Abstract
The spontaneous activity of sinoatrial node (SAN) pacemaker cells is generated by a functional interplay between the activity of ionic currents of the plasma membrane and intracellular Ca2+ dynamics. The molecular correlate of a dihydropyridine (DHP)-sensitive sustained inward Na+ current (I st), a key player in SAN automaticity, is still unknown. Here we show that I st and the L-type Ca2+ current (I Ca,L) share CaV1.3 as a common molecular determinant. Patch-clamp recordings of mouse SAN cells showed that I st is activated in the diastolic depolarization range, and displays Na+ permeability and minimal inactivation and sensitivity to I Ca,L activators and blockers. Both CaV1.3-mediated I Ca,L and I st were abolished in CaV1.3-deficient (CaV1.3−/−) SAN cells but the CaV1.2-mediated I Ca,L current component was preserved. In SAN cells isolated from mice expressing DHP-insensitive CaV1.2 channels (CaV1.2DHP−/−), I st and CaV1.3-mediated I Ca,L displayed overlapping sensitivity and concentration–response relationships to the DHP blocker nifedipine. Consistent with the hypothesis that CaV1.3 rather than CaV1.2 underlies I st, a considerable fraction of I Ca,L was resistant to nifedipine inhibition in CaV1.2DHP−/− SAN cells. These findings identify CaV1.3 channels as essential molecular components of the voltage-dependent, DHP-sensitive I st Na+ current in the SAN.
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Introduction
Heart automaticity is generated by the spontaneous excitation of sinoatrial node (SAN) pacemaker cells. Spontaneous activity is due to the presence of the diastolic depolarization, which leads the membrane voltage from the end of the repolarization phase to the threshold of the following action potential. There has long been considerable debate regarding the ionic mechanisms underlying diastolic depolarization, reflecting the complex nature of this physiological process. Diastolic depolarization requires a net inward current, which results from the relative balance between the decaying outward delayed rectifier K+ currents (I Kr and I Ks) and the growing inward currents (see Mangoni and Nargeot for review1). Previous studies have identified several voltage-gated inward currents activated in the diastolic depolarization range, including the hyperpolarization-activated inward current (I f)2, the L- and T-type Ca2+ currents (I Ca,L and I Ca,T)3, 4 and the sustained inward Na+ current (I st)5. Additionally, recent experimental evidence has supported an alternative mechanism to promote pacemaker activity, in which spontaneous local Ca2+ release from intracellular Ca2+ stores stimulates electrogenic Na+–Ca2+ exchanger (I NCX) activity to depolarize the membrane voltage during diastolic depolarization6. Thus, multiple inward current systems rather than a single pacemaker current are responsible for the spontaneous activity in the SAN.
Selective pharmacological block or genetic ablation of ion channels has been extensively used to describe the contribution of ionic currents to pacemaker activity. The molecular correlates of most cardiac ionic currents have been identified allowing the development of genetically modified mouse models targeting specific ion channels including HCN4-7, 8, HCN2-I f 9, 10, CaV1.3-I Ca,L 11, 12, CaV3.1-I Ca,T 13 and Ncx1-I NCX 14. By contrast, the complete lack of knowledge about the molecular determinants of I st has so far prevented the evaluation of the physiological role of this important ionic current.
I st was reported as a novel inward current in SAN cells of several mammalian species including rabbits, guinea-pigs, rats and mice5, 15,16,17,18. I st is activated at low membrane voltages and supplies persistent inward current flowing over the full diastolic depolarization range. Therefore, it has been proposed that this current is a physiologically important contributor to diastolic depolarization19. However, two decades after the first description of I st by Guo et al.5, there remains little progress in the identification of the molecular determinant of I st. Furthermore, no specific blocker for I st is available, limiting the understanding of its physiological role in SAN pacemaker activity. Although I st is carried by Na+, its pharmacological features closely resemble those of I Ca,L: I st is not affected by the voltage-gated Na+ current (I Na) blocker tetrodotoxin (TTX), but is inhibited by various chemical classes of organic Ca2+ channel blockers, e.g. dihydropyridines (DHPs)5, and enhanced by the I Ca,L channel activator Bay-K864415. Moreover, like I Ca,L, I st is also stimulated by β-adrenergic activation5, 18. These pharmacological properties are highly specific for I Ca,L and suggest the possibility that the pore-forming α1-subunit of L-type Ca2+ channels which carry the drug-binding domains for organic Ca2+-channel blockers and activators20, 21 are also essential for I st activity. Cardiac L-type Ca2+ channels are heteromultimers in which the pore-forming α1 subunit associates with auxiliary subunits (in particular β, α2/δ subunits)22. In SAN cells, two different α1-subunits, CaV1.2 (α1C) and CaV1.3 (α1D), are expressed. CaV1.2 is uniformly expressed in heart tissue, whereas CaV1.3 is nearly absent in ventricles but is abundant in the conduction system including the SAN12, 23, 24. While CaV1.3 channels activate in the diastolic depolarization range, CaV1.2 channels are activated in the upstroke phase of the action potential12. In addition to forming distinct types of I Ca,L with different voltage dependencies of activation and inactivation, CaV1.3 and CaV1.2 channels are also differentially localized in SAN cell membranes25.
