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Monitoring the microbial community succession and diversity of Liangzhou fumigated vinegar during solid-state fermentation with next-generation sequencing

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Abstract

This study reveals the microbial community succession and diversity during the whole solid-fermentation processes of naturally fermented Liangzhou fumigated vinegar (LZFV). Dynamics and diversity of microbial community succession in “Daqu” starter and other fermentation stages (starch saccharification, alcoholic fermentation, and acetic acid fermentation) were monitored using a metagenomic approach involving high-throughput sequencing. Meanwhile, dynamic changes of characteristic flavor compounds of vinegar were determined by gas chromatograph (GC) analysis. The result showed that the microbiota composition exhibited rich diversity. Twenty-five bacterial and 18 fungal genera were found in the whole fermentation process where Lactobacillus, Acetobacter, Aspergillus, Saccharomyces, and Alternaria were the predominant microorganisms. Alpha diversity metrics showed that bacterial diversity in Daqu was greater than that in AF and AAF. By contrast, fungal diversity increased from Daqu to AF and decreased in the initial stage (5–8 days) of AAF then remained relatively steady. Hence, these results could help understand dynamics of microbial community succession in continuous fermentation of traditional Chinese vinegars. The LZFV fermentation is a continuous process with spontaneous growth that affects the dynamics of microbial communities. Continuous changes of micro-environment conditions in substrate affect the diversity and structure of microbiota. Microbial growth and metabolism were closely related to the changes in the physicochemical characteristics of the cultures. The microbial flora composition showed rich diversity, and with the increase in brewing time and the change in micro-ecological environmental conditions; the microbial community showed a complex dynamic changes.

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Funding

This study was supported by the Ministry of Science and Technology, P. R. China, under the Nature Science Foundation of China (NSFC) (NO. 31360405), and the Gansu Province Key Laboratory of Food Science and Technology, Gansu Agricultural University, is gratefully acknowledged.

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Authors and Affiliations

  1. College of Food Science and Engineering, Gansu Agricultural University, No.1 Yingmen Village, Anning District, Lanzhou, 730070, Gansu, China

    Jianmin Yun, Fengqin Zhao, Wenwei Zhang, Haijiao Yan, Fengyun Zhao & Duiyuan Ai

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  1. Jianmin Yun
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  2. Fengqin Zhao
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  3. Wenwei Zhang
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  4. Haijiao Yan
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Correspondence to Jianmin Yun.

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Yun, J., Zhao, F., Zhang, W. et al. Monitoring the microbial community succession and diversity of Liangzhou fumigated vinegar during solid-state fermentation with next-generation sequencing. Ann Microbiol 69, 279–289 (2019). https://doi.org/10.1007/s13213-018-1418-z

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