Abstract
Seed priming improves the seed performance and also helps the seedlings to alleviate the detrimental effects of various stresses. Seed priming is believed to bring about some biochemical changes in the metabolism within the seed, which ultimately favors germination and the further growth stages of the seedlings even under stressed conditions. The present investigation was carried out to study the effect of hydropriming and halopriming in three rice varieties (Neeraja, Vaisakh, and Vytilla 6), with varied abiotic stress tolerance potential under NaCl and PEG stress. In general, the application of both stresses, NaCl and PEG induced retardation of growth and metabolism of the seedlings. However, seed priming treatments could reduce the extent of decrease in these biological attributes. Both hydro- and halopriming resulted in the enhancement of protein, carbohydrate, and photosynthetic pigment content, modulated antioxidant enzyme activities, reduced the lipid peroxidation of biomembranes, and enhanced the photochemistry and mitochondrial activities in rice seedlings subjected to NaCl and PEG stress as compared to non-primed ones. According to the various morphological, physiological, and biochemical characteristics studied in the rice seedlings raised from primed and non-primed seeds, we confirmed that both hydropriming and halopriming had a positive influence on stimulating metabolism in rice seeds, which ultimately resulted in improved seedling vigor and tolerance under NaCl and PEG stress. Halopriming was found to be more efficient than hydropriming in enhancing the seedling vigor, overall growth, and stress tolerance potential of rice varieties.
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Amarjit KN, Kumari S, Sharma DR. 2005. In vitro selection and characterization of water-stress tolerant cultures of bell pepper. Ind. J. Plant Physiol. 10: 14–19
Anwar S, Iqbal M, Raza SH, Iqbal N. 2013. Efficacy of seed preconditioning with salicylic and ascorbic acid in increasing vigor of rice (Oryza sativa L.) seedling. Pak. J. Bot. 45: 157–162
Arnon DI. 1949. Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol. 24: 1–5
Ashraf M, Ali Q. 2008. Relative membrane permeability and activities of some antioxidant enzymes as the key determinants of salt tolerance in canola (Brassica napus L.). Environ. Exp. Bot. 63: 266–273
Ashraf M, Rasul E. 1988. Salt tolerance of mungbean (Vigna radiata) at two growth stages. Plant Soil 110: 63–67
Basra SMA, Farooq M, Tabassum R. 2005. Physiological and biochemical aspects of seed vigour enhancement treatments in fine rice (Oryza sativa L.). Seed Sci. Technol. 33: 623–628
Basra SMA, Farooq M, Afzal I, Hussain M. 2006. Influence of osmopriming on the germination and early seedling growth of coarse and fine rice. Int. J. Agric. Biol. 8: 19–22
Bates LS, Waldren RP, Teare ID. 1973. Rapid determination of free proline for water stress studies. Plant Soil 39: 205–208
Behairy RT, El-Danasoury M, Craker L. 2012. Impact of ascorbic acid on seed germination, seedling growth and enzyme activity of salt stressed fenugreek. J. Medicinally Active Plants 1: 106–113
Benamar A, Tallon C, Macherel D. 2003. Membrane integrity and oxidative properties of mitochondria isolated from imbibing pea seeds after priming or accelerated ageing. Seed Sci. Res. 13: 35–45
Berry SK, Kalra CL, Sehgal RC, Kulkarni SG, Kaur S, Arora SK, Sharma BR. 1988. Quality characteristics of seeds of five okra (Abelmoschus esculentus (L). Moench.) cultivars. J. Food Sci. Technol. 25: 303–305
Birendra P, Shambhoo P. 2011. Standardization of seed hydro-priming time for rice (Oryza sativa L.). J. Hill Agric. 2: 115–118
