Abstract
Thyroid lymphoproliferative disorder is known to be associated with Hashimoto thyroiditis. The major histological types of thyroid lymphoproliferative disorders are malignant lymphoma, such as diffuse large B cell lymphoma, extranodal marginal zone B cell lymphoma of mucosa-associated lymphoid tissue (MALT), and follicular lymphoma. All of these are B cell lymphomas; however, T cell lymphoproliferative disorders represent only a minority of cases. Since T cell lymphoproliferative disorder rarely develops and T cell lymphoma is a heterogeneous entity, it is difficult to concisely summarize this complex topic. Here, we present a case of primary thyroid T cell lymphoproliferative disorder reminiscent of indolent T cell lymphoproliferative disorder of the gastrointestinal tract: the pathological findings from this case also were similar to those of MALT lymphoma such as small lymphoid cells, lymphoepithelial lesions, and a low Ki-67 labeling index. T cell lymphoproliferative disorders with these pathological features have been reported in the thyroid gland, lymph nodes, palate, spleen, and gastrointestinal tract. Furthermore, we reviewed and summarized the studies on thyroid T cell lymphoproliferative disorders and compared the cases and their clinicopathological characteristics.
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Introduction
Primary thyroid T cell lymphoproliferative disorder is extremely rare; therefore, it is difficult to consider it a differential diagnosis. In particular, if the cytology is bland and the morphology is similar to that of extranodal marginal zone B cell lymphoma of mucosa-associated lymphoma tissue (MALT), achieving a precise diagnosis is more difficult. Here, we present a case of peripheral T cell lymphoproliferative disorder of the thyroid, which is composed of small lymphoid cells with a low Ki-67 labeling index. Moreover, we thoroughly reviewed the literature to summarize the clinicopathological features of primary thyroid T cell lymphoproliferative disorder and compared them with those of our case.
Clinical history
A 64-year-old woman presented with a 10-year history of a thyroid mass, the size of which had rapidly increased recently; therefore, she was recommended for surgery. Fine-needle aspiration revealed adenomatous goiter. Laboratory data on admission were as follows: hemoglobin level, 12.5 g/dL (normal range, 11.2–14.6 g/dL); white blood cell count, 9.97 × 109/L (normal range, 3.15–8.63 × 109/L); platelet count 258 × 109/L (normal range, 138–347 × 109/L); lactate dehydrogenase level, 198 IU/L (normal range, < 250 IU /L); β2-microglobulin level, 2.00 mg/L (normal range, 0.81–2.19 mg/L); thyroid-stimulating hormone level, 7.61 μIU/mL (normal range, 0.27–4.2 μIU/mL); T3 level, 127.0 ng/dL (normal range, 80–200 ng/dL); free T4 level, 0.58 ng/dL (normal range, 0.93–1.70 ng/dL); anti-thyroglobulin level, > 10,000.0 IU/mL (normal range, 0–4.1 IU/mL); and anti-thyroid peroxidase antibody level, 26.3 IU/mL (normal range, 15–65 IU/mL). On physical examination, a solid mass was palpable on the neck. Computed tomography (CT) of the thyroid revealed a diffusely enlarged thyroid gland (Fig. 1a). Lymph node enlargement was also found in bilateral lateral neck (Fig. 1b). Total thyroidectomy with central lymph node dissection was performed. The diagnosis was peripheral T cell lymphoproliferative disorder, associated with Hashimoto thyroiditis. Post-operative neck CT revealed the decrease in the size of small lymph nodes. Chest, abdominal, and pelvic CT exhibited no remarkable abnormality. As revealed through positron emission tomography, lymph nodes in the right neck level III and left neck levels II–IV showed mild uptake of 18F-fluorodeoxyglucose, indicating that the figures were indeterminate for malignancy. Core needle biopsy was performed on the ovoid-shaped, slightly enlarged lymph nodes in the neck at level II on the left side, in which benign lymphadenopathy was radiologically suspected. Hematoxylin and eosin staining showed no evidence of T cell lymphoproliferative disorder. While the physicians were assessing cyclophosphamide, hydroxydaunorubicin, vincristine, etoposide, and prednisone chemotherapy, she refused bone marrow evaluation and discharged. She had been well at follow-up after discharge.
