Abstract
Long non-coding RNAs (lncRNAs) are transcripts >200 nucleotides that have prominently surfaced as dynamic regulatory molecules. Using computational approaches, we identified and characterized 56,441 lncRNAs in grapevine (Vitis vinifera) by harnessing RNA-seq data from 10 developmental stages of leaf, inflorescence, and berry tissues. We conducted differential expression analysis and determined tissue- and developmental stage-specificity of lncRNAs in grapevine, which indicated their spatiotemporal regulation. Functional annotation using co-expression analysis revealed their involvement in regulation of developmental transitions in sync with transcription factors (TFs). Further, pathway enrichment analysis revealed lncRNAs associated with biosynthetic and secondary metabolic pathways. Additionally, we identified 115, 560, and 133 lncRNAs as putative miRNA precursors, targets, and endogenous target mimics, respectively, which provided an insight into the interplay of regulatory RNAs. We also explored lncRNA-mediated regulation of extra-chromosomal genes–i.e., mitochondrial and chloroplast coding sequences and observed their involvement in key biological processes like ‘photosynthesis’ and ‘oxidative phosphorylation’. In brief, these transcripts coordinate important biological functions via interactions with both coding and non-coding RNAs as well as TFs in grapevine. Our study would facilitate future experiments in unraveling regulatory mechanisms of development in this fruit crop of economic importance.
Similar content being viewed by others
Introduction
Long non-coding RNAs (lncRNAs) are regulatory non-protein coding transcripts primarily characterized by their length, that is, more than 200 nucleotides in general. These are normally expressed in low levels but in tissue- and cell-type-specific fashion1. lncRNAs are predominantly localized in the nucleus2 and are known to be poorly conserved at the sequence level during evolution3. The biogenesis of lncRNAs is very similar to that of the messenger RNAs (mRNAs). Most lncRNAs are transcribed by RNA polymerase II in eukaryotes and undergo typical post-transcriptional modifications such as 5′ capping, polyadenylation, and splicing, while some are also transcribed by RNA polymerase III4,5. Particularly in plants, RNA polymerases IV and V have additionally been observed to transcribe lncRNAs. In fact, an exclusive class of lncRNAs transcribed as products of the plant-specific RNA polymerase V has been reported to be involved in RNA-directed DNA methylation6,7. Further, based on their genomic locations with respect to their adjoining protein-coding genes, lncRNAs are categorized into distinct classes such as intergenic, intronic, sense, anti-sense, and bidirectional lncRNAs3.
The discovery of lncRNAs in mammals and plants was contemporaneous; however, over the decades, the progress of lncRNA research in the former, particularly in humans, proved trailblazing. An extensive literature supports the involvement of lncRNAs in complex human diseases8 such as cancer9, cardiovascular disorders like myocardial infarction10, and autoimmune disorders like rheumatoid arthritis11 and psoriasis12. In contrast to the numerous case studies in humans, roles of limited lncRNAs have been revealed in plants. For instance, anti-sense and intronic lncRNAs COOLAIR and COLDAIR, respectively, epigenetically silence the key flowering repressor FLOWERING LOCUS C (FLC) in the vernalization-dependent pathway13,14. Other important lncRNAs are LDMAR, IPS1, ENOD40, ASCO-lncRNA, APOLO, and NERDL, which have been characterized as regulators of biological processes like fertility, phosphate homeostasis, nodule formation, lateral root development, auxin-controlled development, and secondary wood formation, respectively15,16,17,18,19,20,21. As per recent reviews, genome-wide plant lncRNA research has lately re-emerged owing to the widespread application of the next-generation RNA-sequencing. Presently, thousands of novel lncRNAs have been predicted in silico in several plant species in response to different conditions such as stress (biotic and abiotic), nutrient starvation, and hormone treatments22,23. Further, well-established studies in animals and few yet promising studies in plants have highlighted the plausible involvement of lncRNAs in tissue development-a vital biological phenomenon24,25,26,27,28,29,30.
In the present study, we harnessed RNA-seq data for different tissues of Vitis vinifera (grapevine) at various developmental stages and identified 56,441 putative lncRNAs via a computational pipeline. V. vinifera is an economically and nutritionally important perennial fruit crop, for which considerable genomic and transcriptomic information is available; hence, conducting genome-based global analysis is feasible in the plant. Several “omics” studies in V. vinifera have primarily focused on understanding the role and interplay of DE protein-coding genes, metabolites, and proteins at different developmental stages, particularly at key points during mature berry development31,32,33. Through this study, we tried to explore the DE lncRNAs during different developmental stages and identify their possible biological roles in the plant. Additionally, we investigated relationships among the elements of the non-coding genome (like lncRNAs and micro RNAs [miRNAs]) and the protein-coding mRNAs. Since lncRNAs regulate key biological processes in other organisms including various plant species, it has been necessary to investigate their potential functions in different tissues and developmental stages of the most widely cultivated species of grapevine, V. vinifera. Our study is a step in this direction and provides information to facilitate the process of understanding the underlying mechanisms of gene expression with emphasis on lncRNAs as new but important players.
Results
Genome-wide Identification of lncRNAs in V. vinifera
The RNA-seq data (≈200GB) retrieved for leaf, inflorescence, and berry tissues at 10 different developmental stages were assembled into 3,59,570 contigs using Trinity package34. Different classical filters were applied to identify the potential lncRNAs. A total of 2,51,439 contigs having open reading frames (ORFs) encoding proteins greater than 100 amino acids in six frame translation were eliminated. The coding potential analyses using CPC35 filtered additional 14 transcripts. Homology search filter was applied on the remaining 1,08,117 transcripts by performing BLASTX analysis against the NCBI NR protein database, which ultimately resulted into the identification of final 56,441 transcripts as putative lncRNAs. As a cursory check of our computational approach, we performed standalone BLASTN for these 56,441 putative lncRNAs with respect to lncRNAs available on CANTATAdb36 for V. vinifera that revealed hits for 7,262 putative lncRNAs, which indicates that our computational approach is reliable. Additionally, this suggests that out of the identified putative lncRNAs, 49,179 lncRNAs are new.
Characteristics of the Identified V. vinifera lncRNAs
Different characteristics of the predicted lncRNAs were examined to understand the trends of their occurrence in the genome. These characteristics were also studied for the mature mRNAs in order to juxtapose the two different transcript categories.
Chromosomal Distribution
Out of 56,441 predicted lncRNAs, we could determine the chromosomal localization of 52,144 lncRNAs, which were unevenly distributed across the 19 chromosomes (Fig. 1A). The highest (7.5%) and lowest (3.6%) proportion of lncRNAs were located on chromosome 18 and chromosome 10, respectively. Similar distribution pattern was observed for the 37,420 mature mRNAs with the highest (7.39%) and lowest (3.45%) proportions on chromosomes 18 and 10, respectively.
