Abstract
Fertilization and straw return have been widely adopted to maintain soil fertility and increase crop yields, but their long-term impacts on the accumulation and availability of cadmium (Cd) in paddy soils are still unconfirmed. Therefore, this study was undertaken in central China to investigate the accumulation, availability, and subsequent uptake of Cd by rice (Oryza sativa L.) in two adjacent field trials (P1 and P2, lasting for 10 and 12 years, respectively) under long-term straw return or in combination with chemical fertilizers. Obvious Cd accumulation, probably due to the notable Cd input from irrigation and traffic exhaust in the bulk soil (0–20 cm) of P1, was observed. The bulk soil of P2 received homogeneous straw return and chemical fertilizers, as did that of P1; however, the P2 soil almost showed Cd balance. Long-term straw return increased the portion of soil DTPA-extractable Cd to the total pool for both sites, but only P1 showed significant differences when compared to the controls. However, the highest Cd concentrations and the maximum bioconcentration factors in rice straw and grain were obtained using solo application of chemical fertilizers at both sites. Continuous additional applications of crop straw, in contrast, resulted in slightly decreased Cd uptake in rice straw, but not in grain. These findings demonstrate that neither long-term straw return nor fertilization leads directly to notable Cd accumulation, but that the promotion effects of long-term chemical fertilizer applications on Cd uptake in rice need more attention.
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Introduction
Cadmium (Cd) has been listed as the priority inorganic soil pollutant in China and worldwide due to its toxicity to organisms and nonbiodegradability in soils (Zhao et al. 2010; Chen et al. 2018a). Cd accumulation in agricultural soils, especially those used for rice production, is one of the major environmental issues attracting growing concern (Chen et al. 2018b; Zhao et al. 2015). Rapid industrialization and urbanization in China over the past several decades has accelerated the Cd input to paddy soils through atmospheric deposition, irrigation, and usage of organic manures and agrochemicals (Hu et al. 2016a; Yi et al. 2018). Moreover, the high transferability of Cd in soil–rice systems contributes to an increasing contamination risk of Cd in rice (Zhao et al. 2010; Siebers et al. 2013; Chen et al. 2018a, c). As a result, the Cd concentration in rice grains has often been reported to exceed the limit set by the Chinese government (0.2 mg kg−1 DW) (Chen et al. 2018a, b). The consumption of contaminated rice grains is a major pathway for human exposure to the highly carcinogenic Cd (Yang et al. 2017; Wang et al. 2016) and ultimately poses a potential threat to human health.
China has large amounts of straw residues derived from the continual increase in crop production (Xu et al. 2016; Yin et al. 2018). Conventional practices of dealing with the very large quantities of straw residues, such as open-air burning and throwing the straw away, are detrimental to air quality and soil fertility (Chen et al. 2017a; Yin et al. 2018). Therefore, crop straw returned to on-site fields has now been widely recommended in China for its advantages in mitigating the related environmental problems (e.g., air pollution and greenhouse gas emissions) (Li et al. 2018) and for improving soil fertility and productivity (Yang et al. 2015a; Zhu et al. 2015; Chen et al. 2017b; Zhao et al. 2018). However, crop straw commonly accumulates considerable Cd (Wang et al. 2015), and straw removal has been proven to be the primary output pathway of Cd in contaminated paddy fields (Yi et al. 2018). Meanwhile, crop straw also exhibits great potential for influencing Cd mobility and availability (Ok et al. 2011; Yang et al. 2015b; Xu et al. 2016; Tang et al. 2017). Many efforts have been made to elucidate the changes in soil Cd mobility and bioavailability after return of crop straw. The evidence has shown that the effects of crop straw return on Cd availability in soils are influenced by soil pH, soil organic carbon (SOC), availability of mineral nutrients (e.g., Ca, Zn, Fe), and Cd content in straw (Zeng et al. 2011; Wang et al. 2015). However, contradictory results have been reported. Some of the previous studies have indicated that the addition of crop straw may immobilize Cd via an increase in soil pH (Wang et al. 2015) and metal adsorption by ligands in organic matter (Mohamed et al. 2010; Xu et al. 2016; Tang et al. 2017) and in turn decrease plant Cd accumulation. Ok et al. (2011) revealed that rapeseed residue amendment decreased the easily accessible fraction of Cd by 5–14%, thereby reducing metal availability to the rice plant. Xu et al. (2016) reported that the rice straw and wheat straw addition reduced the Cd concentration in maize shoots by 69.5 and 66.9%, respectively. Zeng et al. (2019) found that the addition of crushed straw could inhibit Cd uptake by two crops in the cassava–peanut intercropping system. Other studies suggested that application of crop residues induced an increase in dissolved organic matter, thus enhancing soil Cd solubility and availability as well as the uptake of Cd by plants (Wu et al. 2012a; Bai et al. 2013). Xu et al. (2018) found that the long-term application of rice straw residue significantly increased (p < 0.05) the concentration of Cd in rice stem in double-cropping rice systems in southern China. In addition, the combined application of chemical fertilizers (mainly nitrogen, phosphorus, and potassium fertilizers) that compensated for the nutrient imbalance in straw is also a routine agricultural practice to obtain high crop yields and is an important source of Cd input to paddy soils (Jiao et al. 2012; Wang et al. 2014). For instance, continuous application of phosphate fertilizer has been identified as the main source of Cd in agricultural soils (Seshadri et al. 2016). Furthermore, applying nitrogen fertilizer was found to increase Cd uptake and accumulation in rice (Yang et al. 2016) and in wheat (Li et al. 2011). However, most of these studies were generally based on artificial soil Cd contamination and/or conducted using pots or in short-term field experiments. The results obtained from long-term field trials may be more convincing and meaningful for practical agricultural operations (Wu et al. 2012b). Therefore, the gap in information regarding Cd accumulation and availability at the field scale as influenced by long-term crop straw return or in combination with fertilization still needs to be investigated.
