Abstract
A rapid and sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method for the determination of cefotetan in human plasma was developed and validated. After the protein precipitation of sample with acetonitrile, the analyte and internal standard(IS), tramadol, were separated on a Zorbax XDB C8 column using acetonitrile/1%(volume fraction) formic acid(volume ratio 35:65, pH=2.5) as mobile phase at a flow rate of 1.0 mL/min with a 1:1 split. The detection was performed by electrospray ionization with positive ion mode, followed by multiple reaction monitoring of the transitions for cefotetan at m/z 576.3→460.2(quantifier) and m/z 576.3→432.2(qualifier) and for IS at m/z 264.1→58.1. Cefotetan and IS were eluted at 1.86 and 1.87 min, respectively. The assay was linear over the concentration range of 0.1–100 μg/mL for 20 μL of human plasma only with intra- and inter-day precisions(expressed as the relative standard deviation) of less than 6.62% and accuracies(as relative error) of ±1.31%. The method was applied to the pharmacokinetic study of a 1-h intravenous infusion of 1.0 g of cefotetan disodium for human volunteers(n=6).
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Supported by the National Natural Science Foundation of China(Nos.81430087, 81473142, 81102383) and the Preject of China Equipment and Education Resources System(CERS)(No.CERS-1-70).
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Shi, M., Yin, L., Cai, L. et al. LC-MS/MS method for the quantitation of cefotetan in human plasma and its application to pharmacokinetic study. Chem. Res. Chin. Univ. 30, 900–904 (2014). https://doi.org/10.1007/s40242-014-4116-9
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DOI: https://doi.org/10.1007/s40242-014-4116-9