Chromosomal Density
The chromosomal density analysis revealed that lncRNAs were more densely present on V. vinifera chromosomes than the mature mRNAs (Fig. 1B). Chromosome 8 had the highest lncRNA density with 165.61 lncRNAs per Mbp of nucleotides, whereas chromosome 9 had the lowest density with 91.15 lncRNAs per Mbp of nucleotides. Interestingly, the maximum and minimum chromosomal densities for mature mRNAs were also observed on chromosome 8 with 107.02 mRNAs per Mbp of nucleotides and chromosome 9 with 69.11 mRNAs per Mbp of nucleotides, respectively. Moreover, similar density pattern was observed for the both transcript categories despite the differences in the density magnitude.
Length Distribution
The length of lncRNAs ranged from 224 bp to 3,910 bp; nevertheless, length of more than half of the lncRNAs (54.3%) varied between 250 to 500 bp (Fig. 1C).
In addition to these characteristics, the lncRNAs were classified based on their genomic locations with respect to those of the neighboring protein-coding genes (Fig. 1D). The majority of the identified lncRNAs, that is, 30%, 29.27%, and 13.72% were classified as intergenic (transcripts mapped to the unknown intergenic regions), intronic (transcripts mapped completely within the introns of the known protein-coding genes), and antisense (transcripts mapped to the exon of a protein-coding gene but on the opposite strand) lncRNAs, respectively.
Expression Analyses and Estimation of Tissue and Developmental Stage Specificity of the lncRNAs
To identify the differentially expressed (DE) V. vinifera lncRNAs across the inflorescence, berry, and leaf tissues under 10 developmental stages, FPKM values were determined using RSEM37 and edgeR38 as a part of the Trinity software package. On applying a threshold cut-off of P-values (FDR) < = 0.001 and 4-fold change, 7697 DE lncRNAs were identified (Fig. 2A). A maximum of 7479 DE lncRNAs was found in inflorescence tissue at the developmental stage of 5 days post 100% cap-fall. The lowest number of lncRNAs (4172) was expressed in berry tissue at the intermediate stage of ripening, which corresponds to E-L stage 36. On an average, 7454, 5088, and 5933 lncRNAs were expressed in inflorescence, berry, and leaf tissues, which indicated the plausible importance of those lncRNAs in reproductive tissues particularly from flower to fruit development. Further, based on the applied parameters, 5411 DE mature mRNAs were obtained. Of the total mRNAs and lncRNAs studied here, approximately 14.5% and 13.6% transcripts exhibited differential expression patterns across the different tissues and developmental stages, respectively, (Supplementary Fig. 1). Additionally, based on the expression levels, the lncRNAs were categorized into 7 groups: (1) extremely low (FPKM, >0 and <= 5); (2) very low (FPKM, >5 and <= 20); (3) low (FPKM, >20 and <= 50); (4) moderate (FPKM, >50 and <= 100); (5) high (FPKM, >100 and <= 500); (6) very high (FPKM, >500 and <= 1000); and (7) extremely high (FPKM, >1000) (Fig. 2B). More than 70% of the lncRNAs were expressed between moderate and extremely low levels in each tissue/developmental stage. The “high to extremely high expressing” lncRNAs suggested the possibility of a biological rationale in certain tissues at specific stages of development.
Based on the method described by Julien et al., we calculated specificity indices for the inflorescence, berry, and leaf tissues at different developmental stages39. On applying a cut-off of 0.7 as the threshold specificity index value, a total of 142 highly tissue and developmental stage specific lncRNAs were identified (Fig. 2C). The maximum proportion (34%) of developmental stage-specific lncRNAs was observed in the mature leaf tissue. Moreover, half of the mature leaf-specific lncRNAs belonged to the “high to extremely high expression” groups. Overall, 40% of the tissue-specific lncRNAs were determined in the inflorescence tissue; more than half (57.5%) of them were found to be specific for the stage of 7 days after 100% cap-fall. Likewise, 22% of the tissue-specific lncRNAs were observed in the berry tissue; 59% of them were specific to the mature berry stage. Variable expression levels across the tissues at distinct developmental stages were observed in the expression heatmap of tissue-specific lncRNAs (Fig. 2D).
Functional Annotation of the lncRNAs
In order to annotate the putative functions of the lncRNAs, we conducted co-expression analysis of these transcripts with the mature mRNAs. Firstly, 56,441 lncRNAs and 37,420 mRNAs were filtered to eliminate the transcripts with an average expression <30 FPKM value. Consequently, 9,933 lncRNAs and 6,104 mRNAs were analyzed. Co-expression correlation between lncRNAs and mature mRNAs was calculated using Pearson correlation with R2 ≥ 0.9. We found that 6,628 lncRNAs co-expressed with 2,010 mature mRNAs (Supplementary Table 1). GO enrichment analysis was performed for these 2,010 mRNAs using Blast2GO40, and nearly 95% of these could be functionally annotated with at least one GO term in the following three categories: cellular component (example, GO:0005634 ‘nucleus’ [30.04% sequences], GO:0005737 ‘cytoplasm’ [70.82% sequences]); molecular functions (example, GO:0005488 ‘binding’ [69.56% sequences], GO:0003824 ‘catalytic activity’ [58.67% sequences]); and biological processes (example, GO:0050896 ‘response to stimulus’ [40.56% sequences], GO:0032502 ‘developmental process’ [18.76% sequences]) (Supplementary Table 2). Figure 3 highlights the top terms for each category. Further data mining on annotation results was conducted using Blast2GO, and enzyme codes (EC) were obtained for the annotated sequences accordingly (Supplementary Fig. 2). The EC distribution pattern revealed that out of the six major EC classes, the maximum co-expressing lncRNA-mRNA pairs belonged to hydrolases, transferases, and oxidoreductases classes. Subsequently, we conducted the pathways enrichment analysis using KEGG pathways database, and the results indicated that lncRNAs are potentially involved in representatives of 121 pathways (Supplementary Table 3). Among these, based on the number of highly enriched enzymes, lncRNAs are likely to be involved in regulation of the following pathways: ‘biosynthesis of antibiotics’, ‘purine metabolism’, ‘starch and sucrose metabolism’, ‘cysteine and methionine metabolism’, ‘glycolysis/gluconeogenesis’, ‘amino sugar and nucleotide sugar metabolism’, ‘porphyrin and chlorophyll metabolism’, ‘phenylalanine, tyrosine and tryptophan biosynthesis’, ‘carbon fixation in photosynthetic organisms’, and ‘flavonoid biosynthesis’. Interestingly, this analysis also revealed the potential involvement of 10 and 14 lncRNAs in regulation of two key processes for the plant: photosynthesis and oxidative phosphorylation, respectively (Supplementary Fig. 3).
