Abstract
The performance of various potato cultivars in response to disease pressures from Meloidogyne incognita (MI) and Ralstonia solanacearum (RS) is believed to be different. The experiment was laid out in a randomised complete block design (RCBD) with three replications. A total of 13 potato cultivars during the main cropping season were assessed. The mean squares values from the analyses of variance for MI, RS, and plant parameters of potato cultivars at the two hot spot sites, ‘Kersa’ and ‘Arbarakate’ for the two pathogens showed highly significant (P < 0.01) differences among cultivars in terms of response to pathogens and plant parameters. At ‘Kersa’, all of the tested potato cultivars were classed as ‘moderately resistant’. However, ‘Gudenie’ and ‘Belete’ were classed as ‘resistant’ to RS. At ‘Arbarakate’, ‘Belete’, and ‘Bubu’ were classed as ‘resistant’ to MI, while ‘Gudenie’, ‘Belete’, and ‘Bubu’ were classed as ‘resistant’ to RS. At ‘Kersa’, ‘Gudenie’ recorded the highest mean values (25.5 t ha−1) of marketable tuber yield (MY) and total yield (TY) (39.2 t ha−1). At ‘Arbarakate’, the highest mean value (49.6 t ha−1) of TY was registered from the cultivar ‘Bubu’. TY had a negative phenotypic correlation with the pathogen’s parameters but a positive with plant parameters. ‘Gera’ was the most distant from all tested cultivars with Euclidean distance = 30.8. These assessments provide information for breeders for further improvement through selection.
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Introduction
Potato (Solanum tuberosum L.) cultivars show resistance to some diseases and pests (Barrell et al. 2013); pests and diseases, such as the root-knot nematode (RKN; Meloidogyne spp.), still cause significant, up to 100%, yield losses, particularly in vegetable crop production (Onkendi et al. 2014; Seid et al. 2015). In Ethiopia, the occurrence of RKN has been reported on a small number of horticultural crops (Tefera and Hulluka 2000; Mandefro and Mekete 2002; Abegaz et al. 2019; Kassie 2019; Miheret et al. 2019; Seid et al. 2019). Ralstonia solanacearum, cause of bacterial wilt of Solanaceae crops, is another globally important destructive potato disease (EPPO 2020). Previously, the performances of various released and local potato cultivars in terms of plant yield and related parameters have been reported in Ethiopia (Berhanu and Tewodros 2016; Habtamu et al. 2016; Wassu 2016; Tessema et al. 2020). However, there is limited information on their performance in the presence of RKN and bacterial wilt disease complex. Crop losses are reported to be aggravated when RKN is found in association with bacterial wilt (Bekhiet et al. 2010; Shahbaz et al. 2015; Sundaresh et al. 2017).
The potato cultivars tested at different locations for resistance to the two diseases are believed to have genotypic and phenotypic correlation, genetic distance, and heritability differences. Estimates of heritability for different characters provide a picture of the amount of heritable variation present in different parameters (Johnson et al. 1955). The experiment was carried out to study the extent of association of genetic variability in potato cultivars for yield-related parameters and to find out the best cultivars for further use by farmers and in breeding programmes.
There is limited research information on the evaluation of potato cultivars’ reaction to RKN and bacterial wilt disease complex at field conditions. The experiment aimed at evaluating potato cultivars for the two diseases helps to study the extent of association of genetic variability in potato cultivars for yield-related parameters and to find out the best cultivars for further use by farmers and in breeding programmes. The objectives of the present study were, therefore, to evaluate tuber yield performance, identify potential resistant potato cultivars to Meloidogyne incognita and Ralstonia solanacearum diseases complex, and group cultivars into different clusters to determine association yield, yield-related traits, and disease parameters.
Materials and Methods
Description of the Study Sites
Two farmer’s fields that are known as hot spots for RKN and RS were selected based on the information generated from the survey conducted during the 2018 main cropping season. ‘Kersa’ is located at an altitude of 1990 m.a.s.l., 09°15″N latitude, and 41°40″E longitude (SEHZOR 2006). The area is characterised by annual minimum and maximum temperatures of 12 and 24 °C, respectively, and receives 780 mm of annual rainfall (EMA 2011). ‘Arbarakate’ is located at an altitude of 2280 m.a.s.l., 9°14″N latitude, and 41°2″E longitude (SEHZOR 2006). The area is characterised by annual minimum and maximum temperatures of 12 and 23 °C, respectively, and receives 1150 mm of annual rainfall (EMA 2011).
