Abstract
Contamination levels and spatial and temporal distributions of six typical synthetic musks (SMs) in water and sediment of the Songhua River in Northeastern China were investigated. Experimental data for 72 water and 52 sediment samples collected at 29 sampling sites over 12 months spanning 2011–2012 showed that the Songhua River had been contaminated to different degrees at various sites separately from the river’s source. The polycyclic musks 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-(g)-2-benzopyran (HHCB) (Galaxolide) and 7-acetyl-1,1,3,4,4,6-hexamethyl-1,2,3,4-tetrahydronaphthalene (AHTN) (Tonalide) were found most frequently and at the highest levels. Concentrations of HHCB were <2–37 ng/L in water and <0.5–17.5 ng/g dry weight (dw) in sediment. AHTN was <1–8 ng/L in water and <0.5–5.7 ng/g dw in sediment. Statistical relationships between SM concentrations and four environmental variables (temperature, illumination, runoff, and population density) in the Songhua River Basin were formulated. Concentration levels varied proportionately with the size of the city along the river, while the distribution patterns showed clear seasonal variations. HHCB/AHTN ratios mirrored the transfer and transmitting process of SMs. Concentrations of target compounds were correlated with each other, suggesting similar exposure sources. Environmental risk assessment of SMs presented seasonal variations and provided baseline information on SM exposure in the Songhua River Basin.
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Introduction
Synthetic musks (SMs) as emerging persistent organic pollutants are receiving increasing concern due to their potential environmental impacts (Heberer 2002; Tanabe 2005; Peck et al. 2006; Liu et al. 2013). SMs are used extensively as fragrant ingredients in a variety of scented household and personal care products (Balk and Ford 1999a; Rimkus, 2004; Lignell et al. 2008; Zhang et al. 2008; Lu et al. 2011). The most widely used SMs include polycyclic musks—1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-(g)-2-benzopyran (HHCB) (Galaxolide), 7-acetyl-1,1,3,4,4,6-hexamethyl-1,2,3,4-tetrahydronaphthalene (AHTN) (Tonalide), 4-acetyl-1,1-dimethyl-6-tert-butylindan (ADBI) (Celestolide), and 6-acetyl-1,1,2,3,3,5-hexamethylindan (AHMI) (Phantolide)—and nitro musks—1-tert-butyl-3,5-dimethyl-2,4,6-trinitrobenzene (musk xylene (MX)) and 4-acetyl-1-tert-butyl-3,5-dimethyl-2,6-dinitrobenzene (musk ketone (MK)) (Rimkus, 2004; Peck et al. 2006; Zhang et al. 2008).
Most SMs are discharged into sewage treatment plants (STPs) (Bester 2005; Yang and Metcalfe 2006). Due to continuous use and incomplete removal during the treatment processes, SMs are steadily introduced into the environment via STP effluent and sludge (Zeng et al. 2005; Yang and Metcalfe 2006; Zhang et al. 2008). Though used at low concentrations, consumption of large volumes and lengthy environmental persistence contribute to SM detection in almost every environmental compartment (Lv et al. 2009). Since the first detection of MX and MK in freshwater fish from Japan (Yamagishi et al. 1981), these compounds have been investigated in various environmental matrices, including sewage influent and effluent (Lv et al. 2009), sewage sludge (Zeng et al. 2005), surface water (Hu et al. 2011), suspended particulate matter (Dsikowitzky et al. 2002), sediment (Wang et al. 2010), soils (Chase et al. 2012), air (Peck and Hornbuckle 2004), precipitation (Hu et al. 2012), dust (Liu et al. 2013), and groundwater and drinking water (Benotti et al. 2009). As SMs are hydrophobic and lipophilic (Tanabe 2005; Schramm et al. 1996), they tend to accumulate in various biological matrices such as fish (Reiner and Kannan 2011), crustaceans and bivalves (Nakata et al. 2012), marine mammals (Kannan et al. 2005), plant tissues (Chase et al. 2012), earthworms (Rimkus, 2004), and birds (Kannan et al. 2005). In addition, SMs have been detected in human tissues (Kannan et al. 2005), blood (Hu et al. 2010), and breast milk (Lignell et al. 2008).
