Abstract
Soil-transmitted helminths (STHs) are major pathogens infecting over a billion people. There are few classes of anthelmintics and there is an urgent need for new drugs. Many STHs use an unusual form of anaerobic metabolism to survive the hypoxic conditions of the host gut. This requires rhodoquinone (RQ), a quinone electron carrier. RQ is not made or used by vertebrate hosts making it an excellent therapeutic target. Here we screen 480 structural families of natural products to find compounds that kill Caenorhabditis elegans specifically when they require RQ-dependent metabolism. We identify several classes of compounds including a family of species-selective inhibitors of mitochondrial respiratory complex I. These identified complex I inhibitors have a benzimidazole core and we determine key structural requirements for activity by screening 1,280 related compounds. Finally, we show several of these compounds kill adult STHs. We suggest these species-selective complex I inhibitors are potential anthelmintics.
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Introduction
Soil-transmitted helminths (STHs) are major pathogens of humans and livestock1,2. Over a billion humans are infected by STHs including roundworm (Ascaris lumbricoides), hookworm (Necator americanus and Ancylostoma duodenale), and whipworm (Trichuris trichiura) and helminth infections are responsible for multiple neglected tropical diseases (NTDs). These infections result in malnutrition, malaise and weakness, and can cause developmental defects and impaired growth in children3. In addition, STHs infect a high proportion of livestock leading to reduced yield. This is a particular problem in poorer communities where such losses can have major health and economic consequences. While there are excellent frontline anthelmintics including benzimidazoles (e.g., albendazole and mebendazole)4,5 and macrocyclic lactones (e.g., ivermectin)6,7, there are few classes of commercial anthelmintics and resistance to these drugs is widespread in livestock and cases of reduced efficacy have been observed in human parasites as well8,9. There is thus an urgent need for new classes of anthelmintic drugs to control and treat these major pathogens which present a key challenge in global health.
Any effective anthelmintic must target the STH without harming the vertebrate host. One way to do this is to develop drugs that target a process that is essential for the STHs but absent from the host and that is the approach we take here. We focus on a unique aspect of STH metabolism—rhodoquinone-dependent metabolism10,11,12,13. During the stages of the lifecycle when the STH lives in the soil outside the host, it generates energy using oxidative phosphorylation. Electrons enter the mitochondrial electron transport chain (ETC) either at complex I or via a number of quinone-coupled dehydrogenases that include complex II, succinate dehydrogenase. They are first transferred to ubiquinone (UQ) and then pass through complex III and complex IV where they ultimately are accepted by molecular oxygen as the terminal electron acceptor. As the electrons flow through the ETC, protons are translocated across the mitochondrial membrane, establishing a proton motive force which powers ATP synthesis by the F0F1-ATP synthase, complex V (Fig. 1a). This UQ-using oxidative ETC is identical between host and parasite in aerobic conditions. However, many STHs must survive extended periods in the highly anaerobic environment of the host gut—adult Ascaris, for example lives for months in these conditions. Without available oxygen as the terminal electron acceptor, the STHs cannot use the aerobic UQ-coupled ETC. However, STHs have a key adaptation that still allows them to use a rewired form of the ETC and it is this that provides the potential target for anthelmintics. Electrons still enter the ETC from NADH into complex I and onto the quinone pool but instead of flowing through to complex IV, they exit the ETC at complex II which now acts as a fumarate reductase rather than a succinate dehydrogenase (Fig. 1b). This allows fumarate to be used as a terminal electron acceptor. Crucially, UQ cannot be used as an electron carrier to power the fumarate reductase activity—instead STHs use rhodoquinone (RQ), a highly related quinone14,15,16. This basic pathway (often also termed ‘malate dismutation’) was first described over 50 years ago in a series of elegant biochemical studies (reviewed in refs. 10,11,17). More recently, the pathway for RQ synthesis and the key molecular switch that determines whether UQ or RQ is made were identified in the genetically tractable nematode C. elegans18,19,20 and thus we now know the way the electron transport chain is rewired, how the key electron carrier RQ is made, and many of the genes required.
From the perspective of anthelmintic discovery, RQ synthesis and RQ-dependent metabolism is a very attractive target. RQ is only made and used by a small number of animal species: nematodes, platyhelminths, annelids, and molluscs. Since STHs make and use RQ but vertebrate hosts do not, RQ synthesis and RQ-dependent metabolism provide a critical target that differs between host and parasite. If we could identify drugs that specifically target RQ synthesis or RQ-dependent metabolism, they should kill the parasite without affecting the host—this is our goal.
The rewired ETC that is used for RQ-dependent metabolism has three clear attack points for drugs: complex I, the sole entry point for electrons and the sole source of proton pumping; complex II, the critical exit point for electrons; and the pathway for RQ synthesis (schematic in Fig. 1c). We previously showed that the free-living nematode C. elegans makes and uses RQ18 and that inhibitors of complexes I or II, or mutation of the RQ synthesis pathway (the three main attack points) all block RQ-dependent metabolism in C. elegans. C. elegans is thus an excellent model for RQ-dependent metabolism and it should be possible to carry out high throughput drug screens in vivo in C. elegans to identify additional inhibitors of RQ-dependent metabolism.
In this study, we screened a collection of natural products comprising 480 distinct structural classes of compounds as well as their derivatives21,22 for compounds that affect our C. elegans model of RQ-dependent metabolism—this covers the broad chemical space of 25,000 individual natural products. We identified multiple structural groups of potential RQ-dependent metabolism inhibitors and showed that several of these have highly specific effects on individual ETC complexes. These include a family of compounds with a benzimidazole core that we show can specifically and potently target complex I—this is an unexplored activity for benzimidazole anthelmintics. We screened 1,280 benzimidazoles and identified a small structurally-related subset that shows good species selectivity, inhibiting C. elegans complex I with >10-fold lower concentrations than bovine or murine complex I. Several of these also have no detectable effect on growth in normoxia. Finally, we show that several of these benzimidazole complex I inhibitors are active against Heligmosomoides polygyrus, a mouse STH—their effect is most potent in adult stages which contain mainly RQ and rely on RQ for survival in the host gut. We have thus identified a distinct family of species-selective complex I inhibitors that potently kill C. elegans and H. polygyrus under conditions where they require RQ-dependent metabolism for survival. These are potential anthelmintics that may similarly kill STHs in the host gut where they require RQ-dependent metabolism.
Results
A screen in C. elegans for natural products that inhibit RQ-dependent metabolism
C. elegans is highly related to STHs23 and C. elegans makes and uses RQ18,20. It is thus an excellent system for screens to identify compounds that specifically block RQ synthesis and RQ-dependent metabolism. We previously showed that C. elegans requires RQ synthesis and RQ-dependent metabolism to survive exposure to potassium cyanide (KCN) for 15 h18. This observation is the basis for a previously published image-based movement assay for RQ-dependent metabolism. In outline, we treat C. elegans L1 larvae with 200 µM KCN for 15 h—wild-type worms that can produce RQ are immobile but alive and if we remove the KCN by dilution they rapidly recover movement (Fig. 1d). However, worms that cannot make RQ (e.g., that have mutations in the kynurenine pathway (Fig. 1d)) or that cannot carry out RQ-dependent metabolism (e.g., due to inhibition of complex I or complex II) are dead after 15-h KCN treatment and hence no recovery of movement is seen (Fig. 1e). This was first shown in Del Borrello et al.18 and we confirm those results here. This allows a simple drug screen for products that specifically kill C. elegans when they require RQ-dependent metabolism for survival. Worms are treated with 200 µM KCN with either test compounds or DMSO controls and KCN is removed after 15 h, and movement measured 3 h later. If worms recover movement similar to controls, then the compound does not affect RQ-dependent metabolism. However, if worms are dead after 15-h KCN treatment in the presence of the test compound, no recovery is seen—that compound is thus a potential inhibitor of RQ-dependent metabolism. Finally, to ensure that the compound’s effect is specific for RQ-dependent metabolism and not some more general effect on worm viability, we also examine the effect of each compound on the growth and viability of worms in normoxia where they do not require RQ and do not use RQ-dependent metabolism. If a compound has little effect on growth and viability in normoxia but is strongly lethal in our RQ-dependent metabolism assay, this is a potential specific inhibitor of RQ synthesis or RQ-dependent metabolism. Our goal here is to identify such compounds and characterize their effects on RQ-dependent metabolism—they are anthelmintic candidates that act via inhibition of the anaerobic RQ-dependent metabolism that allows STHs to survive in the host gut.
