Abstract
The global HIV/AIDS pandemic has claimed the lives of an estimated 35 million people. A significant barrier for combating this global pandemic is substance use since it is associated with HIV transmission, delayed diagnosis/initiation of therapy, and poor adherence to therapy. Clinical studies also suggest a link between substance use and HIV-disease progression/AIDS-associated mortality. Methamphetamine (METH) use is one of the fastest-growing substance use problems in the world. METH use enhances high-risk sexual behaviors, therefore increases the likelihood of HIV-1 acquisition. METH use is also associated with higher viral loads, immune dysfunction, and antiretroviral resistance. Moreover, METH use has also been correlated with rapid progression to AIDS. However, direct effects of METH on HIV-1 disease progression remains poorly understood because use of METH and other illicit drugs is often associated with reduced/non adherence to ART. Nevertheless, in vitro studies demonstrate that METH increases HIV-1 replication in cell cultures and animal models. Thus, it has been proposed that METH’s potentiating effects on HIV-1 replication may in part contribute to the worsening of HIV-1 pathogenesis. However, our recent data demonstrate that METH at physiologically relevant concentrations has no effect and at higher concentrations inhibits HIV-1 replication in CD4+ T cells. Thus, the goal of this review is to systematically examine the published literature to better understand the complex interaction between METH abuse and HIV-1 disease progression.
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Introduction
The HIV/AIDS pandemic has caused approximately 35 million deaths worldwide1. In addition, an estimated 34 million people are living with the virus worldwide (http www who int mediacentre factsheets fs360 en index html 2999). In the US there are ~1.2 million people who are infected with HIV (CDC 2012). Even though HIV research has witnessed tremendous progress in last three decades a preventative vaccine has eluded the scientific community. Therefore, antiretroviral therapy (ART) continues to be the only treatment option available to HIV infected patients (Walensky et al. 2006). The effectiveness of ART has dramatically reduced HIV/AIDS related mortality (Walensky et al. 2006). Unfortunately, only ~25 % of the HIV-1 infected patients in the US have their virus under control (CDC 2012). Thus majority of HIV-1 infected people in the US are either unaware of their infection status or not connected/retained to care or are non-adherent to ART (CDC 2012). The problem of this ongoing worldwide pandemic is further accentuated by substance use that serves as a powerful cofactor at every stage of HIV/AIDS disease including transmission, diagnosis, pathogenesis, and treatment (Kipp et al. 2011; Friedman et al. 2006). Clinical studies suggest that substance use may increase viral load, accelerate disease progression and worsen AIDS-related mortality even among ART adherent patients (Kipp et al. 2011; Friedman et al. 2006). However, establishing a direct link between substance use and HIV/AIDS in human patients remains highly challenging. This is in part because drug use is often associated with reduced/non adherence to ART that severely complicates a direct correlation between substance use and worsening of HIV-1 disease (Qian et al. 2011; Parsons et al. 2013; Marquez et al. 2009). In addition, there are also confounding factors associated with studying drug abusing patients that can influence outcomes of HIV disease. These include history and route of drug use, amount and formulation of drug used, single or concurrent use of other drugs, and poor nutrition (Qian et al. 2011; Parsons et al. 2013; Marquez et al. 2009). These multifaceted problems have posed serious obstacles to researchers for delineating the mechanisms by which substance abuse exacerbates HIV disease. Nevertheless, it is becoming clear that illicit drugs such as METH, cocaine, opiates, marijuana, alcohol and others regulate HIV-1 infection/replication in vitro and in animal models (Wang and Ho 2011). Moreover, published data is shedding light on the negative effects of substance use on immune system that controls viral infections (Wang and Ho 2011). These studies are paving the way for better understanding the comorbid condition of drug use and HIV/AIDS.
