Abstract
“Sasam ” is a crude drug that is defined in the in Korean Herbal Pharmacopoeia as the root of Adenophora triphylla var. japonica (Regel.) Hara or A. stricta Miq., Campanulaceae. The dried roots of the Adenophora spp. are available in markets, and the roots of various species are similar to each other in shape, making it difficult to distinguish one from another using only the outer morphological appearance. Therefore, the present study aimed to establish quality control parameters for pharmacognostic evaluation and differentiation of five Adenophora species and two varieties grown in Korea. Inner morphological evaluation of the root of these plants was accomplished and preliminary chemical analysis was performed by liquid chromatography-mass spectrometry profiling. As a result, significant differences among samples were found in anatomical characteristics such as number and thickness of cork layer, existence of stone cell in cork layer, frequency of vessels, and area of intercellular space. Significant differences were found among the samples in the content of three components including shashenoside I and a new alkyl glycoside, adenophoroside I. These findings could provide the scientific criteria for the proper identification and establishment of standards for the use of “Sasam”.
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WSP and HJK extracted the samples and carried out the anatomical and LC-MS analysis with SJC. AAKK and KMA supervised the anatomical and LC-MS analysis, respectively. WSP, HJK and MJA wrote the manuscript. MCS and MJA created the project and super-vised the laboratory work. MCS and HJC provided critical reading and insightful recommendations of the manuscript. JHP and MJA collected and identified the plant. All of the authors have read the final manuscript and approved the submission.
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Park, W.S., Kim, HJ., Choe, S.J. et al. Anatomical characterization and LC-MS profiling of Adenophora roots from Korea. Rev. Bras. Farmacogn. 29, 695–701 (2019). https://doi.org/10.1016/j.bjp.2019.07.003
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DOI: https://doi.org/10.1016/j.bjp.2019.07.003