The ubiquitous occurrence (Peck et al. 2006), relative persistence (Tanabe 2005), bioconcentration tendency, and potential toxicological effects (carcinogenicity, genotoxicity, mutagenicity, physiological ecotoxicity, and environmental hormone toxicity [Schramm et al. 1996]) raise significant concerns about the distribution and fate of SMs in the aquatic environment. Studies have been conducted in developed countries and regions such as the European Union (Sumner et al. 2010), USA (Reiner and Kannan 2011), and Korea (Lee et al. 2010; Yoon et al. 2010); however, little information is available for developing countries (Zeng et al. 2008; Hu et al. 2011). With increased economic development, the usage volume of SMs in these regions has increased (Liu et al. 2013; Wang et al. 2013). Although the SM usage patterns in developing countries are largely unknown, these patterns are suspected to be very different from those in developed nations (Villa et al. 2012). Moreover, in developing countries, sewage treatment is only partial or, in many places, completely lacking (Liu et al. 2013; Wang et al. 2013). To clarify the global transport, distribution, and fate of SMs in the natural environment, SM monitoring in developing countries should be increased (Nakata et al. 2012).
Preliminary investigations have shown that environmental variables can affect spatial and temporal distributions of SMs in the aquatic environment (Fono et al. 2006; Peck et al. 2006; Zeng et al. 2008; Wang et al. 2010; Hu et al. 2011). However, most of these studies are limited to a very short time period or cover only a few sampling sites, some of which are uncorrelated (Quednow and Püttmann 2008; Sumner et al. 2010; Gómez et al. 2012; Villa et al. 2012). Limited data are available for large-scale and long-term monitoring of an entire river basin, especially in developing countries (Dsikowitzky et al. 2002; Bester 2005). Furthermore, little is known about the statistical relationships between SM levels and environmental variables.
As the third largest river in China, the Songhua River covers a watershed area of 556,800 km2 and has 66 million inhabitants with a population density that varies greatly along the river (Wang et al. 2012, 2013). It is an important flowing freshwater body intensively utilized not only for commercial fisheries but also for water supply, industrial production, and agricultural irrigation (Wang et al. 2012). In rural areas and some urban areas, raw sewage is discharged into the Songhua River directly due to the lack of wastewater gathering and treatment systems, while in urban areas, untreated wastewater is discharged into the river directly during heavy rains. The river is also affected by its polluted tributaries to some extent (Wang et al. 2012). Recently, the increased emission intensity has contributed to aggravated pollution levels (Wang et al. 2012).
The Songhua River Basin is located at the joint section of a temperate zone and a cold temperate zone with the greatest temperature variation (−38.1 to 39.2 °C) in China. As a typical semi-moist, continental monsoon climate region, it has a long cold winter, a rainy torrid summer, and a windy dry spring. Precipitation is concentrated from June to September, which accounts for 85 % of annual rainfall in this basin (Wang et al. 2013). For approximately 5 months annually, the river is covered with ice and snow (from mid-November to early April) with episodes of severe organic pollution each winter (Wang et al. 2012).
The Chinese Ministry of Environmental Protection has focused significantly on emerging persistent organic pollutants in the Songhua River following the Songhua River benzene spill in December 2005 (Wang et al. 2013). Densely distributed monitoring sections have been established, and an extensive monitoring database (including organic properties, physical and chemical properties, nutrients, inorganic constituents, and heavy metals) has been put in place through the ministry’s programs (Wang et al. 2013). A recent screening survey showed that SM concentrations in human blood in Harbin were the highest in China (Hu et al. 2010).