Interactions between lncRNAs and Transcription Factors in V. vinifera
Since the sequence information for the TFs known in V. vinifera was available at Plant TF database (PlantTFDB) v4.0, we explored the potential association of these regulatory players with the predicted lncRNAs by conducting co-expression analysis. We identified 62 TFs belonging to 19 TF superfamilies and families that were co-expressed with lncRNAs across the different tissues and developmental stages (Fig. 4). Of these, APETALA2/Ethylene Responsive Factor (AP2/ERF) superfamily, which is known to be conservatively widespread in the plant kingdom, was found to be highly enriched (17.7%). The other TFs that were highly co-expressed belonged to WRKY (14.5%) and MYB (11.3%) TF families.
Interactions of lncRNAs with mRNAs and miRNAs in V. vinifera
V. vinifera-specific mature miRNAs were also downloaded from mirBase41 (miRNA database). Using plant small RNA target analysis server, psRNATarget, we identified miRNA target sites in the lncRNAs. Our analyses revealed 560, 115, and 133 lncRNAs as putative micro RNA (miRNA) targets, precursors, and endogenous target mimics (eTMs), respectively (Fig. 5A, Supplementary Table 4). We identified lncRNAs that potentially interact with miRNAs known to play important roles in plant developmental processes such as vvi-miR166, vvi-miR156, vvi-miR172, and vvi-miR319. Figure 5(B–D,E,F) represent the examples of secondary structure prediction and interaction analyses, respectively. The secondary structures shown in Fig. 5 are cropped images used for better display purpose. For viewing the complete image of the secondary structures, refer to Supplementary Fig. 4. The intricacy of the interactome comprising lncRNAs, miRNAs, and mRNAs can be seen in the Supplementary Fig. 5.
Potential Regulation of Genes of Chloroplast and Mitochondrion by lncRNAs
In order to inspect whether the lncRNAs shared homology with the extra-chromosomal genome of the plant, we conducted a standalone BLASTN analysis (length of alignment, >200 nt). We obtained 52 and 139 hits for lncRNAs with respect to the chloroplast and mitochondrial genome, respectively. Further, the co-expression analysis of the 56,441 lncRNAs was performed with the 84 chloroplast and 74 mitochondrial CDS, separately. A total of 1,753 and 1590 lncRNAs were found to co-express with 38 chloroplast and 25 mitochondrial CDS, respectively. The highly enriched GO terms associated with the co-expressed chloroplast CDS included photosynthesis (GO:0015979), cellular metabolic process (GO:0044237), and translation (GO:0006412). With respect to the co-expressed mitochondrion CDS, the top GO terms showing very high enrichments included: organonitrogen compound biosynthetic process (GO:1901566), translation (GO:0006412), amide biosynthetic process (GO:0043604), and peptide biosynthetic process (GO:0043043). Intrigued by these findings, we additionally performed the functional annotation of the co-expressing CDS using Blast2GO. The top terms obtained under the biological category for chloroplast and mitochondrion CDS have been shown in Supplementary Fig. 6. Further, ECs were obtained for the annotated sequences (Supplementary Fig. 6). Out of the six major EC classes, the co-expressed chloroplast CDS belonged to four EC classes, oxidoreductases, transferases, hydrolases, and lyases in the decreasing order. Likewise, for the mitochondrion CDS co-expressing with lncRNAs, the EC distribution pattern revealed enrichment for two classes, oxidoreductases and hydrolases. The pathways enrichment analysis indicated that lncRNAs co-expressing with chloroplast and mitochondrion CDS are potentially involved in seven (including ‘purine metabolism’, ‘oxidative phosphorylation’, ‘pyrimidine metabolism’, and ‘thiamine metabolism’) and three pathways (including ‘oxidative phosphorylation’, ‘purine metabolism’, and ‘thiamine metabolism’), respectively (Supplementary Table 3). Figure 6 shows the co-expression patterns of selected lncRNAs and corresponding chloroplast and mitochondrial coding sequences (CDS).
qRT-PCR Based Expression Analysis of lncRNAs
Of the highly expressing (FPKM > 100) tissue/developmental stage-specific lncRNAs, eight were randomly selected to validate their expression profile across different developmental stages of leaf, inflorescence, and berry tissues using quantitative real time polymerase chain reaction (qRT-PCR). We observed similar trends of expression across tissues as those seen using the RNA-seq data (Fig. 7). For instance, lncRNA TR339885 was more expressed in berry compared to the other two tissues during both qRT-PCR and RNA-seq data based analysis (Fig. 7C). Likewise for lncRNA TR40939, it was observed to be more expressed in the leaf tissue at the mature stage based on both the analyses (Fig. 7D).
Discussion
Diverse studies have been conducted at the genomic, transcriptomic, proteomic, and metabolomic levels to understand growth, development, and the complicated process of berry ripening in V. vinifera42,43,44,45. Consequently, huge volumes of data have also been generated by the grapevine research community, which need to be carefully mined to unleash the potential players of gene regulation46. Like the popularly studied short non-coding RNAs (such as miRNAs, siRNAs, and piRNAs), lncRNAs have emerged as important regulators of gene expression in mammals and have been extensively studied at the tissue and cell levels under normal and disease conditions8,9,10,11,12. However, lncRNA research in plants has only recently gained momentum. In this study, we used computational approach to identify lncRNAs in V. vinifera and investigated their potential role in regulation of plant development and other processes. The identified lncRNAs were found to express specifically/differentially across the tissues and developmental stages, and their expression patterns were in sync with that of certain mature mRNAs and TFs functionally annotated to be involved in developmental and other biological processes. Moreover, the interaction analyses of V. vinifera miRNAs with the predicted lncRNAs revealed the potential regulation of the targets of the former by the latter.
While compiling the findings of the current study, we came across a recent study by Harris et al. in which the authors had demonstrated that the transcriptome assembly of single V. vinifera cultivar is insufficient to completely annotate V. vinifera reference genome (based on Pinot Noir-derived PN40024)47. Interestingly, the authors re-annotated the reference genome based on de novo transcriptome assembly of another cultivar, Riesling (recovering about 60% of the Riesling transcriptome) and also identified 3210 putative lncRNAs in the cultivar. With their aim to define varietal differences in the plant at the transcriptome level, the authors have focused on cultivar-specific protein-coding RNAs and lncRNAs. However, in the present study, we harnessed grapevine transcriptomic data from three tissues and ten developmental stages to identify 56,441 putative lncRNAs and understand the importance of their existence in this fruit crop. With the primary focus on exploring as many as possible V. vinifera lncRNAs, we as well adopted the de novo approach of assembling the transcriptome; however, the assembly was based on RNA-seq data from at least three different cultivars, Summer Black, Thompson seedless, and Muscat blanc (Supplemenatry Table 4). The lncRNAs were analyzed to gain an overall picture of the several different ways in which these transcripts could regulate important biological processes in V. vinifera such as development.