METH is one of the fastest-growing substance use problems in the world and continues to rise to epidemic conditions (Misawa et al. 2011). METH use has been linked to worsening of HIV-1 disease and AIDS-related outcomes (Parsons et al. 2013; Ellis et al. 2003; Carrico et al. 2007; Fairbairn et al. 2011; King et al. 2009). Moreover, in vitro and animal studies demonstrate that METH enhances HIV-1 infection/replication (Wang and Ho 2011). We have recently reported that METH at physiological concentrations has limited impact on HIV-1 replication in CD4+ T cells (Mantri et al. 2014) that serves as the primary targets of HIV-1 in vivo (Lane 2010). Therefore, in this review we will highlight the interplay between METH abuse and HIV/AIDS and discuss how this interaction may contribute to HIV disease (Fig. 1). Specifically, we will describe the prevalence and impact of METH abuse on; HIV-1 transmission, replication, viral load, alteration in immune function, adherence to ART, and AIDS related outcomes. We will also summarize the potential mechanisms by which METH regulates HIV-1 replication. Finally, we will discuss the unanswered questions on the synergy between METH and HIV/AIDS as part of the future prospective. The impact of METH abuse on neurological manifestations of HIV-1 infected and un-infected patients has been extensively reviewed in recent years (Gaskill et al. 2013; Cisneros and Ghorpade 2012; Nair and Samikkannu 2012), therefore will not be covered in this review.
Prevalence of METH Use and Increased Risk of HIV Transmission
METH is the second most popular illicit drug used in the world with an annual prevalence estimated at 35 million or 0.4 % of the global population (Misawa et al. 2011). This unprecedented number of METH users exceeds that for cocaine and heroin (Misawa et al. 2011). Moreover, use of this drug is especially common amongst young individuals in US and Canada (Gruenewald et al. 2010). For example, in the US METH use has reached epidemic levels with an estimated 1.5 million regular users and ~11 million reported to use it at least once in their lifetime (Colfax and Shoptaw 2005; Rawson et al. 2002). This high prevalence has been attributed to wide availability, low cost, long duration of psychoactive effects, and the ease of use of METH by smoking, snorting, chewing, swallowing or injecting (Misawa et al. 2011; Gruenewald et al. 2010; Colfax and Shoptaw 2005; Rawson et al. 2002). Since METH use has serious social, economic, biological, and health effects, this ever increasing number of METH users underscore the serious drug abuse challenge in the US and the world.
METH is known to induce the release of neurotransmitters that produce sensations of pleasure and enhance at-risk sexual behavior (Semple et al. 2002). Therefore, use of METH has been linked to increased likelihood of acquiring sexually transmitted infections including that of HIV (Rawson et al. 2002; Semple et al. 2002; Hurt et al. 2010; Patterson et al. 2008; Wohl et al. 2002; Peck et al. 2005). For example, METH is commonly used among men who have sex with men (MSM) compared to the general population. In a study conducted by Thied et al. in 7 US urban areas, 20 % of young MSM (aged 15–22 years) reported METH use during the past 6 months (Thiede et al. 2003). In addition, Mansergh et al. reported an estimated 15 % of MSM in San Francisco had used METH within the past 3 months (Mansergh et al. 2006). Additionally, in a multicenter prospective cohort study of MSM, Plankey et al. found that METH users were more likely than non-users to acquire HIV (Plankey et al. 2007). Therefore, METH use has been associated with doubling the risk of HIV-1 acquisition among MSM (Plankey et al. 2007; Halkitis et al. 2001; Drumright et al. 2006; Shoptaw and Reback 2006). Furthermore, METH is also used widely as a recreational drug among heterosexual men (Wohl et al. 2002) and female sex workers (Patterson et al. 2008). The association between METH use and HIV-1 transmission is demonstrated in a reasonably wide demographic of people by Patterson et al. (Patterson et al. 2008). These authors reported that smoking, snorting, or inhalation of METH was independently associated with HIV infection (Patterson et al. 2008). A 2002 case–control study by Wohl et al. enrolling African-American heterosexual men showed that METH use was positively associated with HIV infection (Wohl et al. 2002). These studies strongly suggest a link between METH use and HIV-1 infection. Even though this interaction is heightened in MSM it extends across multiple populations, including female sex workers, and heterosexual men. These studies also propose that the increased transmission is driven by METH use associated high-risk sexual practices, such as sex with anonymous and multiple partners, marathon sex, and unprotected sex. Most importantly, the high prevalence of METH use among HIV-1 infected and at-risk population underscore the critical need to examine the impact of METH use on every aspect of HIV-1 disease.
