Abstract
Plant biotechnology using in-vitro cell and tissue culture is a practical plant breeding tool in developing plants resistant to different abiotic stresses such as cold stress and elevated soil salinity. In this study, the focus is on the in vitro breeding method applied for development of plants resistant to heavy metal (HM) stress. It consists of the following three successive stages: (i) initiation of callus cells, some of which are somaclonal variants with new traits, (ii) exposure of the calli to HMs as selective agents during proliferation for selection of somaclonal variants with enhanced HM-resistance, and (iii) selection of the desirable resistant variants following plant regeneration in the presence of HMs. The whole procedure is more efficient and cost-effective than the conventional breeding methods. Moreover, the plants developed through this approach are not regarded as genetically modified organisms (GMOs), and therefore, did not pose negative public acceptance issues unlike GM plants. However, despite the numerous advantages of this in-vitro breeding approach, it has been employed in a few plant breeding studies to generate HM-resistant plants. The present study outlined the fundamental principles of in vitro breeding and the progress made so far towards development of HM-resistant plants based on this approach.
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Ashrafzadeh, S., Leung, D.M.W. In vitro breeding of heavy metal-resistant plants: A review. Hortic. Environ. Biotechnol. 56, 131–136 (2015). https://doi.org/10.1007/s13580-015-0128-8
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DOI: https://doi.org/10.1007/s13580-015-0128-8