Chen X, Wang Y, Li J, Jiang A, Cheng Y, Zhang W. 2009. Mitochondrial proteome during salt stress-induced programmed cell death in rice. Plant Physiol. Biochem. 47: 407–415
Conrath U, Beckers GJM, Flors V, Garcia-Agustin P, Jakab G et al. 2006. Priming: getting ready for battle. Mol. Plant Microbe Interact. 19: 1062–1071
Dat J, Vandenabee S, Vranova E, Van Montagu M, Inz´e D, Van Breusegem F. 2000. Dual action of the active oxygen species during plant stress responses. Cell Mol. Life Sci. 57: 779–795
Deivanai S, Xavier R, Vinod V, Timalata K, Lim OF. 2011. Role of exogenous proline in ameliorating salt stress at early stage in two rice cultivars. J. Stress Physiol. Biochem. 7: 157–174
Dey MM, Upadhyay HK. 1996. Yield loss due to drought, cold and submergence in Asia. In RE Evenson, RW Herdt, M Hossain, eds, Rice Research in Asia, Progress and Priorities, Cary NC, Oxford University Press, pp 231–242
Dubey RS. 1997. Photosynthesis in plants under stress-ful conditions. In M Pessarakli, ed, Handbook of Photosynthesis, Marcel Dekker, New York, pp 859–875
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 26: 350–356
Ella ES, Dionisio-Sese ML, Ismail AM. 2011. Seed pre-treatment in rice reduces damage, enhances carbohydrate mobilization and improves emergence and seedling establishment under flooded conditions. AoB Plants plr007, doi:10.1093/aobpla/plr007
El-Samad HMA, Shaddad MAK, Barakat N. 2011. Improvement of plants salt tolerance by exogenous application of amino acids. J. Med. Plants Res. 5: 5692–5699
Farooq M, Basra SMA, Hafeez K. 2005. Seed invigoration by osmohardening in Indica and Japonica rice. Seed Sci. Technol. 34: 181–187
Farooq M, Basra SMA, Rehman HV. 2006a. Seed priming enhances emergence, yield and quality of direct-seeded rice. Int. Rice Res. Notes 31: 42–44
Farooq M, Basra SMA, Rehman HV, Tariq M. 2006b. Germination and early seedling growth as affected by pre sowing ethanol seed treatments in fine rice. Int. J. Agric. Biol. 8: l–20
Gallardo K, Job C, Groot SPC, Puype M, Demol H, Vandekerckhove J, Job D. 2001. Proteomic analysis of Arabidopsis seed germination and priming. Plant Physiol. 126: 835–848
Gaspar T, Penel C, Greppin H. 1975. Peroxidase and isoperoxidase in relation to root and flower formation. Plant Biochem. J. 2: 33–47
Giannopolitis CN, Reis SK. 1977. Superoxide Dismutase I. Occurence in higher plants. Plant Physiol. 59: 309–314
Habib N, Ashraf M, Ahmad MSA. 2010. Enhancement in seed germinability of rice (Oryza sativa L.) by pre-sowing seed treatment with nitric oxide (NO) under salt stress. Pak. J. Bot. 42: 4071–4078
Hageman RH, Reed AJ. 1980. Nitrate reductase from higher plants. In A Sanpietra, ed, Methods in Enzymology, Academic Press, New York, 23, 491–503
Harris D, Joshi A, Khan PA, Gothkar P, Sodhi PS. 1999. Onfarm seed priming in semi-arid agriculture: Development and evaluation in maize, rice and chickpea in India, using participatory methods. Exp. Agric. 35: 15–29
Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ. 2000. Plant cellular and molecular responses to high salinity. Annu. Rev. Plant Physiol. Plant Mol. Biol. 51: 463–499
Heath RL, Packer L. 1968. Photoperoxidation in isolated chloroplasts. I- Kinetics and stochiometry of fatty acid peroxidation. Pak. J. Bot. 125:189–198
Hsu SY, Hsu YT, Kao CH. 2003. The effect of polyethylene glycol on proline accumulation in rice leaves. Biol. Plant. 46: 73–78
Iqbal M, Ashraf M. 2005. Pre-sowing seed treatment with cytokinins and its effect on growth, photosynthetic rate, ionic levels and yield of two wheat cultivars differing in salt tolerance. J. Integr. Plant Biol. 47: 1315–1325