METH Use is Associated with Worsening of HIV Disease Progression
Epidemiological studies suggest that substance use including that of METH is associated with accelerated disease progression (Parsons et al. 2013; Ellis et al. 2003; Carrico et al. 2007; Fairbairn et al. 2011; King et al. 2009; Shoptaw et al. 2012). This could be attributed to several mechanisms including increased viral load, accelerated CD4+ T cell decline, immune modulation, and reduced/non adherence to ART (Fig. 1). Published studies described below and summarized in Table 1 provide evidence that METH use may affect many of these factors individually or in combination to worsen HIV-1 disease progression.
Given that METH use is prevalent among HIV-1 infected people; several studies have evaluated the effects of METH use on HIV-1 viral load in these patients. In a 2003 study, Ellis et al. reported that among a subgroup of patients receiving ART, METH use was significantly correlated to increased plasma virus load (Ellis et al. 2003). This was further supported by Carrico et al. describing that among ART adherent patients, regular stimulant users including that of METH had a five-fold higher viral load than the nonusers (Carrico et al. 2007). Similarly, Fairbairn et al. illustrated a negative association between METH use and viral load suppression (Fairbairn et al. 2011). Notably, a study by King et al. reported that regardless of ART adherence participants reporting drug use in the past 30 days were significantly more likely to have detectable viral loads (King et al. 2009). The association between METH use and viral load in this study was about 45 % stronger than use of other drugs (King et al. 2009). This study highlights the unique nature of METH abuse compared to other illicit drugs such as cocaine/crack, heroine, and other drugs. Collectively, these studies indicate an association between METH use and elevated viral load that could contribute to accelerated disease progression.
Stimulant use including that of METH is associated with impaired adherence to ART among HIV-positive individuals. A 2013 study by Parsons et al., demonstrated that METH use resulted in a significant reduction in ART adherence (Parsons et al. 2013). Hinkin et al. reported similar results showing that drug users including that of METH were four times less likely to adhere to ART (Hinkin et al. 2007). In another study, Carrico et al. also reported a negative association between METH use and ART adherence among HIV-positive METH users (Carrico et al. 2010). These studies demonstrate that, reduced adherence to ART among METH users may play a critical role in increasing viral load and worsening of HIV-1 disease.
Decline in CD4+ T cell counts and an increase in CD8+ T cell counts serve as an important diagnostic for HIV-1 disease progression. A study by Shoptaw et al. examined the association between stimulant use including that of METH with immune function biomarkers among both HIV-positive and HIV-negative men (Shoptaw et al. 2012). Data from this study noted significant negative associations between METH use and CD4+/CD8+ ratios for HIV-positive men (Shoptaw et al. 2012). Notably, these authors also described that METH use decreased CD4+/CD8+ cell count ratio for those HIV-infected men on an ART regimen (Shoptaw et al. 2012). This study indicated that METH use can contribute to HIV-1 disease progression even among ART adherent patients.
In addition to affecting viral load, ART adherence and CD4+ T cell numbers, METH use has been suggested to exacerbate HIV-1 disease by other mechanisms. For example, Cachay et al. reported that in newly diagnosed, ART naïve individuals, those who reported using METH within the 30 days were more likely to have transmission of drug resistant HIV-1 (Cachay et al. 2007). In another case study, Markowitz et al. reported an association between METH use with infection of a multidrug resistant, dual-tropic HIV-1 that resulted in rapid progression to AIDS (Markowitz et al. 2005). Furthermore, Colfax et al. demonstrated that in a multivariate model controlling for multiple sex partners, race/ethnicity, and other illicit drug use, frequent METH use associated with HIV-1 drug resistance (Colfax et al. 2005).
Collectively, these studies describe an association between METH use and increased viral load/decreased CD4+ T cell counts and other mechanisms that have the potential to worsen HIV-1 disease progression. However, a direct link between METH use and HIV disease in human patients remains contentious. This is because use of METH and other illicit drugs is often associated with reduced/non adherence to ART (Qian et al. 2011; Parsons et al. 2013; Marquez et al. 2009; Arnsten et al. 2002; Reback et al. 2003). In addition, history and route of drug use, amount and formulation of drug used, single or concurrent use of other drugs, and poor nutrition confounds establishing a functional correlation between drug use and HIV-1 disease (Qian et al. 2011; Parsons et al. 2013; Marquez et al. 2009; Arnsten et al. 2002; Reback et al. 2003). Therefore, further research in well-defined cohorts is warranted to examine the direct impact of METH use on HIV-1 disease progression.