With a high population density and rapid industrial development, the Jianghan Plain, an area of major rice production located in central China, is confronting a growing risk of soil Cd pollution (Yi et al. 2011; Gu et al. 2016). Two adjacent field trials focusing on the effects of long-term straw return on soil quality, fertility, and productivity in a rice–wheat (Triticum aestivum L.) rotation system located on Jianghan Plain have been carried out for more than 10 years, but Cd accumulation and availability have not been examined in detail. Therefore, the aim of the present study is to shed light on the influence of long-term applications of crop straw alone or in combination with chemical fertilizers on Cd accumulation, availability, and uptake by rice plants. This research will provide valuable information for predicting Cd risk under long-term fertilization and crop straw return.
Materials and methods
Study area
The two long-term straw return fields where the trials were conducted (P1 and P2) are located in the core area of the Jianghan Plain in central China (longitude 112° 37′ E, latitude 30° 22′ N), which has a humid subtropical monsoon climate with an annual average temperature of 15.8–17.5 °C and a mean precipitation of 1250 mm concentrated from April to August. The soil is classified as an inceptisol according to the Keys to Soil Taxonomy (Soil Survey Staff 2014). The selected basic physicochemical properties of the bulk soils (0–20 cm) in the two study sites are listed in Table S1 in the Supplementary material. As illustrated in Fig. 1, P1 is close to State Road 318, which is one of the most important national highways across China. Moreover, the irrigation channel of P1 is connected with the industrial and domestic wastewater channels of a nearby town. However, P2 is at a distance from the road and is irrigated by water brought directly from a branch of Yangtze River.
Experimental design
The three treatments of P1 consisted of CK1 (control without fertilization and straw return), NPK1 (chemical fertilizers), and NPKS1 (chemical fertilizers and straw return) since the beginning of 2007, and plots (6.0 × 3.5 m2) were arranged in a randomized block design with four replicates. However, in P2, four treatments initiated in 2005, which includes S2 (straw return alone) that was added compared to those of P1, were applied to plots (5.0 × 4.0 m2) arranged in a randomized block design with four replicates. Rice–wheat rotation was designed for both sites and the rice variety belonged to the indica hybrid cultivar. In each crop season, the crop straw was harvested from 10 cm above the ground, and the stubble and root were left in the field. The straw collected from the two sites was cut into 10 cm pieces after threshing and then mixed thoroughly, and commercially available effective microorganisms (Wuhan Heyuan Green Organism Co., Ltd., China), which were mainly composed of typical microbial communities in soils (e.g., bacteria, yeasts, fermenting fungi, and actinomycetes), were added to facilitate rapid microbial decomposition of the straw for 2 to 3 weeks. For straw returned to the plots, the wheat/rice straw was uniformly incorporated into the surface soil by tilling before rice transplantation or sowing of wheat. Nitrogen, phosphorus, and potassium were applied as urea (46% N), calcium superphosphate (12% P2O5), and potassium chloride (60% K2O), respectively. Elemental analysis revealed that the Cd content in urea and calcium superphosphate was 0.01 and 0.46 mg kg−1, respectively, and Cd was undetected in potassium chloride. The application rates of chemical fertilizers or straw in the corresponding treatments are shown in Table S2. There were no other additives used, except for herbicides and pesticides for weed and pest control, respectively, during both rice and wheat seasons.
Soil and rice sampling
Rice plants were harvested and separated into straw and grain at maturity in early October 2017. Plant samples, including straw and grain, were randomly collected from each plot, washed with tap water, and then rinsed with deionized water before drying to a constant weight. Straw and grain were ground with a stainless steel mill into powder.
Five subsamples were randomly collected from the surface soil (0–20 cm) in each plot after the rice harvest and were mixed thoroughly into a 1-kg sample. The compost samples were air-dried and ground to < 2 mm for pH and bioavailable metal measurements and to < 0.149 mm for the determination of SOC, total Cd, and Cd fractionation.
Chemical analyses and quality control
For total Cd determination, approximately 0.5 g finely ground plant sample was digested with 5 ml concentrated HNO3 and 2 ml H2O2 (30%). Correspondingly, approximately 0.2 g finely ground soil sample was digested with 6 ml concentrated HNO3, 2 ml HCl, and 2 ml HF in a microwave digestion system (Milestone ETHOS UP, Italy) for 1 h. After microwave digestion, Teflon digestion tubes were heated at approximately 180 °C to allow the acids to evaporate. Eventually, the digests of both plant and soil samples were diluted and filtered through 0.45-μm membranes. As the pH of soils in our study was > 7.0, and diethylenetriaminepentaacetic acid (DTPA), as described by Lindsay and Norvell (1978), was used as extractant to determine the levels of bioavailable Fe, Mn, Cu, Zn, and Cd. Briefly, 10 g soil was shaken for 2 h in 20 ml solution containing 0.005 M DTPA, 0.01 M CaCl2, and 0.1 M triethanolamine (TEA) at pH 7.3. Soil Cd fractionation was carried out using a BCR sequential extraction method according to Yi et al. (2018), and metals were differentiated into an exchangeable and carbonate-bound fraction (F1), a Fe–Mn oxide–bound fraction (F2), an organically and sulfide-bound fraction (F3), and finally a residual fraction (F4). All of the digests and extracts above were stored in PVC bottles at 4 °C prior to analysis.
