Abstract
CD147 belongs to immunoglobulin superfamily and can stimulate the surrounding fibroblasts to secret matrix metalloproteinases (MMPs). Studies showed that when compared with their normal counterparts, CD47 expression level increased in lung carcinoma tissue, breast cancer tissue, and bladder cancer tissue. They increase in line with a tumor’s malignant progression, invasiveness, and metastasis. However, the precise implications and utility of the presence of CD147 in the WHO grading system for gliomas have rarely been reported; in addition, the signal transduction pathways regarding CD147 remain unclear and controversial. Thus, in performing a meta-analysis, it is essential to reach a reliable conclusion. The related literatures were incorporated into the present meta-analysis after careful assessment, and odds ratios (ORs) with 95 % confidence intervals (95 % CIs) were calculated. Heterogeneity evaluation was estimated. Ten studies involving 615 patients were found to be eligible, nine of which were conducted in China and the remaining one in Japan. Analysis of eight studies involving dichotomous data revealed that CD147 overexpression in glioma tissue was related to higher WHO grading (III + IV; OR, 9.900; 95 % CI, 5.943, 16.491; P = 0.000) closely, whereas analysis of three studies of continuous data type indicated that there were no statistical associations (standard mean difference, −1.894; 95 % CI, −4.081, 0.293; P = 0.090). In accordance with funnel plot, Egger test, and Begg test, there was no publication bias. Considering that the continuous data make up only a small proportion of the overall analysis, we believe that our study indicates that CD147 overexpression is potentially related to higher WHO grade. Certainly, more data compiled based on evidence-based medicine are required to further support this conclusion.
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Introduction
Histopathologically, gliomas have four WHO grades [1]. Grade I and II gliomas are regarded as low-grade gliomas, and grades III and IV gliomas are high-grade gliomas [2]. The higher the grades, the poorer the prognosis will be. Glioblastoma (grade IV) is the deadliest one. It is important to find more effective biomarkers for the prediction of a glioma’s WHO grade.
CD147, also called tumor cell-derived collagenase stimulatory factor (TCSF), extracellular matrix metalloproteinase inducer (EMMPRIN), or basigin, is a type of neoplasm cell surface molecule that is able to stimulate the surrounding fibroblasts to produce metalloproteinase (MMP) [3]. MMP can degrade extracellular matrix, which is essential for tissue reorganization, tumor’s metastasis, and invasiveness. Previous studies revealed that the expression levels of MMP were much higher during tumor invasion, and CD147 overexpression correlates closely with tumor progression and invasive activity [4]. The role of CD147 in guiding neoplasms’ diagnosis, treatment, and prognosis (such as pancreatic cancer [5], gastric cancer [6], prostate cancer [4], breast cancer [7], and lung cancer [8]), has been widely discussed in recent years. A few studies have also observed its roles in gliomas. They found the CD147 expression might be associated with a glioma’s malignant progression [3, 9–11]. However, the precise role of CD147 in glioma WHO grading is still unclear and remains under discussion. To eliminate the between-study heterogeneity and elucidate a more precise implication of CD147 in gliomas, a meta-analysis was carried out.
Methods
Search Strategy, Study Selection, and Data Extraction
A literature search was performed with Embase, Google Scholar, PubMed, Wanfang, and Cnki databases up to April 2015. Search terms involved “Extracellular Matrix Metalloproteinase (EMMPRIN/CD147)” or “CD147,” “gliomas [MeSH],” “expression,” “grade,” and others. Two reviewers independently selected eligible studies. Disagreement between the two reviewers was settled by discussion with a third reviewer. Inclusion criteria were listed in Table 1. Two reviewers collected the following information independently using a purpose-designed form: author, publication year, country, mean age, patient number, histopathology, test means, WHO grading, and the positive percentage of CD147 in surgical specimen. Disagreement between the two reviewers was resolved by a third one.