Here we tested the hypothesis that CaV1.2 and/or CaV1.3 L-type channels are required for generating I st. Using two genetically modified mouse strains we demonstrate that the CaV1.3 L-type Ca2+-channel isoform is essential for functional expression of I st in mouse SAN cells. Although the exact molecular mechanism linking CaV1.3 activity to I st remains to be elucidated, our data show that CaV1.3 channels participate in the formation of a DHP-sensitive, voltage-dependent Na+ conductance in SAN cells.
Results
Identification of I st in mouse SAN cells
The magnitude of I st varies depending on SAN cell types with distinct morphologies5. Mouse SAN cells used for I st recordings were typically spindle- or spider-shaped with no obvious striations. These cells were small (C m, 34.8 ± 1.2 pF, n = 42) compared to rod-shaped atrial-like cells and were spontaneously beating when superfused with normal Tyrode solution. To confirm the presence of I st in these cells, the late currents elicited by 1-s depolarizing voltage-clamp steps to various test potentials from a holding potential of −90 mV were examined for the characteristics of I st (Fig. 1). In order to avoid contamination of recordings by K+ currents, we employed a Cs+-rich internal solution. I f was removed by substituting K+ with Cs+ in the external Tyrode solution, which contained 1.8 mM Ca2+. To confirm the sensitivity of the sustained current to DHPs, the typical hallmark of I st 19, we tested the sensitivity of the current to the potent DHP L-type Ca2+-channel blocker isradipine, which has not previously been tested. In Fig. 1Aa, membrane currents recorded under control conditions (black trace), after lowering [Ca2+]o from 1.8 to 0.1 mM (blue trace) and during subsequent application of 1 µM isradipine (red trace) are superimposed at individual test potentials. In the control bathing solution, membrane depolarization positive to −60 mV evoked a large transient inward current attributable to the activation of I Na and I Ca,T (note that peaks are not to scale in the figure), followed by a late inward current sustained during the entire period of 1-s depolarizing pulses. An inward current with a slow current decay was observed at test potentials of >−40 mV, as expected for I Ca,L activation. The current-to-voltage (I–V) relationship obtained by plotting the current amplitude measured near the end of test pulses indicated that the late current level becomes more inward with increasing depolarization between −70 and −50 mV (black circles, Fig. 1Ab), generating a negative slope conductance in the range of the diastolic depolarization. Lowering external Ca2+ reduced a considerable fraction of I Ca,L at membrane voltages positive to −30 mV (inset, Fig. 1Aa), whereas the sustained inward current was not reduced. It is thus unlikely that the sustained inward current was generated by a window component of I Ca,L. However, bath application of isradipine readily inhibited the sustained inward current and unmasked an almost linear background conductance (Fig. 1Aa,b). Under conditions of low [Ca2+]o, the DHP-sensitive sustained inward current peaked at −50 mV and the current direction was reversed at ~+ 26 mV (Fig. 1Ac).
Since I st has been shown to be carried by Na+ 5, we tested the permeability of the sustained inward current component for Na+. The external Na+ was replaced with an equimolar amount of N-methyl-D-glucamine (NMDG) in the presence of 1.8 mM Ca2+ (Fig. 1B). Perfusion of SAN cells with Na+-free NMDG solution readily suppressed the sustained inward current as well as Na+-dependent background conductance (Fig. 1Ba and b). Subsequent application of 1 µM nifedipine did not affect the late inward current component, indicating that Na+ was the predominant ion carrying the DHP-sensitive sustained inward current. At voltages positive to −20 mV the outward current component was partially reduced by nifedipine (average current density of the DHP-sensitive outward current, 0.34 ± 0.08 pA/pF at + 20 mV; n = 4, two independent experiments: N = 2), suggesting that Cs+ was carrying the DHP-sensitive current component.
