Abstract
Increasing demand for green or biological nanoparticles has led to various green technologies and resources, which play a critical role in forming biocompatible or green nanoparticles. So far, numerous medicinal plants have been explored for this purpose, assuming that medicinal components from the plant's material will contribute to corona formation around nanoparticles and enhance their efficacy. Research is also extended to other green and waste resources to be utilized for this purpose. In the current study, we explored Ligustrum vulgare berries, also known as privet berries, to reduce gold and silver salts into nanoparticles. L. vulgare berries showed great potential to form these nanoparticles, as gold nanoparticles (LV-AuNPs) formed within 5 min at room temperature, and silver nanoparticles (LV-AgNPs) formed in 15 min at 90 °C. LV-AuNPs and LV-AgNPs were characterized by various analytical methods, including UV–Vis, SEM, EDX, TEM, DLS, sp-ICP-MS, TGA, FT-IR, and MALDI-TOF. The results demonstrate that the LV-AuNPs are polydisperse in appearance with a size range 50–200 nm. LV-AuNPs exhibit various shapes, including spherical, triangular, hexagonal, rod, cuboid, etc. In contrast, LV-AgNPs are quite monodisperse, 20–70 nm, and most of the population was spherical. The nanoparticles remain stable over long periods and exhibit high negative zeta potential values. The antimicrobial investigation of LV-AgNPs demonstrated that the nanoparticles exhibit antibacterial ability with an MBC value of 150 g/mL against P. aeruginosa and 100 g/mL against E. coli, as determined by plate assay, live and dead staining, and SEM cell morphology analysis.
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Introduction
The global search for suitable methods for the generation of green nanoparticles leads to the rapid development of new technologies and discovery of new resources for nanoparticles synthesis. Especially, there is a high demand for green methods that can produce nanoparticles in an eco-friendly manner and maintain long-term stability. Green synthesis is also catching attention because of the corona layer formation surrounding the nanoparticles. In green synthesis, the source (biomaterial) constituents help reduce metal salts to nanoparticles and play a major role in stabilizing nanoparticles by forming a capping layer that surrounds the nanoparticles called the “biological corona”. In addition to providing stability, the biological corona contributes to nanoparticles' biocompatibility and affects their mode of action1.
The simplest way to achieve green nanoparticles is to use biological resources, such as microorganisms, plants, or their parts, for nanoparticle production. So far, numerous microorganisms have been explored for this purpose and found efficient in green nanoparticles synthesis, with synthesis occurring either extra or intracellularly, with monodispersity and long-term stability of nanoparticles2,3. However, there are certain limitations with microbial production, such as the demand for specific growth conditions, culture incubators that use energy resources, complex purification systems that require several cycles of high-speed centrifugation, washing, and sometimes ultrasonication to break the membrane for collecting intracellular nanoparticles4. These limitations can be overcome in green synthesis by utilizing plants. Various plants have been found suitable for the rapid production of facile, eco-friendly nanoparticles without energy consumption. Interestingly, plant-based nanoparticle production typically happens within a few seconds to minutes. In addition, plant-derived nanoparticles are quite stable, biocompatible, and monodisperse5. Especially, medicinal plants are very popular in this sense due to the attachment of medicinal components from plant extract to the nanoparticles corona, which could enhance the nanoparticles' efficiency for certain applications6,7,8. In addition, the waste products of plants, such as e.g. onion peels, can be used for nanoparticles synthesis, which is an especially good and sustainable production setup9,10. To expand on available venues for the synthesis of green nanoparticles, we used L. vulgare berries to prepare an aqueous extract, which was used to form stable gold and silver nanoparticles. L. vulgare is also called a common pivet or European pivet; it is a semi-evergreen and deciduous shrub. It usually grows up to 3–4 m and can be found in Europe, North Africa, and Asia. In Scandinavian countries, L. vulgare plants are grown in large amounts to make green hedges. The berries of these plants are blueish black and available in large amounts during spring. These berries germinate every year, but they are not used for food or other purposes, so they simply dry out on plants and fall off. Our study showed the possibility of using these otherwise waste and widely available biological resources to produce highly-cost products: gold and silver nanoparticles.
