Abstract
The BCL-2 family of proteins integrates pro- and anti-apoptotic signals within the cell and is responsible for initiation of caspase-dependent apoptosis. Chronic lymphocytic leukemia (CLL) cells are particularly dependent on the anti-apoptotic protein BCL-2 for their survival, making this an attractive therapeutic target in CLL. Several early efforts to create inhibitors of the anti-apoptotic family members faced significant challenges, but eventually, the BCL-2 specific inhibitor venetoclax moved forward in CLL. Overall and complete response rates to venetoclax monotherapy in relapsed, refractory CLL are approximately 80 and 20%, respectively, even in patients with high-risk 17p deletion. Toxicities have been manageable and include neutropenia, diarrhea, and nausea. The risk of tumor lysis syndrome (TLS), seen in early experience with the drug, has been mitigated by the use of appropriate TLS risk assessment, prophylaxis, and management. Future studies of venetoclax will focus on combination approaches, predictive biomarker discovery, and mechanisms of resistance.
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Introduction
The past decade has seen the emergence of a new class of cancer treatment that operates by directly promoting the cellular process of apoptosis, or programmed cell death. The development of BCL-2 inhibitors began with multiple unsuccessful candidates before finally arriving at venetoclax, which was recently FDA-approved. Here, we review the development of BCL-2 inhibitors and their emergence as a powerful class of agents for the treatment of chronic lymphocytic leukemia (CLL).
Apoptosis and the BCL-2 Family
Apoptosis is an orderly, programmed intracellular signaling cascade that leads to cell death. BCL-2 encodes an anti-apoptotic protein and was the first oncogene identified that primarily exerted its oncogenic effects by inhibiting cell death, rather than by promoting uncontrolled cellular proliferation. Subsequently, additional proteins have been identified that share one or more functional domains with BCL-2. The proteins in this BCL-2 family fall into three groups based on their structure and function (reviewed in [1]). Anti-apoptotic proteins, including BCL-2, MCL-1, BCL-XL, BCL-w, and others, have four tandem BCL-2 homology (BH) domains and ultimately promote cellular survival. Accordingly, their expression is upregulated downstream of pro-survival signaling pathways such as phosphatidylinositol-3-kinase (PI3K) and AKT [2]. Pro-apoptotic BH3-only proteins, including BIM, PUMA, and BAD, have a single BH domain and promote cell death. They are also transcriptional targets of tumor suppressors such as TP53 [3]. The pro-apoptotic effector proteins BAX and BAK are required to carry out the pro-apoptotic effects mediated by other proteins within the family.
BCL-2 family proteins reside on the outer surface of the mitochondrial membrane and function through direct protein-protein interactions with each other, mediated by the BH domain. BH3-only proteins, when not sequestered by anti-apoptotic BCL-2-like family members, can promote the homo-oligomerization of the effector proteins BAX and BAK, leading to mitochondrial outer membrane permeabilization (MOMP), release of cytochrome c, and activation of the caspase cascade [4]. Thus, BCL-2 family members can be thought of as a group of intracellular sensors which detect the net pro- or anti-apoptotic state of the cell, and when the balance tips in favor of apoptosis, they ultimately initiate the irreversible step of MOMP.
This leads to some important conclusions with regard to the clinical relevance of targeting the BCL-2 pathway. First, one method to promote cell death would be to pharmacologically inhibit protein-protein interactions between pro-apoptotic and anti-apoptotic BCL-2 family members, thus freeing pro-apoptotic BH3-only proteins to promote MOMP. Second, directly targeting BCL-2 family members bypasses the need for an intact TP53 signaling axis, as TP53 lies upstream of these proteins.
Functional analysis of a cell’s proximity to the apoptotic threshold (known as “mitochondrial priming”) can be performed through an assay called BH3 profiling [5, 6]. This assay assesses the amount of carefully titrated BH3-only peptides which interact directly with mitochondria to trigger MOMP (defined as release of cytochrome c). BH3 profiling of CLL cells indicates that these cells are generally highly primed for apoptosis [7]. Supporting this finding, BCL-2 protein expression levels are typically higher in CLL cells than in peripheral blood mononuclear cells. Additionally, BCL-2 sequesters large amounts of the pro-apoptotic BH3-only protein BIM, causing CLL cells to be highly dependent on BCL-2 for survival [7]. Although CLL cells depend on BCL-2 for survival, they have not been found to harbor genetic mutations or translocations of BCL-2 family members. This mirrors the functional dependence of CLL cells on the B cell receptor (BCR) signaling pathway, where activating mutations of key proteins such as Bruton’s tyrosine kinase (BTK) are also not present [8].