METH Treatment Enhances Viral Load in Animal Models
To address some of the caveats associated with clinical studies described in the previous section, Marcondes et al. examined effects of chronic METH treatment on pathogenesis of Simian Immunodeficiency Virus (SIV) in a rhesus macaque model (Marcondes et al. 2010). The infection of rhesus macaques with SIV results in disease conditions that mimic that of HIV-1 disease in humans including progressive loss or dysfunction of the immune system resulting in immune deficiency (Marcondes et al. 2010). Therefore, this study investigated effects of METH on viral load as well as differences in immune cells in chronically infected rhesus macaques. These authors reported that METH administration did not alter levels of virus in the plasma. Intriguingly, data in this report demonstrated that viral load in the brain was significantly increased in METH-treated animals compared with control animals. Moreover, these authors also provided evidence that viral infection resulted in a reduction in CD4+ T cells in METH treated animals. Given that this study mimicked the amounts of drug used by chronic METH abusers, the authors emphasized that METH abuse may directly impact AIDS progression by reducing CD4+ T cells even though METH’s effect on peripheral viral load was negligible.
In another study, Toussi et al. evaluated the direct effects of METH on HIV-1 replication using the JR-CSF/hu-CycT1 HIV transgenic mouse model (Toussi et al. 2009). This transgenic mouse model has an integrated copy of R5 tropic HIV-1 provirus that expresses HIV-1 proteins and develops plasma viremia. Specifically these mice express human cyclin T1 gene enabling support of Tat-mediated HIV-1 production in the mouse CD4+ T cells and myeloid-lineage cells (Toussi et al. 2009). Using this model, Toussi et al. reported that in comparison to splenocytes from control mice, the HIV-1 p24 antigen content in the splenocytes of METH-treated mice was increased significantly (Toussi et al. 2009). They also measured the impact of METH exposure on plasma HIV-1 RNA levels in these mice. Data in this report demonstrated that in contrast to control mice, administration of METH significantly increased HIV-1 viremia by almost 6-fold. However, these authors highlighted that these transgenic mice are populated with mouse CD4-expressing T cells and macrophages that cannot be infected with HIV-1 (Toussi et al. 2009). Therefore, the increased HIV-1 production and viremia was hypothesized to be dependent on METH’s effect on the post-integration stages of HIV-1 replication6. Nevertheless, these data provided some insights into the mechanisms by which METH can accelerate HIV-1 disease.
These two animal studies support the potentiating effects of METH on HIV-1 infection and provide some mechanistic insights into the effects of METH abuse on HIV-1 pathogenesis. However, there are no follow up studies that support the effects of METH on HIV-1 infection, replication/viral load and disease progression. Therefore, additional studies are needed to clearly demonstrate the potentiating effects of METH on HIV-1 pathogenesis.
METH Modulates HIV-1 Infection/Replication in the Cultures of Permissive Cells
CD4+ T cells serve as the primary targets of HIV-1 infection and replication in vivo even though other permissive cells such as monocytes, macrophages, dendritic cells and to some extent astrocytes support HIV-1 infection/replication. in vitro data provide strong evidence that METH potentiates HIV-1 infection/replication in these permissive cells (Nair et al. 2009), (Liang et al. 2008). Nair et al. have reported that METH at concentrations 10–100 μM increased infection of X4 tropic HIV-1 virions in monocyte derived dendritic cells (Nair et al. 2009). These authors proposed that METH enhanced HIV-1 infection in these cells by upregulating the HIV-1 co-receptors, CXCR4 and CCR5 (Nair et al. 2009). In addition, data in this report suggested that METH mediated increase in HIV-1 infection is dependent on the downregulation of extracellular-regulated kinase (ERK2) and the upregulation of p38 mitogen-activated protein kinase (MAPK) (Nair et al. 2009). Notably, dendritic cells are susceptible to infection by both R5 and X4 tropic HIV-1 virions since they express the HIV receptors and co-receptors such as CD4, CCR5, CXCR4, and others (Nair et al. 2009). Moreover, R5 HIV-1 virions are known to infect dendritic cells more efficiently than X4 tropic virions (Nair et al. 2009). Therefore, elucidating the effects of METH on R5 tropic virions in dendritic cells is warranted to gain better insights into the potentiating effects of METH on HIV-1 infection and replication.