We next evaluated the kinetics of inactivation of the sustained inward current in mouse SAN cells in further detail (Fig. 1C and D). In the experiment shown in Fig. 1C, the inactivation time course was determined by measuring the fractional change of the sustained current elicited by a depolarizing step to −50 mV from a holding potential of −80 mV in 0.1 mM [Ca2+]o, immediately (0.05 s) before (I ref) and after (I test) conditioning pulses to 0 mV of variable duration (0.5–4.5 s). Nifedipine was then applied to acquire the background current (red trace) at −50 mV, which was used to evaluate the net amplitude of the DHP-sensitive inward current. In Fig. 1C the ratio of I test/I ref is plotted as a function of the conditioning pulse duration, indicating that while I st displayed slow inactivation (τ = 1.94 ± 0.57s, n = 3, N = 1), a considerable current fraction remained available even after a 4.5-s conditioning pulse (0.48 ± 0.04, n = 3, N = 1). In addition, recovery from inactivation was assessed by applying a 5-s conditioning prepulse followed by test pulses to −50 mV after varying intervals of recovery (0.05–8.05 s) at −80 mV (Fig. 1D). Recovery of the sustained current proceeded exponentially with a time constant of 2.66 ± 0.63s (n = 3, N = 1).
These properties (low voltage for activation, DHP sensitivity, Na+ permeability and slow inactivation), clearly identified the sustained inward current in our mouse SAN cell preparations as I st 5, 15,16,17,18, 26. We only failed to record the sustained current in five of 24 experiments (~20%), which is likely to indicate inhomogeneous expression of I st in SAN cells16. Four of five I st-deficient cells were nearly indistinguishable from clear striated atrial-like myocytes.
I Na and I NCX do not contribute to I st in mouse SAN
To assess whether voltage-gated Na+ currents could interfere with I st recordings in mouse SAN cells, we investigated the effect of the I Na blocker TTX on the membrane current (Fig. 2A). Since I st exhibited little inactivation during the 1-s square pulse, the I–V relationship was measured using a slow (150 mV/s) voltage-ramp protocol in 0.1 mM [Ca2+]o. Under these conditions, the contributions of I Ca,L and I Ca,T to the total membrane current were minimized5, 15, 18. Figure 2Aa shows a superimposition of the original current traces in response to the voltage ramp in the control (black trace), during 10 µM TTX application (blue trace) and after nifedipine application (red trace). Figure 2Ab displays the corresponding I–V relationships obtained from the descending limb of the voltage ramp. As evidenced in the current recordings and in the corresponding I–V curve, bath application of TTX readily inhibited the transient inward I Na at the beginning of the pulse (see expanded traces in the inset), but did not affect the subsequent current. Application of nifedipine (1 µM) then revealed that the TTX-insensitive and DHP-sensitive current component could be attributed to I st (Fig. 2Ac). It should be noted that partial inhibition of the late inward current by TTX was observed in six of 23 cells (26%) (average current density of TTX-sensitive current, 0.82 ± 0.17 pA/pF at −50 mV; n = 6, N = 4), suggesting that some mouse SAN cells also express a TTX-sensitive persistent Na+ current27.
The involvement of I NCX was also investigated (Fig. 2B). I st was hardly affected by total replacement of external Na+ with an equimolar concentration of Li+ to abolish I NCX. This result is consistent with the previously characterized selectivity of I st to monovalent cations26. In conclusion, I NCX did not contaminate our recordings of I st.
Sensitivity of I st to I Ca,L modulators in mouse SAN cells
We then characterized the pharmacological properties of I st by testing its sensitivity to various I Ca,L modulators in the presence of 0.1 mM [Ca2+]o solution. In the experiment shown in Fig. 2C, 0.1 µM isoprenaline (Iso) strongly increased I st (116.1 ± 16.8%, n = 8, N = 2, p = 0.0003). This stimulatory effect was almost reversed by addition of 1 µM acetylcholine (ACh) in the presence of Iso (84.3 ± 2.7%, n = 4, N = 2, p = 0.0029). Finally, the non-DHP I Ca,L blocker diltiazem (1 µM) completely abolished I st. By contrast, the I Ca,L agonist FPL-64176 (1 µM) potentiated the amplitude of I st nearly twofold, whereas application of 1 µM verapamil totally abolished I st (Fig. 2D). Our observations, in addition to the findings of previous studies5, 15, 18, indicate that the pharmacological properties of I st are undistinguishable from those of I Ca,L (Table 1).
