Abstract
Tissue specific stem cells were identified in adult stromal tissues. Ultraviolet (UV) radiation induces photo-aging on human skin. Because of UVA irradiation, it is thought that aging phenomenon such as skin elasticity reduction, wrinkle formation, and retardation of wound healing were occurred. In the present study we found that reduction of human dermal stem/progenitor cells (hDSPCs) functionality by UVA irradiation. We enriched hDSPCs from normal human dermal fibroblasts. To develop the screening system for potential anti-aging agent using hDSPCs, we treated UVA in the hDSPCs. To evaluate whether the stemness of hDSPCs is down-regulated in UVA treated hDSPCs, we measured the expression levels of SOX2, NANOG, and S100B, which are well-known representative dermal stem/progenitor cell markers. We observed that UVA-irradiated hDSPCs had lower expression levels of those markers compared with non-treated hDSPCs. Furthermore UVA irradiation reduces the multipotency of hDSPCs to differentiate into adipocytes, chondrocytes, and osteoblasts. In this study, we suggest that chronic aging model by UVA treated hDSPCs can be used for new screening system for anti-aging agents
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References
Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 2002;418:41–49.
Bajada S, Mazakova I, Richardson JB, Ashammakhi N. Updates on stem cells and their applications in regenerative medicine. J Tissue Eng Regen Med 2008;2:169–183.
Talwar HS, Griffiths CE, Fisher GJ, Hamilton TA, Voorhees JJJ. Reduced type I and type III procollagens in photodamaged adult human skin. J Invest Dermatol 1995;105:285–290.
Ghazizadeh S, Taichman LBB. Multiple classes of stem cells in cutaneous epithelium: a lineage analysis of adult mouse skin. EMBO J 2001;20:15–22.
Gola M, Czajkowski R, Bajek A, Dura A, Drewa T. Melanocyte stem cells: biology and current aspects. Med Sci Monit 2012;18:155–159.
Myung P, Andl T, Ito M. Defining the hair follicle stem cell (Part II). J Cutan Pathol 2009;36:1134–1137.
Toma JG, Akhavan M, Fernandes KJ, Barnabé- Heider F, Sadikot A, Kaplan DR, et al. Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 2001;3:778–784.
Toma JG1, McKenzie IA, Bagli D, Miller FDD. Isolation and characteriza-tion of multipotent skin-derived precursors from human skin. Stem Cells 2005;23:727–737.
Shim JH, Park JY, Lee MG, Kang HH, Lee TR, Shin DWW. Human dermal stem/progenitor cell-derived conditioned medium ameliorates ultraviolet a-induced damage of normal human dermal fibroblasts. PLoS One 2013; 11:e67604.
Sorg O, Antille C, Kaya G, Saurat JHH. Retinoids in cosmeceuticals. Dermatol Ther 2006;19:289–296.
Chan ES, Fernandez P, Merchant AA, Montesinos MC, Trzaska S, Desai A, et al. Adenosine A2A receptors in diffuse dermal fibrosis: pathogenic role in human dermal fibroblasts and in a murine model of scleroderma. Arthritis Rheum 2006;54:2632–2642.
Kirkwood TBB. Understanding the odd science of aging. Cell 2005;120: 437–447.
Yaar M, Gilchrest BAA. Photoageing: mechanism, prevention and therapy. Br J Dermatol 2007;157:874–887.
Jones DL, Rando TAA. Emerging models and paradigms for stem cell ageing. Nat Cell Biol 2011;13:506–512.
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Shim, J.H. Screening system establishment for potential anti-aging agent using UVA-induced aging of human dermal stem/progenitor cells. Tissue Eng Regen Med 12 (Suppl 2), 155–161 (2015). https://doi.org/10.1007/s13770-014-0429-5
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DOI: https://doi.org/10.1007/s13770-014-0429-5