The Cd concentrations in the plant samples and in the paddy soils, as well as DTPA-extractable Cd concentrations, were determined by graphite furnace atomic absorption spectrometry (PinAAcle 900T, PerkinElmer, USA). DTPA-extractable Fe, Mn, Cu, and Zn concentrations were determined by atomic absorption spectrometry (PinAAcle 900T, PerkinElmer, USA). Replicate samples and certified reference materials (GBW07428 for total Cd in soil; GBW07413a for DTPA-Fe, DTPA-Mn, DTPA-Cu, DTPA-Zn, and DTPA-Cd; GBW07443 for Cd speciation; and GBW(E)100348 for Cd in plant samples) were included for quality assurance.
Soil pH was measured using a pH meter with a glass electrode at a soil to water ratio of 1:2.5 (w/v). SOC was determined by the K2Cr2O7–H2SO4 method (Zhao et al. 2018). In addition, the exchangeable Ca and Mg were extracted using 1 M NH4OAc at pH 7.0 (Bao 2000), and the concentrations were then determined by the atomic absorption spectrophotometer (PinAAcle 900T, PerkinElmer, USA).
Calculation of bioconcentration factor and translocation factor
In the present study, the bioconcentration factor (BCF) and the translocation factor (TF) are introduced. The BCF (also termed as the plant uptake factor, transfer factor) was commonly adopted to denote the transfer of heavy metals from soil to plant tissues (Chen et al. 2009; Ok et al. 2011; Ding et al. 2013; Yang et al. 2017) and is calculated using the following equation: BCF = Cplant tissues / Csoil, where Cplant tissues and Csoil refer to Cd concentrations (mg kg−1) in the plant tissues (straw or grain) and in the soil, respectively. The TF was calculated using the equation: TF = Cgrain / Cstraw, where Cgrain and Cstraw are the Cd concentrations (mg kg−1) in grain and straw, respectively (Gao et al. 2018; Liu et al. 2018).
Statistical analysis
One-way analysis of variance (ANOVA) was performed to evaluate the significance of the treatment effects using the LSD test (p < 0.05). Pearson correlation analysis was carried out to explore the correlations among Cd concentrations in straw and grain and soil properties as well as bioavailable metals in soils; the data included were log10 transformed (except for pH) to fulfill the normality and homogeneity assumptions of variance. All of the statistical analyses were conducted using the statistical program R version 3.1.3 (R Development Core Team 2015; http://www.r-project.org/). Graphs were prepared using Origin 9.0 (OriginLab Corp, Northampton, MA, USA).
Results
Rice growth
Both straw biomass and grain yield were significantly (p < 0.05) enhanced in the plots receiving chemical fertilizers (e.g., NPK1 and NPK2) or the combined application of chemical fertilizers and straw (e.g., NPKS1 and NPKS2) compared to their associated but unamended control plots (Fig. 2). In particular, NPKS1 and NPKS2 had the most pronounced effects on grain yield among the treatments of P1 and P2, respectively.
Changes in soil pH, SOC, and soil bioavailable metals
A significant (p < 0.05) decrease in soil pH in bulk soils of NPK1 and NPK2 was observed when compared to their corresponding controls (CK1 and CK2, respectively), and additional straw return (NPKS1 and NPKS2) slightly diminished the decrease (Table 1). The SOC content significantly (p < 0.05) improved in plots receiving straw incorporation when compared to their corresponding controls. The DTPA-extractable Fe, Cu, and Zn and exchangeable Mg contents were significantly (p < 0.05) enhanced after the application of NPKS1, relative to those of CK1. In contrast, both NPK2 and NPKS2 significantly (p < 0.05) increased the DTPA-extractable Fe content but markedly (p < 0.05) decreased the DTPA-extractable Mn content in the bulk soil from P2.
Accumulation and availability of Cd in bulk soil
Except for NPKS1, which notably (p < 0.05) increased the DTPA-extractable Cd in bulk soil, the total and DTPA-extractable Cd in the rest of the treatments for both sites did not differ significantly when compared to the corresponding controls (Fig. 3). Indeed, the total Cd in NPKS1 showed a minor accumulation when compared with that in CK1, while a slight decrease of 6.9% in total Cd was observed in NPKS2 compared with CK2. The proportion of DTPA-extractable Cd in total Cd, however, showed a uniform increase after straw was incorporated at both sites, but only with significant differences (p < 0.05) in NPKS1, relative to CK1.
Distribution of Cd fractions
The soil Cd fraction results showed that for the bulk soil of P1, the exchangeable and carbonate-bound fraction (F1) was the highest fraction, followed by the Fe–Mn oxide–bound fraction (F2), the residual fraction (F4), and the organically and sulfide-bound fraction (F3) (Fig. 4). The percentage of F1 increased from 51.1% in CK1 to 52.5% in NPK1 and to 53.6% in NPKS1. Meanwhile, the percentage of F2 increased from 31.6% in CK1 to 32.2% in NPK1 and to 33.3% in NPKS1. Correspondingly, the proportion of F3 and F4 in NPK1 and NPKS1 decreased. Similarly, F1 was also the dominant geochemical phase in bulk soils from P2. The percentages of F2 and F3 decreased from 21.04 and 7.17% in CK2 to 18.54 and 6.78% in S2, respectively, while the percentage of F4 increased from 22.98% in CK1 to 24.39% in S2. However, it was the percentage of F1 that slightly increased from 49.73% in NPK2 to 51.46% in NPKS2, and the percentage of F4 decreased from 23.98% in NPK2 to 21.89% in NPKS2.