Early Attempts at Targeting BCL-2 in the Clinic
Oblimersen
Oblimersen is an antisense oligodeoxynucleotide designed to target BCL-2 mRNA [9]. It was found to decrease BCL-2 expression and the proliferation rate of lymphoma cell lines [10]. A phase I/II study in relapsed/refractory CLL identified a cytokine-release syndrome, consisting of fevers and hypotension, as the dose-limiting toxicity. Efficacy as a single agent was modest, with only 2 of 26 evaluable patients (8%) having a partial response [11]. The drug was later tested in a phase III trial, with patients randomized to fludarabine/cyclophosphamide or fludarabine/cyclophosphamide/oblimersen. There was no significant difference in the 5-year survival rate between the two arms in an intent-to-treat analysis [12], and further development of this agent in CLL was halted.
Gossypol Compounds
Gossypol is a polyphenolic aldehyde isolated from the cotton plant. Gossypol directly interacts with BCL-XL and displaces BH3 proteins with an IC50 of 0.5 μM [13]; subsequent derivatives of gossypol interact with a spectrum of anti-apoptotic BCL-2 family members with submicromolar affinities [14, 15]. Gossypol treatment of primary lymphocytes taken from CLL patients induces caspase-independent apoptosis [16]. AT-101 (“apo-gossypol”) is an enantiomer of gossypol (R(-)-gossypol) that binds BCL-2, BCL-XL, and MCL-1 and induces apoptosis in primary CLL cells at concentrations of 10–30 μM [17]. A phase I trial of AT-101 monotherapy in patients with relapsed/refractory CLL identified transaminitis as a major toxicity, with preliminary results indicating limited efficacy [18]. A subsequent phase II study of AT-101 in combination with rituximab for relapsed/refractory CLL treatment reported a preliminary overall response rate (ORR) of 44% with no complete responses [19].
Obatoclax
Obatoclax (GX15-070) is a small molecule identified in a screen for compounds that specifically disrupt protein-protein interactions between BCL-2 family members [20]. It is considered a “pan-BCL-2” family inhibitor, with the ability to bind BCL-2, BCL-XL, BCL-w, and MCL-1 at concentrations of 1–7 μM [20] and to induce apoptosis in primary CLL cells in vitro [21]. Obatoclax-induced cell death, however, is only partially abrogated by deletion of BAX and BAK, suggesting that the drug likely also causes cytotoxicity through mechanisms other than MOMP and apoptosis [22]. Phase I studies of obatoclax in CLL patients demonstrated significant toxicities with limited efficacy. Neurologic symptoms such as somnolence, ataxia, and confusion were found to be dose-limiting [23]. Only 1/26 patients (4%) in the phase I trial of obtatoclax monotherapy experienced a partial response [23]; a phase I trial of obatoclax in combination with fludarabine and rituximab for relapsed/refractory CLL had a partial response rate of 54% [24]. The toxicities of obatoclax, along with its limited efficacy compared to navitoclax and venetoclax, ultimately limited its development as a therapeutic agent for CLL.
In summary, despite some promising pre-clinical data, multiple early attempts to inhibit BCL-2 family members in patients were largely unsuccessful. Given the compelling biology of BCL-2 dependence in the pathophysiology of CLL, this lack of early success did not dissuade investigators from pursuing BCL-2 as a therapeutic target.
More Recent Attempts at Targeting BCL-2 in the Clinic
Navitoclax
A breakthrough in the development of BCL-2 inhibitors occurred through a screen for small molecules that block the hydrophobic BH3-binding domain of BCL-XL [25], which eventually identified ABT-737, which binds to BCL-2, BCL-XL, and BCL-w with high affinity (Ki ≤ 1nM). This binding disrupts their interactions with pro-apoptotic BH3-only family members, which are then free to bind to BAX/BAK, leading to oligomerization and MOMP. Navitoclax (ABT-263) is a second generation, structurally related molecule that is orally available and has more favorable pharmacokinetics [26]. It has an oral bioavailability of 20–50% and a half-life of 8.9 h [26], making it suitable for once-daily dosing. Its specificity mirrors that of ABT-737, with a Ki of ≤1 nM against BCL-2, BCL-XL, and BCL-w, and a Ki of 550 nM against MCL-1 [26].