The monitoring of SMs and determination of their fate in the Songhua River Basin deserve considerable attention. While it is of great significance to study the occurrence, fate, transport, and risk assessment of SMs in this river, no information has been available until this study. The primary objectives of this study were to (1) investigate baseline contamination levels and spatial and temporal distributions of SMs in the Songhua River, (2) analyze main correlative factors (runoff, temperature, illumination, and population density) affecting SM pollution in dissolved phase (water) and sediments, (3) investigate the relationships among different ingredients and different matrices, and (4) evaluate the potential risk to the environment of SMs during four seasons. These results will help fill information gaps regarding the potential sources and long-term fate of SMs in large-scale rivers.
Materials and methods
Chemicals and materials
Six SMs were selected to survey based on levels of use in China, reported aquatic toxicity effects, and the ability to analyze for the compounds at low levels (Hu et al. 2010; Liu et al. 2013). The musk standards for HHCB, AHTN, ADBI, AHMI, MX, and MK were purchased from Promochem, Germany. All reagents were 99 % pure (GC), except HHCB with purity of 75 % (GC). Hexamethylbenzene (HMB) and d3-AHTN were obtained from Dr. Ehrenstorfer, Germany.
All solvents (dichloromethane, n-hexane, and methanol) were of high-performance liquid chromatography (HPLC) grade and were obtained from Dikma, China. The C18 columns used in this study were purchased from Supelco, USA. Glass fiber filters (GF/F, average retention diameter 47 mm), anhydrous sodium sulfate, neutral Al2O3 (100–200 mesh), and silica gel (80–100 mesh) were baked at 450 °C for 4 h prior to use.
Sampling
As shown in Fig. 1, nine key sampling sites (S1 to S9) and 20 auxiliary sampling sites (AS1 to AS20) were selected for this study. Water and bottom sediment samples were collected in the middle of 12 months spanning 2011–2012. The detailed sampling program is listed in the supplementary materials.
The equal-width-increment (EWI) method (US Geological Survey 2006) was employed to collect the isokinetic, depth-integrated samples at each stream cross section sampled (see Fig. 1). After collection, mixed water samples were immediately transferred to precleaned 5-L amber glass bottles and sealed (free of air bubbles) using glass stoppers. Samples were kept on ice during transit to the laboratory, preserved by the addition of 0.5 % methanol (v/v) and stored in the dark at 4 °C until extraction. All samples were extracted within 10 days of collection. Relevant data for river runoff were supplied by the Harbin Station of Environmental Monitoring, Heilongjiang Province, China.
A bottom-sediment sampling location was located just below each water sampling location (shown in Fig. 1). At each sampling point in the transversal section of the river, the top sediment samples (a mixture of sediments from the upper 10 cm shown in Fig. 1) were collected by a box-like grab sampler (manufactured by the Harbin Station of Environmental Monitoring, Heilongjiang Province, China). After sampling, the top 5 cm of sediment was transferred using a precleaned, stainless steel scoop into precleaned 1-L amber glass containers. The collected samples were then homogenized and sealed on-site and transported on ice to the laboratory immediately, where the samples were stored at −20 °C prior to chemical analysis (Smyth et al. 2007). All containers in contact with samples were previously washed with deionized water, acetone, dichloromethane, and the river sample in the stated sequence.
Analytical methods
Water samples were extracted using C18 columns. Sediments were extracted with a Soxhlet extraction system. GC–MS analysis was carried out with a Hewlett–Packard (HP) 6890GC/5973MS system operating in the selected ion monitoring (SIM) mode. Detailed extraction and analytical information is presented in the supplementary materials.
Quality control and assurance
The surrogate standard for the water and sediment samples was d3-AHTN, while HMB was used as the internal standard to quantify the musk concentrations in samples. The sample concentrations were corrected with the recovery of surrogate standards. The blanks, recoveries, and deviations were within acceptable ranges, and detailed materials are listed in the supplementary materials. The limit of detection (LOD) and limit of quantification (LOQ) were determined from a signal to noise ratio of 3 and 6, respectively. In the Songhua River, LOQs of water samples were 2 ng/L for HHCB, MX, and MK and 1 ng/L for AHTN, ADBI, and AHMI. LOQs of sediment samples were 0.5 ng/g for HHCB, AHTN, MX, and MK and 0.2 ng/g for ADBI and AHMI, respectively.