We screened a library of 480 natural products and their derivatives from the RIKEN Natural Product Depository (RIKEN NPDepo)21,22 in our KCN-based assay for RQ-dependent metabolism and also in a more traditional C. elegans normoxic growth and viability assay. 80 of these natural products (Authentic library) were known molecules with previously characterized biological activities. The remaining 400 compounds (Pilot library) are a subset of a larger library of ~25,000 diverse natural product derivatives (NPDs)—each compound screened is thus a representative of a family of structurally-related NPDs. This allows a rapid initial primary screen covering the broad structural space of the entire natural product collection and, once hits are identified, structurally-related NPDs are then screened in secondary assays (schematic in Fig. 2a). This helps define the structural requirements for the biological effects of the primary hits as well as identifying any related compounds with greater potency. The overall results are shown in Fig. 2b. For both assays, each compound was screened in triplicate at 50 µM and the mean value was expressed as a modified z-score. Compounds with modified z-scores < −3 (i.e., >3X median absolution deviation (MAD)) were identified as hits; all data are shown in Table 1. We conduct this primary screen at a high test compound concentration of 50 µM to reduce false negatives—we note that C. elegans drug screens typically use high primary screen drug concentrations as the worm has powerful xenobiotic responses that often reduce drug accumulation24.
We identified 9 compounds that only affect normoxic growth. Five of these were natural products with known activities and included nocodazole, an inhibitor of microtubule polymerization25; staurosporine, a broad-spectrum kinase inhibitor26; camptothecin, a DNA topoisomerase inhibitor27; reveromycin A, an isoleucyl-tRNA synthetase inhibitor28; and aristolochic acid, a known mutagen29. These drugs all target core essential cellular components and the observed lethality is consistent with this. We also identified four uncharacterized compounds that inhibited C. elegans growth and viability. Several show no activity in HEK293 cells and these are potential anthelmintics. However, for the purposes of this study, we focused on the 8 compounds that showed little effect on growth in normoxia (z-scores > −3) but killed worms specifically when they were relying on RQ-dependent metabolism for survival. These are candidate RQ-dependent metabolism inhibitors and we refer to these as ‘RQ-dependent metabolism hits’.
As described above, the full natural product library contains two groups of compounds: the larger group (Pilot library) is of NPDs with no previous reported bioactivity, and a second smaller group (Authentic library) includes compounds that had previously been shown to be active in at least one previous screen. This latter group yielded three potent RQ-dependent metabolism hits, two of which are known inhibitors of oxidative phosphorylation. Niclosamide is a salicylanilide anthelmintic used to treat tapeworm infections30. Niclosamide acts as an ionophore and exerts its lethal effects by collapsing mitochondrial membrane potential31. We had previously found that other salicylanilide anthelmintics such as closantel are powerful inhibitors of RQ-dependent metabolism (Supplementary Fig. 1) and thus this hit is expected. The second hit, siccanin, has been reported to target complex II in fungi, bacteria, and mammals32, and complex II is one of the key attack points of RQ-dependent metabolism (see Fig. 1c schematic). Consistent with this previously reported activity, we found siccanin also potently inhibits C. elegans complex II in vitro and shows strong activity within our RQ-dependent metabolism assay similar to other known complex II inhibitors (Supplementary Fig. 2). Thus 2 RQ-dependent metabolism hits in the characterized NPD set, niclosamide and siccanin, had clear roles in electron transport and mitochondrial function validating the relevance of our RQ-dependent metabolism hits. We turned our attention to the previously uncharacterized natural products.
NPD screens identify a distinct class of benzimidazole complex I inhibitors
We identified 7 NPDs as RQ-dependent metabolism hits that had no previously described bioactivity. We screened (also at 50 μM) an additional 137 compounds from the RIKEN NPDepo that represented analogs and/or derivatives of these 7 RQ-dependent metabolism hits and identified 15 structurally related compounds that also inhibited C. elegans survival in KCN (Supplementary Fig. 3 and 4). Since complex I and complex II are key attack points for RQ-dependent metabolism, we first tested whether these NPDs directly affect the electron transport chain in vitro using a range of enzymatic assays in purified C. elegans mitochondria (see Supplementary Fig. 5). Several of the RQ-dependent metabolism hits affect more than one complex (e.g., anacardic acid is a potent inhibitor of complexes III and IV) and others have no consistent effect on any specific complex (see Table 2 for data). However, one structural group of RQ-dependent metabolism hits (Fig. 3a) showed highly specific inhibition of complex I (Table 2). These compounds are structurally related to papaverine, an alkaloid antispasmodic drug which has also been shown to be able to inhibit complex I33,34 and this group of compounds act as potent complex I inhibitors in our hands (Fig. 3b). Complex I is the sole entry point for electrons into the ETC during RQ-dependent metabolism and we previously showed that rotenone, a complex I inhibitor, can block RQ-dependent metabolism (Fig. 1e). Three of this cluster of NPDs are highly related to papaverine but the fourth NPD in the cluster, NPD8790, is structurally distinct and has a benzimidazole core instead of an isoquinoline core. We found that papaverine, and the other isoquinoline-based NPDs, have no significant species selectivity for complex I (Fig. 3c and Supplementary Table 1). NPD8790, however, shows marked species selectivity, inhibiting C. elegans complex I (IC50 = 2.4 µM) more potently than complex I from either bovine (IC50 = 27.2 µM) or murine heart mitochondria (IC50 = 28.8 µM)(Fig. 3d and Supplementary Table 1). In addition, we tested NPD8790 on mitochondria purified from several other nematode species (Caenorhabditis briggsae, Pristionchus pacificus and Phasmarhabditis hermaphrodita) as well as mitochondria purified from HEK293 human embryonic kidney cells and find the IC50 of NPD8790 against complex I is similar for all nematode species (C. briggsae, IC50 = 1.6 μM; P. pacificus, IC50 = 2.1 μM; P. hermaphrodita, IC50 = 1.1 μM) but human mitochondria have a greater IC50 value (IC50 > 75 μM), similar to that seen in bovine and murine mitochondria (Supplementary Fig. 6). This is promising for a potential anthelmintic: complex I is used by both host and STH and thus a molecule that selectively targets STH complex I would be able to kill STHs with minimal adverse effects on the host.