Promising pre-clinical findings [26, 27] led to the development of clinical trials in lymphoid malignancies. In a phase I trial of navitoclax in 55 patients with a variety of lymphoid malignancies, the subset of 20 patients with CLL/SLL were found to be particularly responsive to the drug, with a median progression-free survival (PFS) of 246 days [28]. Subsequently, a phase I study of navitoclax restricted to patients with relapsed/refractory CLL was undertaken [29]. Nine out of 29 patients (31%) achieved a partial response, and 90% of patients had at least a 50% reduction in their peripheral blood lymphocyte count. Notably, responses were fairly durable, with a median PFS of 25 months in a heavily pretreated group of patients. An open-label, randomized phase II study compared navitoclax plus rituximab to rituximab alone in previously untreated CLL. The addition of rituximab to 12 weeks of navitoclax led to an ORR of 55%, compared to 35% for patients treated with rituximab monotherapy. The combination of rituximab with navitoclax given until time of progression further increased the ORR to 70% [30].
The dose-limiting toxicity of navitoclax was a dose-dependent reduction in platelet count, with grade ≥3 thrombocytopenia (platelet count <50,000) occurring in 28% of patients in the phase I CLL study [29] and 26% of patients in a phase II study [30]. This was attributed to BCL-XL inhibition in platelets [31], and prompted a drive to identify an inhibitor that retained activity against BCL-2 but lacked activity against BCL-XL.
Venetoclax
Venetoclax (ABT-199/GDC-0199) is the result of reverse engineering of navitoclax to increase BCL-2 selectivity [32••] (Fig. 1). Accordingly, venetoclax has subnanomolar affinity for BCL-2 (Ki < 0.010 nM), but significantly weaker binding to BCL-XL (Ki = 48 nM), BCL-w (Ki = 245 nM), and MCL-1 (Ki > 444 nM) [32••]. Venetoclax has adequate oral bioavailability and an estimated half-life of 26 h [33, 34]. Consistent with the known BCL-2 dependence of CLL cells, venetoclax treatment in vitro induced apoptosis in primary CLL cells, with a remarkable EC50 of 3 nM [32••].
Venetoclax First-in-Human Study
A first-in-human phase I study of venetoclax was initiated in 2011, with initial dosing ranging from 100 to 200 mg. Of the first three patients treated, the two with peripheral lymphocytosis experienced a rapid reduction in the white blood cell count within 8 h of the first dose. All three patients developed laboratory evidence of tumor lysis syndrome (TLS) without clinical sequelae, and all three patients were able to safely resume dosing after resolution of TLS [32••].
Due to the TLS observed in these first three patients, the dosing regimen on this study was modified to include a lower starting dose of 50 mg as well as a weekly intrapatient dose ramp-up to a target dose for each dosing cohort, up to a maximum dose of 1200 mg daily. The multicenter, open-label phase 1 study ultimately enrolled 116 patients with relapsed or refractory CLL or SLL [35••]. Patients received daily venetoclax until disease progression or unacceptable toxicity. Despite the initial revision to start at a lower dose of 50 mg, there were two clinically significant cases of TLS (one case resulting in the need for hemodialysis and one fatality). This led to further revision of the dosing scheme, with venetoclax beginning at 20 mg followed by weekly intrapatient dose ramp-up over 5 weeks to 50, 100, 200, and finally 400 mg per day. Patients were monitored closely for TLS, including hospitalization for those with a high burden of disease, and supportive measures such as intravenous hydration and uric acid-lowering agents were mandated. Notably, none of the patients treated with this most recent dosing regimen experienced clinical TLS. Neutropenia (in 45% of patients, with 41% grade ≥3) and gastrointestinal side effects (diarrhea in 52% of patients, with 2% grade ≥3; nausea in 47% of patients, with 2% grade ≥3) were common adverse events. The drug was highly efficacious as monotherapy, even in high-risk patients such as those with del(17p) and those refractory to fludarabine-based chemoimmunotherapy. The ORR in all patients, including both the dose-escalation cohort and the expansion cohort, was 79%. In the subgroup of patients with a 17p deletion, the ORR remained high at 71%, and in the subgroup of patients refractory to fludarabine, the ORR was 79%. However, while the ORR in the high-risk 17p-deleted subgroup of patients was similar to the overall cohort, this cohort did have a significantly shorter PFS than the non-17p-deleted cohort. Complete responses (CRs) by IW-CLL criteria [36] were seen in 20% of patients (with comparable CR rates in the high-risk subgroups), and 5% of patients achieved minimal residual disease (MRD) negativity. A report with longer follow-up on the cohort of patients who received the recommended phase II dose of 400 mg/day was notable for an ORR of 81% (CR rate of 16%) and also highlighted the durability of responses, with an estimated 24-month PFS of 62% [37].