Foremost, the identified lncRNAs were characterized in silico, and it was observed that these were distributed unevenly but densely across the 19 chromosomes. Similar trends of chromosomal distribution were observed for cucumber and tomato lncRNAs48,49 suggesting that these can be transcribed from anywhere in the genome. Further, based on digital expression estimation, it was observed that though their abundance varied from low to moderate levels, grapevine lncRNAs were significantly differentially expressed (P-value < = 0.001 and 4-fold change) across inflorescence, berry, and leaf tissues at distinct developmental stages. A maximum number of DE lncRNAs was identified in the reproductive tissues; most of them were inflorescence-specific. Interestingly, the maximum proportion of developmental stage-specific DE lncRNAs was determined in the mature leaf stage. Kim and Sung50 have previously highlighted the potential of spatially and temporarily differentially expressed lncRNAs in regulating important developmental programs in eukaryotes. Based on digital expression analysis in plants such as Gossypium arboretum (cotton), Cicer arietinum (chickpea), Fragaria vesca (woodland strawberry), and Morus notabilis (mulberry), tissue-specific lncRNAs have been reported to be involved in fiber development, flower development, fruit and flower development, and floral organ and root development, respectively27,28,29,51. The present study is the first attempt to identify tissue- and developmental stage-specific lncRNAs in grapevine.
We conducted co-expression analysis to assign a tentative function to the grapevine lncRNAs with respect to the mature mRNAs, and functions of more than 6,000 co-expressing lncRNAs could be deduced using this strategy. The analysis revealed that lncRNAs co-expressed with mature mRNAs that code for enzymes involved in different biosynthetic, primary, and secondary metabolic pathways; for instance, enzymes involved in berry ripening. Grapes are non-climacteric fruits and the initiation of ripening is not controlled by a master switch (like in the case of climacteric fruits where ethylene plays a central and definite role). It has been suggested that abscisic acid (ABA) promotes ripening along with interplay of other hormones52,53. Particularly, ABA levels tend to increase at veraison (EL-35) as genes involved in ABA biosynthesis are more expressed54. Genes encoding 9-cis-epoxy-carotenoid dioxygenase (NCED) and zeaxanthin epoxidase (ZEP), which are two crucial plastidial enzymes in ABA biosynthesis, have been reported to be up-regulated at the EL35 berry stage in grapevine45,55. Further, it has been observed that during the later stages of ripening (EL-36 onwards), ABA synthesis is not induced; however, ABA-regulated processes (such as signaling networks) are activated53,54. In sync with these studies, we observed the relative expression patterns of grapevine lncRNAs co-expressing with NCED and ZEP (Supplementary Fig. 7). Co-expressing lncRNAs were found to be up-regulated at the EL-35 stage of berry development. Further investigation of these lncRNAs can pave ways into deeper understanding of the mechanisms of berry ripening and can help to resolve the complications of transcriptional reprogramming in other non-climacteric fruits, as well.
Likewise, based on pathway enrichment analysis, grapevine lncRNAs were found to be associated with carotenoid biosynthesis. Interestingly, carotenoids (a subgroup of secondary metabolites- isoprenoids) can act as precursors for ABA, which further regulates plant development. Besides, it has been observed in an independent study that transcriptional regulation of carotenoid biosynthesis pathway genes controls carotenoid content during different developmental stages of fruit ripening56. Furthermore, grapevine lncRNAs were found to be associated with phenylpropanoid, flavonoid, and stilbenoid biosynthesis, indicating their potential as regulators of secondary metabolic pathways in the plant (Supplementary Fig. 8). Recently, efforts have been made in this direction by constructing integrated networks57. Wong and Matus identified new TFs, miRNAs, and one lncRNA that regulated the expression of stilbene synthase (SBS) genes. The present study highlights the potential of lncRNAs in regulation of several secondary metabolites in the plant (Supplementary Table 3). In future, experiments can also be directed towards understanding the underlying regulatory mechanisms of these metabolites.
TFs are instrumental players in regulatory networks. As modular proteins, they interact with both coding and non-coding genes, and are capable of controlling developmental transitions58. Such transitions require chromatin re-organization, and TFs in union with chromatin regulators modulate the gene expression during plant development. However, these interactions leading to repression and activation of target genes are not completely understood. Functional analyses of human lncRNAs (like XIST) have revealed that these transcripts can bind and mediate recruitment of chromatin-modifying complexes, thereby, acting in cis to regulate the target gene expression59,60,61. Likewise, in Arabidopsis, lncRNAs (like COLDAIR) have been identified that mediate epigenetic silencing of the target genes by directing chromatin modifications and regulate important developmental processes, such as flowering13,62. Such lncRNAs that act as scaffolds for chromatin-modifying complexes are not only involved in gene repression but have also been reported as enhancers of expression63,64. Thus, lncRNAs are potential links that can help us better understand the molecular dynamics of epigenetic and transcriptional reprogramming during plant development. We observed important families of TFs that co-expressed with grapevine lncRNAs across different developmental stages. Particularly, AP2/ERF superfamily was found to be highly enriched. The members of this family have been reported to be specifically differentially expressed in a study based on transcriptomic analysis of grape berry at different developmental stages31. Moreover, other highly co-expressed TFs observed by us, such as WRKY, MYB, bHLH, and Trihelix, were also reported to express differentially in young, veraison, late-veraison, and ripe berries. Different groups of VvWRKY family of genes have been reported to be expressed specifically in leaf tissues during senescence65, while some have been associated with pathogen defense in ripe berries as the tissue at that stage becomes more prone to fungal attacks31. These findings indicate the involvement of grapevine lncRNAs in common developmental and/or metabolic pathways as those associated with TFs in the plant.
In our study, not all the identified lncRNAs were found to co-express with the mRNAs as the former have been reported to function through diverse mechanisms. Moreover, developmental switches are controlled by several key regulators, for instance, TFs, miRNAs, chromatin regulators, etc.58; hence, further strategies were developed to mine as many as possible potential roles of grapevine lncRNAs, especially in regulating plant development. Therefore, we studied the interaction of lncRNAs with miRNAs and observed that lncRNAs are potential targets, precursors, and eTMs of the latter. Target mimicry, as an lncRNA-mediated miRNA regulatory mechanism, has been reported in plants like Arabidopsis16 and Populus66. We identified several putative eTMs, which can regulate TFs via sequestration/sponging of miRNAs. For instance, lncRNA ‘TR123921’ acts as an eTM for miRNA ‘vvi-miR156h’. The original target for this miRNA has been predicted as Squamosa Promoter Binding Protein (SBP), which are TFs known to be involved in inflorescence development and have also been recently reported in Chinese wild Vitis as regulators of floral transition. High expression levels of this eTM were observed in different stages of inflorescence as compared to the leaf and berry tissues. This reflects the lncRNA-mediated positive regulation of the target genes of miRNAs and their impact on plant development. The miRNA family 156 has been known to be associated with SPL TFs in other plants like A. thaliana, O. sativa, S. lycopersicum, and Z. mays and affect floral development and plant transition from juvenile to adult stage67,68,69,70,71.
