Abstract
Clonal cell lines stably expressing a target protein are a common tool in research, but the generation of such cell lines is laborious and time-consuming. In contrast, the novel ExoIN technology guarantees easy-to-handle and fast cell line generation. By expressing selection marker and target as a polyprotein that is efficiently cleaved at the ribosome, unmodified free protein is homogeneously expressed in all cells of the stable cell population.
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McFarland DC (2000) Preparation of pure cell cultures by cloning. Methods Cell Sci 22:63–66
Varshavsky A (2005) Ubiquitin fusion technique and related methods. Methods Enzymol 399:777–799
Matentzoglu K, Scheffner M (2009) Ubiquitin-fusion protein system: a powerful tool for ectopic protein expression in mammalian cells. BioTechniques 46:21–28
Tan CP, Feng Y, Zhou Y-P et al. (2008) Selective small-molecule agonists of G protein-coupled receptor 40 promote glucose-dependent insulin secretion and reduce blood glucose in mice. Diabetes 57:2211–2219
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Baumann, B., Lenz, D. ExoIN-Technologie — ein schneller Weg zu stabilen Assay-Zelllinien. Biospektrum 20, 648–649 (2014). https://doi.org/10.1007/s12268-014-0500-8
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DOI: https://doi.org/10.1007/s12268-014-0500-8