Venetoclax in del(17p) CLL
The success of venetoclax monotherapy in the 17p-deleted population was later confirmed in a landmark phase II, single-arm, open-label, multicenter study in 107 subjects receiving venetoclax 400 mg daily for relapsed, refractory, 17p-deleted CLL [38•]. Subjects continued venetoclax until disease progression or unacceptable toxicity. To reduce the risk of TLS, a stepwise ramp-up dosing strategy was again used. The ORR was 79% with an 8% CR rate. With a median duration of follow-up of only 12 months, it is possible that the CR rate may increase over time based on experience from the phase I study. In April 2016, venetoclax received FDA approval for the treatment of patients with 17p-deleted CLL who have relapsed after or are refractory to at least one prior line of therapy.
Venetoclax for Patients Who Progress on BCR Pathway Inhibitors
Venetoclax has a distinct mechanism of action from the B cell receptor (BCR) pathway inhibitors such as the BTK inhibitor ibrutinib and the PI3K-δ inhibitor idelalisib. Thus, one would hypothesize that venetoclax may be effective in patients who have progressed on these kinase inhibitors. This hypothesis is being tested in a phase II study of venetoclax in patients who have CLL that is relapsed after, or refractory to, ibrutinib or idelalisib. Venetoclax monotherapy is dosed as per the latest schedule from the prior trials, and it is continued until disease progression. Early results from 64 patients have been presented in abstract form [39], with an ORR of 70% in patients who progressed on ibrutinib and 48% in patients who progressed on idelalisib. Several CRs were seen despite the short follow-up.
Venetoclax Combination Studies
While venetoclax clearly has efficacy in CLL as monotherapy, there is also good justification for using it as a component of combination therapy. Its mechanism of action and toxicity profile are different from those of other approved agents for CLL, and combination therapy may be able to achieve deeper, more durable responses than venetoclax alone.
Venetoclax may synergistically enhance antibody-dependent cytotoxicity [40], and thus, some initial studies have explored the combination with anti-CD20 monoclonal antibodies. In results from a phase Ib, open-label, multicenter study of venetoclax plus rituximab in 49 patients, the ORR was 86% [41••], with 51% of patients achieving CR or CRi after a median 28 months of follow-up, exceeding the CR rates seen with monotherapy (although patients on this combination were less heavily pre-treated than those on the monotherapy studies). Eighty percent of patients with CR/CRi had no minimal residual disease (MRD) by ≥4 color flow cytometry on bone marrow biopsy. The toxicity profile, including neutropenia (55%), diarrhea (57%), and nausea (51%), was similar to that seen with venetoclax alone. There was one fatal TLS event prior to a protocol modification specifying parameters for TLS risk management and prophylaxis. Based on the results of this trial, venetoclax was given breakthrough therapy designation by the FDA in combination with rituximab for the treatment of relapsed/refractory CLL. Venetoclax, bendamustine, and rituximab triplet combination therapy is being investigated in a phase I study in both relapsed/refractory and untreated CLL; preliminary results showed an ORR of 96% in 30 patients with relapsed/refractory disease [42]. In similar fashion, venetoclax has been combined with obinutuzumab in a currently ongoing phase Ib study in both relapsed/refractory as well as untreated CLL [43]. In the 17 enrolled patients with relapsed/refractory disease, the preliminary ORR is 100%, with a CR/CRi rate of 23.5%.