Cd concentrations in grain and straw
Higher concentrations of Cd in rice straw and grain were observed in P1 than in the corresponding plots in P2 (Table 2), which coincided with the results of both the total Cd and DTPA-extractable Cd contents. However, the application of chemical fertilizers alone notably (p < 0.05) increased the Cd concentrations in both straw and grain for the two study sites when compared with the corresponding controls. Meanwhile, BCFstraw and BCFgrain also showed an increasing trend after the application of chemical fertilizers alone, but only with a significant difference (p < 0.05) in P2 relative to the controls. However, the promoting effects induced by the application of chemical fertilizers alone on enhancing Cd concentrations in straw and the corresponding BCFstraw value were slightly mitigated after the addition of crop straw. Furthermore, the addition of crop straw caused no significant changes in Cd concentrations in grains and in the corresponding BCFgrain value when compared to those in plots receiving chemical fertilizer application because of the elevated TFstraw–grain values at both sites.
In addition, there was an insignificant increase in Cd concentrations of straw and grain, under the S2 treatment, compared to those of CK2. Similarly, higher BCFstraw and BCFgrain were also obtained but did not show significant differences relative to CK2.
Correlation coefficients of Cd concentrations in rice straw and grain with soil pH, SOC, and bioavailable metals in the bulk soil
Pearson correlation analysis was performed to identify the influence of pH, SOC, and soil bioavailable metals on Cd concentrations in rice straw and grain (Table 3). It can be seen that soil pH was significantly and negatively associated with the logarithmic values of Cd in rice straw and in grain in P2. In contrast, it was the SOC content that significantly and positively correlated with the logarithmic values of Cd in rice straw and in grain in P1. These results suggested that SOC and pH played a major role in influencing Cd uptake in P1 and P2, respectively.
In addition, the correlations between the logarithmic values of Cd concentrations in rice straw or grain and the logarithmic values of bioavailable metal content in bulk soil were positive at p < 0.05 for Fe, negative at p < 0.05 for Mn and Ca, and not significant for Cu, Zn, and Mg in P1. Similar results were also found in terms of Fe (p < 0.001), Mn (p < 0.001), and Ca (p < 0.05) in P2, where a significantly negative correlation (p < 0.05) between the logarithmic values of Cd concentration in straw or grain and soil Mg content was also found.
Discussion
Effects of long-term fertilization and crop straw return on Cd accumulation and bioavailability in the bulk soil
Previous studies documented that total Cd content in farmland soils was determined by both parent materials and anthropogenic activities (e.g., atmospheric deposition, irrigation, and application of chemical fertilizers and agrochemicals) (Wang et al. 2014; Yi et al. 2018). In the present study, the P1 site is close to a major national highway with heavy traffic and its irrigation channel runs through a residential area (Fig. 1), and the total Cd content at the initial sampling in 2007 in P1 was 0.49 mg kg−1 (Table S1) and rose to 0.63 mg kg−1 in control plots after the rice harvest in 2017(Fig. 3), which is higher than the risk screening values for soil contamination of agricultural land (0.6 mg kg−1, pH > 7.5) set by the Chinese soil environmental quality standard GB15618-2018. It was obvious that notable Cd accumulation had occurred in bulk soils of the controls in P1, which was presumably due to the substantial amount of Cd input from the polluted irrigation water or from nearby traffic discharges. The Cd input from irrigation water could be indirectly reflected by the Cd content in irrigation channel sediments acting as a sink for Cd (Ghazban et al. 2015). One such investigation of the distribution of heavy metals in irrigation channel sediments of the same area was conducted in 2013 by Gu et al. (2016) and revealed that the mean content of total Cd for 36 sampling sites was 1.79 mg kg−1 (Table S1), which is significantly higher than the background value (0.17 mg kg−1) and our results (Fig. 3) and implies that the irrigation water had once been polluted by Cd. In addition, higher contamination levels of Cd at the roadside sampling sites were detected during the same investigation. Therefore, more attention should be paid to the Cd input from irrigation or traffic discharges in P1. In contrast, the total Cd content in the control plots of P2 remained relatively constant at approximately 0.26 mg kg−1 after 12-year fertilization or straw return (Table S1 and Fig. 3). Moreover, there were no significant differences in both total Cd and DTPA-extractable Cd contents among all treatments of P2, where some of the primary input pathways of Cd (e.g., chemical fertilizers, straw return, and agrochemicals) had the same sources as those in P1. It is suggested that in the present study the application of crop straw as well as chemical fertilizers themselves did not result in a distinct accumulation risk of soil Cd. Consistent results were found by other researchers (Wu et al. 2012b; Zhou et al. 2015; Xu et al. 2018).
Generally, the mobility and phytoavailability of Cd is associated with soil properties (e.g., pH, SOC, cation exchange capacity, and oxidation–reduction status), among which soil pH and SOC content posed more distinct effects on Cd mobility (Zhao et al. 2010; Zeng et al. 2011; Ding et al. 2013; Meng et al. 2018; Xu et al. 2018), thereby influencing the Cd uptake by rice plants (Xiao et al. 2017). In our study, notable change in soil pH occurred in plots receiving chemical fertilizers, and it was the SOC content that significantly (p < 0.05) increased after continuous straw return at both the P1 and P2 sites (Table 1). Our results showed that it was the soil pH, rather than SOC, that significantly (p < 0.05) and negatively correlated with the logarithmic values of Cd in the rice straw and in grain in P2 (Table 3), which suggests that soil pH plays a more important role in controlling Cd availability in the soils of P2 which were almost under Cd balance. However, a significant (p < 0.05) negative relationship between the logarithmic values of Cd concentrations in grain and the DTPA-Cd content in bulk soil was obtained in P2 (Table 3), which may be explained by the slight decrease in DTPA-extractable Cd in the bulk soil of P2 (Fig. 3) that resulted from the higher rice plant biomass and higher Cd concentrations in rice aerial tissues and, thus, higher uptake amounts of Cd from soil in the plots receiving long-term applications of chemical fertilizers. In contrast, the only significant positive relationship (p < 0.05) between SOC and the logarithmic values of Cd in the rice straw and grain was observed in P1, thus demonstrating a more important role for SOC in influencing Cd phytoavailability in the soils of P1 with substantial amounts of Cd input. The proportion of DTPA-extractable Cd in total Cd increased significantly (p < 0.05) in NPKS1 (Fig. 3), which is consistent with the elevated percentage of the exchangeable and carbonate-bound fraction and the Fe–Mn oxide–bound fraction compared with those of NPK1 (Fig. 4). Furthermore, a significant relationship (R2 = 0.45, p < 0.05) between SOC and DTPA-extractable Cd content was observed in P1, which indicates that the role of SOC in affecting Cd availability may be ascribed to the formation of SOC–metal complexes (Liu et al. 2009; Zeng et al. 2011; Kwiatkowska-Malina 2018). Many researchers have also found that low molecular weight organic matter introduced by decomposition of straw might act as a carrier of Cd (Khokhotva and Waara 2010; Wu et al. 2012a; Bai et al. 2013; Xie et al. 2019).
