Abstract
Fifty-seven bacteria were isolated from Southern Ocean (Indian sector) water samples which were collected from different latitude and longitude of the ocean. All the isolates were able to grow at 4°C, 20°C, 37°C and tolerable NaCl concentration up to 13.5% (w/v). 29 out of 57 isolates were identified using 16S rDNA amplification and the sequences were submitted to National Center for Biotechnology Information (NCBI). All the isolates were classified by using Ribosomal Database Project (RDP) and found that isolates belongs to Proteobacteria and Bacteriodes. The average G+C content was 56.4%. The isolates were screened for the presence of extracellular enzymes, viz. amylase, catalase, urease, esterase, lipase and protease. The disc diffusion method is used to screen antibiotic production by the isolates against four pathogenic bacteria, viz. Salmonella typhimurium (NCIM 2501), Staphylococcus aureus (NCIM 2122), Bacillus subtilis (NCIM 2193), and Pseudomonas aeruginosa (NCIM 2036). Nine out of 29 were found to be antibiotic producer.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Amann R I, Ludwig W, Schleifer K H. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiology and Molecular Biology Reviews, 59(1): 143–169
Antony R, Krishnan K P, Laluraj C M, et al. 2012. Diversity and physiology of culturable bacteria associated with a coastal Antarctic ice core. Microbiological Research, 167(6): 372–380
Basilio A, González I, Vincente M F, et al. 2003. Patterns of antimicrobial activities from soil actinomycetes isolated under different conditions of pH and salinity. Journal of Applied Microbiology, 93(4): 814–823
Campbell I B, Claridge G G C. 1987. Antarctica: Soils, Weathering Processes and Environment, (Developments in Soil Science 16). Amsterdam, the Netherlands: Elsevier
Chakravorty S, Helb D, Burday M, et al. 2007. A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria. Journal of Microbiological Methods, 69(2): 330–339
Demain A L, Sanchez S. 2009. Microbial drug discovery: 80 Years of progress. The Journal of Antibiotics, 62: 5–16
Giebel H A, Brinkhoff T, Zwisler W, et al. 2009. Distribution of Roseobacter RCA and SAR11 lineages and distinct bacterial communities from the subtropics to the Southern Ocean. Environmental Microbiology, 11(8): 2164–2178
Gupta P, Agrawal HK, Bandopadhyay R. 2015. Distribution pattern of bacteria in the two geographic poles and Southern Ocean from the reported 16S rDNA sequences. Current Science, 108(10): 1926–1930
Gupta P, Sinha D, Bandopadhyay R. 2014. Isolation and screening of marine microalgae Chlorella sp. PR1 for anticancer activity. International Journal of Pharmacy and Pharmaceutical Sciences, 6(10): 517–519
Holding A J, Collee J G. 1971. Routine biochemical tests. Methods in Microbiology, 6A: 2–32
Jannasch H W, Jones G E. 1959. Bacterial populations in sea water as determined by different methods of enumeration. Limnology and Oceanography, 4(2): 128–139
Joseph B, Ramteke P W, Thomas G. 2008. Cold active microbial lipases: Some hot issues and recent developments. Biotechnology Advances, 26(5): 457–470
Kogure K. 1998. Bioenergetics of marine bacteria. Current Opinion in Biotechnology, 9(3): 278–282
Lightfield J, Fram N R, Ely B. 2011. Across bacterial phyla, distantlyrelated genomes with similar genomic GC content have similar patterns of amino acid usage. PLoS One, 6(3): e17677
Lordan S, Ross R P, Stanton C. 2011. Marine bioactives as functional food ingredients: potential to reduce the incidence of chronic diseases. Marine Drugs, 9(6): 1056–1100
Mearns-Spragg A, Bregu M, Boyd K G, et al. 1998. Cross-species induction and enhancement of antimicrobial activity produced by epibiotic bacteria from marine algae and invertebrates, after exposure to terrestrial bacteria. Letters in Applied Microbiology, 27(3): 142–146
Murray A E, Grzymski J J. 2007. Diversity and genomics of Antarctic marine micro-organisms. Philosophical Transactions of the Royal Society B Biological Science, 362(1488): 2259–2271
Nam E S, Ahn J K. 2011. Antarctic marine bacterium Pseudoalteromonas sp. KNOUC808 as a source of cold-adapted lactose hydrolyzing enzyme. Brazilian Journal of Microbiology, 42(3): 927–936
Neufeld J D, Yu Zhongtang, Lam W, et al. 2004. Serial analysis of ribosomal sequence tags (SARST): a high-throughput method for profiling complex microbial communities. Environmental Microbiology, 6(2): 131–144
Oh S, Kogure K, Ohwada K, et al. 1991. Correlation between possession of a respiration-dependent Na+ pump and Na+ requirement for growth of marine bacteria. Applied and Environmental Microbiology, 57(6): 1844–1846