These early-phase clinical trial findings will need to be confirmed in large, randomized trials. The MURANO trial is a phase III study randomizing patients with relapsed/refractory CLL to either 6 cycles of venetoclax/rituximab therapy (followed by venetoclax maintenance therapy for up to 24 months) or 6 cycles of bendamustine/rituximab. It has completed enrollment, but results have not yet been reported. The CLL14 trial, a phase III study of venetoclax/obinutuzumab versus chlorambucil/obinutuzumab in previously untreated older patients with CLL, is also now fully accrued and, if positive, could help define a new standard of care for these patients [44].
Additional trials have recently launched to study an all-oral regimen of venetoclax in combination with ibrutinib. Using BH3 profiling, ibrutinib has been shown to enhance ex vivo CLL cell sensitivity to venetoclax, in part, by upregulating the proapoptotic BIM protein [45] and decreasing expression of BCL-XL and MCL-1 [46]. A non-randomized, phase II study of ibrutinib in combination with venetoclax (NCT02756897) is currently enrolling patients, and two phase II trials have also recently launched to examine the three drug combination of ibrutinib, venetoclax, and obinutuzumab in previously untreated patients with 17p-deleted CLL (NCT02758665) and in previously untreated or relapsed/refractory patients in any cytogenetic risk group (NCT02427451). A summary of the trials of venetoclax in CLL can be found in Table 1.
Safely Administering Venetoclax
The numerous clinical trials investigating the role of venetoclax in the treatment of CLL have provided initial insight into its toxicity profile. The dose-dependent thrombocytopenia seen with navitoclax has not been observed with venetoclax. TLS has been the most concerning adverse event observed, and optimal TLS prophylaxis requires both careful assessment and management of risk. TLS risk stratification requires assessment of disease burden in the peripheral blood and lymph nodes. Low risk patients have no lymph nodes ≥5 cm in diameter and have an absolute lymphocyte count <25 × 109 cells/L, high-risk patients meet both of these criteria (or have any lymph node ≥10 cm in size), and medium-risk patients meet only one of these criteria. Patients with pre-existing renal impairment should also be considered high risk. Regardless of risk category, patients initiating venetoclax should receive allopurinol, aggressive oral or intravenous hydration, and laboratory monitoring for TLS. Additionally, high-risk patients should receive their initial doses of venetoclax in an inpatient setting, with prophylactic rasburicase and even more intensive laboratory monitoring. Utilizing this approach, the rate of laboratory TLS was 4% in a recent integrated safety analysis of nearly 300 patients treated on three clinical trials with venetoclax monotherapy [48]. All of these events occurred during the ramp-up phase of venetoclax dosing, and none resulted in clinical sequelae.
One of the most frequent adverse events with venetoclax monotherapy is neutropenia, with an incidence of 38% (and 36% for grade ≥3 neutropenia [absolute neutrophil count <1000 cells/μL]) [48]. The presumed mechanism is due to on-target BCL-2 inhibition in neutrophil precursors. The incidence of febrile neutropenia has been low at approximately 5% [48] and can be managed safely with growth factor support given concomitantly with venetoclax. Less commonly, venetoclax dose interruption or dose reduction may be required. Outside of effects on neutrophils, the impact of venetoclax on the immune system seems modest, with negligible impact on T cells, NK cells, or immunoglobulin levels [37]. Gastrointestinal side effects—such as nausea, vomiting, constipation, and diarrhea—impact approximately half of all patients receiving the drug, but are generally low-grade and transient.
Unanswered Questions with BCL-2 Inhibition
The clinical experience with BCL-2 inhibitors, particularly venetoclax, has prompted some interesting yet currently unanswered questions. For example, recent data have suggested that patients who achieve MRD-negativity with chemoimmunotherapy, either with a CR or a PR, have superior PFS and overall survival (OS) compared to patients whose best response is MRD-positive, even if they are otherwise in a CR [49]. One important question is whether achieving MRD-negativity with venetoclax is also predictive of a longer PFS and OS. Compared to BTK inhibitors, which have a CR/CRi rate of 0–7% in relapsed/refractory patients [50, 51], venetoclax appears to be superior at achieving CRs, in many cases with MRD-negativity. If achievement of MRD-negativity with venetoclax can be validated as predictive of a long-term disease-free state, both patient care and future trial design would be greatly impacted. This would suggest that venetoclax would be an excellent backbone upon which future front-line combination regimens can be built.