Effects of long-term fertilization and crop straw return on rice growth, Cd uptake, and translocation in rice tissues
Rice growth was clearly enhanced with the addition of crop straw, let alone chemical fertilizers and their combined application when compared to the long-term nonfertilized controls at both sites (Fig. 2), which was in accordance with a number of previous studies (Hu et al. 2016b; Xu et al. 2010; Li et al. 2014; Zhang et al. 2015) and indicates that straw return is a feasible practice in terms of maintaining rice productivity.
The Cd concentrations in rice grain harvested from both P1 and P2 did not exceed the limit set by the Chinese government, which may be attributable to the alkaline characteristics of the soils in both P1 and P2. However, the application of chemical fertilizers with or without straw return was significantly (p < 0.05) effective in facilitating Cd uptake and accumulation in both rice straw and grain from the two sites, and the highest Cd concentrations in grain were achieved with the solo application of chemical fertilizers (Table 2); this finding is consistent with the results of Singh et al. (2010), thus demonstrating the Cd accumulation risk in rice grain under long-term application of chemical fertilizers. In contrast, additional straw incorporation resulted in higher proportions of DTPA-extractable Cd in total Cd at both sites, but with a slight decrease in Cd accumulation in rice straw. An important reason for this inconsistency is likely the competition for absorption between Cd and other mineral nutrients. As a nonessential metal ion, Cd2+ was absorbed and transported in rice plants by exploiting the transport systems for essential cations such as Ca2+, Mn2+, and Zn2+ which are competitors of Cd2+ for adsorption sites (Seshadri et al. 2016; Liu et al. 2017). The observation that the logarithmic values of DTPA-extractable Mn and exchangeable Ca showed significant negative correlations with the logarithmic values of Cd concentrations in rice straw or grain at both sites (Table 3) may confirm the above speculation. However, there was no reduction in Cd concentration in grain as was seen for rice straw after the additional straw return. This is predominantly because of the slightly increased TFstraw–grain induced by additional straw incorporation (Table 2) which is inconsistent with the previous study of Xu et al. (2018), who found that the Cd concentrations in rice stems increased significantly but decreased significantly in rice grain under long-term combined application of rice straw and chemical fertilizers compared to that of the chemical fertilizer applications. These results may imply that the long-term straw return practices will at least not promote Cd accumulation in rice grain. However, it has been reported that transportation of Cd from root to straw and not straw to grain is likely the major process determining Cd accumulation in rice grain (Uraguchi et al. 2009; Gao et al. 2018), and the reduction of Cd enrichment in rice straw usually results in lower Cd accumulation in rice grain. Therefore, further studies should be conducted to elucidate the underlying mechanism of the elevated TFstraw–grain after additional straw incorporation.
Conclusion
At two adjacent long-term experimental sites, the plots receiving long-term application of straw return and/or chemical fertilizers from the same sources for 10 or 12 years were under different Cd balance. Only the bulk soil at P1 showed distinct Cd accumulation, which was probably derived from irrigation and traffic discharges, and the elevated SOC content induced by straw incorporation was further beneficial for Cd accumulation. The SOC and soil pH played a major role in controlling Cd availability in the soils of P1 and P2, respectively. Despite the long-term application of straw return with or without chemical fertilizers, the proportion of DTPA-extractable Cd in total Cd increased slightly at both sites compared with untreated controls or with plots receiving chemical fertilizers; the application of chemical fertilizers was more effective in facilitating Cd uptake in rice straw and grain. Additional straw return could slightly decrease the Cd concentrations in rice straw, but not in grain, and the mechanisms involved need further investigation. Overall, long-term application of chemical fertilizers and crop straw return have no obvious impacts on Cd accumulation in the bulk soil, but we should take note of the substantial amount of Cd input from other sources and the promotion effects of long-term application of chemical fertilizers on Cd uptake in rice.