Experimental Materials
A total of 13 potato cultivars recommended for cultivation under different agroecologies of the country between 1998 and 2013 were assessed. The cultivars were tested to be free from viral, wilt, and other plant diseases, and seed tubers were obtained from the Amhara Region Agricultural Research Institute (ARARI). The potato cultivars used in this study are shown in Table 1.
Experimental Procedures and Design
The experimental fields were prepared with a tractor to a depth of 25–30 cm, and ridges were prepared by hand. Plots measuring 4.5 m × 3.6 m consisting of six rows that could accommodate twelve plants at a spacing of 0.75 m between ridges and 0.30 m between plants, with spacing between plots and adjacent replicates of 1 and 1.5 m, respectively, were prepared for planting the potato cultivars during the main cropping season of 2020. Sprouted tubers measuring about 39 g were planted at the sides of ridges and at a depth of approximately 5 to 10 cm. The recommended rates of phosphorus at the rate of 100 kg P2O5 per ha in the form of diammonium phosphate were used, and the whole rate was applied (10 cm below the seed tuber) at planting. Nitrogen at the rate of 150 kg per ha was applied (7 to 10 cm away from the plant) in the form of urea in two splits: half rate after full emergence (2 weeks after planting) and half rate at the initiation of tubers (start of flowering). Weeding and other agronomic practices were performed as per normal crop management practices. The cultivars were harvested when the plants reached physiological maturity, as shown by yellowing or senescence on the lower leaves. The experiment was laid out in a randomised complete block design (RCBD) with three replications.
Nematode Identification
From each row, 90 days after planting, 10 g root system and 10 g tuber skin were collected, and female root-knot nematodes were stained with 1 ml acid fuchsine solution (3.5 g acid fuchsine/250 ml acetic acid and 750 ml distilled water) and then dislodged with a needle. The posterior portion of the female nematode was cut with a knife. The body contents were cleaned. The cleaned posterior portion was trimmed and transferred to a drop of glycerine on a clean microscopic slide and then observed under a stereomicroscope. M. incognita was identified from other Meloidogyne spp. based on the perennial pattern described by Taylor and Sasser (1978) and the morphology of the adult females (Eisenback and Hirschmann 1981).
Data Collection
The number of galls per root system and tuber (G/R) and the root system and tuber gall index (RGI) were recorded 90 days after planting. Root-gall index (RGI) was determined as described by Taylor and Sasser (1978). Resistance/susceptibility of the cultivars to RKN was scored using ratings depicted by Pederson and Windham (1989). RGI = [∑ (Si × Ni) ÷ (N × 5)] × 100; where Si is root and tuber galling scale of 0, 1, 2, 3, 4, 5, where 0 = no galls; 1 = 1 or 2, 2 = 3–10; 3 = 11–30; 4 = 31–100; 5 > 100. Ni is the number of plants in each root and tuber galling scale. Nis the total number of evaluated plants. From these figures, the resistance/susceptibility was scored using the following system: Immune RGI = 0; highly resistant 0.1 ≤ RGI ≤ 5.0; resistant 5.1 ≤ RGI ≤ 25.0; moderately susceptible 25.1 ≤ RGI ≤ 50.0; susceptible 50.1 ≤ RGI ≤ 75.0; highly susceptible RGI > 75.0. Root system and tuber were rated for galling severity on a 0 to 4 scale, where 0 = no galling (0%), 1 = light galling (1–25%), 2 = moderate galling (26–50%), 3 = heavy galling (51–75%), 4 = severe galling (76–100% galled root system and tuber) according to Barker (1985).
Resistance/susceptibility of the cultivars to bacterial wilt was scored using indices described by Winstead and Kelman (1952). Bacterial wilt index (BWI) = ∑ (ni × vi) ÷ (V × N); where the ni = number of plants with the respective disease rating; vi = disease rating: 0 = no wilting, 1 = < 10% wilted plants, 2 = 11–25% wilted plants, 3 = 26–50% wilted plants, 4 = 51–75% wilted plants, 5 = > 75% wilted plants; V = the highest disease rating (5); N = the number of plants observed. Highly resistant (BWI = 0.0–0.2), resistant (BWI = 0.2–0.3), moderately resistant (BWI = 0.31–0.4), moderately susceptible (BWI = 0.41–0.5), susceptible (BWI = 0.51–0.60), highly susceptible (BWI = 0.61–0.9), extremely susceptible (BWI = 0.91–1.0).