I st is absent in CaV1.3−/− SAN cells
The undistinguishable pharmacological properties of I st and I Ca,L (Table 1) provided a strong rationale for testing the hypothesis that these currents share common molecular determinants. It is now generally accepted that I Ca,L in SAN cells is composed of two separate current components mediated by distinct pore-forming alpha subunits, CaV1.2 and CaV1.311, 12. To directly examine the possibility of a functional link between I st and CaV1.3, we recorded I Ca,L and I st in SAN cells from mice lacking CaV1.3 channels (CaV1.3−/− mice, Fig. 3). Since most SAN cells obtained from CaV1.3−/− mice were quiescent, we selected single cells for recordings based on morphological criteria rather than spontaneous activity. After the control recording in Cs+-substituted, K+-free Tyrode solution with 1.8 mM [Ca2+]o (black traces), I st was separated from I Ca,L by switching the bath solution to 0.1 mM [Ca2+]o containing 10 µM TTX (blue traces). I st was identified as a current component inhibited by subsequent application of 1 µM nifedipine (red traces). Consistent with previous studies, genetic ablation of CaV1.3 channels resulted in considerable reduction of I Ca,L 12 as well as a shift in the current half-activation voltage11, 12 (Fig. 3A–C). Indeed, the peak density of I Ca,L was significantly reduced from −6.97 ± 0.85 pA/pF in wild-type SAN cells (n = 19, N = 6) to −4.81 ± 0.45 pA/pF in CaV1.3−/− cells (n = 18, N = 6, p = 0.0336), and was accompanied by a positive shift in the peak of the I–V relationship by ~20 mV (Fig. 3C). The calculated half-maximal activation voltage (V 0.5act) was shifted from −29.3 mV in wild-type cells to −12.8 mV in CaV1.3−/− SAN cells.
I st was evident in wild-type SAN cells after I Ca,L removal by lowering [Ca2+]o, as manifested by the increase in the sustained inward current with depolarization between −70 and −50 mV that was finally blocked by nifedipine (Fig. 3A and B). By contrast, the late current obtained from CaV1.3−/− SAN cells changed linearly with command voltage in the 0.1 mM [Ca2+]o solution with 10 µM TTX, and there remained no detectable DHP-sensitive current. As shown in Fig. 3D, the average peak density of I st was −0.98 ± 0.09 pA/pF (n = 19, N = 6) in wild-type cells, while it was reduced below detectable levels in CaV1.3−/− cells (n = 18, N = 6). Thus, we concluded that I st was virtually absent in SAN cells from CaV1.3−/− mice.
The above results suggested that CaV1.3 mediated two different currents in SAN cells, i.e. Ca2+-conducting I Ca,L and Na+-conducting I st. To support this hypothesis and estimate the contribution of CaV1.3-mediated I Ca,L and I st to the diastolic depolarization, I Ca,L and I st were alternately recorded in the same cell under distinct external ionic conditions and activation in the diastolic depolarization range was evaluated in wild-type and CaV1.3−/− SAN cells (Fig. 4). A slow ascending ramp (−65 to −35 mV, 100 mV/s) voltage command was employed to mimic the diastolic depolarization. We first recorded I Ca,L using the 0 Na+, 1.8 mM Ca2+ external solution (Fig. 4A). Under these recording conditions, the voltage ramp gradually activated an inward current yielding negative slope conductance in wild-type SAN cells. This current was strongly augmented by Iso and inhibited by subsequent application of nifedipine. The nifedipine-sensitive difference current showed that the net I Ca,L started to activate clearly within the diastolic depolarization range, as expected for the low-voltage activation of CaV1.3-mediated I Ca,L. The average threshold for activation of CaV1.3-mediated I Ca,L was −51.2 ± 1.0 mV under control conditions and −59.8 ± 0.9 mV upon perfusion of Iso (n = 7, N = 3). Iso significantly augmented the amount of charge carried by CaV1.3-mediated I Ca,L from 0.068 ± 0.013 to 0.177 ± 0.023 pQ/pF (n = 7, N = 3, p = 0.0003). In contrast to wild-type SAN cells, significant nifedipine-sensitive Cav1.3-mediated I Ca,L was not recorded in Cav1.3−/− SAN cells (Fig. 4B). Similar to wild-type cells, CaV1.2-mediated I Ca,L could be elicited by subsequent depolarization at +10 mV. We did not find a statistically significant difference in the response of I Ca,L at +10 mV to Iso between wild-type (112.0 ± 8.7%, n = 7, N = 3) and CaV1.3−/− SAN cells (94.6 ± 6.1%, n = 6, N = 3, p = 0.1319). Taken together, these observations indicated that CaV1.3 channels alone fully accounted for I Ca,L in the pacemaker potential range.