References
Bai YC, Gu CH, Tao TY, Chen GH, Shan YH (2013) Straw incorporation increases solubility and uptake of cadmium by rice plants. Acta Agr Scand BSP 63:193–199. https://doi.org/10.1080/09064710.2012.743582
Bao S (2000) Soil agrochemical analysis, 3rd edn. Agricultural Press, Beijing (in Chinese)
Chen WP, Li LQ, Chang AC, Wu LS, Chaney RL, Smith R, Ajwa H (2009) Characterizing the solid–solution partitioning coefficient and plant uptake factor of As, Cd, and Pb in California croplands. Agric Ecosyst Environ 129:212–220. https://doi.org/10.1016/j.agee.2008.09.001
Chen JM, Li CL, Ristovski Z, Milic A, Gu YT, Islam MS, Wang SX, Hao JM, Zhang HF, He CR, Guo H, Fu HB, Miljevic B, Morawska L, Thai P, Lam YF, Pereira G, Ding AJ, Huang X, Dumka U (2017a) A review of biomass burning: emissions and impacts on air quality, health and climate in China. Sci Total Environ 579:1000–1034. https://doi.org/10.1016/j.scitotenv.2016.11.025
Chen ZM, Wang HY, Liu XW, Zhao XL, Lu DJ, Zhou JM, Li CZ (2017b) Changes in soil microbial community and organic carbon fractions under short-term straw return in a rice-wheat cropping system. Soil Tillage Res 165:121–127. https://doi.org/10.1016/j.still.2016.07.018
Chen HP, Tang Z, Wang P, Zhao FJ (2018a) Geographical variations of cadmium and arsenic concentrations and arsenic speciation in Chinese rice. Environ Pollut 23:482–490. https://doi.org/10.1016/j.envpol.2018.03.048
Chen HP, Zhang WW, Yang XP, Wang P, McGrath SP, Zhao FJ (2018b) Effective methods to reduce cadmium accumulation in rice grain. Chemosphere 207:699–707. https://doi.org/10.1016/j.chemosphere.2018.05.143
Chen HP, Yang XP, Wang P, Wang ZX, Li M, Zhao FJ (2018c) Dietary cadmium intake from rice and vegetables and potential health risk: a case study in Xiangtan, southern China. Sci Total Environ 639:271–277. https://doi.org/10.1016/j.scitotenv.2018.05.050
Ding CF, Zhang TL, Wang XX, Zhou F, Yang YR, Huang GF (2013) Prediction model for cadmium transfer from soil to carrot (Daucus carota L.) and its application to derive soil thresholds for food safety. J Agric Food Chem 61:10273–10282. https://doi.org/10.1021/jf4029859
Gao M, Zhou J, Liu HL, Zhang WT, Hu YM, Liang JN, Zhou J (2018) Foliar spraying with silicon and selenium reduces cadmium uptake and mitigates cadmium toxicity in rice. Sci Total Environ 631:1100–1108. https://doi.org/10.1016/j.scitotenv.2018.03.047
Ghazban F, Parizanganeh A, Zamani A, Taghilou B (2015) Assessment of heavy metal pollution in water and sediments from the Ghalechay River, Baychebaghcopper mine area, Iran. Soil Sediment Contam 24:172–190. https://doi.org/10.1080/15320383.2014.937391
Gu CM, Liu Y, Liu DB, Li ZG, Mohamed I, Zhang RH, Brooks M, Chen F (2016) Distribution and ecological assessment of heavy metals in irrigation channel sediments in a typical rural area of south China. Ecol Eng 90:466–472. https://doi.org/10.1016/j.ecoleng.2016.01.054
Hu YN, Cheng HF, Tao S (2016a) The challenges and solutions for cadmium-contaminated rice in China: a critical review. Environ Int 92:515–532. https://doi.org/10.1016/j.envint.2016.04.042
Hu NJ, Wang BJ, Gu ZH, Tao BR, Zhang ZW, Hu SJ, Zhu LQ, Meng YL (2016b) Effects of different straw returning modes on greenhouse gas emissions and crop yields in a rice-wheat rotation system. Agric Ecosyst Environ 223:115–122. https://doi.org/10.1016/j.agee.2016.02.027
Jiao WT, Chen WP, Chang AC, Page AL (2012) Environmental risks of trace elements associated with long-term phosphate fertilizers applications: a review. Environ Pollut 168:44–53. https://doi.org/10.1016/j.envpol.2012.03.052
Khokhotva O, Waara S (2010) The influence of dissolved organic carbon on sorption of heavy metals on urea-treated pine bark. J Hazard Mater 173:689–696. https://doi.org/10.1016/j.jhazmat.2009.08.149
Kwiatkowska-Malina J (2018) Functions of organic matter in polluted soils: the effect of organic amendments on phytoavailability of heavy metals. Appl Soil Ecol 123:542–545. https://doi.org/10.1016/j.apsoil.2017.06.021
Li XL, Ziadi N, Bélanger G, Cai ZC, Xu H (2011) Cadmium accumulation in wheat grain as affected by mineral N fertilizer and soil characteristics. Can J Soil Sci 91:521–531. https://doi.org/10.4141/cjss10061
Li M, Tian X, Liu RZ, Chen WL, Cai P, Rong XM, Dai K, Huang QY (2014) Combined application of rice straw and fungus Penicillium chrysogenum to remediate heavy-metal-contaminated soil. Soil Sediment Contam Int J 23:328–338. https://doi.org/10.1080/15320383.2014.827623
Li H, Dai MW, Dai SL, Dong XJ (2018) Current status and environment impact of direct straw return in China’s cropland—a review. Ecotoxicol Environ Saf 159:293–300. https://doi.org/10.1016/j.ecoenv.2018.05.014
Lindsay WL, Norvell WA (1978) Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Sci Soc Am J 42:421–428
Liu LN, Chen HS, Cai P, Liang W, Huang QY (2009) Immobilization and phytotoxicity of Cd in contaminated soil amended with chicken manure compost. J Hazard Mater 163:563–567. https://doi.org/10.1016/j.jhazmat.2008.07.004