Marketable tuber number per plant (MTNPP), unmarketable tuber number per plant (UMTNPP), average tuber weight (ATW in g), marketable yield (MY; number of MTNPP * ATW/plot area), unmarketable yield (UMY), and total tuber yield (TY in t ha−1)) were recorded 90 days after planting.
Data Analysis
All the M. incognita, Ralstonia solanacearum, and plant-related data from each location were subjected to analysis of variance (ANOVA) using RCBD. The error variance homogeneity test was conducted using F-ratio, before the combined ANOVA over locations was conducted for each parameter. Some of the data were transformed using log (x + 1). The mean performances of cultivars were compared based on pooled means over locations using Duncan multiple range test (DMRT) at P ≤ 0.05 depending on the results of each location and over-location ANOVA and error variance homogeneity test. All analyses were computed using SAS software version 9.2.
The genetic distance of potato cultivars was estimated using Euclidean distance (ED) calculated from data collected from field experiments after standardisation (subtracting the mean value and dividing it by the standard deviation) as established by Sneath and Sokal (1973) as follows:
EDjk = distance between cultivars j and k; Xij and Xik = pathogens and plant-related parameter values of the ith character for cultivars j and k, respectively; n = number of parameters used to calculate the distance. The distance matrix from pathogen and plant-related parameters was used to construct a dendrogram based on the unweighted pair-group method with arithmetic means. The results of cluster analysis were presented in the form of a dendrogram.
Phenotypic and Genotypic Correlation Coefficients
Phenotypic (rp) and genotypic (rg) correlations between two parameters were estimated using the formula suggested by Johnson et al. (1955); Singh and Chaudhry (1985).
\(rpxy\) = phenotypic correlation coefficient between character x and y; \(COVpxy\) = phenotypic covariance between character x and y; \({\sigma }^{2}px\) = phenotypic variance for character x; \({\sigma }^{2}py\) = phenotypic variance for character y.
where \(rgxy\) = genotypic correlation coefficient between character x and y; \(COVgxy\) = genotypic covariance between character x and y; \({\sigma }^{2}gx\) = genotypic variance for character x; \({\sigma }^{2}gy\) = genotypic variance for character y. The coefficient of correlation at the phenotypic level was tested for significance by comparing the values of correlation coefficient (r) with tabulated r-value at g ˗ 2 degrees of freedom, where g is a number of genotypes/cultivars. However, the coefficient of correlations at the genotypic level was tested for significance using the formula described by Robertson (1959).
The calculated t value was compared with the tabulated t value at g ˗ 2 degrees of freedom at a 5% level of significance, where g = number of genotypes, rgxy = genotypic correlation coefficient, and SErgxy = standard error of genotypic correlation coefficient between character x and y, which were calculated as
H2x = heritability value of character x, and H2y = heritability value of character y. Broad sense heritability (H2b) was estimated by the formula suggested by Johnson et al. (1955). Low (0–30%), medium (31–60%), and high (61% and above). H2b = (σ2g/σ2p)*100; where σ2g = genotypic variance and σ2p = phenotypic variance.
Results
Mean Performance of the Potato Cultivars
The mean square values for Meloidogyne incognita (MI), Ralstonia solanacearum (RS), and plant-related parameters/traits of potato cultivars at ‘Kersa’ and ‘Arbarakate’ showed highly significant (P < 0.01) differences among cultivars on the number of galls per root system and tuber (G/R), root and tuber gall index (RGI), and bacterial wilt index (BWI). It also showed highly significant differences in marketable tuber number per plant (MTNPP), unmarketable tuber number per plant (UMTNPP), average tuber weight (ATW), marketable yield (MY), unmarketable yield (UMY), and total yield (TY), except RGI and ATW in ‘Kersa’, which showed nonsignificant differences.