Materials and methods
Immunohistochemistry and Epstein Barr virus-encoded ribonucleic acid (EBER) in situ hybridization (ISH)
Immunohistochemical staining was performed using the Vantaa BenchMark ULTRA automatic immunostainer and Leica BOND MAX automated system according to the manufacturers’ instructions. The following primary antibodies were used: anti-bcl-2 (Bcl-2/100/D5), anti-bcl-6 (NCL-L-Bcl-6–564), anti-CD3 (LN10), anti-CD4 (SP35), anti-CD8 (SP57), anti-CD10 (56C6), anti-CD20 (L26), anti-CD30 (Ber-h2), anti-CD56 (1B6), anti-CXCL-13 (AF801), anti-Ki-67 (MIB-1), anti-pan-cytokeratin (AE1/AE3), anti-PD-L1 (SP263), anti-terminal deoxynucleotidyl transferase (TdT) (SEN28), anti-T cell receptor (TCR) beta F1 (8A3), anti-TCR gamma (H-41), and anti-T cell intracellular antigen-1 (TIA-1) (2G9A10F5). EBER ISH was performed using the Ventana Benchmark ULTRA automatic immunostainer according to the manufacturer’s instructions (EBER1 DNP Probe).
Immunoglobulin heavy chain (IgH) gene and T cell receptor (TCR) gene rearrangement study
DNA was extracted from formalin-fixed paraffin-embedded tissue, and IgH gene and TCRγ gene rearrangement polymerase chain reactions (PCRs) were performed using IdentiClone™ IGH Gene Clonality Assay and IdentiClone™ TCRG Gene Clonality Assay. To cover IgH gene, primers that targeted conserved framework (FR1-3) and diversity (DH1-7) regions and the downstream consensus JH gene segments were used. The probes for the gene for TCRγ targeted Vγlf, Vγ9, Vγ10, Vγ11, Jγ1.3/2.3, and Jγ1.1/2.1.
Results
Macroscopic and microscopic features
The thyroid glands weighed 121 g. The lobes were well-encapsulated and diffusely enlarged. The cut surface was homogeneous and yellow without evidence of hemorrhage or necrosis (Fig. 1c). Small, mildly cleaved lymphoid cells with marked proliferation infiltrated the thyroid follicles (Fig. 2a and b), thus forming lymphoepithelial lesions similar to those in MALT lymphoma (Fig. 3c). Reactive lymphoid follicles were distributed throughout the thyroid (Fig. 2d). Plasma cells and eosinophils were rarely observed. Prominent arborizing high endothelial venules were not observed. The thyroid follicular cells showed oncocytic change that are occasionally found in Hashimoto thyroiditis. Four small (< 1 cm in size) perithyroidal lymph nodes excised from the central part of the neck showed effacement of nodal architecture. The sinuses were filled with small lymphoid cells, which were morphologically identical to tumor cells of the thyroid (Fig. 2e and f).
Immunohistochemical staining and EBER ISH
The tumor cells were positive for CD3 (Fig. 2g) and CD4 (Fig. 2h), suggesting that the tumor originated from a helper T cell. Sparsely scattered small lymphoid cells were positive for CD8 (Fig. 2i). The CD4:CD8 ratio was 9:1. CD20 did not react with tumor cells and was only expressed in lymphoid follicles. Cytokeratin staining highlighted the lymphoepithelial lesions. The Ki-67 labeling index was 10% in the interfollicular area. EBER ISH showed negative results. CD10, bcl-2, bcl-6, CD30, CD56, CXCL-13, TdT, and TIA-1 were all negative. Tumor cells were positive for TCR beta F1 and CD5, and negative for TCRγ.
IgH and TCRγ gene rearrangement
PCR analysis of the TCRβ (Fig. 3a–d) and TCRγ (Fig. 3e and f) genes revealed clonal gene rearrangement (dominant predominant peaks) in the thyroid gland (Fig. 3a, c, and e) and lymph node samples (Fig. 3b, d, and f). Each pair had similar clonal TCRβ and TCRγ rearrangement patterns. In contrast, IgH had a polyclonal background.
