Abstract
Physical cues of a biomaterial surface such as topography have received extensive attention due to their ease of engineering and reproducible bioeffects. Although many studies have been reported, the relationship between the topography of biomaterial surface and cell/tissue behaviors could not be well evaluated due to the diversified topographies and experimental environments used in these studies. Here we designed 11 patterns (triangle, rectangle, circle, etc.), and employed them to fabricate two types of topographies (micropit and microcolumn), resulting in 22 surface topographies. We conducted comparative assessments of the bioeffects of these microtopographies on cell and tissue in vitro and in vivo. Our results revealed that grooves formed by continuous micropits exhibited a greater ability to accelerate cell migration. Among them, grooves composed of triangular micropits exhibited the strongest enhancement of cell migration in vitro, reducing fibrous tissue encapsulation and promoting vascular formation in vivo.
摘要
生物材料表面的物理性质, 如拓扑结构, 由于其易于加工和稳定的生物作用而受到广泛关注. 虽然已有许多研究报道了材料表面拓扑结构与细胞的相互作用, 但由于不同研究中使用的微结构和实验环境的多样性, 生物材料表面不同微结构与细胞/组织行为之间的影响关系尚未得到很好的统一评价. 在本研究中, 我们设计了11种图案(三角形、 矩形、 圆形等), 这些图案包含两种类型的形貌(微坑和微柱), 这样 一共得到22种表面拓扑结构. 我们在体外和体内对这些微结构影响细胞和组织的生物作用进行了评估. 我们的研究结果表明, 由连续微坑形成的凹槽微结构促进细胞迁移效果相比其他微结构更强. 其中, 由三角形微坑组成的凹槽在体外对细胞迁移的促进作用最强. 在大鼠皮下模型中, 这种三角形微坑组成的凹槽结构减少了纤维组织的包封, 促进了血管的形成.
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Acknowledgements
This work was supported by Zhejiang Provincial Natural Science Foundation of China (LD22E030008), National Natural Science Foundation of China (U20A20262), the Medical Health Science and Technology Project of Zhejiang Provincial Health Commission (2022483477), and the Fundamental Research Funds for the Central Universities (226-2023-00074). This work was also supported by Zhejiang University K. P. Chao’s High Technology Development Foundation.
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Author contributions Ren KF, Ji J, and Yu L conceived, designed, and supervised the overall project. Wang XW and Zheng HY designed and performed the experiments. All the authors discussed the results and commented on the manuscript.
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Supplementary information Experimental details and supporting data are available in the online version of the paper.
Xing-wang Wang is a PhD candidate at Zhejiang University. His research interests are functional coatings on medical device and interaction between cell and surface topography.
Ke-feng Ren is a Full Professor at Zhejiang University. In 2015, he received the National Science Fund of Zhejiang Province for Distinguished Young Scholars. His research interests are biomaterials, medical coatings, biomimetic material surfaces, and drug/gene delivery.
Jian Ji is the Qiushi Chair Professor and the director of the Institute of Biomedical Macromolecules, the Department of Polymer Science and Engineering, Zhejiang University. His main research interest is the data-driven biomaterials research.
Lu Yu is the Professor of Cardiology in Sir Run Run Shaw Hospital, Zhejiang University. He majors in interventional diagnosis and treatment of arrhythmias, especially the catheter ablation and the left atrial appendage occlusion for patients with atrial fibrillation. His current research focuses on the optimized endothelialization of the occlusion devices.
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Wang, Xw., Zheng, Hy., Wang, J. et al. Impact of micro-scale regular topography on cell and tissue behaviors. Sci. China Mater. 67, 2090–2102 (2024). https://doi.org/10.1007/s40843-024-2917-7
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DOI: https://doi.org/10.1007/s40843-024-2917-7