NPD8790 has a benzimidazole core and benzimidazoles are well characterized front line anthelmintics that include mebendazole and albendazole4,5. The mode of action of known benzimidazole anthelmintics is well established: they target the beta-tubulin BEN-14,35,36,37 and prevent microtubule polymerization. However, we found NPD8790 has a very different activity: inhibition of complex I. We therefore wanted to examine whether known benzimidazoles also affect complex I and whether NPD8790, the benzimidazole that we identified as a complex I inhibitor, also targets BEN-1. We first tested whether any of the commercial benzimidazoles (e.g., fenbendazole in Fig. 4a, b and others in Supplementary Fig. 7) have similar effects to NPD8790 in our RQ-dependent metabolism assay or in normoxia. We find that while NPD8790 kills worms potently when they require RQ-dependent metabolism for survival, commercial benzimidazoles have no effect in the same RQ-dependent metabolism assay. Conversely, commercially available benzimidazole anthelmintics potently kill C. elegans in normoxia (Fig. 4b and Supplementary Fig. 7) whereas NPD8790 has very little effect on C. elegans normoxic growth until very high concentrations (greater than 500 µM). These data suggest that commercial benzimidazoles and NPD8790 have a different mode of action. Consistent with this, we find that commercial benzimidazoles have no detectable effect on complex I activity when assayed on purified C. elegans mitochondria (Fig. 4c). These data suggest NPD8790 targets complex I but commercial benzimidazoles do not. We note that while some early studies suggested a possible role for benzimidazole anthelmintics in affecting the electron transport chain38,39,40, these compounds were all subsequently shown to act by inhibition of microtubule polymerization and in our hands none of these has any effect on complex I activity. We also note that different groups reported different electron transport chain targets and, in addition, some of these early studies also found that levamisole (a known nicotinic acetylcholine receptor agonist) affected the electron transport chain39, suggesting that these studies had a significant rate of false positives in their assays. We thus would use caution interpreting some of these early findings and suggest that our findings here are the first solid evidence of a benzimidazole compound that acts as a specific and potent inhibitor of complex I.
Our data suggest that NPD8790 specifically targets complex I in the electron transport chain but we wanted to determine if NPD8790 might also affect the beta tubulin BEN-1 which is the target of all commercial benzimidazoles4,35,36,37. We confirmed that commercially available benzimidazole anthelmintics potently kill C. elegans in normoxia (Fig. 4b and Supplementary Fig. 7) and that either deletion of ben-1 or introduction of ben-1 mutations known to cause benzimidazole resistance in parasites36,37 greatly reduce sensitivity as expected (Fig. 4b). However, we find that ben-1 mutation or loss has no effect on NPD8790 activity. The small effect on growth at high concentrations of NPD8790 is unaffected by ben-1 mutation and the inhibition of RQ-dependent metabolism by NPD8790 is not affected by mutations in ben-1 (Fig. 4b). Finally, we purified mitochondria from several C. elegans strains carrying ben-1 mutations and find that mutations in ben-1 have no effect on the ability of NPD8790 to inhibit complex I (Fig. 4d). We thus conclude that NPD8790 is a benzimidazole compound that has a distinct mode of action to all available benzimidazole anthelmintics. While commercial benzimidazole drugs target BEN-1 and not complex I, NPD8790 targets complex I and not BEN-1.
Structure-activity analysis identifies requirements for potent complex I inhibition by NPD8790-related benzimidazole compounds
We identified NPD8790 as a benzimidazole compound that acts as a species-selective complex I inhibitor. To see if we could identify related compounds with either higher potency or higher species selectivity, we screened 1,280 structurally related benzimidazole compounds. We first assayed each compound in our RQ-dependent metabolism assay at 50 μM to identify benzimidazole compounds that potentially shared similar bioactivity with NPD8790. A total of 84 benzimidazole compounds (6.6%) reduced motility by at least 75% in our RQ-dependent metabolism assay (Fig. 5a). 51 of these 84 compounds were reordered (the remainder were very costly or unavailable) to perform more comprehensive dose responses. Each of the 51 compounds were assayed for their effect on complex I activity in both C. elegans and bovine mitochondria (Fig. 5b, c), as well as their ability to affect C. elegans growth and RQ-dependent metabolism. Finally, we also assayed toxicity in HEK293 cells. These data are all shown in Supplementary Data File 1. Encouragingly, all 51 benzimidazole hit compounds inhibited C. elegans complex I in vitro, supporting our hypothesis that inhibition of complex I by NPD8790 and structural analogs is the mechanism by which these compounds affect RQ-dependent metabolism (Fig. 5b and Supplementary Data File 1).
To gain insight into the structural requirements for benzimidazole-based compounds to act as complex I inhibitors we first compared the properties of the active and inactive benzimidazole compounds from our screen. In brief, active compounds tended to have lower molecular weights (287 Da vs. 316 Da), fewer hydrogen bond donors and acceptors (3.4 vs. 5.4), higher computed octanol/water coefficients (logP; 4.0 vs. 3.3), and lower topological polar surface area (TPSA; 31.9 vs. 59.2) (Supplementary Fig. 8). Together, this suggested smaller, more hydrophobic benzimidazole compounds were more likely to be active in our C. elegans assays, likely reflecting that these properties are beneficial for chemical accumulation in nematodes41. We next examined the chemical structure of the 51 active compounds and found almost all have a benzimidazole core which is linked to an aromatic ring—compounds with other groups or structures show much lower activity and this ‘benzimidazole linked to an aromatic ring’ is the consistent feature of all potent compounds. The key differences between the active molecules are in the precise position of the linkage to the aromatic ring, the length of the linkage, and the substitution of the aromatic ring. Compounds where the aromatic ring is linked via the nitrogen at position 1 of the benzimidazole core tend to show more potent complex I inhibition than compounds with the linkage through position 2 (Fig. 5d). We also find a complex effect on the length of the linker between the benzimidazole and the aromatic group on potency: compounds with either a 1-atom linker or 3 or more atom linkers tend to be strong inhibitors but compounds with a 2-atom linker show much reduced potency (Fig. 5e).
We combined the structure-activity data in Fig. 5 to define 3 distinct structural classes of benzimidazole complex I inhibitors—we will refer to these as classes A-C (Fig. 5f). All of them have a benzimidazole core which is linked to an aromatic ring. The key differences between the subclasses are the position and the length of the linkage (Supplementary Data File 2). Class A compounds include NPD8790 and in this class the aromatic ring is linked via position 2 of the benzimidazole group via a short linkage (1-atom linkage). These compounds are good complex I inhibitors with IC50 values well below 10 µM. More importantly, Class A includes the compounds with the strongest species selectivity e.g., NPD8790 has a >10-fold differences in IC50 between C. elegans and bovine complex I (Fig. 5c). They have very little detectable effect on growth in normoxic conditions and NPD8790 also shows low toxicity against mammalian cells even at high concentrations (Supplementary Data File 2). Thus, these are good candidates as anthelmintics.
The other two classes both contain multiple compounds with substantially more potent complex I inhibition than Class A e.g., compound 5567167, a class B compound, has a 75-fold lower IC50 for C. elegans complex I than NPD8790 (Fig. 5g). These compounds all have the aromatic rings linked through the 1st position of the benzimidazole core with linkages of varying lengths—the differences between the two classes is in the length of the linkage between the aromatic group and the benzimidazole core. Class B comprises compounds with a 1-carbon linkage—these are very potent inhibitors with reasonable species selectivity. Class C comprises 5-carbon linkage compounds—these show excellent potency with good species selectivity. Additionally, representatives of both Class B (STK697993) and Class C (7732524) similarly inhibit complex I from several free-living Clade V nematode species (C. briggsae, P. pacificus, and P. hermaphrodita) (Supplementary Fig. 9). We note that Class B and C compounds are good anthelmintic candidates but that both sets show effects on growth in normoxia unlike Class A compounds. Although they also show a slightly reduced species selectivity, many Class B and C compounds still demonstrate low toxicity against mammalian cells. Taken together, these data suggest that Class A compounds may selectively affect worms when they rely on RQ-dependent metabolism, making them the best candidates for anthelmintics.