Discussion
Considering the histologic features, the main pathologic differential diagnosis in this case was Hashimoto thyroiditis although TCR gene rearrangement PCR showed monoclonality. Degeneration of follicular cells in Hashimoto thyroiditis occurs via CD8 + T cell-mediated cell death, cytokine-mediated cell death through the activation of CD4 + Th1 cells, and antibody-dependent cell-mediated cytotoxicity. Therefore, dense infiltration of CD4 + and CD8 + lymphocytes is expected in Hashimoto thyroiditis. In the present case, lymphoepithelial lesions and interfollicular areas were diffusely positive for CD4. However, CD8-positive cells were rarely found throughout the thyroid gland. Moreover, TCR monoclonality is detected in both the thyroid gland and lymph node, which shows almost identical clonal rearrangement patterns. Considering these features, the diagnosis in the present case was T cell lymphoproliferative disorder. The World Health Organization (WHO)’s 2017 edition of hematopoietic and lymphoid tissues added this entity: indolent T cell lymphoproliferative disorder of the gastrointestinal tract [1]. This entity consists of monotonous mature-appearing small round lymphoid cells with an indolent clinical course [1]. Table 1 presents a summary of the primary thyroid T cell lymphoproliferative disorders in the literature. There were five cases with pathological findings similar to those in our case [2,3,4]. Interestingly, Hayashi et al. suggested a new subgroup of T cell lymphoma, “indolent peripheral T cell lymphoma,” characterized by small monotonous lymphoid cells with mild nuclear atypia, low Ki-67 labeling index, and good prognosis compared to conventional peripheral T cell lymphoma, NOS [3]. If we consider that the reported cases often have missed Ki-67 labeling indices or mitosis value, there are several reported cases similar to ours [5,6,7,8,9,10]. In summary, thyroid T cell lymphoproliferative disorder mimicking indolent T cell lymphoproliferative disorder of the gastrointestinal tract occurs in the age range of 32–81. They were found at an almost equal frequency in both males and females. Eighteen of 19 patients were alive. We were not able to confirm if the patient who died could be classified as an indolent T cell lymphoproliferative disorder because the proliferative index or mitotic count has not been mentioned. Considering these points, it is reasonable that this disease can be classified as a T cell lymphoproliferative disorder at extranodal sites. However, these pathological features do not always indicate good prognosis. Of the patients with nodal “small-cell peripheral T-cell lymphoma” with a low Ki-67 labeling index, all three patients died excluding two who could not be followed up [11]. Despite the “indolent” pathological findings, most of these patients showed aggressive clinical behavior such as systemic lymph node enlargement or bone marrow involvement [11]. Additionally, this case had lymphoepithelial lesions, similar to the pathological features of MALT lymphoma. Uherova et al. analyzed eight patients in whom peripheral T cell lymphomas resembling MALT lymphoma: 4 patients with T cell lymphomas of the lymph nodes, 1 with T cell lymphoma of the lymph node and salivary gland, 1 with T cell lymphoma of the lymph node and tonsil, 1 with T cell lymphoma of the hard palate, and 1 with T cell lymphoma of the tongue [12].
The relationship between autoimmune thyroiditis and MALT lymphoma is well known. However, the number of cases with primary thyroid T cell lymphoproliferative disorder including T cell lymphoma is extremely low, making it difficult to determine whether primary T cell lymphoproliferative disorder is correlated with autoimmune thyroiditis. Based on our review, 9 of 19 cases (47.4%) with T cell lymphoproliferative disorder, including our case, had Hashimoto thyroiditis or chronic thyroiditis. In autoimmune diseases, almost all infiltrative immune cells, which are CD4 + T cells, react to T1 chemokine receptors such as CXCR3 + and CCR5 + , which are expressed in the thyroid glands [5, 19]. Koida et al. presumed that chronic stimulation of CD4 + T cells leads to the development of a neoplastic clone [5]. This theory cannot definitively explain the development of primary T cell lymphoproliferative disorders of CD8 + T cells.