Iqbal M, Ashraf M. 2007. Seed preconditioning modulates growth, ionic relations, and photosynthetic capacity in adult plants of hexaploid wheat under salt stress. J. Plant Nutr. 30: 381–396
Iqbal M, Ashraf M. 2010. Changes in hormonal balance: A possible mechanism of pre-sowing chilling-induced salt tolerance in spring wheat. J. Agron. Crop Sci. 196: 440–454
Iqbal M, Ashraf M. 2013. Gibberellic acid mediated induction of salt tolerance in wheat plants: Growth, ionic partitioning, photosynthesis, yield and hormonal homeostasis. Environ. Exp. Bot. 86: 76–85
Jamil M, Malook I, Parveen S, Naz P, Ali A, Jan SU, Rehman S. 2013. Smoke priming, a potent protective agent against salinity: Effect on proline accumulation, elemental uptake, pigmental attributes and protein banding patterns of rice (Oryza Sativa L.). J. Stress Physiol. Biochem. 9: 169–183
Jisha KC, Vijayakumari K, Puthur JT. 2013. Seed priming for abiotic stress tolerance: an overview. Acta Physiol. Plant. 35: 1381–1396
Kaya MD, Okcu G, Atak M, Cikili Y, Kolsaric O. 2006. Seed treatments to overcome salt and drought stress during germination in sunfower (Helianthus annuus L.), Europ. J. Agron. 24: 291–295
Kameli A, Losel DM. 1993. Carbohydrates and water status in wheat plants under water stress. New Phytol. 125: 609–614
Khush GS. 2005. What it will take to feed 5.0 billion rice consumers in 2030. Plant Mol. Biol. 59: 1–6
Kolloffel C. 1967. Respiration rate and mitochondrial activity in the cotyledons of Pisum sativum during germination. Acta Bot. Neerl. 16: 111–122
Liu J, Liu GS, Qi DM, Li FF, Wang EH. 2002. Effect of PEG on germination and active oxygen metabolism in wild rye (Leymus chinensis) seeds. Acta Prataculture Sin. 11: 59–64
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with Folin-phenol reagent. J. Biol. Chem. 193: 265–275
Mahdavi B, Rahimi A. 2013. Seed priming with chitosan improves the germination and growth performance of ajowan (Carum copticum) under salt stress. Eurasia. J. Biosci. 7: 69–76
Mathew J, Mohanasarida K. 2005. Seed priming on crop establishment and seedling vigour in semi-dry rice (Oryza sativa). Res. Crops 6: 23–25
Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7: 405–410
Mittova V, Guy M, Tal M, Volokita M. 2004. Salinity up-regulates the antioxidative system in root mitochondria and peroxisomes of the wild salt-tolerant tomato species Lycopersicon pennellii. J. Exp. Bot. 55: 1105–13
Mondal S, Viji P, Bose B. 2011. Role of seed hardening in rice variety Swarna (MTU 7029). Res. J. Seed Sci. 4: 157–165
Moosavi A, Afshari RT, Sharif-Zadeh F, Aynehband A. 2009. Effect of seed priming on germination characteristics, polyphenoloxidase, and peroxidase activities of four amaranth cultivars. J. Food Agric Environ. 7: 353–358
Munns R. 2002. Comparative physiology of salt and water stress. Plant Cell Environ. 25: 239–250
Nawaz F, Ashraf MY, Ahmad R, Waraich EA. 2013. Selenium (Se) seed priming induced growth and biochemical changes in wheat under water deficit conditions. Biol. Trace Element Res. 151: 284–293
Oukarroum A, Schansker G, Strasser RJ. 2009. Drought stress effects on photosystem I content and photosystem II thermo tolerance analyzed using Chl a fluorescence kinetics in barley varieties differing in their drought tolerance. Physiol. Plant. 137: 188–199
Patade VY, Sujata B, Suprasanna P. 2009. Halopriming imparts tolerance to salt and PEG induced drought stress in sugarcane. Agric. Ecosyst. Environ. 134: 24–28
Puthur JT. 2000. Photosynthetic events in Sesbania sesban (L.) Merrill in relation to osmotic stress during different developmental stages. Ph.D. Thesis, Jamia Millia Islamia, New Delhi