We chose gold and silver nanoparticles due to their high demand and numerous application in the biomedical field11,12. Gold nanoparticles are highly utilized for medical purposes like diagnostic, sensors development, photo imaging, and photothermal therapeutic applications13,14,15. Silver nanoparticles also showed immense potential for many applications in different fields such as in textile, packaging, shipping, medical, and cosmetics due to their antimicrobial property16. The antimicrobial property of silver nanoparticles has made them the first choice for various applications in which microbial contamination is a major concern. For instance, in medical devices, treating wound infections, packaging, textiles, medical device and disinfectants, etc.17,18. The high performance of silver nanoparticles creates a high demand for their production. Thus, The current study explored the L. vulgare berries as a green resource for gold and silver nanoparticles formation. We used the widely available but wasted resources to produce a high-value product.. In addition to a thorough analytical characterization for both nanoparticle types, we explored the L. vulgare berries mediated silver nanoparticles (LV-AgNPs) for antimicrobial application against two Gram-negative pathogens: P. aeruginosa and E. coli.
Materials and methods
Materials
Analytical grade gold (III) chloride trihydrate (HAuCl4·3H2O) and silver nitrate (AgNO3) were purchased from Sigma–Aldrich Chemicals (St Louis, MO, USA). To prepare the aqueous extract of L. vulgare berries, we used 10 g of fresh berries and boiled them with 90 mL of distilled water. The boiling mixture was first filtered and then centrifuged at 8000 rpm for 5 min to remove any suspended particulates19,20. The aqueous extract was diluted with different water ratios and referred to as a synthesis medium (SM).
Green synthesis of LV-AuNPs and LV-AgNPs
For the nanoparticle's synthesis, a previously reported methodology was followed20. We used the whole extract as a synthesis medium (SM) without dilution with 1 mM of gold salt (HAuCl4·3H2O) and silver salt (AgNO3) to produce LV-AuNPs and LV-AgNPs, respectively. Once the synthesis was confirmed for LV-AuNPs at room temperature and LV-AgNPs at 90 ℃, we determined the optimization factors for nanoparticles formation. For optimization studies, we used multiple dilutions of extract to autoclave water at different temperatures and ranges of salt concentrations. The optimized parameters were determined based on visual inspection and UV–Vis spectrum of formed nanoparticles.
Analytical characterization of LV-AuNPs and LV-AgNPs
UV–Vis, SEM, TEM, DLS, ICPMS, TGA, and MALDI-TOF studies were done as reported before5. Pure nanoparticles were suspended in water used for analytical characterization and application. For thermogravimetric analysis (TGA) and Fourier Transform-Infrared Spectroscopy (FT-IR), nanoparticles were air-dried to form a pellet.
Antibacterial activity of LV-AgNPs
The antimicrobial activity of LV-AgNPs was evaluated against two Gram-negative pathogens: Escherichia coli UTI 89, and Pseudomonas aeruginosa PAO1. The antimicrobial study was conducted as reported before21. Live and dead staining and SEM microscopic observation of dead and control cells were also performed as described before2.
Results
Formation of LV-AuNPs and LV-AgNPs
Visual inspection and UV–Vis spectra were used to track the formation of LV-AuNPs and LV-AgNPs. The aqueous extract of L. vulgare berries was discovered to reduce gold and silver salt to LV-AuNPs and LV-AgNPs, respectively (Fig. 1). A change in color of the SM, which is a mixture of L. vulgare berries aqueous extract, and salt solution, verified the synthesis. From the light pink color of the L. vulgare berries extract, the color of LV-AuNPs changed to dark purple, while the color of LV-AgNPs changed to brown. This color change correlates to the surface plasmon resonance (SPR) feature of the produced nanoparticles. A UV–Vis spectrum of samples in the region of 300–700 nm was used to confirm the synthesis. A defined peak was observed in the range of 500–600 nm22, for LV-AuNPs, and a maximum and broad peak was observed in the 400–500 nm for LV-AgNPs region23. After three rounds of centrifugation and DI water washing, the nanoparticle samples were scanned with UV–Vis spectroscopy. The data revealed a high-intensity peak in a similar region (Fig. 1). The optimization studies for LV-AuNPs and LV-AgNPs were also conducted by using UV–Vis spectrum analysis and visible observations. For LV-AuNPs, the optimal ratio of SM was 1:20 (Fig. 2A), HAuCl4·3H2O concentration was 2 mM (Fig. 2B), and the reaction took place at room temperature within 5 min. For LV-AgNPs, the optimal ratio of SM for nanoparticles production was 1:9 (Fig. 2D), temperature 90 °C (Fig. 2E), 5 mM silver salt concentration (Fig. 2F), and synthesis time noted was 15 min (Fig. 2G).
