Abstract
Backgrounds: β-Amyloid (Aβ) is a principal constituent of senile plaques in Alzheimer’s disease (AD) and induces neuronal cell death. The molecular mechanism of how Aβ evokes neuronal cell death remains complicated, which were investigated in the present study.
Methods: Using the human neuroblastoma cell line SHSY5Y, we investigated the neurotoxic effects of human β-Amyloid 1–42 (Aβ1–42) aggregates on gene expression profile and protein expression profile by using the Agilent GeneChip Human 1A (V2) Oligo MicroArray, Quantitative Real-time PCR, PF-2D and Western blot analysis.
Results: Our results show that Aβ1–42 specifically influences gene and protein expression such as EGR1, eIF5A, PDE8A, ERp57 and ERp5 in pathways associated with apoptotic process, protein translation, cAMP catabolic process and response to endoplasmic reticulum stress.
Conclusion: Although Genes with significant changes in transcriptomic analysis matched very few of the proteins identified in proteomics analysis, our findings will strengthen our knowledge concerning the molecular mechanisms underlying AD.
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Acknowledgements
This work was supported by National Natural Science Funds of China (No. 81703831), the Budget Project of Shanghai Municipal Education Commission (No. 2014YSN05) and Program of Supporting Young Teachers in Shanghai College, China (No. ZZSZY15003).
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Zhonghao Su declares that he has no conflict of interest. Zhuo Dong declares that he has no conflict of interest. Chunxia Guo declares that she has no conflict of interest. Ying Xu declares that she has no conflict of interest. Shuijin Shao declares that he has no conflict of interest. Zhenxia Qin declares that she has no conflict of interest.
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The article does not contain any studies with human and animal and this study was performed following institutional and national guidelines.
Author contributions
Z.S., Z.D., C.G., Y.X., S.S., and Z.Q. designed the experiments. Z.S. performed the experiments. Z.Q. and Z.S. analyzed the data. Z.S., S.S., and Z.Q. wrote the manuscript. Y.X. gave technical support. All authors reviewed the manuscript.
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Su, Z., Dong, Z., Guo, C. et al. Transcriptomics and proteomics analysis of Aβ (1-42)-induced neurotoxicity. Mol. Cell. Toxicol. 15, 255–264 (2019). https://doi.org/10.1007/s13273-019-0029-5
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DOI: https://doi.org/10.1007/s13273-019-0029-5