The mean squares from combined analysis of variance for MI, RS, and plant parameters of cultivars tested at the two locations revealed the presence of highly significant differences among the cultivars, except for parameter RGI, which showed nonsignificant differences. It also exhibited the absence of a significant difference between the locations except for BWI. However, the cultivar and location interactions showed highly significant differences in BWI and UMTNPP (Table 2). At ‘Kersa’,‘Gudenie’, ‘Belete’, and ‘Bubu’ showed the lowest mean values of G/R (19.3, 16.3, and 16) and RGI each 3.0, respectively, but ‘Shonkolla’ had the highest G/R (35.3) and RGI (4.0), respectively. Among all the tested cultivars, ‘Belete’ registered the lowest mean values (0.23) of BWI, but ‘Shonkolla’ had the highest mean values (0.83) of the parameter.
At ‘Arbarakate’, ‘Bubu’ registered the lowest G/R (9.3) and RGI (2.3), but ‘Jalenie’ had the highest G/R (27.3). At this location, ‘Belete’ showed the lowest (0.20), while ‘Gera’ showed the highest mean value (0.57) of BWI (Table 3).
At ‘Kersa’, the galling severity of all cultivars ranged from light galling to moderate galling. ‘Gudenie’, ‘Mara Charre’, ‘Belete’, and ‘Bubu’ showed light galling. The other cultivars all showed moderate galling. None of the tested cultivars was ‘resistant’ to MI in this location. ‘Chiro’ was classed as ‘moderately susceptible’ while the others were classed as ‘moderately resistant’. Only ‘Gudenie’ and ‘Belete’ were classed as ‘resistant’ to RS. The other cultivars categorised were classed as ‘moderately susceptible’ to ‘highly susceptible’.
At ‘Arbarakate’, the severity of the galls seen on all cultivars was classed as light galling. ‘Belete’ and ‘Bubu’ were classed as ‘resistant’, while the other cultivars were classed as ‘moderately resistant’ to the MI. Only ‘Gudenie’, ‘Belete’, and ‘Bubu’ were classed as ‘resistant’ to RS, while ‘Gera’ and ‘Araarsaa’ were ‘susceptible’. The other cultivars were classed as ‘moderately susceptible’ to RS (Table 4).
At ‘Kersa’, ‘Gudenie’ and ‘Guassa’ produced the highest mean value (9.3) of MTNPP. However, ‘Araarsaa’, ‘Dagim’, and ‘Zengena’ produced the lowest mean value of this parameter (4.0). On the other hand, ‘Gudenie’ registered the highest mean value (4.6) of UMTNPP, but ‘Chiro’ and ‘Bedassa’ produced the lowest value (2.0) of this parameter. ‘Bubu’ produced the highest mean value (62.3 g) of the ATW, but ‘Gera’ had the lowest (45.0 g). ‘Gudenie’ recorded the highest mean value (25.5 t ha−1) of MY. However, ‘Araarsaa’, ‘Dagim’, and ‘Zengena’ produced the lowest mean values for this parameter. ‘Gudenie’ produced the highest TY (39.2 t ha−1), but ‘Araarsaa’, ‘Bedassa’, ‘Dagim’, and ‘Zengena’ produced the statistically lowest mean values (17.5–19.6 t ha−1) of TY.
At ‘Arbarakate’, ‘Chiro’ generated the highest mean value (68.3 g) of ATW, while ‘Gera’ recorded the lowest value (40.6 g). ‘Chiro’, ‘Gudenie’, ‘Jalenie’, and ‘Bubu’ produced higher mean values that ranged from 33.5 to 37.5 for MY t ha−1, but ‘Gera’, ‘Araarsaa’, ‘Bedassa’, ‘Dagim’, and ‘Zengena’ produced lower values (18.6–22.5 t ha−1) for this parameter. At this location, the highest mean value (49.6 t ha−1) of TY was registered in ‘Bubu’, however ‘Gera’, ‘Bedassa’, and ‘Dagim’ produced lower values of the parameter (Table 4).
Genotypic and Phenotypic Correlations
Genotypic correlation coefficients were computed for MI, RS, and plant-related parameters. The results revealed a positive and highly significant genetic correlation between G/R and BWI (rg = 0.74). However, G/R had negative and highly significant correlations (rg = − 0.43) with UMY. G/R also had a negative but significant genetic correlation with the other plant parameters studied. RGI had a positive and significant genetic correlation (rg = 0.53) with BWI. ATW, MY, and UMY had positive and significant genetic correlations with TY.