Characterization of LV-AuNPs and LV-AgNPs
To investigate the detailed morphological features of LV-AuNPs and LV-AgNPs, the purified and concentrated samples of nanoparticles were studied by SEM, EDX, elemental mapping, TEM, and SAED. SEM displayed the spherical, triangular, hexagonal rods; cuboid shapes for LV-AuNPs, which cause polydispersities (Fig. 3A,B). In contrast, for LV-AgNPs, we found a spherical form in the majority of the population (Fig. 3C,D). In addition, we conducted EDX, and elemental mapping, which showed a clear map of gold and silver elements in LV-AuNPs (Fig. 3E–G) and LV-AgNPs scanned micrographs (Fig. 3I–K). EDX displayed the clear peak of gold and silver elements, confirming the maximum distribution of gold (Fig. 3H) and silver elements (Fig. 3L) in the respective samples24. TEM image analysis revealed the different shapes and confirmed that an organic layer derived from the berries extract covers the nanoparticles. In addition, TEM analysis also revealed the different shapes for LV-AuNPs with a core diameter of 50–200 nm (Fig. 3M,N) and LV-AgNPs with size 20–70 nm (Fig. 3Q,R), respectively. The SAED pattern corresponds to the main reflection lattice planes of (111), (200), (220), and (311), which represents the polycrystalline nature of LV-AuNPs (Fig. 3O,P) and LV-AgNPs (Fig. 3S,T)25,26.
The hydrodynamic diameter and zeta potential values of the nanoparticles were then measured using DLS. LV-AuNPs (Fig. 4A) had a size of 292.3 nm and a polydispersity index (PDI) of 0.3, while LV-AgNPs (Fig. 4B) had a size of 542.6 nm and a PDI of 0.47927. The zeta potential of as-prepared nanoparticles dispersed in water at ambient temperature showed negative values for LV-AuNPs − 18.3 mV (Fig. 4C) and LV-AgNPs − 20.8 mV (Fig. 4D), indicating negative surface charge6.
Next, sp-ICPMS analysis was used to determine the nanoparticle's concentration and stability over time. The concentration of LV-AuNPs was 2.23 g/L (Fig. 5A), while the concentration of LV-AgNPs was 0.615 g/L. (Fig. 5D). After two weeks (Fig. 5B,E) and one year (Fig. 5F), the same samples were tested again (Fig. 5C,F). The collected data showed a consistent histogram with no changes, indicating that the produced nanoparticles remained in the same size and concentration for up to a year, indicating that they were highly stable20. UV–Vis analysis was also used to test the stability of nanoparticles at different times and in different mediums. The UV–Vis scan of nanoparticles in a difference of two weeks showed a peak in the same region (Fig. 6A,B). The results of the medium stability test demonstrated that the LV-AuNPs (Fig. 6C) and LV-AgNPs (Fig. 6D) remain stable in all three mediums. Furthermore, TGA was used to conduct the temperature stability, and the results showed complete nanoparticle degradation with increasing temperature (Fig. 6E,F).
Furthermore, MALDI-TOF was conducted to examine gold and silver ions on nanoparticle´s surface (Fig. 7A,B). The mass spectra displayed intense single peaks between 590 and 3800 m/z. For LV-AuNPs, several peaks were observed which belong to gold ions, at 590.699, 985.041, 1576.076, 2166.315, 2560.312, 3151.497, 3347.342, and 3938.624 (Fig. 7A). For LV-AgNPs, many peaks were found which could be assigned to silver ions of higher cationic species at 647.392, 754.507, 863.457, 968.370, 1186.233, 1402.093, 1617.962 and1833.728 (Fig. 7B)5.