Liang et al. reported that METH at concentrations 1–250 μM enhanced HIV infection of monocyte derived macrophages (MDMs) (Liang et al. 2008). These authors demonstrated that METH treatment resulted in a significant and dose-dependent increase of reverse transcriptase activity in MDMs infected with R5 tropic HIV-1 virions. They also illustrated that METH’s potentiating effects on HIV-1 was dependent on dopamine D1 receptor and the entry co-receptor CCR5 (Liang et al. 2008). Additionally, data in this report demonstrated that METH inhibited interferon-α (INF- α) and signal transducer and activator of transcription-1 (STAT1) (Liang et al. 2008). Similarly, another study by Gavrilin et al. demonstrated that METH increases infection/replication of feline immunodeficiency virus (FIV) in feline astrocytes (Gavrilin et al. 2002). These authors proposed that the effect of METH is dependent on the viral entry or integration step, but not at the translational level (Gavrilin et al. 2002). Given that astrocytes to some extent support HIV-1 in the central nervous system (CNS), this data corroborated the findings of Marcondes et al. (Marcondes et al. 2010) that METH enhances viral replication in the brain.
Even though CD4+ T cells are the primary targets of HIV-1 in vivo, studies examining the effects of METH on HIV-1 replication in human CD4+ T cells are limited. Recently, we reported the impact of METH treatment on HIV-1 replication in primary activated CD4+ T cells isolated from the PBMCs of healthy donors (Mantri et al. 2014). Our data revealed that METH at concentrations 1–50 μM had no effect on HIV-1 replication in CD4+ T cells (Mantri et al. 2014). This observation corroborated the findings of Marcondes et al. that METH administration did not alter levels of virus in the plasma of SIV infected rhesus macaques (Marcondes et al. 2010). Surprisingly, our studies illustrated that at concentrations 100–1000 μM, METH treatment inhibited viral replication in CD4+ T cells in a dose dependent manner. We used these concentrations to mimic that of METH abusers that varies between 10 and 50 μM in blood and 240–1000 μM in spleen and brain (Mantri et al. 2014). The inhibitory effects of METH at 100–1000 μM is contrary to the study by Toussi et al. that described METH at concentrations 10–150 μM enhances HIV-1 replication in the CD4+ T cells of HIV-1 transgenic mouse (Toussi et al. 2009). It is important to point out that this double transgenic mice has an integrated HIV-1 provirus in every cell type (Sun et al. 2006). Therefore, the systemic effects of METH on viral gene expression from every mouse cells cannot be differentiated from the direct effects of METH on HIV-1 in CD4+ T cells. Given that this mouse model is not a humanized mouse model, the mouse cells in this model cannot be infected with HIV-1. Therefore, data reported by Toussi et al. only examines effects of METH on HIV-1 LTR-driven transcription but not infection or replication of the virus. Furthermore, the integrated provirus in this animal model is a R5 tropic virion in contrast to the X4 tropic virions used in our studies. We must point out that our data was derived by infecting of purified CD4+ T cells but not in the mixed culture of PBMCs. Whether similar effects of METH on HIV could be obtained with PBMCs are currently underway.
