Abstract
The endophytic bacterium, Pseudomonas aeruginosa, was successfully encapsulated into calcium carbonate microshells and coated with sodium alginate in combination with two other materials: skim milk and empty fruit bunch (EFB). The presence of bacteria cells was confirmed by a strand-like structure, a biofilm through morphology and elemental analysis. The survivability of microencapsulated bacteria was found to last for 17 months when they were maintained in a storage condition of 4 °C. Different coating materials used exhibited significant differences in the P. aeruginosa survival during the storage time. Their bioactivity against Ganoderma boninense resulted in a percentage inhibition radial growth (PIRG) value of more than 70%, which is better than its counterparts, the free Pseudomonas cells. With promising viability results of × 106 CFU/mL after three-month storage, the results demonstrate that skim milk-coated alginate might be good protection for P. aeruginosa that could sustain the viable bacteria at the target site. This is toward a greener, biological control-based plant protection for Ganoderma diseases in the oil palm planting industry.
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Acknowledgements
The authors gratefully acknowledge Universiti Putra Malaysia and the Ministry of Higher Education of Malaysia (UPM/MOHE) grants under the LRGS-NANOMITE, Vote no. 9443100 and 5526300 for the funding. The experiment and dual culture study were performed at the Malaysian Palm Oil Board (MPOB) laboratories and facilities.
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Mustafa, I.F., Hussein, M.Z., Idris, A.S. et al. Pseudomonas aeruginosa encapsulated with calcium carbonate microshells for potential biocontrol of the Ganoderma boninense. Korean J. Chem. Eng. 40, 854–862 (2023). https://doi.org/10.1007/s11814-022-1351-5
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DOI: https://doi.org/10.1007/s11814-022-1351-5