Grapevine mitochondrial genome is one of the largest sequenced plant organelle genomes72, but the role of lncRNAs in regulation of the mitochondrial genes is unknown. We explored the potential role of lncRNAs in regulation of extra-chromosomal genes, that is, mitochondrial and chloroplast CDS and identified co-expressing lncRNAs that were associated with key biological processes such as photosynthesis, oxidative phosphorylation, and purine/pyrimidine metabolic pathways. Our findings support the feasibility of an additional layer in the regulation of extra-nuclear genes in plants. Further studies aiming at understanding the biogenesis and mechanisms of regulation of these lncRNAs can help solve the mysteries of plant mitogenome and plastome. Studies in mammals have shown that though nucleus is the site of biogenesis of several lncRNAs, these transcripts can dwell in the mitochondrion to facilitate the coordinated signaling system and regulate mitochondrial functions73,74.
In this study, we have observed the numerous ways through which lncRNAs are potentially involved in regulation of developmental transitions and other key processes in V. vinifera. Besides the in silico analyses, qRT-PCR results have highlighted the differential expression patterns of selected lncRNAs across different tissues and developmental stages. These experimentally validated lncRNAs will now further be studied to understand the molecular mechanisms of their action.
Conclusion
We used a computational pipeline to identify 56,441 putative lncRNAs in V. vinifera from RNA-seq data. Further, we identified DE lncRNAs across inflorescence, berry, and leaf tissues at different developmental stages; of which, some transcripts exhibited tissue- and developmental stage-specificity. We observed that 22% of the tissue-specific lncRNAs were highly expressed in the berry, particularly at the mature fruit and veraison stages. Moreover, mature leaf-specific lncRNAs were highly expressed, which draws our attention to explore the possible roles lncRNAs could play in vegetative tissues as well. Co-expression analysis-mediated functional annotation primarily revealed the association of grapevine lncRNAs with development, biosynthetic pathways, and secondary metabolic pathways. Interaction of lncRNAs with miRNAs as their putative precursors, targets, and endogenous target mimics enabled us to propose the plausible mechanism of action for the former. TFs have been known to regulate developmental transitions in grapevine; however, their underlying mechanisms of action are not fully understood. Co-expressing TFs and lncRNAs can be further studied to understand their interaction with chromatin modifiers in repressing and activating genes during different developmental stages of the plant. Lastly, our findings draw attention towards the potential of lncRNAs to regulate extra-nuclear genes in plants. Overall, our results highlight the importance of lncRNAs in coordinating developmental transitions and other biological processes in grapevine.
Methods
Transcriptomic Data Collection
RNA-seq data pertaining to different tissues such as leaf (young, medium- and large-sized, and mature), inflorescence (3, 5, and 7 days after 100% cap-fall), and berry (veraison, intermediate, and mature) of V. vinifera were obtained based on published studies75,76,77 using National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) (http://www.ncbi.nlm.nih.gov/sra). The details of the RNA-seq data collected from NCBI-SRA database have been included in Supplementary Table 5.
A Computational Approach for Identification of lncRNAs Using the Collected RNA-seq Data
A computational pipeline was developed to identify putative lncRNAs using Trinity package34 as shown in Fig. 8. After the final assembly, which included removal of low-quality reads and adapter-containing sequences, a six-frame translation was conducted for the resulting contigs, and those coding for proteins greater than 100 amino acids in length were excluded. Next, the coding potential of these putative non-coding transcripts was evaluated using Coding Potential Calculator (CPC)35, and only the transcripts with scores less than 0 were used for subsequent analysis. The remaining transcripts were searched against the NCBI non-redundant (NR) protein database by BLASTX to exclude transcripts with significant homology to the known proteins. The transcripts for which no hits were obtained were considered. In order to verify our prediction approach, we compared our putative lncRNAs to the already reported lncRNAs from CANTATAdb36 by performing standalone BLASTN.
In silico Characterization and Classification of the Putative lncRNAs
The chromosome sequences of V. vinifera were downloaded from NCBI Nucleotide database, and standalone BLASTN analysis was conducted for lncRNAs with the following parameters: per cent identity >90 and e-value < 1e-10. The results were compared to the data available for 37,420 coding sequences (CDS), that is, the mature mRNA transcripts of the plant. These CDS were also downloaded using NCBI resources. For classification of the identified lncRNAs based on their genomic locations relative to that of the neighboring protein-coding genes, Cuffcompare program bundled with Cufflinks package v2.2.178 was used. The classification was aided by the “class codes” generated in the output with respect to the reference protein-coding genes (see Supplementary Data 1). The GTF-formatted files used as input for this analysis are provided in the Supplementary Data 2 and 3.
Differential Expression Analysis
The collected RNA-seq data (as discussed above) from 3 tissues and 10 developmental stages were used to determine the expression levels of both the putative lncRNAs and mature mRNA transcripts. These were quantified as number of RNA-seq fragments per kilobase of transcript effective length per million fragments mapped to all transcripts (FPKM) using RNA-Seq by Expectation-Maximization (RSEM) software that was bundled with Trinity package v2.4.0. To study the DE transcripts across the samples, the Empirical analysis of Digital Gene Expression (edgeR) was invoked, and those DE transcripts that exhibited at least 4-fold change at P-values (false discovery rate [FDR]) < = 0.001 in any of the pair-wise sample comparisons were selected. The expression profiles of lncRNAs and mature mRNAs were compared across the different samples with the aid of heat maps developed using Hierarchical Clustering Explorer v3.579 (http://www.cs.umd.edu/hcil/hce).
Tissue and Developmental Stage Specificity Estimation
To identify the lncRNAs that were specifically expressed in a given tissue and developmental stage, we calculated an index of tissue/developmental stage specificity for each transcript based on respective expression values estimated as FPKM. These indices were calculated based on the method described by Julien et al. and recently also applied by Shumayla et al.30,39. Briefly, the specificity index has been defined as the quotient of consensus expression value of a transcript and the sum of consensus expression values in all tissues and developmental stages. The resulting index ranges from 0 to 1 and corresponds to housekeeping and tissue-/developmental stage-specific lncRNAs accordingly. We used 0.7 as the threshold value of the index to estimate tissue and developmental stage specificity of the lncRNAs.
Functional Annotation of the Predicted lncRNAs
Prediction of lncRNA functions was carried out based on co-expression analysis of mature mRNAs and lncRNAs as reported previously in some studies30,80, that is, identification of the coding and non-coding transcripts co-expressing in different tissues and developmental stages. The expression data (in the form of FPKM) for both mature mRNAs and lncRNAs that were obtained as described above were fed into CoExpress v.1.5 tool, filtered by removing transcripts with the average expression <30, and analyzed following the instructions provided in the corresponding user manual81. The following parameters were applied: measure, Pearson correlation; correlation power, 1; filtering threshold, 0.9; and number of runs for bootstrapping, 100. Next, Blast2Go was used to perform gene ontology (GO) enrichment for the mRNAs co-expressing with lncRNAs40. Further, pathway enrichment analysis was conducted for the mRNAs co-expressing with lncRNAs to gain insight into the potential pathways being regulated by using Kyoto Encyclopedia of Genes and Genomes (KEGG)82 Pathways database.