Antibacterial activity of LV-AgNPs
LV-AgNPs were explored for the antibacterial activity against P. aeruginosa and E. coli. LV-AgNPs completely killed the P. aeruginosa cells at 150 µg/mL and E. coli cells at 100 µg/mL (Fig. 8). The cell viability was further confirmed by using the live and dead staining techniques. P. aeruginosa and E. coli cells treated with different concentrations of LV-AgNPs were stained with live and dead reagents and observed with a fluorescent microscope (Fig. 9). For P. aeruginosa (Fig. 9A–H), many cells were viable at 32–50 µg/mL of LV-AgNPs, but with increasing concentration, the green (live) cells were no longer visible. Concomitantly, the red signal coming from dead cells became increasingly dense, meaning that the LV-AgNPs were toxic to cells and caused the complete death of P. aeruginosa cells at 150 µg/mL. Similar results were obtained with E. coli cells (Fig. 9I–R), which died completely at 100 µg/mL concentration of LV-AgNPs.
SEM was also used to examine the morphology of the dead cells. P. aeruginosa cells treated with LV-AgNPs were analyzed by SEM at two concentrations: 50 and 100 g/mL. P. aeruginosa cells were totally covered in nanoparticles and had disrupted membrane structures, as seen in Fig. 10D–F. Figure 11N–P shows a similar response at a 100 g/mL concentration of LV-AgNPs. The elemental mapping results also showed that the injured cells were totally covered in LV-AgNPs (Fig. 10G–I,Q–S), confirming that LV-AgNPs were the cause of cell lysis. Furthermore, in injured cells, EDX revealed a clear peak for silver element (Fig. 10J,T), confirming this hypothesis. Figure 11 shows the effects of LV-AgNPs on E. coli cells. Pathogenic cells show the open cellular structure with damaged membrane and are covered under the nanoparticles. The EDX and elemental mapping results indicated that the cells were covered predominantly with silver, indicating that the cellular damage occurred due to the silver ions released from LV-AgNPs Fig. 11G–I,Q–S.
Discussion
Our method uses the aqueous extract of L. vulgare berries to reduce gold and silver salt into nanoparticles. For both LV-AuNPs and LV-AgNPs, the dark purple and deep brown color generated in the SM is a clear sign of the nanoparticle's appearance. The nanoparticles were purified and displayed again overlapping absorbance with higher intensity in the UV–Vis spectrum. Optimization studies for LV-AuNPs, demonstrated that the diluted berries extract (1:20 dilution) offers the best conditions for LV-AuNPs production. With such high dilutions, it is possible to produce many gold nanoparticles using very little biological resources. Additionally, the process did not require any additional reducing or stabilizing agents; the aqueous extract was enough to do the job. The gold salt optimization study revealed an optimum peak at 2 mM concentration. Any further increase in salt level causes peak shifting and broadening, which means that the nanoparticles lost their structures by precipitating. The optimum reduction time absorbed was 5 min for the time performance case. Keeping the SM under the same conditions caused nanoparticles to settle, as clearly visible in Fig. 2C. Until five minutes, nanoparticles solution appeared to be homogenous, and peaks also showed a fine and narrow appearance. For the LV-AgNPs optimization study, 1:10 (100 µL) of extract diluted with water displayed optimum production, which is advantageous because synthesis used very few amounts of resources. The temperature study showed 90 ℃ is the optimum temperature with the highest peak in UV–Vis absorbance and homogeneity in solution Fig. 2E. 5 mM of silver salt came out as the optimum salt concentration for completely reducing silver salt to nanoparticles within 15 min. All the optimization study UV–Vis peaks align with the visible picture of SMs shown below each graph.