Closely related to the issue of achieving MRD negativity is the unresolved question regarding the optimal dosing duration for venetoclax. Should venetoclax be continued until progression, or can it be stopped after a fixed period of time (particularly if the patient has achieved MRD negativity)? The phase Ib trial of venetoclax plus rituximab gives subjects the option of discontinuing therapy after achieving a CR/CRi (a subsequent amendment also required MRD negativity in the bone marrow at time of treatment cessation). Thirteen subjects discontinued therapy. Only two of the 13 (both of whom were MRD positive) had relapsed after a median of 9.7 months off all therapy. Both of these subjects then restarted venetoclax and achieved a partial response [41••]. These preliminary data suggest that venetoclax can be discontinued in some patients and retreatment can be effective, but larger numbers are needed to confirm this strategy. Additional studies of venetoclax with a fixed duration of therapy will provide further insight into this question.
Another key issue with venetoclax is whether predictive biomarkers for response can be identified. A recent study of samples taken from venetoclax-treated patients showed that neither TP53 status nor in vitro sensitivity of CLL cells to the drug predicted depth of response to venetoclax in patients. However, BH3 profiling was able to significantly identify the subset of patients who had a greater reduction in CLL bone marrow infiltrate [52•]. Further validation of these initial findings will require systematic evaluation of a larger cohort of patients.
Another critical issue is elucidating mechanisms of resistance to BCL-2 inhibition. Preclinically, there is evidence to suggest that upregulation of alternative anti-apoptotic BCL-2 family members may lead to BCL-2 inhibitor resistance. For example, one group used stromal co-culture of CLL cells to mimic the lymph node microenvironment, and observed increased expression of BCL-XL and BCL2A1, which correlated with a change in the EC50 of ABT-737 from 7 to >10 μM [53]. Another group treated seven lymphoma and leukemia cell lines in long-term culture with steadily increasing concentrations of venetoclax, eventually generating clones that had 100-fold less sensitivity to the drug [54]. The clones displayed reductions in the proapoptotic proteins BAX, BIM, and NOXA and increases in BCL-XL and MCL-1. These findings were recapitulated by a second group which demonstrated upregulation of BCL-XL and MCL-1 in lymphoma cell lines that became resistant to venetoclax after chronic exposure [55]. Mutations in BCL-2 that block venetoclax binding, and an inactivating mutation in BAX that blocks the protein’s ability to attach to the outer mitochondrial membrane, have also been described in cell line models of acquired venetoclax resistance [56]. Clinically, a significant number of patients who have progressed on venetoclax have done so through Richter’s transformation, which accounted for 44% of progression events in the phase I first-in-human study of venetoclax monotherapy [35••]. As this phenomenon has also been observed in CLL patients treated with other novel agents such as ibrutinib, these transformation events may reflect the fact that novel agents allow patients with genetically unstable (e.g., TP53 deleted) disease to live longer, providing more time for transformation to occur, rather than inducing transformation directly.
Conclusions
The development of BCL-2 inhibitors for use in CLL represents a triumph of rational scientific investigation. While initial attempts at BCL-2 family inhibition did not achieve the desired specificity and efficacy, rational drug design eventually yielded a viable approach with BH3 mimetics. And while the early experience with navitoclax was hampered by the unanticipated development of thrombocytopenia, this problem was overcome with the BCL-2 specific inhibitor venetoclax, an agent so effective in CLL that its most serious toxicity is TLS due to rapid tumor cell killing. The task now turns to clinical investigators to identify the best way to incorporate venetoclax into the expanding arsenal of drugs for the treatment of CLL. Future studies in CLL will focus on venetoclax combination therapies, timing and duration of venetoclax dosing, predictors of response, and mechanisms of resistance. While many unanswered questions yet remain, the future of BCL-2 inhibition in CLL treatment is bright.
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Benjamin L. Lampson declares no potential conflicts of interest.
Matthew S. Davids reports grants from Infinity, Genetech, Pharmacyclics, and TG Therapeutics and personal fees from Infinity, Genetech, Gilead, Janssen, Pharmarcyclics, TG Therapeutics, Celgene, and Abbvie.
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Lampson, B.L., Davids, M.S. The Development and Current Use of BCL-2 Inhibitors for the Treatment of Chronic Lymphocytic Leukemia. Curr Hematol Malig Rep 12, 11–19 (2017). https://doi.org/10.1007/s11899-017-0359-0
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DOI: https://doi.org/10.1007/s11899-017-0359-0