We then switched to an external recording solution containing 140 mM Na+, 0.1 mM Ca2+ and 10 µM TTX to record I st in the same cells. As illustrated in Fig. 4E, slow replacement of the bathing solution enabled the monitoring of gradual changes in membrane currents. These changes included a marked inward shift in the holding current and a reduction in I Ca,L at +10 mV (indicated by arrows). In contrast to I Ca,L, we observed an increase in the inward current accompanied by a negative shift in the peak potential, which indicated that increased I st offsets the loss of I Ca,L along the voltage ramp. Similar to I Ca,L, I st was enhanced by Iso and blocked by nifedipine (Fig. 4C). I st was detected in all wild-type SAN cells (0.228 ± 0.039 and 0.453 ± 0.076 pQ/pF in the absence and presence of Iso, respectively, n = 7, N = 3). However, we failed to record I st in CaV1.3−/− SAN cells (0.012 ± 0.002 and 0.014 ± 0.004 pQ/pF in the absence and presence of Iso, respectively, n = 6, N = 3, Fig. 4D).
We did not observe either I st or I Ca,L in atrial-like myocytes isolated from wild-type SAN (n = 4, N = 3, data not shown). Thus, the presence of I st was always coupled to the low voltage-activated I Ca,L, which is consistent with the view that CaV1.3 mediates both I Ca,L and I st.
CaV1.2 channels are not involved in the generation of I st
The absence of I st in CaV1.3−/− SAN cells does not exclude the possibility that CaV1.2 also contributes to the generation of I st. To examine the involvement of CaV1.2 in I st, we employed knock-in mice in which a point mutation (T1066Y) abolishes the sensitivity of CaV1.2 to DHPs without changing channel function and expression (CaV1.2DHP−/− mice, Fig. 5)28. In this mouse model, selective blockade of CaV1.3 by DHPs enables the functional contributions of CaV1.2 and CaV1.3 to the generation of I Ca,L to be distinguished. We first tested the effect of nifedipine on I Ca,L in SAN cells from CaV1.2DHP−/− mice (Fig. 5A). I Ca,L was recorded after elimination of I st and I Na by Na+ removal from the external recording solution. Bath application of nifedipine (0.03–1 µM) reduced the peak amplitude of I Ca,L in a concentration-dependent manner to a maximum of ~64% even at a saturating concentration of DHP (1 µM). This residual DHP-resistant I Ca,L was completely blocked by application of verapamil (3 µM), in line with previous data showing that the T1066Y mutation preserves the high sensitivity of CaV1.2 to phenylalkylamines29. Peak inward current of the nifedipine-resistant component activated more slowly (Fig. 5A), as expected for CaV1.2-mediated I Ca,L 30. The presence of this DHP-insensitive component is consistent with our earlier finding11, 12 that I Ca,L in SAN cells is mediated by both CaV1.2 and CaV1.3.
We then examined whether altered sensitivity of CaV1.2 to DHP also affected the response of I st to nifedipine. The concentration-dependent inhibition of I st by nifedipine was investigated in SAN cells isolated from wild-type and CaV1.2DHP−/− mice (Fig. 5B). After suppressing I Ca,L by lowering [Ca2+]o to 0.1 mM, I st was elicited by the voltage ramp in the presence of nifedipine at various concentrations (0.03–1 µM). I st in CaV1.2DHP−/− SAN cells was reduced by nifedipine to levels similar to those observed in wild-type cells. Indeed, we failed to detect a nifedipine-resistant I st component at 1 µM. Fitting the concentration–response relationship to the Hill equation gave a half-maximal inhibitory concentration (IC50) value of 118.5 ± 28.8 nM (n = 4, N = 4) in CaV1.2DHP−/− SAN cells, similar to the value of 129.1 ± 28.8 nM (n = 4, N = 4) in wild-type cells. Thus, it is unlikely that CaV1.2 confers the structural basis for I st sensitivity to DHPs. Of interest, the IC50 for I st was close to that for I Ca,L in CaV1.2DHP−/− SAN cells (104.2 ± 26.6 nM, n = 4, N = 4, Fig. 5A), suggesting that CaV1.3 is responsible for the DHP sensitivity of I st.
