038/s41598-024-60099-2
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-024-60099-2
- Springer Nature Limited