Interaction of lncRNAs with Transcription Factors (TFs)
The CDS of the known TFs for V. vinifera available in the Plant TF database v4.083 (PlantTFDB) were downloaded, and co-expression analysis (as described above) was conducted to identify the co-expressing TF-lncRNA pairs with similar parameters.
Interaction of lncRNAs with Other RNAs
For understanding the interaction between the short ncRNAs (miRNAs) and lncRNAs, V. vinifera-specific 186 mature miRNAs were downloaded from miRNA database (miRBase). To predict the miRNA target sites in lncRNAs, plant small RNA target analysis server84 (psRNATarget) was used with default parameters.
Furthermore, in order to explore the role of lncRNAs as precursors of V. vinifera miRNAs, 163 stem-loop sequences of the latter were downloaded from miRBase. Standalone BLASTN was performed to compare the downloaded sequences with the lncRNAs. In order to identify the lncRNAs acting as eTMs, TAPIR85 (http://bioinformatics.psb.ugent.be/webtools/tapir/) was used with mfe_ratio >= 0.7.
The secondary structures based on minimum free energy were analyzed using Vienna RNAfold web server (http://rna.tbi.univie.ac.at/)86.
To understand the relationships among the coding and non-coding RNAs, interaction network analysis was conducted using Gephi (https://gephi.org/)87.
Potential Regulation of Extra-chromosomal Genetic Material by lncRNAs
The extra-chromosomal genome of V. vinifera, that is, nucleotide sequences of the chloroplast and mitochondrion were retrieved using the NCBI Nucleotide database, and standalone BLASTN analysis was conducted with respect to the lncRNAs. Further, using NCBI resources, 84 and 74 chloroplast and mitochondrial CDS were downloaded, and the DE analysis (as described above) was conducted to study their expression patterns across the different tissues and developmental stages. Next, co-expression analysis was conducted in the aforementioned manner to identify the co-expressing chloroplast- and mitochondrial-lncRNA pairs by using similar parameters.
qRT-PCR Based Expression Analysis of lncRNAs
To validate the expression of lncRNAs across different tissues, leaf (young- 2 weeks, medium- 5 weeks, large- 7 weeks, mature 10 weeks), inflorescence (3, 5, and 7 days after 100% cap-fall), and berry (veraison, intermediate, and mature) samples of V. vinifera cv. Thompson seedless were collected during the ongoing season in March-June 2018. Total RNA was isolated using the protocol by Ghawana et al.88, and the quantity and integrity of the RNA samples were analyzed by measuring 260/280 nm ratios using Nanodrop spectrophotometer and by 1.2% agarose gel electrophoresis, respectively. cDNA was prepared using Bio-Rad iScriptTM Select cDNA synthesis kit. The primers for qRT-PCR were designed using Primer3 Input software (Supplementary Table 6). PCR amplifications were carried out using Bio-Rad CFX96™ Real-Time PCR system. Elongation Factor-1 Alpha (EF1α) and actin7 (ACT7) (NCBI reference sequence ID: XM_002284888.3 and XM_002282480.4, respectively) were used as internal control genes for normalization of gene expression. 2−ΔΔCT method was used to estimate the relative gene expression89. All the experiments were conducted in triplicates.
References
Wang, K. C. & Chang, H. Y. Molecular mechanisms of long noncoding RNAs. Molecular cell 43(6), 904–914 (2011).
Kapranov, P. et al. RNA maps reveal new RNA classes and a possible function for pervasive transcription. Science 316(5830), 1484–1488 (2007).
Ponting, C. P., Oliver, P. L. & Reik, W. Evolution and functions of long noncoding RNAs. Cell 136(4), 629–641 (2009).
Struhl, K. Transcriptional noise and the fidelity of initiation by RNA polymerase II. Nature structural & molecular biology 14(2), 103–105 (2007).
Dieci, G., Fiorino, G., Castelnuovo, M., Teichmann, M. & Pagano, A. The expanding RNA polymerase III transcriptome. TRENDS in Genetics 23(12), 614–622 (2007).
Wierzbicki, A. T., Ream, T. S., Haag, J. R. & Pikaard, C. S. RNA polymerase V transcription guides ARGONAUTE4 to chromatin. Nature genetics 41(5), 630–634 (2009).
Böhmdorfer, G. et al. Long non-coding RNA produced by RNA polymerase V determines boundaries of heterochromatin. Elife 5, e19092 (2016).
Wu, R. et al. Characters, functions and clinical perspectives of long non-coding RNAs. Molecular genetics and genomics 291(3), 1013–1033 (2016).
Serviss, J. T., Johnsson, P. & Grandér, D. An emerging role for long non-coding RNAs in cancer metastasis. Frontiers in genetics, 5 (2014).
Ishii, N. et al. Identification of a novel non-coding RNA, MIAT, that confers risk of myocardial infarction. Journal of human genetics 51(12), 1087–1099 (2006).
Stuhlmüller, B. et al. Detection of oncofetal h19 RNA in rheumatoid arthritis synovial tissue. The American journal of pathology 163(3), 901–911 (2003).
Tsoi, L. C. et al. Analysis of long non-coding RNAs highlights tissue-specific expression patterns and epigenetic profiles in normal and psoriatic skin. Genome biology 16(1), 24 (2015).
Swiezewski, S., Liu, F., Magusin, A. & Dean, C. Cold-induced silencing by long antisense transcripts of an Arabidopsis Polycomb target. Nature 462(7274), 799–802 (2009).
Heo, J. B. & Sung, S. Vernalization-mediated epigenetic silencing by a long intronic noncoding RNA. Science 331(6013), 76–79 (2011).
Ding, J. et al. A long noncoding RNA regulates photoperiod-sensitive male sterility, an essential component of hybrid rice. Proceedings of the National Academy of Sciences 109(7), 2654–2659 (2012).
Franco-Zorrilla, J. M. et al. Target mimicry provides a new mechanism for regulation of microRNA activity. Nature genetics 39(8), 1033–1037 (2007).
Yang, W. C. et al. Characterization of GmENOD40, a gene showing novel patterns of cell‐specific expression during soybean nodule development. The Plant Journal 3(4), 573–585 (1993).
Bardou, F. et al. Long noncoding RNA modulates alternative splicing regulators in Arabidopsis. Developmental cell 30(2), 166–176 (2014).
Ariel, F. et al. Noncoding transcription by alternative RNA polymerases dynamically regulates an auxin-driven chromatin loop. Molecular cell 55(3), 383–396 (2014).
Shi, W., Quan, M., Du, Q. & Zhang, D. The Interactions between the Long Non-coding RNA NERDL and Its Target Gene Affect Wood Formation in Populus tomentosa. Frontiers in plant science 8, 1035 (2017).