Discussion
Here we have demonstrated, for the first time, that voltage-gated L-type CaV1.3 Ca2+ channels are essential for the expression of a DHP-sensitive, voltage-dependent Na+ conductance, previously described as I st. Our finding is based on the observations that (1) I st is consistently identified in wild-type SAN cells but not in CaV1.3-deficient cells; (2) block of I st by nifedipine was unaffected upon ablation of CaV1.2 DHP sensitivity in CaV1.2DHP−/− SAN cells; (3) DHP sensitivity of I st overlapped that of Cav1.3-mediated I Ca,L in CaV1.2DHP−/− SAN cells; and (4) I st could not be attributed to late I Na or I NCX. Sensitivity to Ca2+-channel blockers such as DHPs, verapamil and diltiazem, as well as activators such as Bay-K8644 and FPL-64176, is based on highly specialized structural motifs conserved in CaV1 α1-subunits of L-type Ca2+ channels21, 31. Thus, our genetic and pharmacological evidence showing overlapping properties between I st and CaV1.3-mediated I Ca,L indicates a close functional relationship between these currents in SAN cells (Table 1). The demonstration of CaV1.3 α1-subunits as essential molecular determinants of a voltage-dependent DHP-sensitive Na+ conductance is a novel and unexpected finding and constitutes a fundamental step in elucidating the molecular nature of I st.
Our patch-clamp recordings clearly show that under physiological conditions I st is predominantly carried by Na+ rather than Ca2+ ions. Indeed, while lowering external Ca2+ did not affect I st, removal of extracellular Na+ abolished the current even in the presence of a physiological concentration of Ca2+ (Fig. 1). L-type Ca2+ channels are permeable to Na+ in the absence of extracellular divalent cations32, 33. However, L-type Ca2+ channels are highly selective for Ca2+ over Na+ with a permeation ratio (P Ca/P Na) of ~1000 under physiological conditions32. Therefore, Na+ influx through L-type Ca2+ channels is blocked by extracellular Ca2+ in the submicromolar range32, 33. It is thus unlikely that the “classical” permeation pathway of L-type Ca2+ channels mediates I st. Indeed, currently available recombinant CaV1.3 channels with canonical channel pore sequence are Ca2+-selective I Ca,L, with poor permeability to Na+ at least in the experimental solutions used for the I st recording in the present study (Toyoda et al., unpublished observation). Our results therefore suggest that CaV1.3 α1-subunits in the SAN cell not only form CaV1.3 L-type channels but also contribute to the formation of voltage-gated Na+ conductance through an unknown mechanism.
However, revealing the molecular mechanism allowing CaV1.3 α1-subunits to form I st is challenging. We favour the hypothesis that CaV1.3 α1-subunits themselves form the I st pore due to the observation that I st and CaV1.3 possess an essentially indistinguishable pharmacological profile and Ca2+-channel blockers have been shown to exert their pharmacological modulation exclusively by binding to α1-subunits34. In this case the different ion selectivity of I st in SAN cells would require a modification of the ion permeation pathway. Substitution of negatively charged residues forming the ion selectivity filter of voltage-gated Ca2+ channels by lysine can indeed induce persistent Na+ currents similar to I st 35, 36, suggesting that increased Na+ permeability per se could reproduce I st properties. To date, analysis of CaV1.3 transcripts has not identified alternatively spliced CaV1.3 variants with a modified selectivity filter37,38,39. Since Na+ conductance through such modified channels may be larger than for Ca2+ 36, 40, I st transcripts may be present at low levels. This would make their detection particularly difficult in tissues with low cell numbers such as the SAN. On the other hand, the possibility that I st could be generated by alternative splicing of Ca2+ channels is also suggested by a recent report that T-type (CaV3) Ca2+ channels of the snail heart have high permeability to Na+ due to unique splicing in the outer pore region41. Although this possibility cannot be excluded for mammalian SAN, splicing of T-type α1-subunits appears an unlikely explanation for I st because of the L-type channel-specific pharmacology. Another possible explanation for Na+ selectivity of CaV1.3 α1-subunits could be structural modifications of the ion conducting pathway through RNA editing, which so far has only been detected in the brain and in the cytoplasmic C-terminal tail of the channel39, 42.
Alternatively, a cationic channel functionally coupled to CaV1.3 activity could mediate I st. In mouse SAN cells CaV1.3 is co-localized with sarcoplasmic reticulum ryanodine receptors (RyRs) and controls diastolic RyR-dependent Ca2+ release25, 43. RyR-dependent Ca2+ release could then activate an inward Na+ current. However, the possibility that I st is mediated by Ca2+-dependent opening of a cationic channel appears unlikely, because I st density did not decrease upon lowering extracellular Ca2+ (Fig. 1), as one would expect for Ca2+-dependent opening of a Na+-selective channel associated with CaV1.3. Finally, the possibility that I st could be generated by direct opening of a Na+ channel physically coupled to CaV1.3 channel gating is also unlikely, because I st activates negative to CaV1.3-mediated I Ca,L (Fig. 1).