Quality Assessment, Data Synthesis, and Analysis
We performed quality assessment of the included studies using European Lung Cancer Working Party (ELCWP) scale [12] (Table 2). Two independent investigators compared their calculated scores and, if necessary, achieved a consensus score for each category during a meeting. Differences were expressed as odds ratios (ORs) or standard mean differences (SMDs) with 95 % confidence intervals (CIs). The Galbraith plot, Cochran’s Q test, and I 2 test (variation in OR attributable to heterogeneity) were all performed to evaluate the heterogeneity between the included studies [13, 14] (Table 2). If there was no evidence of statistical heterogeneity between the studies, then a fixed effect model was used. Otherwise, the random effect model of DerSimonian and Laird was adopted [15]. A sensitivity analysis was performed to examine the stability of the pooled results (Table 2). Potential publication bias was assessed by funnel plot (Table 2) [16] and Egger linear regression test (P < 0.05 indicates publication bias) [17]. Since there were only 10 included studies, meta-regression was not conducted.
P < 0.05 was regarded statistically significant. Data analyses were conducted by means of STATA 12.0 (StataCorp LP, College Station, TX, USA) and Review Manager 5.3 software.
Results
Search Results and Characteristics of the Studies
The article searches were performed as presented in Fig. 1. A total of 10 articles eventually met the inclusion criteria. All 10 studies were done in Asia: 9 were carried out in China and 1 in Japan. The basic characteristics and statistical result of the 10 studies are seen in Tables 3 and 4. The positive rate of CD147 expression varied from 37.68 to 78.69 %. CD147 protein or mRNA in glioma tissues was investigated using immunohistochemistry (IHC; eight studies), RT-PCR (one study), qPCR (one study), and Northern blot (one study). When cytoplasm or nucleus was stained, CD147 was defined as positive.
Study Quality
The detailed ELCWP scores are shown in Table 5. Result analysis achieved the highest mean score of 8.31, followed by designs (8.07), methods (8.06), and generalizability (7.95). The mean global scores for articles in terms of whether only using IHC test methods, countries, whether dichotomous data type or continuous data type showed no significant differences detected by Student’s t test (P > 0.05); this indicated that the baseline characteristics of the various studies did not cause heterogeneity.
Meta-Analysis of CD147 and the WHO Grading System
The WHO grading system for glioma was divided into low (I + II) and high grade (III + IV) for data merging. Information on WHO grading was available in eight studies with dichotomous data and three studies with continuous data (Tables 3 and 4). In the Galbraith plot analysis of dichotomous studies (Fig. 2a), all points fell within the appointed region, which can be taken as evidence of no significant heterogeneity across all of these studies (Q = 7.73, degrees of freedom = 7, I 2 = 9.4 %). As revealed in Fig. 3a, using a fixed effect model, pooled OR indicated a significant association between CD147 expression and high WHO grade (OR, 9.900; 95 % CI, 5.943, 16.491; P = 0.000). This suggested that high CD147 expression in postoperative glioma tissues can predict a high-grade glioma. Conversely, in the Galbraith plot analysis of the continuous studies (Fig. 2b), two points fell outside the region, suggesting relatively high heterogeneity. As shown in Fig. 3b, using a random effect model, the SMDs of the three studies did not indicate that CD147 expression intensity was potently correlated with high-grade gliomas (SMD, −1.894; 95 % CI, −4.081, 0.293; P = 0.090).
Sensitivity Analysis and Publication Bias
The results of sensitivity analysis indicated there are not any individual studies statistically affecting the integrated OR of CD147 and the WHO grade, suggesting that the results were robust (Fig. 4). With Egger test and Begg test, no publication bias was detected among the eight studies that used only IHC in their analyses (P = 0.178; 95 % CI, −0.191, 0.824); similarly, studies using other methods also did not reveal statistical bias (P = 0.560; 95 % CI, −108.60, 95.34). In addition, a funnel plot was overally symmetric (Fig. 5), revealing no significant publication bias.