To support complex I inhibition as the mode of action for NPD8790 analogs in our RQ-dependent metabolism assay, we next examined the relationship between the in vitro and in vivo potencies of representative compounds from our three classes (A-C) of putative benzimidazole complex I inhibitors. For comparison, we included two known complex I inhibitors (rotenone and fenazaquin) and an existing benzimidazole anthelmintic (febendazole) with no previously reported activity against complex I. We found a strong positive correlation (Pearson’s correlation coefficient = 0.94) existed between the in vitro complex I IC50 and in vivo RQ-dependent metabolism LC50 values, supporting the argument that complex I inhibition underlies activity in our RQ-dependent metabolism assay (Fig. 6). We found similar moderate-strong correlations when we expanded our analysis to include all compounds of classes A-C for both RQ-dependent metabolism and the C. elegans development and viability assays (Supplementary Fig. 10), providing further evidence that complex I is the in vivo target of the NPD8790 family. While there were some differences between in vitro and in vivo activities, we suggest these likely arise because of differences in drug metabolism or uptake among analogs (e.g., cuticle permeability, drug metabolism, or efflux). Finally, to confirm the activity of the NPD8790 family was distinct from existing anthelmintic drugs, we tested the activity of several NPD8790 analogs against the viability of a panel of C. elegans anthelmintic-resistant strains (e.g., benzimidazoles35, macrocyclic lactones42, imidazothiazoles43,44, Cry proteins45, cyclooctadepsipeptides46, ethyl benzamides24, and amino-acetonitrile derivatives47). None of the existing anthelmintic-resistant strains showed any evidence of reduced susceptibility to the NPD8790 analogs, highlighting the distinct bioactivity of the NPD8790 family and their potential for tackling anthelmintic resistance (Supplementary Fig. 7c and Supplementary Table 2).
Benzimidazole complex I inhibitors affect H. polygyrus, a mouse soil-transmitted helminth
We identified a family of benzimidazole compounds that act as species-selective inhibitors of complex I. Several of these only kill C. elegans when they require RQ for their survival and thus have potential as anthelmintics since they may likewise kill STHs in the anaerobic conditions that they encounter in the host. To test their effect on STHs, we used H. polygyrus, a mouse STH parasite that has a gut-dwelling adult phase that is used widely as an STH model48,49. We tested 11 of our compounds covering representatives from Class A, B, and C structures on two life-cycle stages: L3 and adults. In STHs, there is a strong shift in UQ to RQ ratios between free living stages, like the L3s we test here, and adults that reside in low oxygen host niches. L3s contain high UQ and low RQ levels and thus rely on UQ-dependent aerobic metabolism. In adults the situation is reversed—they have high RQ levels and use anaerobic RQ-dependent metabolism. The switch from UQ to RQ synthesis is largely regulated by alternative splicing of the critical polyprenyltransferase coq-219. Adults typically express high levels of the coq-2e isoform that is required for RQ synthesis and consistent with this, H. polygyrus adults express mostly the coq-2e isoform (67%)50. This shift from high UQ to high RQ allows us to compare the effect of our compounds on parasite life stages that rely mostly on UQ aerobic metabolism (L3s) with those that rely mainly on RQ (adults). Note that during the normal life cycle, adults only live in anaerobic conditions. In the in vitro assays, however, they encounter a normoxic environment but importantly since the majority of their quinone is RQ, they still use RQ-dependent metabolism.
We find that none of our compounds showed significant activity on L3s in vitro. This weak effect on H. polygyrus when they are using UQ-dependent aerobic metabolism is consistent with what we find in C. elegans—all of our compounds are more potent when the animals rely on anaerobic metabolism. However, 5 compounds (NPD8790, STK951902, 7925515, 7925804, 7732524) have potent effects on adult viability after 24-h exposure (Fig. 7). These comprise 2/3 Class A and 3/3 Class C compounds tested. This suggests that the active compounds specifically affect H. polygyrus adults—this is the stage when they primarily synthesize and use RQ rather than UQ as an electron carrier. We also tested all 11 compounds on adults in anaerobic conditions using an anaerobic chamber. These conditions should partly mimic the low oxygen situation in the host and thus should prevent any residual UQ-dependent aerobic metabolism that might occur in adults, despite their low UQ levels. We note that we did not use anaerobic conditions in C. elegans since we could not fit our imaging equipment into an available hood, and did not use KCN for the H. polygyrus experiments since in vitro the H. polygyrus animals were unhealthy in KCN alone. We find that the anaerobic environment generally increases the activity of Class A, B, and C compounds (Supplementary Fig. 11). The effect is small, however, and we note that the adults are weakly affected by the anaerobic conditions alone as the controls show a small decrease in viability. We suggest that the limited additional effect of the anaerobic conditions is because H. polygyrus adults primarily make RQ and thus principally depend on RQ-dependent metabolism in both normoxic and anaerobic conditions. These in vitro experiments suggest that this family of benzimidazole compounds target Complex I in a species-selective manner and kill adult H. polygyrus when they rely on RQ as the electron carrier driving energy metabolism. We conclude that these compounds, or related derivatives, may have potential as anthelmintics.
Finally, we tested 4 compounds for anthelmintic effects in vivo. We chose the two most potent compounds from Classes A and C since these classes showed the strongest effects on adults in vitro and tested them at a single high 200 mg/kg dose in mice infected with H. polygyrus (see Methods for details). The mice showed no toxicity following treatment with our benzimidazole compounds, however, reductions in H. polygyrus worm burdens relative to untreated controls (~19–41% reduction) (Table 3) were observed for all mice treated with the 4 NPD8790 analogs. Although the effects of the NPD8790 analogs were weaker than that of existing commercial anthelmintics in vivo (for example in previous studies by the Keiser group, 90%, 99%, and 62% reductions in H. polygyrus worm burdens were observed after a single dose of either 100 mg/kg fenbendazole, 1 mg/kg moxidectin, or 1.25 mg/kg levamisole, respectively48,51), we note that strong in vivo anthelmintic activity is a high standard to achieve, especially from a single oral dose. Thus we believe that the detectable in vivo activity from this preliminary testing of the NPD8790 family is an encouraging starting point. Weaker in vivo effects could result from myriad causes from rapid metabolism of the compounds by the host or gut microbiota to rapid uptake and excretion by the host and thus low levels of uptake into the parasite. We hypothesize multiple doses or longer treatment time might result in a more potent response as an inhibitor of metabolism might take longer to have an effect than a paralytic like levamisole. We note that in vitro, the effects of our benzimidazole compounds on adult H. polygyrus were greater after 72 h of treatment than after only 24 h treatment (Fig. 7) which supports the idea that these may be slower-acting antiparasitic drugs. We also note that mebendazole, the front line anthelmintic for human STH infections shows relatively weak efficacy in the H. polygyrus murine model (63.5% reduction in worm burden for single 300 mg/kg dose)52, suggesting other STH species may similarly have increased susceptibility to the NPD8790 family. We suggest that medicinal chemistry studies will be needed to identify derivatives that increase the stability or delivery of these benzimidazole-based complex I inhibitors to the parasites in vivo.
