Abstract
Flower formation is one of the main developmental stages in the life cycle of flowering plants that can be used as a model system to elucidate the molecular mechanics that control the developmental process in plants. In the present study, we investigated the floral homeotic and MADS box genes in the polygamadioecious tree Garcinia indica. The differential gene expressions of floral homeotic and MADS box genes in male, female, bisexual flowers, and floral organs such as sepal, petal, stamen, and carpel were studied by employing quantitative real-time, PCR-based assays. Of nine differentially expressed floral genes of the MADS box class, AGL11, the master control gene of ovule identity was found to be expressed 2-fold higher in female carpel whereas the ribosome protein involved in ovule development showed 2×106-fold high expression in the female carpel. The hierarchical clustering grouped these genes into four major clusters: cluster I comprised AGL11, AG, and PMADS2, cluster II comprised AP3 and AGL9, cluster III comprised SEP1 and ELFB, and cluster IV comprised AG pathway and ribosome protein. The clustering found was correlated with the quantitative and qualitative expression of genes.
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Andersen CL, Jensen JL, Orntoft TF. 2004. Normalization of real-time quantitative reverse transcription-pcr data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64: 5245–5250
Angenent GC, Colombo L. 1996. Molecular control of ovule development. Trends Plant Sci. 1: 228–232
Barrett SCH, Yakimowski SB, Field DL, Pickup M. 2010. Ecological genetics on sex ratios in plant populations. Phil. Trans. R. Soc. 365: 2549–2557
Becker Annette, Theissen Gunter. 2003. The major clades of MADS-box genes and their role in the development and evolution of flowering plants. Mol. Phylogenet. Evol. 29: 464–489
Bernier G, Havelange A, Houssa C, Petitjean A, Lejeune P. 1993. Physiological signals that induce flowering. Plant Cell 5: 1147–1155
Bowman JL, Drews GN, Meyerowitz EM. 1991a. Expression of the Arabidopsis floral homeotic geneAGAMOUS is restricted to specific cell types late in flower development. Plant Cell 3: 749–758
Bowman JL, Smyth DR, Meyerowitz EM. 1991b. Genetic interactions among floral homeotic genes of Arabidopsis. Development 112: 1–20
Chen X, Meyerowitz EM. 1999. HUA1 and HUA2 are two members of the floral homeotic AGAMOUS pathway. Mol. Cell 3: 349–360
Cheng Y, Kato N, Wang W, Li J, Chen X. 2003. Two RNA Binding Proteins, HEN4 and HUA1, Act in the Processing of AGAMOUS Pre-mRNA in Arabidopsis thaliana. Dev. Cell 4: 53–66
Colombo L, Franken J, Koetje E, van Went J, Dons HJ, Angenent GC, van Tunen A J. 1995. The petunia MADS box gene FBP11 determines ovule identity. Plant Cell 7: 1859–68
Goto K, Meyerowitz EM. 1994. Function and regulation of the Arabidopsis floral homeotic gene pistallata. Genes Dev. 8: 1548–1560
He Y, Doyle MR, Amasino RM. 2004. PAF1-complex-mediated histone methylation of FLOWERING LOCUS C chromatin is required for the vernalization-responsive, winter-annual habit in Arabidopsis. Genes Dev. 18: 2774–2784
Huang J, Struck F, Matzinger DF, Levings III CS. 1994. Flower-enhanced expression of a nuclear-encoded mitochondrial respiratory protein is associated with changes in mitochondrion number. Plant Cell 6: 439–448
Joseph KS, Murthy HN. 2014. Sexual system of Garcinia indica Choisy: geographic variation in trioecy and sexual dimorphism in floral traits. Plant Syst. Evol. 301: 1065–1071
MacKenzie S, McIntosh L. 1999. Higher plant mitochondria. Plant Cell 11: 571–585
Ng M, Yanofsky M. 2001. Function and evolution of the plant MADS-box gene family. Nat. Rev. Gen. 2: 186–195
Patil RV and Pawar KD. 2019. Comparative de novo flower transcriptome analysis of polygamodioecious tree Garciniaindica. 3 Biotech. 9:72
Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP. 2004. Determination of stablehousekeeping genes, differentially regulated target genes and sample integrity: BestKeeper — excel based tool using pair-wise correlations. Biotechnol. Lett. 26: 509–515
Theissen G, Saedler H. 2001. Floral quartets. Nature 409: 469–471
Theissen G. 2001. Development of floral organ identity: stories from the MADS house. Curr. Opin. Plant Biol. 4: 75–85
Theissen G, Strater T, Fischer A, Saedler H. 1995. Structural characterization, chromosomal localization and phylogenetic evaluation of two pairs of AGAMOUS-like MADS-box genes from maize. Gene 156: 155–166
Thornton B, Basu C. 2011. Real-Time PCR (qPCR) Primer Design Using Free Online Software. Biochemistry and Molecular Biology Education 39: 145–154
van der Krol Alexander R, Brunellel A, Tsuchimoto S, Chua N. 1993. Functional analysis of petunia floral homeotic MADS box gene pMADS1. Genes Dev. 7: 1214–1228
Vandesompele J, De Preter K, Pattyn F, Poppe B, Speleman F. 2002. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3:1–12
Winter KU, Saedler H, Theißen G. 2002. On the origin of class B floral homeotic genes: functional substitution and dominant inhibition in Arabidopsis by expression of an ortholog from the gymnosperm Gnetum. Plant J. 31: 457–475
Xie F, Xiao P, Chen D, Xu L, Zhang B. 2012. miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs. Plant Mol. Biol. 80: 75–84
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This study was funded by DST-SERB (YSS/2015/000319).
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Patil, R.V., Pawar, K.D. Differential Expression Pattern Of MADS Box Genes in Floral Whorls of Garcinia indica. J. Crop Sci. Biotechnol. 22, 363–369 (2019). https://doi.org/10.1007/s12892-019-0171-0
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DOI: https://doi.org/10.1007/s12892-019-0171-0