Orsi A H, Whitworth T III, Nowlin W D Jr. 1995. On the meridional extent and fronts of the Antarctic Circumpolar Current. Deep-Sea Research Part I: Oceanographic Research Papers, 42(5): 641–673
Petti C A, Polage C R, Schreckenberger P. 2005. The role of 16S rRNA gene sequencing in identification of microorganisms misidentified by conventional methods. Journal of Clinical Microbiology, 43(12): 6123–6125
Rasmussen R S, Morrissey M T. 2007. Marine biotechnology for production of food ingredients. Advances in Food and Nutrition Research, 52: 237–292
Saadoun I, Hameed K M, Moussauui A. 1999. Characterization and analysis of antibiotic activity of some aquatic actinomycetes. Microbios, 99(394): 173–179
Schmalenberger A, Schwieger F, Tebbe C C. 2001. Effect of primers hybridizing to different evolutionarily conserved regions of the small-subunit rRNA gene in PCR-based microbial community analyses and genetic profiling. Applied and Environmental Microbiology, 67(8): 3557–3563
Shivaji S, Begum Z, Rao S S S N, et al. 2013. Antarctic ice core samples: culturable bacterial diversity. Research in Microbiology, 164(1): 70–82
Struvay C, Feller G. 2012. Optimization to low temperature activity in psychrophilic enzymes. International Journal of Molecular Science, 13(9): 11643–11665
Sokolov S, Rintoul S R. 2009. Circumpolar structure and distribution of the Antarctic Circumpolar Current fronts: 2. Variability and relationship to sea surface height. Journal of Geophysical Research, 114(C11): C11019
Srinivas T N R, Rao S S S N, Reddy P V V, et al. 2009. Bacterial diversity and bioprospecting for cold-active lipases, amylases and proteases, from culturable bacteria of Kongsfjorden and NyÅlesund, Svalbard, Arctic. Current Microbiology, 59(5): 537–547
Steven B, Briggs G, McKay C P, et al. 2007. Characterization of the microbial diversity in a permafrost sample from the Canadian high Arctic using culture-dependent and culture-independent methods. FEMS Microbiology Ecology, 59(2): 513–523
Tamura K, Peterson D, Peterson N, et al. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony method. Molecular Biology and Evolution, 28(10): 2731–2739
Teixeira L C R S, Peixoto R S, Cury J C, et al. 2010. Bacterial diversity in rhizosphere soil from Antarctic vascular plants of Admiralty Bay, maritime Antarctica. The ISME Journal, 4: 989–1001
Vijayakumar A, Duraipandiyan V, Jeyaraj B, et al. 2012. Phytochemical analysis and in vitro antimicrobial activity of Illicium griffithii Hook. f. && Thoms extracts. Asian Pacific Journal of Tropical Disease, 2(3): 190–199
Wang Qiong, Garrity G M, Tiedje J M, et al. 2007. Naïve bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Applied and Environmental Microbiology, 73(16): 5261–5267
Ward P, Whitehouse M, Brandon M, et al. 2003. Mesozooplankton community structure across the Antarctic Circumpolar Current to the north of South Georgia: Southern Ocean. Marine Biology, 143(1): 121–130
Weber T S, Deutsch C. 2010. Ocean nutrient ratios governed by plankton biogeography. Nature, 467: 550–554
Whitworth T III. 1980. Zonation and geostrophic flow of the Antarctic circumpolar current at Drake Passage. Deep-Sea Research Part A. Oceanographic Research Papers, 27(7): 497–507
Wilkins D, Lauro F M, Williams T J, et al. 2013. Biogeographic partitioning of Southern Ocean microorganisms revealed by metagenomics. Environmental Microbiology, 15(5): 1318–1333
Wong C M V L, Tam H K, Alias S A, et al. 2011. Pseudomonas and Pedobacter isolates from King George Island inhibited the growth of foodborne pathogens. Polish Polar Research, 32(1): 3–14
Yoshida A, Nishimura M, Kogure K. 2007. Bacterial community structure in the Sulu Sea and adjacent areas. Deep-Sea Research II: Topical Studies in Oceanography, 54(1–2): 103–113
Zhang Chen, Kim S K. 2010. Research and application of marine microbial enzymes: status and prospects. Marine Drugs, 8(6): 1920–1934
Zhang Laitao, Zhang Wei, Jin Yan, et al. 2008. A comparative study on the phylogenetic diversity of culturable actinobacteria isolated from five marine sponge species. Antonie van Leeuwenhoek, 93(3): 241–248
Author information
Authors and Affiliations
Corresponding author
Additional information
Citation: Gupta Pratibha, Balaji Raju, Parani M, Chandra T S, Shukla P, Kumar Anil, Bandopadhyay Rajib. 2015. Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean (Indian sector) water. Acta Oceanologica Sinica, 34(8): 73–82, doi: 10.1007/s13131-015-0709-5
Rights and permissions
About this article
Cite this article
Gupta, P., Balaji, R., Parani, M. et al. Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean (Indian sector) water. Acta Oceanol. Sin. 34, 73–82 (2015). https://doi.org/10.1007/s13131-015-0709-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13131-015-0709-5