Discussion
Soil-transmitted helminths (STHs) are major human pathogens. While there are excellent frontline anthelmintics including macrocyclic lactones and benzimidazoles, the number of classes of anthelmintics is very limited and resistance is increasing and is already very high for some livestock parasites. There is thus an urgent need for new classes of anthelmintic drugs. In this study we focused on using C. elegans to screen for drugs that specifically inhibit the unusual anaerobic metabolism that STHs like Ascaris and hookworm use to survive the low oxygen conditions in the host gut. This metabolism requires rhodoquinone (RQ), an electron carrier that is absent from the hosts. We screened a library of natural products and their derivatives (NPDs) that contained both previously characterized and uncharacterized compounds and identified multiple distinct structural families of compounds that kill C. elegans specifically when they are surviving using RQ-dependent metabolism. These are candidate anthelmintics since they may similarly kill STHs in vivo when they rely on RQ-dependent metabolism for survival in the gut.
During RQ-dependent metabolism, electrons enter the ETC solely through complex I and exit through complex II and consistent with this we had previously shown that known complex I inhibitors and complex II inhibitors are potent inhibitors of RQ-dependent metabolism. We identify both specific complex I and complex II inhibitors in our screens—a distinct set of benzimidazole compounds as complex I inhibitors and siccanin as a complex II inhibitor. The way these two sets of compounds affect RQ-dependent metabolism is consistent with our previous findings as these are expected to block electron entry or exit from the ETC. Other RQ-dependent metabolism hits are more enigmatic however. For example, in our hands anacardic acid is a potent inhibitor of complexes III and IV in purified mitochondria (Table 2) and it is unclear mechanistically how it might be acting or why it has such a strong effect on RQ-dependent metabolism but appears to have no detectable effect on metabolism in normoxia. Other RQ-dependent metabolism hits appear to have no effect on the ETC and must be affecting RQ-dependent metabolism in some other unknown manner, perhaps through targeting other required components of RQ-dependent metabolism such as AMPK53 or RQ synthesis itself. Thus while the mode of action of some of the RQ-dependent metabolism hits fits with what is known of electron flow in the ETC in RQ-dependent metabolism, others will require future studies to identify the targets and mode of action.
The main focus of our analysis was on a distinct, previously uncharacterized family of benzimidazole compounds that we identified as potent and specific inhibitors of mitochondrial respiratory complex I. Benzimidazoles like mebendazole and albendazole are well characterized anthelmintics that target BEN-1, a beta tubulin. The benzimidazole compounds we identify have a distinct mode of action: we showed that their activity is not affected by mutations in ben-1, and that known benzimidazole anthelmintics do not affect complex I activity. This confirms that the inhibition of complex I by this family of compounds is a distinct activity for benzimidazoles. We note that there is a single previous report of a compound with insecticidal activity and a benzimidazole core that is described as a putative complex I inhibitor (e.g., 1-(3,7-dimethyl-7-ethoxy-2-octenyl)−2-methylbenzimidazole)54. However, the structure is distinct to the hits we have identified here—instead of an aromatic group linked to the benzimidazole core, it has a long terpenoid chain. Notably, its inhibition of complex I has only been shown in mammalian mitochondria (e.g., rat liver, bovine heart) and has not been shown for any species of nematode54,55. Furthermore, no species selectivity has ever been demonstrated for this compound.
We identified the complex I inhibitors in C. elegans but also tested their efficacy on H. polygyrus, a mouse STH that has a gut-dwelling adult phase. The compounds show weaker effects on H. polygyrus larvae, which contain largely UQ and thus do not use RQ-dependent metabolism. In contrast, several of the compounds act as potent anthelmintics in vitro against adult H. polygyrus. H. polygyrus adults, like other STHs, show a shift in splicing of coq-2 consistent with a shift from UQ synthesis to RQ synthesis and containing mostly RQ and not UQ, and these findings are consistent with a model that these benzimidazole-based complex I inhibitors specifically kill nematodes when they rely on RQ-dependent metabolism. We tested a small number of the most potent compounds in vivo to determine their efficacy in clearing a murine H. polygyrus infection. All compounds reduced H. polygyrus worm burdens relative to controls, however, the effects were weaker than that observed for existing commercial anthelmintics and this could be due to a number of complex factors including rapid metabolism of the compounds by either host or host gut microbiota or low levels delivered to the parasite in vivo because of rapid uptake by the host. We delivered only a single dose in vivo but note that longer exposure to the drugs in vitro resulted in more potent killing (Fig. 7) suggesting a multidose regimen might improve in vivo efficacy. We anticipate that a phase of medicinal chemistry is needed to identify derivatives with higher in vivo efficacy and that similar lead optimization strategies have proved successful for almost all other anthelmintic compounds in the past, including the recently developed anthelmintic, monepantel47. Finally we note that while the murine H. polygyrus model is an excellent screening tool it is not a perfect one—for example the frontline human anthelmintic mebendazole shows relatively weak efficacy in a murine H. polygyrus model52. Additionally, given the large abundance of commercially available benzimidazole analogs and existing studies that have examined the bioavailability of benzimidazole anthelmintics, we are optimistic that improvements can be made to the in vivo activity of the NPD8790 family. We thus believe the NPD8790 family of complex I inhibitors merit further drug development and have potential to lead to new anthelmintics.
In summary, we screened a natural product library in C. elegans to identify compounds that killed C. elegans only under conditions when they require RQ-dependent metabolism to survive. We believe that this is a reasonable model for helminths which rely on RQ-dependent metabolism to survive in the highly anaerobic environment of a host gut. We identified compounds that specifically inhibit either complex I or complex II in a species-specific manner. Complex I and II are the entry and exit points for electrons in the RQ-coupled ETC and thus the mode of action is consistent with what is known of the ETC in RQ-dependent metabolism. The complex I inhibitors we identified are a family of compounds with a benzimidazole core, which represents a distinct activity for benzimidazoles—well characterized benzimidazole anthelmintics have no activity against complex I and target microtubules. We thus believe that, to the best of our knowledge, we have uncovered a unique class of benzimidazoles that specifically target the unusual anaerobic metabolism of helminths and that derivatives of these lead compounds may act as anthelmintics.
Methods
Ethical statement
All animal experimental procedures and husbandry in this study were performed by trained personnel in accordance with either the ethical committee of the canton Basel-Stadt (permission no. 520) and the University of Basel, or the Canadian Council on Animal Care with approval from the Animal Care Committee at the University of Toronto.
Chemical sources
Natural Product Libraries (Authentic & Pilot) and related derivatives used in preliminary screens were provided by the RIKEN Natural Product Depository (NPDepo). Identified hit compounds were purchased from Vitas-M for retesting. NPD8790 analogs were purchased from ChemBridge Corporation and Vitas-M. Wact-11 (6222549) was purchased from ChemBridge Corporation. Rotenone, antimycin A, potassium cyanide, albendazole, mebendazole, thiabendazole, and fenbendazole were purchased from MilliporeSigma. Siccanin was purchased from Cayman chemical. Chemical stock solutions were made for each compound by dissolving a weighed amount of chemical into a measured volume of sterile solvent (DMSO, ethanol, or water depending on compound solubility), stock solutions were then sterile filtered using 0.2-micron filters when appropriate. All stock solutions were stored at −20 °C prior to use apart from KCN, which was prepared fresh before each experiment.