CaV1.3 loss-of-function in mice or humans results in SAN dysfunction, which indicates that CaV1.3 channels play a major role in pacemaker activity11, 12, 44,45,46. Consequently, the present findings also suggest that the loss of I st could contribute to the SAN dysfunction induced by CaV1.3 gene inactivation. In addition, our results indicate that the heart rate reducing effect of Ca2+ channel antagonists can be explained by drug binding to CaV1.3 channels and reduction of CaV1.3-mediated I Ca,L and I st. Consistent with previous observations11, 12, our recordings in CaV1.3−/− SAN cells show that CaV1.3 underlies a low-threshold I Ca,L activated at voltages spanning the diastolic depolarization range. I st differs from CaV1.3-mediated I Ca,L in the charge carrier and shows a more negative voltage for half-activation. I st and CaV1.3-mediated I Ca,L could thus differentially contribute to the generation of the diastolic depolarization. For example, I st could generate a persistent Na+ influx in the diastolic depolarization, while CaV1.3-mediated I Ca,L could generate inward Ca2+ current12 and control RyR-dependent Ca2+ release43. Notably, both I st and CaV1.3-mediated I Ca,L are strongly potentiated by β-adrenergic activation, which suggests a dual role of CaV1.3 in the sympathetic control of heart rate via I Ca,L and I st.
In conclusion, we provide novel evidence supporting the involvement of CaV1.3 in the generation of I st in SAN cells. Our work provides valuable new insights into the molecular basis of I st as well as the diverse functional significance of CaV1.3 in cardiac pacemaker activity.
Methods
Ethics
The investigation conforms to the Guide for the Care and Use of Laboratory Animals (8th edition, 2011), published by the US National Institutes of Health and European directives (2010/63/EU). The experimental protocol was approved by the Institutional Animal Care and Use Committee of Shiga University of Medical Science (Nos 2009-5-11, 2012-1-10 and 2014-12-3), the University of Montpellier and the University of Innsbruck.
CaV1.3−/− and CaV1.2DHP−/− mice
CaV1.3−/− and CaV1.2DHP−/− mice were obtained by crossing mice from the original mutant colonies28, 44 with mice with a C57B6/J genetic background from Charles River in the animal facility, free of specific pathogenic organisms, of the Réseau d’Animalèrie de Montpellier (RAM) at the Institut de Génetique Humaine (Montpellier, France). We next backcrossed the offspring for 10 generations with C57B6/J mice before starting the study. Animals were given ad libitum access to food and drinking water and were maintained in a 12-h light–dark cycle (light, 8:30 a.m. to 8:30 p.m.). Only homozygous CaV1.3−/− and CaV1.2DHP−/− mice were used for the experiments.
SAN cell preparations
Isolation of single SAN cells from mouse hearts was performed according to the methods of Mangoni and Nargeot47. Wild-type (N = 18), CaV1.3−/− (N = 10) and CaV1.2DHP−/− (N = 5) mice were anaesthetized with ketamine (100 mg/kg) combined with xylazine (10 mg/kg), and anticoagulated with heparin (250 units/mouse). Beating hearts were quickly removed and the SAN region was excised and cut into small strips in warm (35 °C) Tyrode solution containing (in mM): 140.0 NaCl, 5.4 KCl, 1.8 CaCl2, 1.0 MgCl2, 5.0 HEPES-NaOH and 5.5 D-glucose (adjusted to pH 7.4 with NaOH). The SAN tissue strips were then transferred to a low-Ca2+, low-Mg2+ solution containing (in mM): 140.0 NaCl, 5.4 KCl, 0.5 MgCl2, 0.2 CaCl2, 1.2 KH2PO4, 50.0 taurine, 5.5 D-glucose and 5.0 HEPES-NaOH with 1.0 mg/ml bovine serum albumin (BSA) (adjusted to pH 6.9 with NaOH), and then subjected to digestion by adding Liberase TH (0.1 mg/ml, Roche Diagnostics GmBH) and elastase (1.9 U/ml, Worthington Biochem. Co.) at 35 °C for a variable time of 9–14 min. Tissue strips were then transferred and washed in a Kraft-Bruhe (KB) solution containing (in mM): 70.0 L-glutamic acid, 20.0 KCl, 80.0 KOH, 10.0 (±) D-β-OH-butyric acid, 10.0 KH2PO4, 10.0 taurine and 10.0 HEPES-KOH, with 1 mg/ml BSA (pH adjusted to 7.4 with KOH). SAN cells were manually dissociated by agitation using a flame-forged Pasteur pipette in KB solution at 35 °C for ~5 min. Cellular automaticity was recovered by readapting the cells to physiological extracellular Na+ and Ca2+ concentrations by adding aliquots of solutions containing (in mM): 10.0 NaCl, 1.8 CaCl2 and, subsequently, normal Tyrode solution containing 1 mg/ml BSA. The final storage solution contained (in mM): 100.0 NaCl, 35.0 KCl, 1.3 CaCl2, 0.7 MgCl2, 14.0 L-glutamic acid, 2.0 (±) D-β-OH-butyric acid, 2.0 KH2PO4 and 2.0 taurine, with 1.0 mg/ml BSA (pH 7.4). Cells were harvested in custom-made recording Plexiglass chambers with glass bottoms for proper cell attachment and rinsed with normal Tyrode solution warmed to 36 °C just before patch-clamp recording.