Randhir R, Shetty K. 2005. Developmental stimulation of total phenolics and related antioxidant activity in light-and dark-germinated corn by natural elicitors. Proc. Biochem. 40: 1721–1732
Rontein G, Basset, Hanson, AD. 2002. Metabolic engineering of osmoprotectant accumulation in plants. Metab. Eng. 4: 49–56
Rouhi HR, Aboutalebian MA, Moosavi SA, Karimi FA, Karimi F, Saman M, Samadi M. 2012. Change in several antioxidant enzymes activity of Berseem clover (Trifolium alexandrinum L.) by priming. Int. J. Agric. Sci. 2: 237–243
Saha P, Chatterjee P, Biswas AK. 2010. NaCl pretreatment alleviates salt stress by enhancement of antioxidant defense system and osmolyte accumulation in mungbean (Vigna radiata L. Wilczek). Ind. J. Exp. Biol. 48: 593–600
Sass RL, Cicerone RJ. 2002. Photosynthate allocations in rice plants: food production or atmospheric methane? J. Plant Physiol. 99: 11993–11995
Scandalios JG. 1993. Oxygen stress and superoxide dismutases. Plant Physiol. 101:7–12
Schmitt N, Dizengremel P. 1989. Effect of osmotic stress on mitochondria isolated from etiolated mung bean and sorghum seedlings. Plant Physiol. Biochem. 27: 17–26
Sing-Tomar R, Mathur S, Allakhverdier SI, Jajoo A. 2012. Changes in PS II heterogeneity in response to osmotic and ionic stress in wheat leaves (Triticum aestivum). J. Bioenerg. Biomembr. 44: 411–419
Sudhir PR, Pogoryelov D, Kovacs L, Garab G, Murthy SDS. 2005. The effects of salt stress on photosynthetic electron transport and thylakoid membrane proteins in the cyanobacterium Spirulina platensis. Biochem. Mol. Biol. 38: 481–485
Tavili A, Zare S, Moosav SA, Enayati A. 2011. Effects of seed priming on germination characteristics of bromus species under salt and drought conditions. Am-Eurasian J. Agric. Environ. Sci. 10: 163–168
Varier A, Vari AK, Dadlani M. 2010. The sub cellular basis of seed priming. Curr. Sci. 99: 450–456
Wang W, Vinocur B, Altman A. 2003. Plant responses to drought, salinity and extreme temperatures: Towards genetic engineering for stress tolerance. Planta 218: 1–14
Wang W, Vinocur B, Shoseyov O, Altman A. 2004. Role of plant heat shock proteins and molecular chaperones in the abiotic stress response. Trends Plant Sci. 9: 244–252
Yagmur M, Kaydan D. 2008. Alleviation of osmotic strength of water and salt in germination and seedling growth of triticale with seed priming treatments. Afr. J. Biotechnol. 7: 2156–2162
Yang CM, Sung JM. 1980. Relations between nitrate reductase activity and growth of rice seedlings. J. Agric. Assoc. China 111: 15–23
Yeh YM, Chiu KY, Chen CL, Sung JM. 2005. Partial vacuum extends the longevity of primed bitter gourd seeds by enhancing their anti-oxidative activities during storage. Sci. Hortic. 104: 101–112
Yuan-Yuan S, Yong-Jian S, Ming-Tian W, Xu-Yi L, Xiang G, Rong H, Jun MA. 2010. Effects of seed priming on germination and seedling growth under water stress in rice. Acta Agron. Sin. 36: 1931–1940
Zhang S, Hu J, Zhang Y, Xie XJ, Knapp A. 2007. Seed priming with brassinolide improves lucerne (Medicago sativa L.) seed germination and seedling growth in relation to physiological changes under salinity stress. Aust. J. Agric. Res. 58: 811–815
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Jisha, K.C., Puthur, J.T. Seed halopriming outdo hydropriming in enhancing seedling vigor and osmotic stress tolerance potential of rice varieties. J. Crop Sci. Biotechnol. 17, 209–219 (2014). https://doi.org/10.1007/s12892-014-0077-2
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DOI: https://doi.org/10.1007/s12892-014-0077-2