Free-living nematode strains and maintenance
In addition to the Caenorhabditis elegans wild-type N2 strain, this work included the following C. elegans strains: CB1003 (kynu-1(e1003) X); anthelmintic-resistant strains—CB3474 (ben-1(e1880) III), CB193 (unc-29(e193) I), HY496 (bre-1(ye4) IV), RB2119 (acr-23(ok2804) V), JD608 (avr-14(ad1302) I; avr-15(ad1051) V; glc-1 (pk54) V), NM1968 (slo-1(js379) V), RP2635 (mev-1(tr355) III); benzimidazole-resistant CRISPR strains—ECA882 (ben-1(ean64) III), ECA917 (ben-1(ean98) III), ECA1075 (ben-1(ean143) III), ECA1080 (ben-1(ean148) III). This work also featured several additional free-living nematode strains: C. briggsae strain AF16, P. pacificus strain PS312, and P. hermaphrodita strain B178. The four CRISPR-generated benzimidazole-resistant C. elegans mutants were generously provided by Prof. Erik Andersen (Johns Hopkins University); PS312 and B178 were generously provided by Prof. Peter Roy (University of Toronto); all other strains were obtained from the Caenorhabditis Genetics Center (CGC, University of Minnesota). All strains were cultured and propagated at 20 °C on NGM (nematode growth medium) agar plates seeded with E. coli OP50 (Stiernagle, 2006)56.
C. elegans KCN survival assay—time-course and dose-response experiments
KCN survival assays were performed as previously described (Del Borrello et al.)18. In brief, 25 μL M9 buffer was distributed to each well of a 96-well flat-bottom culture plate and 0.5 μL chemical solution (or DMSO alone) was added to designated wells (1% DMSO v/v) using either a multi-channel pipette (dose responses) or pinning tool (chemical screens) (V&P Scientific). First larval stage (L1) worms were isolated from mixed-stage plates using 96-well 11 μm nylon mesh filter plates (Millipore Multiscreen) and diluted with M9 to a concentration of ~6 worms/μL; 20 μL worm suspension was added to each well of the microplate. 5 μL freshly prepared 10 X (2 mM) potassium cyanide (KCN) stock solution was then added to each well to achieve 200 μM KCN. Following KCN addition, plates were sealed with aluminum foil and incubated for 15 h at 20 °C with shaking at 165 rpm. After 15 h, KCN in plates was diluted 6-fold with M9 buffer and imaged on a Nikon Ti Eclipse inverted microscope (Nikon NIS Elements AR) every 10 min for 3 h. At each time point, two sequential images (500 ms interval) were taken for each well. Using Python scripts, worm-associated pixels in each image were segregated from background and worm motility (i.e., change in worm-associated pixels) between sequential images was used to estimate worm survival57. The motility score for each well was divided by the motility scores from corresponding DMSO controls, resulting in a relative motility value for each chemical. For chemical screens and dose-response experiments, the relative motility of each well 3 h post dilution of KCN was used as an endpoint. At least three biological replicates were performed for each experiment; relative motility values were averaged across replicates.
Free-living nematode liquid development and viability assay—dose-response experiments
Liquid development and viability assays were adapted from a previously established chemical screening protocol (Burns et al.)24. In brief, a saturated culture of E. coli HB101 was concentrated 2-fold in liquid NGM and 40 μL of this suspension was distributed to each well of a 96-well flat-bottom culture plate. 0.5 μL chemical solution (or DMSO alone) was added to each well (1% DMSO v/v) using either a multi-channel pipette or a pinning tool (V&P Scientific). L1 worms synchronized from an embryo preparation performed the day prior were diluted to a concentration of ~2 worms/μL in M9 buffer; 10 μL worm suspension was then added to each well. Culture plates were sealed with parafilm and placed in a plastic container lined with wet paper towels to prevent evaporation. Containers were then incubated for 6 days at 20 °C with shaking at 165 rpm. Following incubation, 300 μL M9 was added to each well to decrease the turbidity of the HB101-NGM suspension. Single images of each microplate well were then taken on a Nikon Ti Eclipse inverted microscope (Nikon NIS Elements AR). Images were processed using Python scripts to isolate worm-associated pixels from background. Scores of worm development and viability were determined as the sum of worm-associated pixels in each well. Development/viability scores for each well were divided by the development /viability scores for the corresponding DMSO controls, resulting in a relative viability value for each chemical. At least three biological replicates were performed for each dose-response experiment; relative viability values were averaged across replicates.
C. elegans Chemical Screens
NPD libraries were stored in 96-well plates, each containing 80 compounds and 16 DMSO controls (columns 1 and 12). For preliminary screens of NPD libraries, eight 96-well plates containing 617 compounds (400 Pilot library; 80 Authentic library; 137 derivatives) and 128 DMSO controls were screened in triplicate at a concentration of 50 μM for both C. elegans assays described above; data were averaged across the three biological replicates. To account for possible variation among plates, hit identification was performed on a plate-to-plate basis. As drug hits (i.e., outliers) can influence the mean of the distribution of scores, relative motility or relative viability values were converted to robust z-scores using the median and the median absolute deviation (MAD) of each plate. Hits were identified if a compound’s z-score was below −3 (i.e., 3x MAD below the median).
HEK293 cell culture and dose-response experiments
Human embryonic kidney (HEK293; Invitrogen’s Flp-In-293 cell line cat #R75007) cells were grown and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (PenStrep).
To prepare cells for viability testing, 5,000 HEK293 cells/100 μL were seeded into each well of a 96-well flat-bottom tissue culture plate and grown overnight at 37 °C with 5% CO2. 0.5 μL compound from prepared dose-response plates were then added to each well (0.5% v/v DMSO) and cells were allowed to grow for an additional 2 days. After this period of growth, cells were incubated for 4 h with 10 μL CellTiter-Blue viability reagent. Raw scores of cell viability were then determined using a CLARIOstar Plate Reader (560/590 nm) and the fluorometric quantification of the reduction of resazurin to resorufin. “Relative viability” was determined by dividing the background-corrected scores of chemical-treated wells from the corresponding DMSO controls. Data represent the mean of at least three biological replicates.
To prepare cells for mitochondria isolation, cells were grown in 5 ×20 cm dishes containing 20 mL DMEM/10%FBS/1% PenStrep for 3–4 days until confluent at 37 °C with 5% CO2. Following growth, media was removed and plates were rinsed once with 5 mL of PBS. TrypLE Express Enzyme (Thermo Fisher #12604013) was then added dropwise to the plates to completely cover the surface (~2 mL) and plates were incubated at 37 °C for 5 min in order to detach cells. Cell suspension from each plate was then transferred to 18 mL of DMEM/10%FBS/1% Pen Strep media and all samples were pooled (100 mL total volume). Cell number was then determined using a Countess Automated Cell Counter (Thermo Fisher) to ensure an appropriate number of viable cells ( ~ 100,000,000) was obtained. Following counting, cells were pelleted by centrifugation at 200 x g for 5 min at room temperature.