Toussi et al. also reported that METH at 10–150 μM also increased HIV-1 replication in primary activated CD4+ T cells (Toussi et al. 2009). However, these authors used R5 tropic HIV-1 virions to infect human CD4+ T cells in contrast to X4 tropic HIV-1 virions used in our study. It is well established that X4 virions utilize the CXCR4 co-receptor whereas R5 tropic virions utilize CCR5 co-receptor for cellular entry. Since primary CD4+ T cells express both CXCR4 and CCR5 co-receptors these cells can be infected by both X4 and R5 virions. Surprisingly, our studies also showed inhibitory effects of METH on replication of R5 HIV-1 virions in primary CD4+ T cells (Mantri et al. 2014). One major difference in our infection studies with R5 virions is that we measured intracellular p24 in contrast to extracellular p24 measurements by Toussi et al. We speculate that the methods used cannot explain the contrasting data since extracellular p24 levels is dependent on intracellular p24 production. Furthermore, when the co-receptor requirement for viral entry was abrogated by pseudotyping (Mantri et al. 2014), METH also inhibited replication of HIV-1 virions in CD4+ T cells. These data strongly suggest that METH’s effect on HIV-1 replication in CD4+ T cells may not depend on co-receptor requirement. A major difference is that Toussi et al. added METH to the cells every day, whereas in our study cells were treated with METH only once. Whether, these distinct pattern of METH exposure is responsible for the contrasting effects of METH on HIV-1 replication is currently under investigation. Given that these studies used primary cells from human donors, the contribution of genetic variability among donors cannot be ruled out. The genetic variability among donors may affect the expression of cellular factors that modulate HIV-1 infection and replication in the CD4+ T cells. Nevertheless, these discrepancies in METH’s effect on HIV-1 replication highlight the critical need for comprehensive research to better understand the complex interaction between METH and HIV-1 in CD4+ T cells and other permissive cells.
METH Regulates Both Entry and Post Entry Steps of HIV-1 Infection
Published data provide strong evidence that METH potentiates HIV-1 replication by modulating the entry of virions into target cells. For example, Nair et al. proposed that METH enhanced HIV-1 entry into dendritic cells by upregulating the co-receptors, CXCR4 and CCR5 (Nair et al. 2009). In another study, Nair et al. suggested that METH modulates HIV-1 entry into dendritic cells by regulating the receptor DC-SIGN (Nair et al. 2006). In addition, Liang et al. has illustrated that METH can increase HIV-1 entry into in MDMs by regulating expression of CXCR4 and CCR5 co-receptors45. METH also has been shown to reduce secretion of the chemokines MIP-1α, MIP-1β, and RANTES which can bind to the CCR5 co-receptor and prevent HIV-1 entry into the target cells (Nair and Saiyed 2011). Moreover, our data on VSV-G pseudotyped HIV-1 virions suggested that METH may also target viral post entry steps to regulate HIV-1 replication (Mantri et al. 2014). HIV-1 post entry steps include reverse transcription, integration, transcription, translation and assembly. There is evidence that METH regulates HIV-1 LTR driven transcription in microglial cells (Wires et al. 2012). We have demonstrated that METH regulates HIV-1 protein translation in CD4 + T cells (Mantri et al. 2014). Interestingly, our data also revealed that METH achieves this by targeting the cellular anti-HIV-1 miRNAs that are known to negatively regulate HIV-1 protein translation (Mantri et al. 2014). Given that HIV-1 post entry steps are dependent on a complex interaction between the virus and cellular host factors, METH can influence HIV-1 post entry steps by targeting HIV-1 associated cellular factors (Nair et al. 2009; Liang et al. 2008). In sum, these published data suggest that METH can modulate HIV-1 infection/replication via direct and indirect mechanisms. However, the molecular details and the underlying mechanisms by which METH regulates HIV-1 entry and post-entry steps are yet to be clearly understood.