The structural morphology of nanoparticles showed a big difference between the TEM and DLS size analysis, especially for LV-AgNPs. It is well known that the size difference between the two methods is due to the fact that TEM shows core nanoparticles size and DLS shows hydrodynamic diameter. However, despite the two values being overall correlated, the big difference most likely reflects a large amount of biological components around the surface of the nanoparticles, which form the corona layer. For any nanoparticles applications, stability is the utmost requirement 28. The corona layer helped our nanoparticles to maintain their structure and stability for more than one year in different media and solutions, as shown in the ICPMS study (Fig. 5) and stability analysis study (Fig. 6). ICPMS pattern of LV-AuNPs displays a similar distribution of nanoparticles between 17 and 250 nm in a difference of two weeks and after one year (Fig. 5A–C). LV-AgNPs also exhibited a similar behavior by showing the maximum frequency of nanoparticles in size range 15–100 nm for fresh samples and keeping the nanoparticles solution for two weeks and one year (Fig. 5D–F). UV–Vis spectrum-based analysis of two weeks incubation and incubation in different media also revealed overlapping patter in peaks, which means nanoparticles were not affected by changing media solutions or time. This high level of stability was achieved due to the surrounding corona layer that forms during synthesis without adding any additional stabilizer and remains with nanoparticles for a prolonged period and at ambient conditions29. In addition, the negative zeta potential value also showed that the nanoparticles are stabilized by electrostatic repulsion and steric hindrance of organic moieties present in the L. vulgare berries extract. The TGA graph shows that the nanoparticles are degradable at higher temperatures despite the corona layer. From 200 to 400 ℃, the corona layers start depleting slowly and further increase in temperature more than 400 ℃ causes complete nanoparticles destruction.
Any bioactive property exhibited by nanoparticles depends on their physical properties, such as shape and size, which are largely determined by the conditions of synthesis30. LV-AgNPs were explored for antibacterial study against P. aeruginosa and E. coli. Results showed an MBC value of 150 µg/mL against P. aeruginosa and 100 µg/mL against E. coli, higher than the values reported in previous studies where similar systems were explored. For instance, Patra et al. showed the MBC value of silver nanoparticles produced using corn leaf waste of Zea mays, as 100 µg/mL against E. coli31, similar to our case. Loo et al. showed the MBC value of silver nanoparticles from pu-erh tea leaves extract against E. coli as 7.8 µg/mL32. Khorrami et al. demonstrated the walnut husk mediated silver nanoparticles MBC value against P. aeruginosa and E. coli as 20 and 5 µg/mL33. We concluded that the weak antimicrobial activity (high MBC values) compared to the examples mentioned above could be due to the think corona layer, which provides less toxicity butlong-term stability. The extreme thickness of corona could be judged by the size difference between TEM (20–70 nm) and DLS (542.6 nm) for LV-AgNPs. The membrane is also visible in the TEM image of LV-AgNPs (Fig. 3S,T). The live and dead staining experiment also followed the similar pattern of plate assay results and showed maximum green cells (live) at 32 µg/mL and 50 µg/mL in P. aeruginosa; however, the green intensity reduces and red increases at 100 and 150 µg/mL, which means more cells are dead than alive. For E. coli, at 50 and 100 µg/mL, most cells appeared to be dead due to LV-AgNPs effects.
The SEM morphological analysis of treated cells displayed the complete open structure of individual cells with the nanoparticles surrounding them (Fig. 10D–F,N–P). The image demonstrates that cells died due to the lysis and membrane damage caused by LV-AgNPs action. The small size of LV-AgNPs could also help the nanoparticles internalize and damage the other cellular structures, such as DNA, proteins, ribosomes, etc.34,35. The other possibility of cell death is the generation of reactive oxygen species (ROS) once the nanoparticles are internalized, a well-known mechanism for the silver nanoparticles' antimicrobial effects30,36. Elemental mapping scanning of the nanoparticles surrounded cells also showed a clear and sharp peak for silver elements in the EDX spectrum and distribution (blue) in mapping analysis (Fig. 10G–I,Q–S). Comparatively, the control cells remain intact without any damage. Figure 11D–F,N–P displayed the E. coli cells damaged and formed hollow structures, which resemble complete lysis due to the LV-AgNPs action at two concentrations 50 and 100 µg/mL. The porins present in the membrane of Gram-negative bacteria are large enough to allow the passive diffusion of small nanoparticles. This represents another possibility to allow nanoparticles uptake by the cells, leading to internal damage in addition to membrane leakage. The destruction caused by silver ions released from nanoparticles was further confirmed by the elemental mapping and EDX analysis, which resemble silver element distribution and sharp peak at 3 keV (Fig. 11G–I,Q–S). Our reports are in alignment with previously published results.