A positive and highly significant phenotypic correlation (rp = 0.74) was observed between G/R and BWI. MTNPP showed a negative but highly significant phenotypic correlation with G/R and RGI. UMTNPP had a negative and highly significant phenotypic correlation with MTNPP (rp = − 0.83). MY showed a positive and highly significant phenotypic correlation with ATW (rp = 0.91) but a negative and highly significant correlation with UMY. Generally, TY had a negative phenotypic correlation with the pathogen parameters, but this was positive with all plant-related parameters studied (Table 5).
Genetic Distance
‘Gera’ was the most distant from all tested cultivars with ED = 30.87. ‘Gudenie’ was found to be distant from most of the cultivars except from ‘Belete’ (ED = 7.62) and ‘Bubu’ (ED = 7.48). ‘Jalenie’ was found to be distant from most of the tested cultivars except from ‘Shonkolla’ (ED = 6.48). ‘Dagim’ was the closest to ‘Zengena’ with ED = 2.45 (Table 6).
Clustering of Potato Cultivars
The tested potato cultivars were clustered into five based on Euclidean distance. Cluster I consisted of three cultivars (‘Dagim’, ‘Zengena’, and ‘Bedassa’) characterised by low yield. Cluster II consisted of two cultivars (‘Araarsaa’ and ‘Gera’), cluster III (‘Chiro’, ‘Jalenie’, and ‘Shonkolla’), and cluster IV (‘Mara Charre’ and ‘Guassa’). The last (cluster V) showed resistance to MI and RS and had a high yield consisting of ‘Belete’, ‘Bubu’, and ‘Gudenie’ (Fig. 1).
Clusters III and V registered the highest and lowest mean values (33.5 and 17.2, respectively) of the number of galls per root system and tuber. These clusters also registered the highest and lowest mean values (0.67 and 0.31, respectively) of bacterial wilt index. Clusters I and V recorded the lowest and highest mean values (10.8 and 24.1 t ha−1), respectively, of marketable tuber yield. The clusters also registered the lowest and highest mean values (6.6 and 12.3 t ha−1) of unmarketable tuber yield and 17.1 and 36.5 t ha−1 of total tuber yield, respectively (Table 7).
Heritability of the Parameters
The heritability (H2b) in a broad sense for all parameters studied was computed and categorised as suggested by Johnson et al. (1955). Accordingly, the RGI and ATW fell under the medium category, while the rest of the parameters fell under high in ‘Kersa’. All the parameters from ‘Arbarakate’ fell under the high H2b category (Table 8).
Discussion
The mean square values from the analyses of variance for MI, RS, and plant-related parameters of potato cultivars at ‘Kersa’ and ‘Arbarakate’ showed highly significant differences among all parameters, indicating the influence of environment on the parameters and the presence of genetic variability in the tested potato cultivars. UMTNPP, ATW, MY, and TY were significantly influenced by cultivar and cultivars × location interaction, evidencing the various responses of the cultivars across locations. Significant effects of cultivars, location, and their interaction on the yield parameters of potato cultivars have been reported in Ethiopia (Berhanu and Tewodros 2016; Wassu 2016; Tessema et al. 2020). The presence of significant differences among released potato cultivars for MY and TY has been reported in Ethiopia (Habtamu et al. 2016; Tessema et al. 2020). Wassu (2016) also reported the presence of significant differences among 16 released potato cultivars for MY, TY, and late blight disease resistance in Ethiopia.
At ‘Kersa’, ‘Gudenie’ and ‘Guassa’ produced the highest MTNPP. ‘Bubu’ produced the highest ATW. At ‘Arbarakate’, in most cultivars, higher MTNPP was recorded. ‘Chiro’ generated the highest ATW. Previously, significant differences among potato cultivars and their growing environments for MTNPP and ATW were reported by Berhanu and Tewodros (2016), Habtamu et al. (2016), Seifu and Betewulign (2017), and Tessema et al. (2020). Eaton et al. (2017) reported that genetic variation among cultivars, management practice, or agroecological conditions could contribute to the variations in performance among potato cultivars.
Significant differences among potato cultivars in their reaction to RKN have been reported, with the ‘highly resistant’ cultivars containing fewer developed nematodes than ‘susceptible’ cultivars (Bekhiet et al. 2010; Hussain et al. 2016; Montasser et al. 2019; Getu et al. 2021). At ‘Kersa’, ‘Gudenie’, ‘Mara Charre’, ‘Belete’, and ‘Bubu’ showed light galling, while comparisons with the results from the other cultivars showed that the tested potato cultivars had genetic variability for this trait. At this location, none of the tested cultivars was resistant to MI.