Immunomodulatory Functions of METH May Modulate HIV-1 Infection and Replication
In addition to directly modulating various entry and post entry steps of HIV-1 infection, the immunomodulatory effects of METH may also contribute towards its effects on HIV-1 life cycle. METH is known to stimulate release of dopamine, serotonin, and norepinephrine (Semple et al. 2002; Reiner et al. 2009). The receptors and transporters that mediate effects of these neurotransmitters are also expressed on various immune cells. Therefore, METH use has been shown to alter or suppress functions of immune cells that are implicated in HIV-1 pathogenesis. For example, METH is known to regulate T cell function including proliferation, cytokine production and T cell-mediated immune response (Potula and Persidsky 2008; Harms et al. 2012). In addition, METH is demonstrated to downregulate the expression of anti-viral cytokine IFN-α in macrophages and dendritic cells (Liang et al. 2008; Mahajan et al. 2006). Moreover, Gavrilin et al. have shown that METH suppresses interlukin-2 (IL-2) and IFN-γ expression (Gavrilin et al. 2002). There is also evidence that METH stimulates secretion of Tumor Necrosis Factor-alpha (TNF-α) in the splenocytes of retrovirus-infected mice (Yu et al. 2002). METH’s immunomodulatory effect is further supported by Yu et al. demonstrating that METH exposure inhibited macrophage-mediated antiviral activities by reducing production of nitric oxide (NO)/TNF- α (In et al. 2004). In addition, METH has been shown to reduce the expression of the dendritic cell marker CD83 that plays an important role in antigen presentation and T cell activation (Nair et al. 2006). Microarray analysis of brain tissue from HIV-infected METH users by Everall et al. showed significant up-regulation of inflammatory genes (Everall et al. 2005). In another microarray study on dendritic cells, Mahajan et al. have illustrated that METH alters expression of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-8 (Mahajan et al. 2006). Talloczy et al. have also demonstrated that METH alters the function of the murine immune system including that of dendritic cells and macrophages, and T-cell antigen presentation (Talloczy et al. 2008). In addition, these authors reported that use of METH increases cytokines, chemokines, and cellular adhesion molecules in murine models and human participants (Loftis et al. 2011). Furthermore, data from chronically SIV-infected rhesus macaques showed that METH treatment induces activation of natural killer cells (Marcondes et al. 2010). Taken together, these studies provide strong evidence that the immunomodulatory functions of METH could contribute to HIV infection and exacerbate disease progression.
Recent studies indicate that METH’s immune suppressive function may also depend on its ability to alkalize acidic organelles within immune cells. METH’s ability to alkalize acidic organelles may inhibit antigen presentation, impairs phagocytosis, and likely compromises the immune response to pathogen inactivation (Martinez et al. 2009; Newman 1999; Chaturvedi et al. 1995). For example, it has been showed that METH inhibits endosomal acidification in macrophages (Martinez et al. 2009; Newman 1999; Chaturvedi et al. 1995). A neutral pH of endosomes has been shown to have beneficial effects for pathogens during infection of macrophages (Eissenberg et al. 1993). In addition, METH has also been reported to destabilize the capacity of infected macrophages to regulate their intracellular milieu (Martinez et al. 2009; Newman 1999; Chaturvedi et al. 1995). In macrophages endosomal trafficking plays critical roles in HIV-1 replication (Pelchen-Matthews et al. 2003). Therefore, effects of METH on endosomal pathways may directly and indirectly contribute to HIV-1 infection. In addition, acidification of organelles can potentially have devastating effects in HIV‐infected METH abusers with reference to opportunistic infections. Whether the effects of METH on endosomal pathway may contribute to the discrepant data reported in the literature is yet to be determined.
Concluding Remarks and Future Prospective
METH is a highly addictive psychostimulant that modulates release of neurotransmitters in the brain and causes euphoria. Therefore, METH use and HIV-1 infection frequently coexist because of the association of this drug with engagement of high-risk behaviors. Moreover, clinical studies suggest that METH users may display higher levels of HIV loads than nonusers. Even though the biological effects of METH in the CNS have been extensively studied, the interaction between METH use and HIV/AIDS remains poorly understood. Published studies from cell culture models and animals implicate increased viral replication for the potential effects of METH on HIV-1 pathogenesis. However, the direct effects of METH use on HIV infection and HIV disease progression are still poorly understood and remain contentious. In particular, the deleterious effect of METH on the host’s immune response and its role in the immunopathogenesis of HIV remain to be elucidated. Even though a plethora of data are available on the biochemical and molecular effects of METH on an array of cell types, there are few studies that examine molecular effects of METH on immune cells specifically on T cells. Most importantly, the mechanisms by which METH regulates HIV-1 infection and replication in various permissive cells including CD4+ T cells in the periphery remain largely unclear. The contrasting data on the potentiating and inhibitory effects of METH on HIV-1 replication in vitro underscore the need to investigate the interaction between METH and HIV-1 at molecular level. The published data that METH has no/limited impact on peripheral viral load in SIV infected animals add to the biological and molecular intricacy of this drug. Given that there are only two animal studies that examine METH’s effect on HIV-1 infection and replication, whether the reported differential effects of METH on HIV-1 is dependent on the type of cells infected and/or the tropism of virions should be further investigated. Most importantly, whether the contrasting effect of METH on HIV-1 is dependent on local concentrations of METH in the tissues is yet to be determined.