The important and noticeable feature in the antimicrobial study of nanoparticles is that the LV-AgNPs remain intact in their appearance after killing the pathogenic cells. Figure 10F,O,P for P. aeruginosa cells and Fig. 11D,F,O,P for E. coli cells shows the dead cells with open structures with spherical silver nanoparticles surrounding them. This shows that nanoparticles remain stable even after delivering their action either intracellularly or extracellularly and manage to come out of cells after complete lysis. Again, the thick corona layer helps them maintain their identity without losing any stability. The question is would it be possible to retrieve these nanoparticles and apply them again for a similar purpose. Since the nanoparticle came in contact with a living organism that changed the corona composition, would the LV-AgNPs with the new corona layer have the same effect? These questions are remained to answer in future research and probably solve the doubts about the reusability of nanoparticles and their environmental impact.
Conclusions
We used L. vulgare berries for the efficient production of gold and silver nanoparticles in a rapid, efficient, and economical method. L. vulgare berries are mostly not used for any purpose and hence considered a waste. This study demonstrates the use of a waste resource to produce a value-added product. L. vulgare berries to manufacture commercial nanoparticles will assist in making the process more sustainable by lowering costs and repurposing waste. The results showed that the biological components from L. vulgare berries extract played a critical role in completely reducing and stabilizing nanoparticles by forming a transparent layer around them. LV-AgNPs showed antimicrobial potential with an MBC value of 150 µg/mL against P. aeruginosa and 100 µg/mL against E. coli.
References
Singh, P., Kim, Y.-J., Zhang, D. & Yang, D.-C. Biological synthesis of nanoparticles from plants and microorganisms. Trends Biotechnol. 34, 588–599. https://doi.org/10.1016/j.tibtech.2016.02.006 (2016).
Singh, P. et al. Silver nanoparticles produced from Cedecea sp. exhibit antibiofilm activity and remarkable stability. Sci. Rep. 11, 12619. https://doi.org/10.1038/s41598-021-92006-4 (2021).
Kapoor, R. T. et al. Exploration of microbial factories for synthesis of nanoparticles—A sustainable approach for bioremediation of environmental contaminants. Front. Microbiol. 12, 658294. https://doi.org/10.3389/fmicb.2021.658294 (2021).
Singh, P., Pandit, S., Mokkapati, V., Garnaes, J. & Mijakovic, I. A sustainable approach for the green synthesis of silver nanoparticles from Solibacillus isronensis sp. and their application in biofilm inhibition. Molecules https://doi.org/10.3390/molecules25122783 (2020).
Singh, P. et al. Green synthesis of gold and silver nanoparticles from Cannabis sativa (industrial hemp) and their capacity for biofilm inhibition. Int. J. Nanomed. 13, 3571–3591. https://doi.org/10.2147/IJN.S157958 (2018).
Singh, P. et al. Anti-biofilm effects of gold and silver nanoparticles synthesized by the Rhodiola rosea rhizome extracts. Artif. Cells Nanomed. Biotechnol. 46, S886–S899. https://doi.org/10.1080/21691401.2018.1518909 (2018).
Abbai, R. et al. Green synthesis of multifunctional silver and gold nanoparticles from the oriental herbal adaptogen: Siberian ginseng. Int. J. Nanomed. 11, 3131–3143. https://doi.org/10.2147/IJN.S108549 (2016).
Chandra, H., Kumari, P., Bontempi, E. & Yadav, S. Medicinal plants: Treasure trove for green synthesis of metallic nanoparticles and their biomedical applications. Biocatal. Agric. Biotechnol. 24, 101518. https://doi.org/10.1016/j.bcab.2020.101518 (2020).
