Abstract
Hypoxia is a hallmark of solid tumors, and it significantly impairs the overall anticancer efficacy, particularly photodynamic therapy (PDT). Herein, a catalase-like nanovesicle with near-infrared light-responsiveness, that is, platinum/gold nanoshell encapsulated chlorin e6 (Ce6)/resveratrol (Res) liposome (Pt@Au-Ce6/Res-Lip), was developed to surmount this intractable issue. The Pt@Au-Ce6/Res-Lip can decompose overexpressed H2O2 in tumor microenvironment to produce vast amounts of O2 for further enhancement of the PDT. Under the 808 nm laser irradiation, the Au nanoshells induced hyperthermia at the lesion site to ablate tumor cells, simultaneously inducing the controlled release of photosensitizer Ce6 and chemotherapeutic agent Res. Moreover, stimulated by 660 nm laser, numerous reactive oxygen species were formed to induce apoptosis and necrosis of tumor cells. With the cascade of trimodal therapeutic modality options (chemotherapy, photothermal therapy, and PDT), the Pt@Au-Ce6/Res-Lip showed ultrahigh tumor inhabitation rate in in vitro and in vivo studies, signifying that the Pt@Au-Ce6/Res-Lip nanovesicle is a promising candidate for effective cancer therapy.
摘要
缺氧作为实体瘤的标志, 严重地影响了整体抗肿瘤治疗效 果, 尤其是光动力疗法. 在本文中, 我们开发了具有近红外光响应 性的类过氧化氢酶纳米囊泡: 铂/金纳米壳包覆的二氢卟酚e6 (Ce6)/白藜芦醇(Res)脂质体(Pt@Au-Ce6/Res-Lips), 以解决这一棘 手的问题. Pt@Au-Ce6/Res-Lips可以分解肿瘤微环境中过表达的过 氧化氢, 从而产生大量氧气, 以增强光动力疗法的效果. 在808 nm 激光照射下, 金纳米壳在病变部位产生高热, 以消融肿瘤细胞, 同时 可控地诱导光敏剂Ce6和化疗药物Res的释放. 此外, 在660 nm激光 的刺激下, 形成了大量的活性氧(ROS)以诱导肿瘤细胞的凋亡和坏 死. 随着三重态治疗模式(化疗、光热治疗和光动力治疗)的级联, Pt@Au-Ce6/Res-Lips在体外和体内研究中均显示出超高的肿瘤抑 制率, 这标志着Pt@Au-Ce6/Res-Lips纳米囊泡将有望成为高效的肿 瘤治疗药物.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Wu W, Shao X, Zhao J, et al. Controllable photodynamic therapy implemented by regulating singlet oxygen efficiency. Adv Sci, 2017, 4: 1700113
Yu W, Liu T, Zhang M, et al. O2 economizer for inhibiting cell respiration to combat the hypoxia obstacle in tumor treatments. ACS Nano, 2019, 13: acsnano.8b07852
Zhang K, Zhang Y, Meng X, et al. Light-triggered theranostic liposomes for tumor diagnosis and combined photodynamic and hypoxia-activated prodrug therapy. Biomaterials, 2018, 185: 301–309
Zhang Y, Wang F, Liu C, et al. Nanozyme decorated metal-organic frameworks for enhanced photodynamic therapy. ACS Nano, 2018, 12: 651–661
Song X, Feng L, Liang C, et al. Liposomes co-loaded with metformin and chlorin e6 modulate tumor hypoxia during enhanced photodynamic therapy. Nano Res, 2016, 10: 1200–1212
Yu Z, Zhou P, Pan W, et al. A biomimetic nanoreactor for synergistic chemiexcited photodynamic therapy and starvation therapy against tumor metastasis. Nat Commun, 2018, 9: 5044
Li X, Kwon N, Guo T, et al. Innovative strategies for hypoxictumor photodynamic therapy. Angew Chem Int Ed, 2018, 57: 11522–11531
Li M, Xia J, Tian R, et al. Near-infrared light-initiated molecular superoxide radical generator: Rejuvenating photodynamic therapy against hypoxic tumors. J Am Chem Soc, 2018, 140: 14851–14859
Song G, Liang C, Yi X, et al. Perfluorocarbon-loaded hollow Bi2Se3 nanoparticles for timely supply of oxygen under near-infrared light to enhance the radiotherapy of cancer. Adv Mater, 2016, 28: 2716–2723