Liu Y, Zhang CB, Zhao YL, Sun SJ, Liu ZQ (2017) Effects of growing seasons and genotypes on the accumulation of cadmium and mineral nutrients in rice grown in cadmium contaminated soil. Sci Total Environ 579:1282–1288. https://doi.org/10.1016/j.scitotenv.2016.11.115
Liu L, Li JW, Yue FX, Yan XW, Wang FY, Bloszies S, Wang YF (2018) Effects of arbuscular mycorrhizal inoculation and biochar amendment on maize growth, cadmium uptake and soil cadmium speciation in Cd-contaminated soil. Chemosphere 194:495–503. https://doi.org/10.1016/j.chemosphere.2017.12.025
Meng J, Zhong LB, Wang L, Liu XM, Tang CX, Chen HJ, Xu JM (2018) Contrasting effects of alkaline amendments on the bioavailability and uptake of Cd in rice plants in a Cd-contaminated acid paddy soil. Environ Sci Pollut Res 25:8827–8835. https://doi.org/10.1007/s11356-017-1148-y
Mohamed I, Ahamadou B, Li M, Gong C, Cai P, Liang W, Huang QY (2010) Fractionation of copper and cadmium and their binding with soil organic matter in a contaminated soil amended with organic materials. J Soils Sediments 10(6):973–982. https://doi.org/10.1007/s11368-010-0199-1
Ok YS, Usman AR, Lee SS, El-Azeem SAA, Choi B, Hashimoto Y, Yang JE (2011) Effects of rapeseed residue on lead and cadmium availability and uptake by rice plants in heavy metal contaminated paddy soil. Chemosphere 85:677–682. https://doi.org/10.1016/j.chemosphere.2011.06.073
R Development Core Team (2015) R: a Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria
Seshadri B, Bolan NS, Wijesekara H, Kunhikrishnan A, Thangarajan R, Qi F, Matheyarasu R, Rocco C, Kenneth M, Naidu R (2016) Phosphorus–cadmium interactions in paddy soils. Geoderma 270:43–59. https://doi.org/10.1016/j.geoderma.2015.11.029
Siebers N, Siangliw M, Tongcumpou C (2013) Cadmium uptake and subcellular distribution in rice plants as affected by phosphorus: soil and hydroponic experiments. J Soil Sci Plant Nutr 13:833–844. https://doi.org/10.4067/S0718-95162013005000066
Singh A, Agrawal M, Marshall FM (2010) The role of organic vs. inorganic fertilizers in reducing phytoavailability of heavy metals in a wastewater-irrigated area. Ecol Eng 36:1733–1740. https://doi.org/10.1016/j.ecoleng.2010.07.021
Soil Survey Staff (2014) Keys to soil taxonomy, 12th edn. United States Department of Agriculture, Washington D.C.
Tang WL, Zhong H, Xiao L, Tan QG, Zeng QL, Wei ZB (2017) Inhibitory effects of rice residues amendment on Cd phytoavailability: a matter of Cd-organic matter interactions? Chemosphere 186:227–234. https://doi.org/10.1016/j.chemosphere.2017.07.152
Uraguchi S, Mori S, Kuramata M, Kawasaki A, Arao T, Ishikawa S (2009) Root-to-shoot Cd translocation via the xylem is the major process determining shoot and grain cadmium accumulation in rice. J Exp Bot 60:2677–2688. https://doi.org/10.1093/jxb/erp119
Wang QY, Zhang JB, Zhao BZ, Xin XL, Zhang CZ, Zhang HL (2014) The influence of long-term fertilization on cadmium (Cd) accumulation in soil and its uptake by crops. Environ Sci Pollut Res 21:10377–10385. https://doi.org/10.1007/s11356-014-2939-z
Wang S, Huang DY, Zhu QH, Zhu HH, Liu SL, Luo ZC, Cao XL, Wang JY, Rao ZX, Shen X (2015) Speciation and phytoavailability of cadmium in soil treated with cadmium-contaminated rice straw. Environ Sci Pollut Res 22:2679–2686. https://doi.org/10.1007/s11356-014-3515-2
Wang ME, Chen WP, Peng C (2016) Risk assessment of Cd polluted paddy soils in the industrial and township areas in Hunan, southern China. Chemosphere 144:346–351. https://doi.org/10.1016/j.chemosphere.2015.09.001
Wu LH, Li Z, Akahane I, Liu L, Han CL, Makino T, Luo YM, Christie P (2012a) Effects of organic amendments on Cd, Zn and Cu bioavailability in soil with repeated phytoremediation by Sedum plumbizincicola. Int J Phytorem 14:1024–1038. https://doi.org/10.1080/15226514.2011.649436
Wu LH, Tan CY, Liu L, Zhu P, Peng C, Luo YM, Christie P (2012b) Cadmium bioavailability in surface soils receiving long-term applications of inorganic fertilizers and pig manure. Geoderma 173:224–230. https://doi.org/10.1016/j.geoderma.2011.12.003
Xiao L, Guan DS, Peart MR, Chen YJ, Li QQ, Dai J (2017) The influence of bioavailable heavy metals and microbial parameters of soil on the metal accumulation in rice grain. Chemosphere 185:868–878. https://doi.org/10.1016/j.chemosphere.2017.07.096
Xie J, Dong AQ, Liu J, Su JP, Hu P, Xu CX, Chen JR, Wu QT (2019) Relevance of dissolved organic matter generated from green manuring of Chinese milk vetch in relation to water-soluble cadmium. Environ Sci Pollut R:1–13. https://doi.org/10.1007/s11356-019-05114-0
Xu YZ, Nie LX, Buresh RJ, Huang JL, Cui KH, Xu B, Gong WH, Peng SB (2010) Agronomic performance of late-season rice under different tillage, straw, and nitrogen management. Field Crop Res 115:79–84. https://doi.org/10.1016/j.fcr.2009.10.005
Xu P, Sun CX, Ye XZ, Xiao WD, Zhang Q, Wang Q (2016) The effect of biochar and crop straws on heavy metal bioavailability and plant accumulation in a Cd and Pb polluted soil. Ecotoxicol Environ Saf 132:94–100. https://doi.org/10.1016/j.ecoenv.2016.05.031