Mitochondria isolation from free-living nematodes
Mitochondria were isolated from the following free-living nematode strains: C. elegans—N2, CB3474, ECA882, ECA917, ECA1075, ECA1080; C. briggsae—AF16; P. pacificus—PS312; and P. hermaphrodita—B178. With the exception of the P. hermaphrodita strain B178, adult worms of each strain were grown in bulk through liquid culture to obtain mitochondria in sufficient quantities. Due to poor growth in liquid media, mixed stage populations of P. hermaphrodita were washed off and collected from NGM plates seeded with OP50. The isolation of mitochondria from all free-living nematode strains followed the protocol previously outlined in Burns et al.24. In brief, a 1 L saturated culture of E. coli HB101 was concentrated 20-fold in 50 mL complete S-medium. ~500,000 L1 worms synchronized from an embryo preparation performed the day prior were added to the S-media/HB101 suspension and grown to adulthood over the course of 3.5 days at 20 °C with shaking at 165 rpm. Following growth, worms were collected in 10 ×15 mL conical tubes and washed 7 times in M9 buffer. Worm suspensions were then combined into a single 15 mL conical tube and diluted to a final volume of 15 mL with M9 buffer. 1 mL aliquots of worm suspension were then distributed to 15 × 1.5 mL screw-cap microcentrifuge tubes and were pelleted at 500 g for 1 min. Excess M9 was aspirated without disturbing the worm pellet. 600 μL ice-cold isolate buffer A (250 mM sucrose, 10 mM Tris (pH 7.5), 1 mM EDTA, 1 mM PMSF (freshly prepared)) and 300 μL cold glass beads were then added to each tube. Tubes were incubated on ice for 10 min, and the worms were then lysed by bead beating (6 x 30 s, with 1 min cooling intervals). Tubes containing worm lysate were centrifuged at 1,000 g for 10 min at 4 °C and the supernatant was transferred to 15 new microcentrifuge tubes on ice. The tubes were centrifuged a second time at 1,000 g for 10 min at 4 °C and the supernatant was once again transferred to 15 new microcentrifuge tubes on ice. These tubes were then centrifuged at 16,000 g for 10 min at 4 °C. The resulting mitochondrial pellet was washed twice by re-suspension in 300 μL of ice-cold isolate buffer B (250 mM sucrose, 10 mM Tris (pH 7.5), 1 mM EDTA). Following the final wash, isolation buffer was aspirated from each tube and the pellets were flash-frozen in ethanol/dry ice and stored at −80 °C until needed.
Mitochondria isolation from mammalian tissue
Six 8–10-week-old C57Bl/6 female mice (Charles River) were freshly dissected, and their livers and hearts collected into separate 1.5 mL microcentrifuge tubes. Organs were flash-frozen in liquid nitrogen and stored at −80 °C prior to use. The heart of a freshly slaughtered adult cow was obtained from a local abattoir (Peel Sausage Inc.), chopped into ~1-inch cubes and collected into 50 mL falcon tubes. Falcon tubes were flash-frozen in a container of dry ice and stored at −80 °C prior to use. Isolation of mitochondria from all mammalian tissue was carried out following the protocol described above. However, due to size, the initial homogenization of tissue was performed by 15 strokes in a dounce homogenizer (mouse heart) or with several 5 s pulses in small Nutribullet blender (cow heart). All mouse protocols for mouse care and dissection were reviewed and approved by the University of Toronto Animal Care Committee, in accordance with the Canadian Council on Animal Care.
Mitochondria isolation from HEK293 cells
Isolation of mitochondria from HEK293 cells was carried out following the same protocol as the isolation of mitochondria from mammalian tissue (described above) with one exception. The initial homogenization of the pelleted cells was performed with 20 up-and-down passes of a Teflon/glass Potter-Elvehjem homogenizer with a 0.1-0.15 mm clearance. Subsequent steps were performed as previously described in Burns et al.24.
Electron transport chain (ETC) assays
The enzymatic activities of individual ETC complexes (I-IV) were assessed spectrophotometrically in isolated mitochondria using a Varioskan LUX Multimode plate reader (ThermoFisher Scientific). Mitochondrial pellets previously isolated were thawed on ice and resuspended in ice-cold isolation buffer (250 mM sucrose, 10 mM Tris (pH 7.5), 1 mM EDTA). The BCA assay (Walker, 1994) was used to quantify the protein concentration of mitochondria suspensions; solutions were diluted to a concentration of 0.2 mg/mL for use in all assays. For the complex I assay, mitochondria pellets were freeze-thawed on ice three times to increase enzyme accessibility. To set up assays, chemical solutions or solvent controls (2.4% solvent v/v) were prepared in 180 μL respective enzyme buffer (see recipes below) and added to each well of a 96-well flat-bottom tissue culture plate. 20 μL mitochondria suspension was then added/mixed with a pipette to initiate the reaction. Immediately following the addition of mitochondria, absorbance was measured (complex I and II: 600 nm; complex III and IV: 550 nm) in 30 s intervals over 15 min. Enzymatic activity was determined in each reaction by plotting absorbance versus time and calculating the slope of the curve during the initial linear phase of the reaction. Any non-specific enzymatic activity was removed by subtracting the slope from corresponding control wells containing enzyme-saturating doses of inhibitor (complex I: 10 μM rotenone; complex II: 100 mM malonate; complex III: 10 μM antimycin A; complex IV: 300 μM KCN). Percent activity of each complex was calculated by dividing the enzyme-specific activity of each well by the enzyme-specific activity of the corresponding solvent controls.
Rotenone-sensitive complex I (NADH: decylubiquinone oxidoreductase) activity was assessed using the 2,6-dichlorophenolindophenol (DCIP)-coupled method previously optimized in Long et al.58. Chemical solutions were prepared in complex I assay buffer composed of 25 mM KPi buffer (pH 7.5), 3 mg/mL bovine serum albumin (BSA), 80 μM NADH, 60 μM decylubiquinone, 160 μM DCIP, 2 μM antimycin A and 2 mM KCN.
Complex II (succinate dehydrogenase) activity was assessed using the DCIP-coupled method previously described in Burns et al.24. Chemical solutions were prepared in complex II assay buffer composed of 1X PBS, 0.35% BSA, 20 mM succinate, 240 μM KCN, 60 μM DCIP, 70 μM decylubiquinone, 25 μM antimycin A, 2 μM rotenone.
Antimycin A-sensitive complex III (decylubiquinol-cytochrome C deductase) activity was determined by measuring the reduction of cytochrome C (CytC) as optimized in Luo et al.59. Decylubiquinol for complex III assays was prepared fresh as described in Janssen and Boyle, 201960. In brief, several flakes of potassium borohydride were mixed into 10 mM decylubiquinone dissolved in ethanol, 0.1 M HCl was then added in 5 μL increments until the solution turned colorless. The solution was spun down at 10,000 g for 1 min to pellet potassium borohydride, and decylubiquinol was transferred to a fresh tube. Chemical solutions were prepared in complex III assay buffer composed of 50 mM Tris-HCl (pH 7.5), 4 mM NaN3, 50 μM decylubiquinol, 50 μM oxidized CytC, 0.01% BSA, and 0.05% Tween-20.
Complex IV (cytochrome C oxidase) activity was determined by following the oxidation of CytC in the protocol outlined by Janssen and Boyle, 201960. Reduced CytC was prepared fresh by adding 1 μL increments of 1 M dithiothreitol to a 1 mM solution of CytC and vortexing. A colour change from brown to orange/pink signals when CytC has been reduced. Reduction of CytC was checked by diluting a sample 50-fold and measuring the ratio of absorbance 550/560 nm (ratio > 6 indicates reduction). Chemical solutions were prepared in complex IV assay buffer composed of 50 mM KPi buffer, 60 μM reduced CytC.
For preliminary screens of hit compounds, compounds were screened at 100 μM; data are the average of at least three biological replicates. The complex I and II activity assays were used for additional dose-response experiments. Assays were performed to cover chemical concentrations ranging from 0.1 nM to 75 μM. For dose responses of benzimidazole analogs, data represent the average of two biological replicates. For all other dose-responses, data represent the average of at least three biological replicates.
Animals
The life cycle of H. polygyrus is maintained at the Swiss Tropical and Public Health Institute (Swiss TPH). The ethical committee of the canton Basel-Stadt authorized all performed animal experiments (permission no. 520). Only trained personnel could execute the experiments.