Luo Z, Zheng M, Zhao P, et al. Self-monitoring artificial red cells with sufficient oxygen supply for enhanced photodynamic therapy. Sci Rep, 2016, 6: 23393
Tang W, Zhen Z, Wang M, et al. Red blood cell-facilitated photodynamic therapy for cancer treatment. Adv Funct Mater, 2016, 26: 1757–1768
Fan Y, Zhou T, Cui P, et al. Modulation of intracellular oxygen pressure by dual-drug nanoparticles to enhance photodynamic therapy. Adv Funct Mater, 2019, 29: 1806708
Yang ZL, Tian W, Wang Q, et al. Oxygen-evolving mesoporous organosilica coated prussian blue nanoplatform for highly efficient photodynamic therapy of tumors. Adv Sci, 2018, 5: 1700847
Zhu P, Chen Y, Shi J. Nanoenzyme-augmented cancer sonodynamic therapy by catalytic tumor oxygenation. ACS Nano, 2018, 12: 3780–3795
Ouyang J, Wang L, Chen W, et al. Biomimetic nanothylakoids for efficient imaging-guided photodynamic therapy for cancer. Chem Commun, 2018, 54: 3468–3471
Wang H, Chao Y, Liu J, et al. Photosensitizer-crosslinked in-situ polymerization on catalase for tumor hypoxia modulation & enhanced photodynamic therapy. Biomaterials, 2018, 181: 310–317
Zou MZ, Liu WL, Li CX, et al. A multifunctional biomimetic nanoplatform for relieving hypoxia to enhance chemotherapy and inhibit the pd-1/pd-l1 axis. Small, 2018, 14: 1801120
Li J, Xie C, Huang J, et al. Semiconducting polymer nanoenzymes with photothermic activity for enhanced cancer therapy. Angew Chem Int Ed, 2018, 57: 3995–3998
Hsieh CT, Tzou DY, Jiang MT. Methanol electro-oxidation on Pt nanocatalysts prepared by atomic layer deposition. J Electroanal Chem, 2017, 794: 139–147
Baronia R, Goel J, Tiwari S, et al. Efficient electro-oxidation of methanol using PtCo nanocatalysts supported reduced graphene oxide matrix as anode for DMFC. Int J Hydrogen Energy, 2017, 42: 10238–10247
Gao Z, Xu M, Lu M, et al. Urchin-like (gold core)@(platinum shell) nanohybrids: A highly efficient peroxidase-mimetic system for in situ amplified colorimetric immunoassay. Biosens Bioelectron, 2015, 70: 194–201
Liu Y, Wu H, Li M, et al. pH dependent catalytic activities of platinum nanoparticles with respect to the decomposition of hydrogen peroxide and scavenging of superoxide and singlet oxygen. Nanoscale, 2014, 6: 11904–11910
Luo H, Wang Q, Deng Y, et al. Mutually synergistic nanoparticles for effective thermo-molecularly targeted therapy. Adv Funct Mater, 2017, 27: 1702834
Chen Z, Thiramanas R, Schwendy M, et al. Upconversion nanocarriers encapsulated with photoactivatable ru complexes for near-infrared light-regulated enzyme activity. Small, 2017, 13: 1700997
Zhang D, Qin X, Wu T, et al. Extracellular vesicles based self-grown gold nanopopcorn for combinatorial chemo-photothermal therapy. Biomaterials, 2019, 197: 220–228
Li L, Fu Y, Xu Z, et al. Seedless synthetic branched gold nanoshells for chemo-thermal antitumor therapy. J Mater Chem B, 2020, 8: 5155–5166
Wang M, Liu Y, Zhang X, et al. Gold nanoshell coated thermo-pH dual responsive liposomes for resveratrol delivery and chemophotothermal synergistic cancer therapy. J Mater Chem B, 2017, 5: 2161–2171
Luo L, Bian Y, Liu Y, et al. Combined near infrared photothermal therapy and chemotherapy using gold nanoshells coated liposomes to enhance antitumor effect. Small, 2016, 12: 4103–4112