Xu YL, Tang HM, Liu TX, Li YF, Huang XJ, Pi J (2018) Effects of long-term fertilization practices on heavy metal cadmium accumulation in the surface soil and rice plants of double-cropping rice system in southern China. Environ Sci Pollut R 25(20):19836–19844. https://doi.org/10.1007/s11356-018-2175-z
Yang HS, Yang B, Dai YJ, Xu MM, Koide RT, Wang XH, Liu J, Bian XM (2015a) Soil nitrogen retention is increased by ditch-buried straw return in a rice-wheat rotation system. Eur J Agron 69:52–58. https://doi.org/10.1016/j.eja.2015.05.005
Yang JX, Wang LQ, Li JM, Wei DP, Chen SB, Guo QJ, Ma YB (2015b) Effects of rape straw and red mud on extractability and bioavailability of cadmium in a calcareous soil. Front Environ Sci Eng 9:419–428. https://doi.org/10.1016/j.jhazmat.2010.09.082
Yang YJ, Xiong J, Chen RJ, Fu GF, Chen TT, Tao LX (2016) Excessive nitrate enhances cadmium (Cd) uptake by up-regulating the expression of OsIRT1 in rice (Oryza sativa). Environ Exp Bot 122:141–149. https://doi.org/10.1016/j.envexpbot.2015.10.001
Yang Y, Wang ME, Chen WP, Li YL, Peng C (2017) Cadmium accumulation risk in vegetables and rice in southern China: insights from solid-solution partitioning and plant uptake factor. J Agric Food Chem 65:5463–5469. https://doi.org/10.1021/acs.jafc.7b01931
Yi YJ, Yang ZF, Zhang SH (2011) Ecological risk assessment of heavy metals in sediment and human health risk assessment of heavy metals in fishes in the middle and lower reaches of the Yangtze River basin. Environ Pollut 159:2575–2585. https://doi.org/10.1016/j.envpol.2011.06.011
Yi KX, Fan W, Chen JY, Jiang SH, Huang SJ, Peng L, Zeng QR, Luo S (2018) Annual input and output fluxes of heavy metals to paddy fields in four types of contaminated areas in Hunan Province, China. Sci Total Environ 634:67–76. https://doi.org/10.1016/j.scitotenv.2018.03.294
Yin HJ, Zhao WQ, Li T, Cheng XY, Liu Q (2018) Balancing straw returning and chemical fertilizers in China: role of straw nutrient resources. Renew Sust Energ Rev 81:2695–2702. https://doi.org/10.1016/j.rser.2017.06.076
Zeng FR, Ali S, Zhang HT, Ouyang YN, Qiu BY, Wu FB, Zhang GP (2011) The influence of pH and organic matter content in paddy soil on heavy metal availability and their uptake by rice plants. Environ Pollut 159:84–91. https://doi.org/10.1016/j.envpol.2010.09.019
Zeng LP, Lin XK, Zhou F, Qin JH, Li HS (2019) Biochar and crushed straw additions affect cadmium absorption in cassava-peanut intercropping system. Ecotoxicol Environ Saf 167:520–530. https://doi.org/10.1016/j.ecoenv.2018.10.003
Zhang L, Zheng JC, Chen LG, Shen MX, Zhang X, Zhang MQ, Bian XM, Zhang J, Zhang WJ (2015) Integrative effects of soil tillage and straw management on crop yields and greenhouse gas emissions in a rice-wheat cropping system. Eur J Agron 63:47–54. https://doi.org/10.1016/j.eja.2014.11.005
Zhao KL, Liu XM, Xu JM, Selim H (2010) Heavy metal contaminations in a soil-rice system: identification of spatial dependence in relation to soil properties of paddy fields. J Hazard Mater 181:778–787. https://doi.org/10.1016/j.jhazmat.2010.05.081
Zhao FJ, Ma YB, Zhu YG, Tang Z, McGrath SP (2015) Soil contamination in China: current status and mitigation strategies. Environ Sci Technol 49:750–759. https://doi.org/10.1021/es5047099
Zhao HL, Shar AG, Li S, Chen YL, Shi JL, Zhang XY, Tian XH (2018) Effect of straw return mode on soil aggregation and aggregate carbon content in an annual maize-wheat double cropping system. Soil Tillage Res 175:178–186. https://doi.org/10.1016/j.still.2017.09.012
Zhou SW, Liu J, Xu MG, Lv JL, Sun N (2015) Accumulation, availability, and uptake of heavy metals in a red soil after 22-year fertilization and cropping. Environ Sci Pollut Res 22:15154–15163. https://doi.org/10.1007/s11356-015-4745-7
Zhu LQ, Hu NJ, Zhang ZW, Xu JL, Tao BR, Meng YL (2015) Short-term responses of soil organic carbon and carbon pool management index to different annual straw return rates in a rice-wheat cropping system. Catena 135:283–289. https://doi.org/10.1016/j.catena.2015.08.008
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The authors thank the reviewers of the manuscript for their helpful comments.
Funding
We gratefully thank the National Key Research and Development Program of China (Grant Nos. 2017YFD0801003; 2016YFD0200807), Major Research and Development Project of Hubei Academy of Agricultural Sciences (No. 2017CGPY01), Youth Foundation of Hubei Academy of Agricultural Sciences (No. 2018NKYJJ05), and Non-profit Collaborative Innovation Alliance Project (No. 2018LM) for financial support of this research.
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Nie, X., Duan, X., Zhang, M. et al. Cadmium accumulation, availability, and rice uptake in soils receiving long-term applications of chemical fertilizers and crop straw return. Environ Sci Pollut Res 26, 31243–31253 (2019). https://doi.org/10.1007/s11356-019-05998-y
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DOI: https://doi.org/10.1007/s11356-019-05998-y