Abstract
Gram-staining-negative, uniflagellated, rod-shaped, designated as DCY110T, was isolated from sludge located in Gangwon province, Republic of Korea. The phylogenetic tree of 16S rRNA gene sequence showed that the strain DCY110T belonged to the genus Rhodoferax with a close similarity to Rhodoferax saidenbachensis DSM 22694T (97.7%), Rhodoferax antarcticus DSM 24876T (97.5%), Rhodoferax ferrireducens DSM 15236T (97.3%), and Rhodoferax fermentans JCM 7819T (96.7%). The predominant isoprenoid quinine was ubiquinone (Q-8). DNA G + C content was 62.8 mol%. The major polar lipids were phosphatidylethanolamine and two unidentified phospholipids. The major fatty acids (> 10%) were C12:0, C16:0, summed feature 3 (which comprised C16:1 ω7c and/or C16:1 ω6c). The DNA-DNA relatedness values between the strain DCY110T and the closely related relatives used in this study were lower than 70%. Based on the following polyphasic analysis, the strain DCY110T is considered as a novel species of the genus Rhodoferax, for which the name Rhodoferax koreense sp. nov. is proposed. The type strain is DCY-110T (= KCTC 52288T = JCM 31441T).
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The NCBI GenBank accession number for the 16S rRNA gene sequence of strain DCY110T is KU519435.
Supplemental material for this article may be found at http://www.springerlink.com/content/120956.
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Supplementary data Fig. S1. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, showing the taxonomic position of strain DCY110T in the genus Rhodoferax
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Farh, M.EA., Kim, YJ., Singh, P. et al. Rhodoferax koreense sp. nov, an obligately aerobic bacterium within the family Comamonadaceae, and emended description of the genus Rhodoferax . J Microbiol. 55, 767–774 (2017). https://doi.org/10.1007/s12275-017-7033-z
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DOI: https://doi.org/10.1007/s12275-017-7033-z