Li L, Liu H, Bian J, et al. Ag/Pd bimetal nanozyme with enhanced catalytic and photothermal effects for ROS/hyperthermia/chemotherapy triple-modality antitumor therapy. Chem Eng J, 2020, 397: 125438
Luo L, He H, Li C, et al. Near-infrared responsive bimetallic nanovesicles for enhanced synergistic chemophotothermal therapy. ACS Biomater Sci Eng, 2019, 5: 1321–1331
Liu Y, Shu G, Li X, et al. Human HSP70 promoter-based Prussian blue nanotheranostics for thermo-controlled gene therapy and synergistic photothermal ablation. Adv Funct Mater, 2018, 28: 1802026
Li Y, Liu Z, Hou Y, et al. Multifunctional nanoplatform based on black phosphorus quantum dots for bioimaging and photodynamic/photothermal synergistic cancer therapy. ACS Appl Mater Interfaces, 2017, 9: 25098–25106
Shoshan MS, Vonderach T, Hattendorf B, et al. Peptide-coated platinum nanoparticles with selective toxicity against liver cancer cells. Angew Chem Int Ed, 2019, 58: 4901–4905
Wei J, Li J, Sun D, et al. A novel theranostic nanoplatform based on Pd@Pt-PEG-Ce6 for enhanced photodynamic therapy by modulating tumor hypoxia microenvironment. Adv Funct Mater, 2018, 28: 1706310
Wang Y, Huang X, Tang Y, et al. A light-induced nitric oxide controllable release nano-platform based on diketopyrrolopyrrole derivatives for ph-responsive photodynamic/photothermal synergistic cancer therapy. Chem Sci, 2018, 9: 8103–8109
Bian K, Zhang X, Liu K, et al. Peptide-directed hierarchical mineralized silver nanocages for anti-tumor photothermal therapy. ACS Sustain Chem Eng, 2018, 6: 7574–7588
Acknowledgements
This work was financially supported by the National Natural Science Foundation (21776238, 21476190, and 31801198), and Hebei province Natural fund key projects (B2019203479).
Author information
Authors and Affiliations
Contributions
Luo L designed and engineered the samples; Luo L characterized the samples with support from Cong C; Luo L and Li L performed the experiments; He Y performed the data analysis; Hao Z contributed to the theoretical analysis; Luo L wrote the paper with support from Li L; Li L revised the paper and supplemented experiments with the help of Luo L. All authors contributed to the general discussion.
Corresponding author
Additional information
Conflict of interest
The authors declare that they have no conflict of interest.
Liyao Luo received her PhD degree from Yanshan University in 2019. She is currently engaged in related work in the pharmaceutical field.
Lei Li is currently a PhD student in Prof. Dawei Gao’s group at Yanshan University. His main research focuses on the syntheses of noble metal nanomaterials and their applications for antitumor treatment.
Dawei Gao is currently a full professor at Yanshan University. Her research interests include the syntheses of nanomaterials and their bioapplications.
Supplementary Information for
Rights and permissions
About this article
Cite this article
Luo, L., Li, L., Cong, C. et al. Catalase-like nanosystem for interlocking trimodal cancer therapy with hypoxia relief. Sci. China Mater. 64, 1021–1034 (2021). https://doi.org/10.1007/s40843-020-1492-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40843-020-1492-3