Discussion
CD147, also known as EMMPRIN, TCSF, or basigin, is a member of the immunoglobulin superfamily. Its structure is related to the putative primordial form of this family [18]; it has been implicated in a variety of physiological and pathological activities. In recent years, studies have demonstrated that CD147 overexpression plays a significant role in the biology of tumors and has an influence on prognosis [5, 18]. One possible explanation for this is its role in producing MMPs, which is a crucial step in tissue remodeling and tumor invasion and metastasis [19]. The number of literatures in relation to CD147 each year shows a general tendency to increase over time. A timeline of the related publications is available as Fig. 6. On the basis of a world map with the global distribution of CD147-related publications based on the analysis of their geolocational data, the countries that the publications are from are mainly concentrated in Europe, North America, and East Asia (Fig. 7). Some studies have reported that CD147 levels were correlated with the progression of malignant glioma. Sameshima proposed that CD147 overexpression in glioma tissues can lead to increased secretion of ECM through interacting with endotheliocytes and fibroblasts, then facilitating cancer cells’ invasiveness and local tissue angiogenesis [3]. Nevertheless, few studies have systematically described the precise clinical implications of CD147 in gliomas and its precise roles in WHO grading. As such, whether CD147 can be used as a biomarker for WHO grading of gliomas remains unclear. In view of this, we combined Embase, Google Scholar, PubMed, Wanfang, and Cnki databases to analyze the clinical significance and practical implications regarding CD147 systematically.
We explored CD147 in 10 glioma literatures and its relation to WHO grading in 615 cases. The analysis of eight dichotomous studies revealed that higher CD147 level was closely correlated with higher pathological grade (III + IV; OR, 9.900; 95 % CI, 5.943, 16.491; P = 0.000), but analysis of the three continuous studies did not detect any statistically associations (SMD, −1.894; 95 % CI, −4.081, 0.293; P = 0.090). However, the later three studies only make up a small part of the overall analysis, so we consider that the present meta-analysis still suggests a potential association between CD147 overexpression and high-grade glioma.
Because there were no significant heterogeneities across the eight literatures with dichotomous data confirmed by Galbraith plot, I 2 test, and Cochran’s Q test, a fixed effect model was chosen. As heterogeneity was detected across the three continuous studies, we adopted a random effect model to conduct the pooled SMD estimates. However, there were still several limitations that should be noted. Publication bias is a major concern in systematic meta-analysis [20]. Most of the journal only published the reports with positive research results. In the “gray literature,” such as meeting monographs, dissertation, etc., there may be potentially “unpublished” negative research results. Unfortunately, although everything possible had been done for searching for these data, nothing gained. Secondly, in this meta-analysis, all the studies are from China and Japan. Frankly, it could lead to selection bias in this regard. But after searching PubMed, Google Scholar, Medline, Cnki, and Wanfang databases, we did only find useable studies from China and Japan. Thirdly, the languages of published eligible articles in this meta-analysis were limited to English and Chinese, which may have caused publication bias because of the absence of studies published in other languages that met our inclusion criteria. It is worth noting here, however, that in the present meta-analysis, neither the Egger’s and Begger’s P value tests (P = 0.178; 95 % CI, −0.191, 0.824) nor the funnel plot indicated publication bias. Thus, at least in statistical terms, this analysis is robust and reasonable.
Conclusions
In conclusion, the present meta-analysis suggests that CD147 is potentially associated with high WHO grading of glioma patients and may be assumed to be an essentially prognostic factor. Most importantly, the pathological CD147 gene or protein detection would facilitate new insights regarding the accurate prediction of tumor grade and early treatment regimens of patients undergoing surgical resection. However, to achieve a more robust conclusion, more data compiled on the basis of evidence-based medicine are required to definitively prove the correlation.
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Kong, X., Wang, Y., Dai, C. et al. Is CD147 a New Biomarker Reflecting Histological Malignancy of Gliomas?. Mol Neurobiol 54, 1568–1576 (2017). https://doi.org/10.1007/s12035-016-9894-2
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DOI: https://doi.org/10.1007/s12035-016-9894-2