Heritability estimates provide information about the likelihood that a particular genetic attribute will be transmitted to the successive generation (Marwede et al. 2004). The H2b for all parameters studied was computed. Accordingly, at ‘Kersa’, ATW falls under medium, while the other parameters are grouped under high. All parameters from ‘Arbarakate’ grouped under high H2b, suggesting that the parameters tested could be further improved through selection. Plant parameters that have high H2b are likely to be favourable targets in plant breeding programmes for developing better potato cultivars.
When two parameters are highly genetically correlated, the genes that contribute to the parameters are usually co-inherited (Lynch and Walsh 1998). In the current study, genotypic and phenotypic correlation coefficients were computed for MI, RS, and plant-related parameters. The coexistence of the two pathogens has been reported by many workers on various hosts (Bekhiet et al. 2010; Ghosh et al. 2016; Sundaresh et al. 2017). G/R and BWI had negative and highly significant correlations with unmarketable tuber yield. This suggests that MI and RS disease complex is linked to unmarketable tuber yield but adversely impact marketable tuber yield.
The Euclidean distance is a measure of dissimilarity in both morphological and molecular analyses. Dissimilarity coefficients estimate the distance or unlikeness of two individuals; the larger the value, the more different are the two individuals (Persson 2001). Populations with many similar genes have small genetic distances (Osawaru et al. 2015). In the present work, ‘Gera’, which fell under ‘moderately resistant’ and ‘susceptible’ to MI and RS, respectively, was the most distant from all tested cultivars. This implies this cultivar was the most different to the other tested potato cultivars. ‘Gudenie’ was found to be close to ‘Belete’ and ‘Bubu’, suggesting that they are closely related and/or have a recent common ancestor.
In a cluster analysis, relatively homogeneous groups of individuals cluster together hierarchically, and this clustering is displayed in a dendrogram (Holland 2006; Osawaru et al. 2015). In this study, ‘Dagim’, ‘Zengena’, and ‘Bedassa’, which were characterised by low yield, have been clustered together, whereas ‘Belete’, ‘Bubu’, and ‘Gudenie’ resistant to MI, RS, and which showed higher yields, were clustered together. This suggests the cultivars within a cluster were homogeneous.
Conclusions
From the present study, it can be concluded that all potato cultivars assessed performed differently in terms of their reactions to Meloidogyne incognita, Ralstonia solanacearum, and yield-related parameters. A significant interaction between the cultivars and locations was seen. Positive and highly significant genotypic and phenotypic correlations were recorded between MI and RS. The pathogens had negative and highly significant correlations with the studied plant-related parameters. At ‘Kersa’, ‘Gudenie’ recorded the highest mean value (25.5 t ha−1) of marketable yield. At ‘Arbarakate’, ‘Chiro’, ‘Gudenie’, ‘Jalenie’, and ‘Bubu’ produced higher mean values that ranged from 33.5 to 37.5 t ha−1 for this parameter. These data provide information for breeders for further improvement through selection since most of the studied parameters were shown to be heritable.
Data Availability
Raw data were generated at Haramaya University. Derived data supporting the findings of this study are available from the corresponding author [Tasew Getu] on demand.
Code Availability
Not applicable.
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Acknowledgements
This work is part of a Ph.D. dissertation of the first author. The authors are thankful to the Ministry of Science and Higher Education (MoSHE) and Haramaya University (HU) for their extended material, technical, and financial support in carrying out this study.
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The research was funded by the Ministry of Science and Higher Education and Haramaya University, Ethiopia.
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All authors contributed to this part of the Ph.D. dissertation of the first author. Material preparation, data collection, and analysis were performed by Tasew Getu. The first draft of the manuscript was written by Tasew Getu, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Getu, T., Mohammed, W., Seid, A. et al. Evaluation of the Reaction of Various Potato (Solanum tuberosum L.) Cultivars to the Meloidogyne incognita and Ralstonia solanacearum Disease Complex under Field Conditions. Potato Res. 67, 73–91 (2024). https://doi.org/10.1007/s11540-023-09624-w
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DOI: https://doi.org/10.1007/s11540-023-09624-w