Abstract
To explore the ability of dihydroxyphenylalanine-insulin-like growth factor-1(DOPA-IGF-1) coated hydroxyapatite/poly(lactic-co-glycolic acid)(HA/PLGA) microspheres to promote the proliferation and osteoclastic differentiation of rabbit bone mesenchymal stem cells(rBMSCs), HA/PLGA microspheres with different HA content (10%, 30%, 50%, mass fraction) were prepared by electrospinning method and HA/PLGA microspheres with 50% HA were coated with IGF-1 and DOPA-IGF-1, respectively. They were co-cultured with rBMSCs, respctively. Cell counting kit-8(CCK-8) detection, confocal laser scanning microscopy(CLSM), alkaline phosphatase(ALP) detection and osteogenesis related genes COL1A1, Runx2 and bone morphogenetic protein-2(BMP-2) detection were conducted to detect the proliferation activity, cell morphology, differentiation ability and the expression level of osteogenesis-related genes of cells cultured on all microspheres groups. The results showed that rBMSCs proliferation increased in an HA content dependent manner, and cells proliferated more in the IGF-1 coated and DOPA-IGF-1 coated groups, in particular in DOPA-IGF-1 coated group, and the differences were more remarkable over time (P<0.05). HA/PLGA microspheres promoted the proliferation and osteogenic differentiation of rBMSCs, and DOPA-IGF-1 coating enhanced the proliferation and osteogenic differentiation of rBMSCs.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Kalsbeek J. H., van Walsum A. D. P., Vroemen J., Janzing H. M. J., Winkelhorst J. T., Bertelink B. P., Roerdink W. H., Bone Joint J., 2018, 100-B(4), 443
Pura J. A., Bobyn J. D., Tanzer M., Clin. Orthop. Relat. Res., 2016, 474(5), 1224
King V., Swart A., Winder M. J., J. Clin. Neurosci., 2016, 32, 91
Nguyen T. H., Lee B. T., Sci. Technol. Adv. Mater., 2012, 13(3), 035002
Nguyen T. H., Lee B. T., J. Mater. Sci.: Mater. M., 2010, 21(6), 1969
Ullah S., Zainol I., Idrus R. H., Int. J. Biol. Macromol., 2017, 104, 1020
Agarwal T., Narayan R., Maji S., Behera S., Kulanthaivel S., Maiti T. K., Banerjee I., Pal K., Giri S., Int. J. Biol. Macromol., 2016, 93, 1499
Shen H., Hu X. X., Yang F., Bei J. Z., Wang S. G., Acta Biomaterialia., 2010, 6(2), 455
Chung H. J., Kim I. K., Kim T. G., Park T. G., Tissue Eng. Pt. A., 2008, 14(5), 607
Cebatariuniene A., Jarmalaviciute A., Tunaitis V., Puriene A., Venalis A., Pivoriunas A., J. Cranio. Maxill. Surg., 2017, 45(6), 845
Lee Y. S., Lim K. S., Oh J. E., Yoon A. R., Joo W. S., Kim H. S., Yun C. O., Kim S., J. Control. Release, 2015, 205, 128
Sivandzade F., Mashayekhan S., J. Biomat. Sci. Polym. E., 2018, 29(6), 683
Kuterbekov M., Machillot P., Lhuissier P., Picart C., Jonas A. M., Glinel K., Acta Biomater., 2018, 75, 300
Yan S., Xia P., Xu S., Zhang K., Li G., Cui L., Yin J., ACS Appl. Mater. Interfaces, 2018, 10(19), 16270
Gao T., Zhang N., Wang Z., Wang Y., Liu Y., Ito Y., Zhang P., Macromol. Biosci., 2015, 15(8), 1070
Wang H., Li P., Zhang L., Zhang J., Ling X., Ran H., China Journal of Chinese Materia Medica, 2009, 34(23), 3021