In summary, clinical studies suggest an association between METH abuse and HIV-1 pathogenesis. One animal study using transgenic mice indicate that METH potentiates HIV-1 replication, whereas the SIV study suggest that METH has minimal or no effects on the viral load in the periphery but enhances viral load in the brain. There is evidence that METH enhances HIV-1 infection in the cultures of astrocytes, dendritic cells and macrophages. Our data illustrate that METH’s effect on HIV-1 in CD4+ T cells may depend on concentrations used and to some extent tropism of the HIV-1 virions. Collectively, these clinical, animal-based and in vitro studies highlight that METH’s effect on HIV-1 pathogenesis is complex and comprehensive studies are warranted for unequivocal data. Given that humanized mouse models are extensively used to study HIV-1 pathogenesis, greater insights into METH’s effect on HIV-1 can be derived using such a model. Furthermore, our understanding on the molecular interplay between METH and HIV-1 will be strengthened by investigating METH’s effect on dopaminergic system of immune cells. Therefore, additional research using in vitro and in vivo models are needed to address these outstanding questions on the complex interaction between METH use and HIV-1 pathogenesis.
Abbreviations
- HIV:
-
human immunodeficiency virus
- AIDS:
-
acquired immunodeficiency syndrome
- ART:
-
antiretroviral therapy
- METH:
-
methamphetamine
- MSM:
-
men who have sex with men
- STIs:
-
sexually transmitted infections
- SIV:
-
simian immunodeficiency virus
- RNA:
-
ribonucleic acid
- CXCR4:
-
chemokine (C-X-C motif) receptor 4
- CCR5:
-
C-C chemokine receptor type 5
- ERK2:
-
extracellular-regulated kinase
- MAPK:
-
mitogen-activated protein kinase
- MDM:
-
monocyte derived macrophage
- INF- α:
-
interferon-alpha
- STAT1:
-
signal transducer and activator of transcription-1
- FIV:
-
feline immunodeficiency virus
- CNS:
-
central nervous system
- LTR:
-
Long terminal repeats
- DC-SIGN:
-
dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin
- MIP-1:
-
macrophage inflammatory protein 1
- RANTES:
-
regulated on activation T cell expressed and secreted
- VSV-G:
-
vesicular stomatitis virus g protein
- miRNA:
-
microRNA
- IL-2:
-
interlukin 2
- IFN-γ:
-
interferon-gamma
- TNF-α:
-
tumor necrosis factor alpha
- NO:
-
nitric oxide
- IL-1β:
-
interlukin beta
- IL-8:
-
interlukin 8
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Acknowledgments
Grant numbers and sources of support: This work is partly supported by grants DA024558, DA30896, DA033892 and the DIDARP grant R24DA021471 from NIDA/NIH to CD. We also acknowledge the RCMI Grant G12MD007586, the Vanderbilt CTSA grant UL1RR024975, the Meharry Translational Research Center (MeTRC) CTSA grant (U54 RR026140 from NCRR/NIH, the U54 grant MD007593 from NIMHD/NIH and the DA029506 grant that established the patient samples.
Ethical Statement
The authors declare that the manuscript has not been submitted to more than one journal for simultaneous consideration. No data have been fabricated or manipulated (including images) to support your conclusions.
Author Contributions
RC and HQ carried out search of clinical literatures. JP and CD carried literature survey of cellular and molecular studies. CD designed and directed the entire study. All authors contributed to write and review the manuscript.
Competing Interests
The authors declare no competing financial and non-financial interests.
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Passaro, R.C., Pandhare, J., Qian, HZ. et al. The Complex Interaction Between Methamphetamine Abuse and HIV-1 Pathogenesis. J Neuroimmune Pharmacol 10, 477–486 (2015). https://doi.org/10.1007/s11481-015-9604-2
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DOI: https://doi.org/10.1007/s11481-015-9604-2