It is unclear whether fine-needle aspiration cytology is useful in the diagnosis of thyroid lymphoproliferative disorders including malignant lymphoma. Although additional examinations such as flow cytometry and immunohistochemistry can help in the diagnosis of thyroid lymphoproliferative disorder [20], they are performed only when clinicians suspect malignant lymphoma as a differential diagnosis. Sangalli et al. reported that cytological studies of the thyroid showed 100% accuracy (8/8) in the diagnosis of high-grade diffuse large B cell lymphoma [21]. However, ten cases of low-grade MALT lymphoma were diagnosed with Hashimoto thyroiditis. As shown in Table 1, only three out of five cases of T cell lymphoproliferative disorder were diagnosed as malignant lymphoma or atypical lymphoid cells based on fine-needle aspiration. In only one case diagnosed as malignant lymphoma, fine-needle aspiration was performed with immunohistochemistry of CD3 and CD20 as well as TCRγ gene rearrangement PCR analysis [9]. The cytological diagnosis in the present case was an adenomatous goiter. Therefore, flow cytometry or immunohistochemistry is recommended for conventional cytology if malignant lymphoma is clinically suspected.
Data availability
Data and images used in this study were provided by the Inje Biobank of Inje University Busan and Haeundae Paik Hospital, a member of Korea Biobank Network (IJB-20–09).
Code availability
Not applicable.
References
Swerdlow SH, Campo E, Harris NL, Jaffe ES, Pileri SA, Stein H, Thiele J (2017) WHO classification of tumours of haematopoietic and lymphoid tissues, vol 2. WHO classification of tumours, revised 4th edn. World Health Organization, Lyon. https://publications.iarc.fr/Book-And-Report-Series/Who-Classification-Of-Tumours/WHO-Classification-Of-Tumours-Of-Haematopoietic-And-Lymphoid-Tissues-2017
Reeders J, Thant A, de Malmanche T, Pasupathy A (2017) Primary thyroid T-cell lymphoma arising in a middle-aged Caucasian male. Pathology 49(6):647–649. https://doi.org/10.1016/j.pathol.2017.04.012
Hayashi E, Takata K, Sato Y, Tashiro Y, Tachiyama Y, Sawada-Kitamura S, Hiramatsu Y, Sugiguchi S, Nose S, Hirokawa M, Ando M, AbdAlkader L, Maeda Y, Tanimoto M, Yoshino T (2013) Distinct morphologic, phenotypic, and clinical-course characteristics of indolent peripheral T-cell lymphoma. Hum Pathol 44(9):1927–1936. https://doi.org/10.1016/j.humpath.2013.03.002
Kim NR, Ko YH, Lee YD (2010) Primary T-cell lymphoma of the thyroid associated with Hashimoto’s thyroiditis, histologically mimicking MALT-lymphoma. J Korean Med Sci 25(3):481–484. https://doi.org/10.3346/jkms.2010.25.3.481
Koida S, Tsukasaki K, Tsuchiya T, Harasawa H, Fukushima T, Yamada Y, Ohshima K, Kamihira S, Kikuchi M, Tomonaga M (2007) Primary T-cell lymphoma of the thyroid gland with chemokine receptors of Th1 phenotype complicating autoimmune thyroiditis. Haematologica 92(3):e37-40. https://doi.org/10.3324/haematol.10351
Mishra P, Banerjee D, Gujral S (2016) T-Cell lymphoma of thyroid gland with Lennert type of morphology: a case report and review of the literature. Head Neck Pathol 10(3):321–326. https://doi.org/10.1007/s12105-016-0711-z
Motoi N, Ozawa Y (2005) Malignant T-cell lymphoma of the thyroid gland associated with Hashimoto’s thyroiditis. Pathol Int 55(7):425–430. https://doi.org/10.1111/j.1440-1827.2005.01848.x
Raftopoulos I, Vanuno D, Kouraklis G (2001) Two unusual sites of colon cancer metastases and a rare thyroid lymphoma. Case 3. Primary T-cell lymphoma of the thyroid arising in a background of Hashimoto’s thyroiditis. J Clin Oncol 19(15):3576–3580. https://doi.org/10.1200/JCO.2001.19.15.3576
Yang H, Li J, Shen T (2008) Primary T-cell lymphoma of the thyroid: case report and review of the literature. Med Oncol 25(4):462–466. https://doi.org/10.1007/s12032-008-9059-x
