Skip to main content

Advertisement

SpringerLink
Log in

Co-transfer of blaNDM-5 and mcr-1 by an IncX3–X4 hybrid plasmid in Escherichia coli

  • Brief Communication
  • Published:

From Nature Microbiology

View current issue Submit your manuscript

Abstract

Carbapenem and colistin are the last-resort antibiotics used for treating multidrug-resistant Gram-negative pathogens. Here, we report, for the first time, co-transfer of resistance to both classes of antibiotics by a mobile IncX3–X4 hybrid plasmid in an Escherichia coli isolate. Spread of such a plasmid is of great concern for clinical therapy, and heightened efforts are needed to control its dissemination.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: Genetic features of pCQ02-121 and proposed models for its formation.

Similar content being viewed by others

References

  1. Liu, Y. Y. et al. Lancet Infect. Dis. 16, 161–168 (2016).

    Article  Google Scholar 

  2. Zurfuh, K. et al. Antimicrob. Agents Chemother. 60, 2594–2595 (2016).

    Article  CAS  Google Scholar 

  3. Perrin-Guyomard, A. et al. Eurosurveillance 21, 30135 (2016).

    Article  Google Scholar 

  4. Du, H. et al. Lancet Infect. Dis. 16, 287–288 (2016).

    Article  CAS  Google Scholar 

  5. Falgenhauer, L. et al. Lancet Infect. Dis. 16, 282–283 (2016).

    Article  CAS  Google Scholar 

  6. Poirel, L. et al. Lancet Infect Dis. 16, 281 (2016).

    Article  CAS  Google Scholar 

  7. Yao, X. et al. Lancet Infect. Dis. 16, 288–289 (2016).

    Article  CAS  Google Scholar 

  8. Sun, J. et al. Antimicrob. Agents Chemother. 60, 5014–5017 (2016).

    Article  CAS  Google Scholar 

  9. McGann, P. et al. Antimicrob. Agents Chemother. 60, 4420–4421 (2016).

    Article  CAS  Google Scholar 

  10. Johnson, T. J. et al. Plasmid 68, 43–50 (2012).

    Article  CAS  Google Scholar 

  11. Espedido, B. A. et al. J. Clin. Pathol. 68, 835–838 (2015).

    Article  CAS  Google Scholar 

  12. Li, A. et al. Antimicrob. Agents Chemother. 60, 4351–4354 (2016).

    Article  CAS  Google Scholar 

  13. Wirth, T. et al. Mol. Microbiol. 60, 1136–1151 (2006).

    Article  CAS  Google Scholar 

  14. Barton, B. M. et al. Anal. Biochem. 226, 235–240 (1995).

    Article  CAS  Google Scholar 

  15. Riley, M. et al. Nucleic Acids Res. 34, 1–9 (2006).

    Article  CAS  Google Scholar 

  16. Harmer, C. J. et al. mBio 6, e01866 (2015).

    Article  CAS  Google Scholar 

  17. Harmer, C. J. et al. mBio 5, e01801 (2014).

    Article  CAS  Google Scholar 

  18. Llosa, M. et al. J. Bacteriol. 176, 3210–3217 (1994).

    Article  CAS  Google Scholar 

  19. Nordmann, P. et al. Emerg. Infect. Dis. 18, 1503–1507 (2012).

    Article  Google Scholar 

  20. Poirel, L. et al. Antimicrob. Agents Chemother. 55, 5403–5407 (2011).

    Article  CAS  Google Scholar 

  21. Lorenzo-Diaz, F. et al. J. Bacteriol. 191, 720–727 (2009).

    Article  CAS  Google Scholar 

  22. Carattoli, A. et al. J. Microbiol. Methods 63, 219–228 (2005).

    Article  CAS  Google Scholar 

  23. Aziz, R. K. et al. BMC Genomics 9, 75 (2008).

    Article  Google Scholar 

  24. Sandegren, L. et al. J. Antimicrob. Chemother. 67, 74–83 (2012).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation and Natural Science Foundation of Guangdong Province, China (U1201214), the Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China (IRT13063), the Natural Science Foundation of Guangdong Province (S2012030006590) and the National Basic Research Program of China (2016YFC1200100).

Author information

Author notes

  1. Jian Sun and Run-Shi Yang: †These authors contributed equally to this work.

Authors and Affiliations

  1. National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, 510642, Guangzhou, China

    Jian Sun, Run-Shi Yang, Liang-Xing Fang, Jing Xia, Liang Li, Xiao-Yue Lv, Jia-Hong Duan, Xiao-Ping Liao & Ya-Hong Liu

  2. College of Veterinary Medicine, South China Agricultural University, 510642, Guangzhou, China

    Jian Sun, Run-Shi Yang, Liang-Xing Fang, Jing Xia, Liang Li, Xiao-Yue Lv, Jia-Hong Duan, Xiao-Ping Liao & Ya-Hong Liu

  3. Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, 50011, Iowa, USA

    Qijing Zhang

  4. Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, 310027, Zhejiang, China

    Youjun Feng

  5. Jiangsu Co-Innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, Jiangsu, China

    Ya-Hong Liu

Authors
  1. Jian Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Run-Shi Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qijing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Youjun Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Liang-Xing Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jing Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Liang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xiao-Yue Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Jia-Hong Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Xiao-Ping Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Ya-Hong Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

J.S., R.-S.Y., Q.Z., Y.F., X.-P.L. and Y.-H.L. designed the study and prepared the manuscript. R.-S.Y., L.-X.F., J.X., L.L., X.-Y.L. and J.-H.D. performed experiments. All authors discussed the results. J.S., R.-S.Y., Q.Z., Y.F., X.L. and Y.-H.L. analysed data. J.S., Q.Z., Y.F. and Y.-H.L. drafted this manuscript.

Corresponding authors

Correspondence to Xiao-Ping Liao or Ya-Hong Liu.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary information

Supplementary Figures 1-3, Supplementary Tables 1-2 (PDF 1331 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sun, J., Yang, RS., Zhang, Q. et al. Co-transfer of blaNDM-5 and mcr-1 by an IncX3–X4 hybrid plasmid in Escherichia coli. Nat Microbiol 1, 16176 (2016). https://doi.org/10.1038/nmicrobiol.2016.176

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1038/nmicrobiol.2016.176

  • Springer Nature Limited

This article is cited by

Search

Navigation