Patra, J. K., Kwon, Y. & Baek, K.-H. Green biosynthesis of gold nanoparticles by onion peel extract: Synthesis, characterization and biological activities. Adv. Powder Technol. 27, 2204–2213. https://doi.org/10.1016/j.apt.2016.08.005 (2016).
Singhal, A. & Gupta, A. Sustainable synthesis of silver nanoparticles using exposed X-ray sheets and forest-industrial waste biomass: Assessment of kinetic and catalytic properties for degradation of toxic dyes mixture. J. Environ. Manage. 247, 698–711. https://doi.org/10.1016/j.jenvman.2019.06.078 (2019).
Singh, P. & Mijakovic, I. Advances in gold nanoparticle technology as a tool for diagnostics and treatment of cancer. Expert Rev. Mol. Diagn. 21, 627–630. https://doi.org/10.1080/14737159.2021.1933447 (2021).
Ullah Khan, S. et al. Nanosilver: New ageless and versatile biomedical therapeutic scaffold. Int. J. Nanomed. 13, 733–762. https://doi.org/10.2147/IJN.S153167 (2018).
Kumar, A., Zhang, X. & Liang, X. J. Gold nanoparticles: Emerging paradigm for targeted drug delivery system. Biotechnol. Adv. 31, 593–606. https://doi.org/10.1016/j.biotechadv.2012.10.002 (2013).
Kumar, A. et al. Gold nanoparticles functionalized with therapeutic and targeted peptides for cancer treatment. Biomaterials 33, 1180–1189. https://doi.org/10.1016/j.biomaterials.2011.10.058 (2012).
McQuaid, H. N. et al. Imaging and radiation effects of gold nanoparticles in tumour cells. Sci. Rep. 6, 19442. https://doi.org/10.1038/srep19442 (2016).
Burdusel, A. C. et al. Biomedical applications of silver nanoparticles: An up-to-date overview. Nanomaterials (Basel). https://doi.org/10.3390/nano8090681 (2018).
Deshmukh, S. P., Patil, S. M., Mullani, S. B. & Delekar, S. D. Silver nanoparticles as an effective disinfectant: A review. Mater. Sci. Eng. C Mater. Biol. Appl. 97, 954–965. https://doi.org/10.1016/j.msec.2018.12.102 (2019).
Gherasim, O., Puiu, R. A., Birca, A. C., Burdusel, A. C. & Grumezescu, A. M. An updated review on silver nanoparticles in biomedicine. Nanomaterials (Basel). https://doi.org/10.3390/nano10112318 (2020).
Singh, H., Du, J., Singh, P. & Yi, T. H. Ecofriendly synthesis of silver and gold nanoparticles by Euphrasia officinalis leaf extract and its biomedical applications. Artif. Cells Nanomed. Biotechnol. 46, 1163–1170. https://doi.org/10.1080/21691401.2017.1362417 (2018).
Singh, P. & Mijakovic, I. Rowan berries: A potential source for green synthesis of extremely monodisperse gold and silver nanoparticles and their antimicrobial property. Pharmaceutics 14, 82 (2022).
Singh, P. & Mijakovic, I. Strong antimicrobial activity of silver nanoparticles obtained by the green synthesis in Viridibacillus sp. extracts. Front. Microbiol. https://doi.org/10.3389/fmicb.2022.820048 (2022).
Singh, P., Kim, Y. J., Wang, C., Mathiyalagan, R. & Yang, D. C. Microbial synthesis of flower-shaped gold nanoparticles. Artif. Cells Nanomed. Biotechnol. 44, 1469–1474. https://doi.org/10.3109/21691401.2015.1041640 (2016).
Singh, H., Du, J., Singh, P. & Yi, T. H. Extracellular synthesis of silver nanoparticles by Pseudomonas sp. THG-LS1.4 and their antimicrobial application. J. Pharm. Anal. 8, 258–264. https://doi.org/10.1016/j.jpha.2018.04.004 (2018).
Singh, P. et al. Biogenic silver and gold nanoparticles synthesized using red ginseng root extract, and their applications. Artif. Cells Nanomed. Biotechnol. 44, 811–816. https://doi.org/10.3109/21691401.2015.1008514 (2016).