Liu, X., Hao, L., Li, D., Zhu, L. & Hu, S. Long non-coding RNAs and their biological roles in plants. Genomics, proteomics & bioinformatics 13(3), 137–147 (2015).
Nejat, N. & Mantri, N. Emerging roles of long non-coding RNAs in plant response to biotic and abiotic stresses. Critical Reviews in Biotechnology, 1–13 (2017).
Bhatia, G., Goyal, N., Sharma, S., Upadhyay, S. K. & Singh, K. Present Scenario of Long Non-Coding RNAs in Plants. Non-Coding RNA 3(2), 16 (2017).
Rinn, J. L. et al. Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs. Cell 129(7), 1311–1323 (2007).
Umlauf, D. et al. Imprinting along the Kcnq1 domain on mouse chromosome 7 involves repressive histone methylation and recruitment of Polycomb group complexes. Nature genetics 36(12), 1296–1300 (2004).
Zhang, Y. C. et al. Genome-wide screening and functional analysis identify a large number of long noncoding RNAs involved in the sexual reproduction of rice. Genome biology 15(12), 512 (2014).
Zou, C. et al. Transcriptome analysis reveals long noncoding RNAs involved in fiber development in cotton (Gossypium arboreum). Science China. Life Sciences 59(2), 164 (2016).
Kang, C. & Liu, Z. Global identification and analysis of long non-coding RNAs in diploid strawberry Fragaria vesca during flower and fruit development. BMC genomics 16(1), 815 (2015).
Khemka, N., Singh, V. K., Garg, R. & Jain, M. Genome-wide analysis of long intergenic non-coding RNAs in chickpea and their potential role in flower development. Scientific reports 6, 33297 (2016).
Shumayla et al. Survey of High Throughput RNA-Seq Data Reveals Potential Roles for lncRNAs during Development and Stress Response in Bread Wheat. Front. Plant Sci. 8, 1019, https://doi.org/10.3389/fpls.2017.01019 (2017).
Sweetman, C., Wong, D. C., Ford, C. M. & Drew, D. P. Transcriptome analysis at four developmental stages of grape berry (Vitis vinifera cv. Shiraz) provides insights into regulated and coordinated gene expression. BMC genomics 13(1), 691 (2012).
Wang, L., Sun, X., Weiszmann, J. & Weckwerth, W. System-level and granger network analysis of integrated proteomic and metabolomic dynamics identifies key points of grape berry development at the interface of primary and secondary metabolism. Frontiers in plant science 8, 1066 (2017).
Shangguan, L. et al. RNA-Sequencing Reveals Biological Networks during Table Grapevine (‘Fujiminori’) Fruit Development. PloS one 12(1), e0170571 (2017).
Haas, B. J. et al. De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nature protocols 8(8), 1494–1512 (2013).
Kong, L. et al. CPC: assess the protein-coding potential of transcripts using sequence features and support vector machine. Nucleic acids research 35(suppl_2), W345–W349 (2007).
Szcześniak, M. W., Rosikiewicz, W. & Makałowska, I. CANTATAdb: a collection of plant long non-coding RNAs. Plant and Cell Physiology 57(1), e8–e8 (2015).
Li, B. & Dewey, C. N. RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinformatics 12, 323, https://doi.org/10.1186/1471-2105-12-323 (2011).
Robinson, M. D., McCarthy, D. J. & Smyth, G. K. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26, 139, https://doi.org/10.1093/bioinformatics/btp616 (2010).
Julien, P. et al. Mechanisms and evolutionary patterns of mammalian and avian dosage compensation. PLoS biology 10(5), e1001328 (2012).
Conesa, A. & Götz, S. Blast2GO: A comprehensive suite for functional analysis in plant genomics. International journal of plant genomics, 2008 (2008).
Griffiths-Jones, S., Saini, H., van Dongen, S. & Enright, A. miRBase: tools for microRNA genomics. Nucleic Acids Research 36, 154 (2007).
Grimplet, J. et al. Comparative analysis of grapevine whole-genome gene predictions, functional annotation, categorization and integration of the predicted gene sequences. BMC Research notes 5(1), 213 (2012).
Ghan, R. et al. The common transcriptional subnetworks of the grape berry skin in the late stages of ripening. BMC plant biology 17(1), 94 (2017).
Rattanakan, S., George, I., Haynes, P. A. & Cramer, G. R. Relative quantification of phosphoproteomic changes in grapevine (Vitis vinifera L.) leaves in response to abscisic acid. Horticulture research 3, 16029 (2016).
Deluc, L. G. et al. Transcriptomic and metabolite analyses of Cabernet Sauvignon grape berry development. BMC genomics 8(1), 429 (2007).
Adam-Blondon, A. F. et al. Towards an open grapevine information system. Horticulture research 3, 16056 (2016).
Harris, Z. N., Kovacs, L. G. & Londo, J. P. RNA-seq-based genome annotation and identification of long-noncoding RNAs in the grapevine cultivar ‘Riesling’. BMC genomics 18(1), 937 (2017).
Hao, Z. et al. Genome-wide identification, characterization and evolutionary analysis of long intergenic noncoding RNAs in cucumber. PloS one 10(3), e0121800 (2015).
Wang, J. et al. Genome-wide analysis of tomato long non-coding RNAs and identification as endogenous target mimic for microRNA in response to TYLCV infection. Scientific reports 5, 16946 (2015).
Kim, E. D. & Sung, S. Long noncoding RNA: unveiling hidden layer of gene regulatory networks. Trends in plant science 17(1), 16–21 (2012).
Song, X. et al. Genome-wide identification and characterization of long non-coding RNAs from mulberry (Morus notabilis) RNA-seq Data. Genes 7(3), 11 (2016).
McAtee, P., Karim, S., Schaffer, R. & David, K. A dynamic interplay between phytohormones is required for fruit development, maturation, and ripening. Frontiers in Plant Science, 4 (2013).
Pilati, S. et al. Abscisic acid is a major regulator of grape berry ripening onset: new insights into ABA signaling network. Frontiers in plant science 8, 1093 (2017).
Fortes, A. M., Teixeira, R. T. & Agudelo-Romero, P. Complex interplay of hormonal signals during grape berry ripening. Molecules 20(5), 9326–9343 (2015).
Fortes, A. M. et al. Transcript and metabolite analysis in Trincadeira cultivar reveals novel information regarding the dynamics of grape ripening. BMC plant biology 11(1), 149 (2011).
Leng, X. et al. Genome-wide identification and characterization of genes involved in carotenoid metabolic in three stages of grapevine fruit development. Scientific Reports 7(1), 4216 (2017).
Wong, D. C. & Matus, J. T. Constructing Integrated Networks for Identifying New Secondary Metabolic Pathway Regulators in Grapevine: Recent Applications and Future Opportunities. Frontiers in plant science, 8 (2017).
Kaufmann, K., Pajoro, A. & Angenent, G. C. Regulation of transcription in plants: mechanisms controlling developmental switches. Nature Reviews Genetics 11(12), 830–842 (2010).