For in vivo testing, female 3-week old NMRI mice were purchased from Charles River, Germany. The mice were kept in individually ventilated cages (IVC) under environmentally controlled conditions (temperature: 22 °C ± 2 °C; relative humidity: 55% ± 15%; artificial lighting with a circadian cycle of 12 h of light) and had free access to water (municipal tap water supply) and rodent food. Rodents were allowed to acclimatize to the new environment for 1 week before infection. Upon 2 days of arrival, dexamethasone (0.25 mg/L) (Sigma-Aldrich, Switzerland) was added to the normal drinking water for immunosuppression to enable parasite establishment. After 1 week, mice were orally infected with 100 H. polygyrus third larval stage (L3) in 150 µL water.
Aerobic in vitro drug screening in larval and adult H. polygyrus
The eleven benzimidazole derivatives were tested at a concentration of 10 µM under aerobic conditions against L3 and adult H. polygyrus.
For the larval assay, mice feces were collected 2 weeks post-infection and eggs were isolated. The eggs were left on agar at room temperature in the dark for eight to 10 days to allow them to develop into L3. Next, 30–40 L3 were incubated (37 °C, 5% CO2) in the culture medium (RPMI 1640 medium + 1% pen./strep. + 5% amphotericin B) containing the derivatives in 96-well plate (Corning, USA) reaching a final volume of 250 µL for 24 h. For the adult drug screening assay, female NMRI mice were euthanized with CO2 and adult H. polygyrus were dissected out of the intestines. Subsequently, three worms were exposed to the culture medium (RPMI 1640 medium + 1% pen./strep. + 5% amphotericin B) with the derivatives (2 mL) in a 24-well plate (Corning, USA) for either 24 or 72 h. DMSO ( < 1%) served as a negative control in both assays. All conditions were tested in duplicates. Finally, 80 µL of hot water ( ~ 80 °C) for L3 and 500 µL of hot water (~80 °C) for adults were added to the wells to check for their viability under a microscope (Carl Zeiss, Germany). Larvae were categorized as being either alive or dead, depending on whether they moved or not. The adult worms were scored under a microscope based on changes in motility, viability, and morphological alterations at a scale ranging from 0–3 in steps of 0.25 (3 = motile, no changes in morphology or transparency; 2 = reduced motility and/or some damage to tegument noted, as a well as reduced transparency and increased granularity; 1 = severe reduction of motility and/or damage to tegument observed with high opacity and granularity; 0 = dead). The whole experiment was performed two times i.e., two independent biological replicates for both life stages (L3s and adults) and exposure times (24 and 72 h).
Anaerobic in vitro drug screening in adult H. polygyrus
To investigate the impact of the eleven benzimidazole derivatives under anaerobic conditions, an anaerobic chamber with an integrated incubator (Coy Laboratory Products, USA) was used. The same procedure was followed as described for the aerobic drug screening in adult H. polygyrus, but the incubation took place within the anaerobic chamber (37 °C, 85% N2, 10% CO2, 5% H2). Benzimidazole derivatives were tested at a concentration of 10 µM. For scoring, the plates had to be removed from the anaerobic chamber. The whole experiment was performed two times i.e., two independent biological replicates.
In vivo efficacy studies in H. polygyrus mouse model
Four benzimidazole derivatives were tested for in vivo efficacy. The administration of dexamethasone was stopped after 12 days post-infection and 2 days later, the selected four benzimidazole derivatives were administered by oral gavage at a single dose of 200 mg/kg to groups of each four mice. Four mice were left untreated and served as a control group. The derivatives were prepared as a Tween80 (Sigma-Aldrich, Switzerland)/ethanol (Merck, Germany) mixture dissolved in tap water (7:3:90, v/v). After 6–7 days post-dosing, the mice were euthanized with CO2 and dissected. Adult worms were removed from the intestine and counted. The worm burden reduction was calculated by comparing the number of worms found in the control animals versus the treated animals.
Cheminformatics
Chemical structures as supplied by the manufacturers (e.g., RIKEN NPDepo, Vitas-M, ChemBridge Corporation) were analyzed in Python (v. 3.7.4) using the ScoPy library61. Specifically, molecular mass, number of atoms and heteroatoms, number of hydrogen bond donors and acceptors, logP (hydrophobicity), logSw (solubility), and topological polar surface area (TPSA) were computed using the default parameters.
Structural groups of natural product derivatives in the Pilot library were determined by the RIKEN natural product depository. Murcko scaffolds (for compounds with ring systems > 1) were generated for each molecule using DataWarrior (v. 5.5.0), FP2 fingerprints were then generated for each scaffold in Python (v. 3.7.4) using Pybel (v. 3.0.1)62 and pairwise Tanimoto coefficients between fingerprints were used to assess chemical similarity. Scaffolds that shared a Tanimoto coefficient of 0.55 or greater were grouped together. Networks of similar scaffolds within each group of hit compounds in our screen are shown using Cytoscape (v. 3.8.0)48,63.
Statistical analyses
For all dose-response experiments, dose-response curves were fitted using non-linear regression using the DRC package (v. 2.9.4) in R (v. 3.6.1) and the Python rpy2 interface (v. 2.9.4). Inhibitory and lethality concentration 50% values (i.e., IC50 or LC50) parameters were estimated from the fitted curves.
Inclusion and ethics
All authors/collaborators of this study have fulfilled all the requirements required by Nature Portfolio journals and have been included as authors, with each significantly contributing to the design and implementation of the study. This work includes local researchers and includes findings that are locally relevant as determined by local partners. Roles and responsibilities were agreed amongst collaborators ahead of the research. This research has not been severely restricted by the setting, and has not resulted in stigmatization, incrimination, discrimination, or personal risk to participants. Local and regional research was taken into consideration in the citations.
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Code availability
Python code used to process microscope images for C. elegans motility and viability phenotypes, as well as generate time course and dose response plots has been previously published57.
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Acknowledgements
This research was funded by grants 501584 and 5003009 to Andrew Fraser from the Canadian Institute of Health Research. Xènia Serrat is supported by the European Molecular Biology Organization (EMBO ALTF 387-2021). Grants-in-Aid for Transformative Research Area (A) “Latent Chemical Space” (23H04885 to H.O.) from the Ministry of Education, Culture, Sports, Science and Technology, Japan. We thank Prof Peter Roy and Prof Erik Andersen for sharing worm strains; Prof Derek van der Kooy, Brenda Takabe and Daniel Merritt for mouse organs and use of their Dounce homogenizer; and the Peel Sausage company for the beef heart. We also thank Jason Moffat’s group for letting us use their plate reader. Finally, we thank Andrew Burns and Sam Del Borrello for guidance and helpful discussion throughout.
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The primary drug screen was carried out by T.D., the screening of derivatives by T.D. and X.S., the in vitro mitochondrial assays were done by T.D., the cell assays were done by J.S. In the lab of I.S., the natural products were purified and provided by H.H., N.W. and H.O. and the screening in H. polygyrus both in vitro and in vivo by L.I. and J.K. The study was conceived of by A.F. and the paper writing and figure construction by T.D., X.S. and A.F.
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Davie, T., Serrat, X., Imhof, L. et al. Identification of a family of species-selective complex I inhibitors as potential anthelmintics. Nat Commun 15, 3367 (2024). https://doi.org/10.1038/s41467-024-47331-3
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DOI: https://doi.org/10.1038/s41467-024-47331-3
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