Abstract
Solid lipid nanoparticles incorporating Curcuma longa L., Zingiberaceae, curcuminoids were produced by the hot melt emulsion method. A Box-Behnken factorial design was adopted to study the nanoparticles production at different levels of factors such as the percentage of curcuminoids, time of homogeniza-tion and surfactant ratio. The optimized nanoparticles were incorporated into hydrogels for stability, drug release and skin permeation tests. The average nanoparticle sizes were 210.4nm; the zeta poten-tial of -30.40 ±4.16; the polydispersivity was 0.222 ±0.125. The average encapsulation efficiency of curcumin and curcuminoids was 52.92 ± 5.41% and 48.39 ± 6.62%, respectively. Solid lipid nanocapsules were obtained with curcumin load varying from 14.2 to 33.6% and total curcuminoids load as high as 47.7%. The topical formulation containing SLN-Curcuminoids showed good spreadability and stability when subjected to mechanical stress test remained with characteristic color, showed no phase separa-tion and no significant change in pH. As a result of slow release, the nanoparticles were able to avoid permeation or penetration in the pig ear epidermis/dermis during 18h. The topical formulation is sta-ble and can be used in further in vivo studies for the treatment of inflammatory reactions, in special for radiodermitis.
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Authors acknowledge the financial support from CAPES (MSc Scholarship), Fapesp (2011/20872-7) and CNPq (PQ-2).
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CMZ (MSc student) was responsible for most of experimental work (nanocapsules, gel and characterization); RMM developed the nanoencapsulation technique and contributed in all laboratory work and chromatographic analysis. ECC idealized the work and contributed to critical reading of the manuscript. LAPF designed the study, supervised the experiments, critically read and defined the final version of the manuscript. All the authors have read the final manuscript and approved the submission.
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Zamarioli, C.M., Martins, R.M., Carvalho, E.C. et al. Nanoparticles containing curcuminoids (Curcuma longa): development of topical delivery formulation. Rev. Bras. Farmacogn. 25, 53–60 (2015). https://doi.org/10.1016/j.bjp.2014.11.010
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DOI: https://doi.org/10.1016/j.bjp.2014.11.010