Singh, P., Kim, Y. J., Wang, C., Mathiyalagan, R. & Yang, D. C. The development of a green approach for the biosynthesis of silver and gold nanoparticles by using Panax ginseng root extract, and their biological applications. Artif. Cells Nanomed. Biotechnol. 44, 1150–1157. https://doi.org/10.3109/21691401.2015.1011809 (2016).
Singh, P., Kim, Y. J. & Yang, D. C. A strategic approach for rapid synthesis of gold and silver nanoparticles by Panax ginseng leaves. Artif. Cells Nanomed. Biotechnol. 44, 1949–1957. https://doi.org/10.3109/21691401.2015.1115410 (2016).
Singh, P. et al. Extracellular synthesis of silver and gold nanoparticles by Sporosarcina koreensis DC4 and their biological applications. Enzyme Microb. Technol. 86, 75–83. https://doi.org/10.1016/j.enzmictec.2016.02.005 (2016).
Belteky, P. et al. Silver nanoparticles: Aggregation behavior in biorelevant conditions and its impact on biological activity. Int. J. Nanomed. 14, 667–687. https://doi.org/10.2147/IJN.S185965 (2019).
Durán, N., Silveira, C. P., Durán, M. & Martinez, D. S. T. Silver nanoparticle protein corona and toxicity: A mini-review. J. Nanobiotechnol. 13, 55. https://doi.org/10.1186/s12951-015-0114-4 (2015).
Dakal, T. C., Kumar, A., Majumdar, R. S. & Yadav, V. Mechanistic basis of antimicrobial actions of silver nanoparticles. Front. Microbiol. https://doi.org/10.3389/fmicb.2016.01831 (2016).
Patra, J. K. & Baek, K.-H. Antibacterial activity and synergistic antibacterial potential of biosynthesized silver nanoparticles against foodborne pathogenic bacteria along with its anticandidal and antioxidant effects. Front. Microbiol. https://doi.org/10.3389/fmicb.2017.00167 (2017).
Loo, Y. Y. et al. In vitro antimicrobial activity of green synthesized silver nanoparticles against selected gram-negative foodborne pathogens. Front. Microbiol. 9, 1555 (2018).
Khorrami, S., Zarrabi, A., Khaleghi, M., Danaei, M. & Mozafari, M. R. Selective cytotoxicity of green synthesized silver nanoparticles against the MCF-7 tumor cell line and their enhanced antioxidant and antimicrobial properties. Int. J. Nanomed. 13, 8013–8024. https://doi.org/10.2147/IJN.S189295 (2018).
Tang, S. & Zheng, J. Antibacterial activity of silver nanoparticles: Structural effects. Adv. Healthcare Mater. 7, 1701503. https://doi.org/10.1002/adhm.201701503 (2018).
Karade, V. C. et al. Insights into shape-based silver nanoparticles: A weapon to cope with pathogenic attacks. ACS Sustain. Chem. Eng. 9, 12476–12507. https://doi.org/10.1021/acssuschemeng.1c03797 (2021).
Joshi, A. S., Singh, P. & Mijakovic, I. Interactions of gold and silver nanoparticles with bacterial biofilms: Molecular interactions behind inhibition and resistance. Int. J. Mol. Sci. https://doi.org/10.3390/ijms21207658 (2020).
Acknowledgements
ICPMS was performed at DTU Environmental; TEM was performed at the Center for electron microscopy; DTU. The authors acknowledge Dr. Abida Sultan for conducting the MALDI-TOF experiment.
Funding
Lundbeckfonden to P.S. (R303-2018-3499), Novo Nordisk Foundation (NNF10CC1016517) and the NordForsk (project number 105121) to I.M.
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P.S. designed and performed all the experiments, analyzed the results, and prepared the manuscript and figures. I.M. supervised all experimental work and edited the manuscript.
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Singh, P., Mijakovic, I. Green synthesis and antibacterial applications of gold and silver nanoparticles from Ligustrum vulgare berries. Sci Rep 12, 7902 (2022). https://doi.org/10.1038/s41598-022-11811-7
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DOI: https://doi.org/10.1038/s41598-022-11811-7
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