Yoshida N, Nishikori M, Izumi T, Imaizumi Y, Sawayama Y, Niino D, Tashima M, Hoshi S, Ohshima K, Shimoyama M, Seto M, Tsukasaki K (2013) Primary peripheral T-cell lymphoma, not otherwise specified of the thyroid with autoimmune thyroiditis. Br J Haematol 161(2):214–223. https://doi.org/10.1111/bjh.12255
Li YL, Liu WP, Tang Y, Zhao S, Zuo Z, Yang YH, Yang QP, Luo TY (2009) Small cell variant of peripheral T-cell lymphoma, not otherwise specified: a clinicopathologic and immunophenotypic analysis. Zhonghua Bing Li XueZaZhi 38(5):323–328. https://doi.org/10.3760/cma.j.issn.0529-5807.2009.05.008
Uherova P, Ross CW, Finn WG, Singleton TP, Kansal R, Schnitzer B (2002) Peripheral T-cell lymphoma mimicking marginal zone B-cell lymphoma. Mod Pathol 15(4):420–425. https://doi.org/10.1038/modpathol.3880541
Colovic M, Matic S, Kryeziu E, Tomin D, Colovic N, Atkinson HD (2007) Outcomes of primary thyroid non-Hodgkin’s lymphoma: a series of nine consecutive cases. Med Oncol 24(2):203–208. https://doi.org/10.1007/BF02698041
Mizukami Y, Michigishi T, Nonomura A, Nakamura S, Hashimoto T, Katsuda S, Otake S, Matsubara F (1990) Primary lymphoma of the thyroid: a clinical, histological and immunohistochemical study of 20 cases. Histopathology 17(3):201–209. https://doi.org/10.1111/j.1365-2559.1990.tb00708.x
Okamoto A, Namura K, Uchiyama H, Kajita Y, Inaba T, Nakamura S, Shimazaki C (2005) Cytotoxic T-cell non-Hodgkin’s lymphoma of the thyroid gland. Am J Hematol 80(1):77–78. https://doi.org/10.1002/ajh.20393
Suzuki Y, Sakane S, Sasaki M, Ueda S, Makino S, Matsuzuka F, Takamatsu J, Ohsawa N (1994) A case of primary thyroid T-cell lymphoma with infiltration of lymphoma cells in peripheral blood: diagnosed by DNA analysis. Nihon Naibunpi Gakkai Zasshi 70(10):1055–1062. https://doi.org/10.1507/endocrine1927.70.10_1055
Yang L, Wang A, Zhang Y, Mu Y (2015) 12 cases of primary thyroid lymphoma in China. J Endocrinol Invest 38(7):739–744. https://doi.org/10.1007/s40618-015-0250-6
Yokoyama J, Ito S, Ohba S, Fujimaki M, Sato E, Komatsu N, Ikeda K, Hanaguri M (2012) Problems of primary T-cell lymphoma of the thyroid gland–a case report. World J Surg Oncol 10:58. https://doi.org/10.1186/1477-7819-10-58
GarcÍa-López MA, Sancho D, Sánchez-Madrid F, Marazuela M (2001) Thyrocytes from autoimmune thyroid disorders produce the chemokines IP-10 and Mig and attract CXCR3+ lymphocytes. J Clin Endocrinol Metab 86(10):5008–5016. https://doi.org/10.1210/jcem.86.10.7953
Cha C, Chen H, Westra WH, Udelsman R (2002) Primary thyroid lymphoma: can the diagnosis be made solely by fine-needle aspiration? Ann Surg Oncol 9(3):298–302. https://doi.org/10.1007/BF02573069
Sangalli G, Serio G, Zampatti C, Lomuscio G, Colombo L (2001) Fine needle aspiration cytology of primary lymphoma of the thyroid: a report of 17 cases. Cytopathology 12(4):257–263. https://doi.org/10.1046/j.1365-2303.2001.00338.x
Acknowledgements
The authors sincerely thank Dr. J. E. Kim at Seoul National University Boramae Hospital and Dr. S. Y. Park at National Cancer Center in South Korea for providing additional immunohistochemical staining or molecular studies.
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BK designed and edited the study protocol. KHN reviewed clinical and pathological data. BK made pathologic diagnosis and took microscopic pictures. BK and KHN reviewed and contributed to the writing of the manuscript.
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Nam, K.H., Kim, B. Primary thyroid T cell lymphoproliferative disorder with a 10-year history of neck mass: a case report and literature review. J Hematopathol 14, 243–250 (2021). https://doi.org/10.1007/s12308-021-00448-6
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DOI: https://doi.org/10.1007/s12308-021-00448-6