Singh S. K., Vuddanda P. R., Singh S., Srivastava A. K., Biomed. Research International, 2013, 2013, 909045
Li C., Qian Y., Zhao S., Yin Y., Li J., Mater. Sci. Eng. C, Mater. Biol. Appl., 2016, 64, 43
Malik S. A., Ng W. H., Bowen J., Tang J., Gomez A., Kenyon A. J., Day R. M., J. Colloid Interf. Sci., 2016, 467, 220
Fu C., Yang X., Tan S., Song L., Sci. Rep., 2017, 7(1), 12549
Li Y., Zhang Z., Zhang Z., Cell Tissues Organs, 2018, 205(1), 20
Mao Z., Li Y., Yang Y., Fang Z., Chen X., Wang Y., Kang J., Qu X., Yuan W., Dai K., Yue B., Front Pharmacol., 2018, 9, 368
Poudel S. B., Bhattarai G., Kook S. H., Shin Y. J., Kwon T. H., Lee S. Y., Lee J. C., Growth HormIgf Res., 2017, 36, 1
Rico-Llanos G. A., Becerra J., Visser R., J. Biomed. Mater. Res. A., 2017, 105(7), 1867
Reible B., Schmidmaier G., Moghaddam A., Westhauser F., Int. J. Mol. Sci., 2018, 19(6), 1674
Shu X. L., Feng J., Feng J., Huang X. M., Li L. Q., Shi Q. S., J. Biomater. Appl., 2017, 32(5), 547
Xian L., Wu X., Pang L., Lou M., Rosen C. J., Qiu T., Crane J., Frassica F., Zhang L., Rodriguez J. P., Xiaofeng J., Shoshana Y., Shouhong X., Argiris E., Mei W., Xu C., Nat. Med., 2012, 18(7), 1095
Hawkins-Carranza F. G., Munoz-Calvo M. T., Martos-Moreno G. A., Allo-Miguel G., Del Rio L., Pozo J., Chowen J. A., Perez-Jurado L. A., Argente J., Horm. Res. Paediat., 2018, 89(3), 200
Liu C., Li Y., Wang J., Liu C., Liu W., Jian X., Molecules, 2018, 23(7), 1643
Cho H. J., Perikamana S. K. M., Lee J. H., Lee J., Lee K. M., Shin C. S., Shin H., Acs. Appl. Mater. Inter., 2014, 6(14), 11225
Wang R., Xu B., Xu H. G., Cell Physiol. Biochem., 2017, 41(1), 213
Shi P. J., Wang Q., Yu C. P., Fan F. J., Liu M., Tu M. L., Lu W. H., Du M., Colloids Surf. B Biointerfaces, 2017, 155, 477
Zhou P., Wu J., Xia Y., Yuan Y., Zhang H., Xu S., Lin K., Int. J. Nanomedicine, 2018, 13, 4083
Mostafa N. Z., Uludag H., Varkey M., Dederich D. N., Doschak M. R., El-Bialy T. H., Open Dent. J., 2011, 5, 139
Zhang G., Na Z., Ren B., Zhao X., Liu W., Int. J. Clin. Exp. Med., 2015, 8(8), 12172
Reible B., Schmidmaier G., Moghaddam A. Westhauser F., Int. J. Mol. Sci., 2018, 19(6), 1674
Zhang Z., Li L., Yang W., Cao Y., Shi Y., Li X., Zhang Q., Osteoarthritis Cartilage, 2017, 25(2), 309
Zhang C., Miyatake H., Wang Y., Inaba T., Wang Y., Zhang P. B., Ito Y., Angew. Chem. Int. Edit., 2016, 55(38), 11447
Author information
Authors and Affiliations
Corresponding author
Additional information
Supported by the Special Health Project of Jilin Province, China(No.3D516D703429) and the Special Health Project of Department of Finance of Jilin Province, China (No.3D5177703429).
Rights and permissions
About this article
Cite this article
Liu, G., Li, Y., Yang, S. et al. DOPA-IGF-1 Coated HA/PLGA Microspheres Promoting Proliferation and Osteoclastic Differentiation of Rabbit Bone Mesenchymal Stem Cells. Chem. Res. Chin. Univ. 35, 514–520 (2019). https://doi.org/10.1007/s40242-019-9007-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40242-019-9007-7