Lee, J. T. The X as model forRNA’s niche in epigenomic regulation. Cold Spring Harbor perspectives in biology 2(9), a003749 (2010).
Khalil, A. M. et al. Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression. Proceedings of the National Academy of Sciences 106(28), 11667–11672 (2009).
Tsai, M. C. et al. Long noncoding RNA as modular scaffold of histone modification complexes. Science 329(5992), 689–693 (2010).
Liu, F., Marquardt, S., Lister, C., Swiezewski, S. & Dean, C. Targeted 3′ processing of antisense transcripts triggers Arabidopsis FLC chromatin silencing. Science 327(5961), 94–97 (2010).
Wang, K. C. et al. A long noncoding RNA maintains active chromatin to coordinate homeotic gene expression. Nature 472(7341), 120–124 (2011).
Ørom, U. A., Derrien, T., Guigo, R. & Shiekhattar, R. Long noncoding RNAs as enhancers of gene expression. In Cold Spring Harbor symposia on quantitative biology 75, 325–331. Cold Spring Harbor Laboratory Press (2010).
Wang, M. et al. Genome and transcriptome analysis of the grapevine (Vitis vinifera L.) WRKY gene family. Horticulture Research 1, 14016 (2014).
Shuai, P. et al. Genome-wide identification and functional prediction of novel and drought-responsive lincRNAs in Populus trichocarpa. Journal of experimental botany 65(17), 4975–4983 (2014).
Yamaguchi, A. & Abe, M. Regulation of reproductive development by non-coding RNA in Arabidopsis: to flower or not to flower. Journal of plant research 125(6), 693–704 (2012).
Huijser, P. & Schmid, M. The control of developmental phase transitions in plants. Development 138(19), 4117–4129 (2011).
Xie, K., Wu, C. & Xiong, L. Genomic organization, differential expression, and interaction of SQUAMOSA promoter-binding-like transcription factors and microRNA156 in rice. Plant physiology 142(1), 280–293 (2006).
Zhang, X. et al. Over‐expression of sly‐miR156a in tomato results in multiple vegetative and reproductive trait alterations and partial phenocopy of the sft mutant. FEBS letters 585(2), 435–439 (2011).
Chuck, G., Meeley, R., Irish, E., Sakai, H. & Hake, S. The maize tasselseed4 microRNA controls sex determination and meristem cell fate by targeting Tasselseed6/indeterminate spikelet1. Nature genetics 39(12), 1517–1521 (2007).
Goremykin, V. V., Salamini, F., Velasco, R. & Viola, R. Mitochondrial DNA of Vitis vinifera and the issue of rampant horizontal gene transfer. Molecular Biology and Evolution 26(1), 99–110 (2008).
Noh, J. H. et al. HuR and GRSF1 modulate the nuclear export and mitochondrial localization of the lncRNA RMRP. Genes & development 30(10), 1224–1239 (2016).
Dong, Y., Yoshitomi, T., Hu, J. F. & Cui, J. Long noncoding RNAs coordinate functions between mitochondria and the nucleus. Epigenetics & chromatin 10(1), 41 (2017).
Wen, Y. Q. et al. Using the combined analysis of transcripts and metabolites to propose key genes for differential terpene accumulation across two regions. BMC plant biology 15(1), 240 (2015).
Pervaiz, T. et al. Transcriptomic Analysis of Grapevine (cv. Summer Black) Leaf, Using the Illumina Platform. PloS one 11(1), e0147369 (2016).
Domingos, S. et al. Shared and divergent pathways for flower abscission are triggered by gibberellic acid and carbon starvation in seedless Vitis vinifera L. BMC plant biology 16(1), 38 (2016).
Trapnell, C. et al. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nature Biotechnology 28(5), 511 (2010).
Seo, J. & Shneiderman, B. A rank-by-feature framework for unsupervised multidimensional data exploration using low dimensional projections. In Information Visualization, 2004. INFOVIS 2004. IEEE Symposium, 65–72 (2004).
Liao, Q. et al. Large-scale prediction of long non-coding RNA functions in a coding–non-coding gene co-expression network. Nucleic acids research 39(9), 3864–3878 (2011).
Nazarov, P. V. et al. Interplay of microRNAs, transcription factors and target genes: linking dynamic expression changes to function. Nucleic acids research 41(5), 2817–2831 (2013).
Kanehisa, M., Furumichi, M., Tanabe, M., Sato, Y. & Morishima, K. KEGG: new perspectives on genomes, pathways, diseases and drugs. Nucleic Acids Research 45, D353–D361 (2017).
Jin, J. P. et al. PlantTFDB 4.0: toward a central hub for transcription factors and regulatory interactions in plants. Nucleic Acids Research 45(D1), D1040 (2017).
Dai, X. & Zhao, P. X. psRNATarget: a plant small RNA target analysis server. Nucleic Acids Research 39(suppl_2), W155 (2011).
Bonnet, E., He, Y., Billiau, K. & Van de Peer, Y. TAPIR, a web server for the prediction of plant microRNA targets, including target mimics. Bioinformatics 26(12), 1566–1568 (2010).
Gruber, A. R., Bernhart, S. H. & Lorenz, R. The ViennaRNA web services. RNA bioinformatics, 307–326 (2015).
Bastian, M., Heymann, S. & Jacomy, M. Gephi: an open source software for exploring and manipulating networks. Icwsm 8, 361–362 (2009).
Ghawana, S. et al. An RNA isolation system for plant tissues rich in secondary metabolites. BMC Res Notes. 4(1), 85 (2011).
Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25(4), 402–408 (2001).
Acknowledgements
Ms Garima Bhatia is thankful to ICMR, India for providing junior research Fellowship.
Author information
Authors and Affiliations
Contributions
K.S. and S.K.U. conceived the idea and designed the experiments. G.B. performed the experiments. G.B. and S.S. analyzed the data. K.S., S.S. and S.K.U. analyzed the results and wrote the manuscript. G.B. and K.S. finalized the manuscript.
Corresponding author
Ethics declarations
Competing Interests
The authors declare no competing interests.
Additional information
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Bhatia, G., Sharma, S., Upadhyay, S.K. et al. Long Non-coding RNAs Coordinate Developmental Transitions and Other Key Biological Processes in Grapevine. Sci Rep 9, 3552 (2019). https://doi.org/10.1038/s41598-019-38989-7
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-019-38989-7
- Springer Nature Limited
This article is cited by
-
Single-cell transcriptional landscape of long non-coding RNAs orchestrating mouse heart development
Cell Death & Disease (2023)
-
Transcriptome-guided annotation and functional classification of long non-coding RNAs in Arabidopsis thaliana
Scientific Reports (2022)
-
Genome-wide identification and characterization of long noncoding RNAs during peach (Prunus persica) fruit development and ripening
Scientific Reports (2022)
-
Investigation of long non-coding RNAs as regulatory players of grapevine response to powdery and downy mildew infection
BMC Plant Biology (2021)