Whole-cell patch-clamp technique and data analysis
Isolated SAN cells were voltage-clamped using the whole-cell configuration of the patch-clamp technique with an EPC-8 patch-clamp amplifier equipped with an LIH-1600 AD/DA interface (HEKA) controlled by PatchMaster software or an Axon MultiClamp 700 A amplifier equipped with Digidata 1332 A interface-controlled PClamp software. Patch electrodes had a resistance of 2.5–4.0 MΩ when filled with the Cs+-rich intracellular solution containing (in mM): 125 CsOH, 20 tetraethylammonium chloride (TEA-Cl), 1.2 CaCl2, 5 Mg-ATP, 0.1 Li2-GTP, 5.0 EGTA and 10.0 HEPES (pH adjusted to 7.2 with aspartate). The concentration of free Ca2+ in the pipette solutions was calculated to be approximately 4.8×10−8 M (pCa = 7.3). The Cs+-substituted, K+-free external Tyrode solution contained (in mM): 140.0 NaCl, 5.4 CsCl, 1.8 CaCl2, 0.5 MgCl2, 0.33 NaH2PO4, 5.5 glucose and 5.0 HEPES (pH adjusted to 7.4 with NaOH). The concentration of CaCl2 in the external solution was reduced from 1.8 to 0.1 mM to separate I st from I Ca,L. In some experiments, NaCl was totally substituted with NMDG-Cl, TEA-Cl or LiCl. All experiments were performed at 34–36 °C.
Chemicals
Isradipine, nifedipine, verapamil, diltiazem, FPL-64176, Iso and ACh were purchased from Sigma-Aldrich. Drugs were prepared as 10 mM stock solutions in DMSO and then diluted in the external solution. TTX (Wako Chemical Co.) was dissolved in distilled water at a concentration of 10 mM and then diluted to the final concentration of 10 µM in the experimental solution.
Statistical analysis
The results are expressed as mean ± S.E.M. Statistical comparison among the different groups was performed by one-way ANOVA followed by Tukey’s post-hoc HSD test. Statistical comparison between two groups was evaluated using Student’s t-test. N indicates the number of hearts and n indicates the number of cells used in experiments. A p value < 0.05 was considered statistically significant.
Data availability
The datasets generated and/or analysed during the current study are available from the corresponding author upon request.
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Acknowledgements
The project was supported by the Grant-in-Aid for Scientific Research (C) 23590258, 26460295 and 17K08537 from the Japan Society for the Promotion of Science (to F.T.), the Agence Nationale pour la Recherche grants ANR-2010-BLAN-1128-01 and ANR-13-BSV1-023 (to M.E.M.) and the Austrian Science Fund (FWF, P27809). The IGF group is a member of the Laboratory of Excellence “Ion Channel Science and Therapeutics” (ICST) supported by a grant from ANR (ANR-11-LABX-0015). The authors are indebted to Isabelle Bidaud and to the staff of the RAM animal facility of Montpellier for technical assistance and management of the mouse lines.
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The experiments presented in this study were performed at the Shiga University of Medical Science and the Institut de Génomique Fonctionnelle, CNRS, UMR-5203, Inserm U 1191 in Montpellier. Specific contributions are as follows: conception and design of experiments: F.T., M.E.M. and H.M.; collection, analysis and interpretation of data: F.T., P.M., S.D., W.-G.D., M.E.M. and H.M.; drafting the manuscript and revising it critically for important intellectual content: F.T., J.S., M.E.M. and H.M. All authors critically revised the manuscript for technical and important contents.
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Toyoda, F., Mesirca, P., Dubel, S. et al. CaV1.3 L-type Ca2+ channel contributes to the heartbeat by generating a dihydropyridine-sensitive persistent Na+ current. Sci Rep 7, 7869 (2017). https://doi.org/10.1038/s41598-017-08191-8
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DOI: https://doi.org/10.1038/s41598-017-08191-8
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