Abstract
G-Quadruplexes(GQs), which are formed by G-rich DNA sequences in human telomeres, have become an attractive target for cancer treatment. The ligands to stabilize the conformation of human telomeric GQs in vivo are particularly important for structure-based ligand design and drug development targeting the noncanonical DNA structure. Here we report the conformational conversion of Tel26 induced by a naphthalene diimide(NDI) ligand in K+ buffer, even at cellular physiological temperature(37 °C) and under mimetic cellular crowding conditions created by Ficoll 70. We provide an insight into the dynamic conversion from initial hybrid-2 GQ topology to final parallel GQ topology. These results are helpful for the design of ligands with GQ conformation regulation.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Williamson J. R., Raghuraman M. K., Cech T. R., Cell, 1989, 59(5), 871
Jerry W. S., Woodring E. W., Nat. Rev. Genet., 2019, 20(5), 299
Zhou X., Xing D., Chem. Soc. Rev., 2012, 41(13), 4643
Ruggiero E., Richter S. N., Nucleic Acids Res., 2018, 46(7), 3270
Ahmed A. A., Marchetti C., Ohnmacht S. A., Stephen N., Sci. Rep., 2020, 10(1), 12192
Ahmed A. A., Angell R., Oxenford S., Worthington J., Williams N., Barton N., Fowler T. G., OFlynn D. E., Sunose M., McConvile M., Vo T., Wilson W. D., Karim S. A., Morton J. P., Neidle S., ACS Med. Chem. Lett., 2020, 11(8), 1634
Zhang Z., Dai J., Veliath E., Jones R. A., Yang D., Nucleic Acids Res., 2010, 38(3), 1009
Dai J., Carver M., Punchihewa C., Jones R. A., Yang D., Nucleic Acids Res., 2007, 35(15), 4927
Lin C., Wu G., Wang K., Onel B., Sakai S., Shao Y., Yang D., Angew. Chem. Int. Ed., 2018, 57(34), 10888
Liu W., Zhong Y., Liu L., Shen C., Zeng W., Wang F., Yang D., Mao Z., Nat. Commun., 2018, 9, 3496
Barthwal R., Raje S., Pandav K., Bioorg. Med. Chem., 2020, 28(23), 115761
Yu Q., Wang M., Bioorg. Med. Chem., 2020, 28(17), 115641
Wang Z., Li M., Chen W., Hsu S., Chang T., Nucleic Acids Res., 2016, 44(8), 3958
Xu L., Feng S., Zhou X., Chem Commun., 2011, 47(12), 3517
Xue Y., Kan Z. Y., Wang Q., Yao Y., Liu J., Hao Y. H., Tan Z., J. Am. Chem. Soc., 2007, 129(36), 11185
Heddi B., Phan A. T., J. Am. Chem. Soc., 2011, 133(25), 9824
Xue Y., Liu J., Zheng K., Kan Z., Hao Y., Tan Z., Angew. Chem. Int. Ed., 2011, 50(35), 8046
Hänsel R., Löhr F., Foldynová-Trantirková S., Bamberg E., Trantírek L., Dötsch V., Nucleic Acids Res., 2011, 39(13), 5768
Zuffo M., Guédin A., Leriche E., Doria F., Pirota V., Gabelica V., Mergny J., Freccero M., Nucleic Acids Res., 2018, 46(19), e115
Hao X., Wang C., Wang Y., Li C., Hou J., Zhang F., Kang Q., Gao L., Int. J. Biol. Macromol., 2021, 167, 1048
Takahashi S., Yamamoto J., Kitamura A., Kinjo M., Sugimoto N., Anal. Chem., 2019, 91(4), 2586
Vo T., Oxenford S., Angell R., Marchetti C., Ohnmacht S. A., Wilson W. D., Neidle S., ACS Med. Chem. Lett., 2020, 11(5), 991
Parkinson G. N., Cuenca F., Neidle S., J. Mol. Biol., 2008, 381(5), 1145
Mpima S., A Ohnmacht S. A., Barletta M., Husby J., Pett L. C., Gunaratnam M., Hilton S. T., Neidle S., Bioorg. Med. Chem., 2013, 21(20), 6162
Guo Y., Chen J., Cheng M., Monchaud D., Zhou J., Ju H., Angew. Chem. Int. Ed., 2017, 56(52), 16636
Wu J., Meng Z., Lu Y., Shao F., Chem. Eur. J., 2017, 23(56), 13980
Mashimo T., Yagi H., Sannohe Y., Rajendran A., Sugiyama H., J. Am. Chem. Soc., 2010, 132(42), 14910
Li W., Hou X., Wang P., Xi X., Li M., J. Am. Chem. Soc., 2013, 135(17), 6423
Marchand A., Gabeliaca V., Nucleic Acids Res., 2016, 44(22), 10999
Maleki P., Budhathoki J. B., Roy W. A., Balci H., Mol. Gen. Genomics, 2017, 292(3), 483
Hou X., Fu Y., Wu W., Wang L., Teng F., Xie P., Wang P., Xi X., Nucleic Acids Res., 2017, 45(19), 11401
Acknowledgements
The authors appreciate the technical help of Mr. LI Haoyu(Changchun Institute of Applied Chemistry, Chinese Academy of Sciences) for insightful comments and suggestions.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare no conflicts of interest.
Rights and permissions
About this article
Cite this article
Hao, X., Li, C., Wang, Y. et al. NDI-induced Topological Conversion of Human Telomeric G-Quadruplexes from Hybrid-2 to Parallel Form. Chem. Res. Chin. Univ. 37, 795–800 (2021). https://doi.org/10.1007/s40242-021-1022-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40242-021-1022-9