Environmental risk assessment
Environmental risk assessment was carried out based on an exposure concentration to predicted no-effect concentration (PNEC) ratio, namely the risk characterization ratio (RCR). If the RCR is ≥1, a high risk is expected, while RCR ≤0.01 indicates no environmental risk (Balk and Ford 1999b).
Results and discussion
SM levels in water and sediment phases of the Songhua River
The SM concentration ranges and detection frequencies in water and sediment of the Songhua River are listed in Table 1. The concentration details are listed in Tables S1, S2, S3, and S4 in the supplementary materials.
SM levels varied widely in both matrices depending on the chemical compound considered, sampling location, and time of sampling. The total concentrations of six target compounds at different sampling times are listed in Fig. S1 in the supplementary materials, where the concentration levels in January 2012 and April 2012 distinctly dominated. HHCB and AHTN were detected in the majority of water and sediment samples. ADBI, AHMI, and MK were also detected in several samples. However, MX was not detected in all the water and sediment samples. The concentrations of HHCB and AHTN clearly dominated, which was consistent with the results detected in STP effluents (Salgado et al. 2010; Lu et al. 2014), and therefore, this study and the following discussion focused on these two compounds.
Table S5 compares this work and previous works. In general, SM contamination levels in the Songhua River were in the normal ranges found in previous works. Concentrations of HHCB and AHTN in water were similar to those reported in the Hudson River, USA (HHCB 3.95–25.80 ng/L; AHTN 5.09–22.80 ng/L) (Reiner and Kannan 2011), Haihe River, China (HHCB 3.5–32.0 ng/L; AHTN 2.3–26.7 ng/L) (Hu et al. 2011), Tamar estuarine, England (HHCB 6.0–28.0 ng/L; AHTN 3.0–10.0 ng/L) (Sumner et al. 2010), and Lake Zürich, Switzerland (HHCB <2–47 ng/L; AHTN <1–18 ng/L) (Buerge et al. 2003). HHCB and AHTN levels in sediment were similar to those measured in the Haihe River, China (HHCB 1.5–32.3 ng/g dry weight (dw); AHTN 2.0–21.9 ng/g dw) (Hu et al. 2011), and Lake Erie and Lake Ontario, USA (HHCB 3.2–16.0 ng/g dw; AHTN 0.96 ng/g dw) (Peck et al. 2006).
Seasonal variations in HHCB and AHTN levels in water and sediment of the Songhua River
Seasonal variation in HHCB and AHTN levels at different sampling sites had the same tendencies (see Tables S1, S2, S3, and S4). Therefore, the data at sampling sites S5 and S8 were selected for further analysis because the SM levels were comparatively higher and detailed environmental parameters were known. As shown in Fig. 2, concentrations collected from sites S5 and S8 tended to be higher in cold months than in warm months. This phenomenon has been seen recently in four small freshwater river systems in Hessen, Germany, although there have also been conflicting results for seasonal variation of SMs in other studies (Quednow and Püttmann 2008).
Due to stable inputs (Dsikowitzky et al. 2002; Salgado et al. 2011), SM levels in rivers are primarily determined by temperature, illumination, and runoff, which can affect the rate and pattern of sedimentation, volatilization, degradation, photolysis, and dilution (Heberer 2002; Peck et al. 2006; Wang et al. 2012, 2013). Relationships between SM concentrations (HHCB and AHTN) and environmental variables (monthly average temperatures, illumination, and runoff) at sampling sites S5 and S8 are presented in Fig. S2. These temperatures and SM levels have a slightly negative correlation (Fig. S2 [a2] and [a3]). Similarly, illumination and SM levels also have a slightly negative correlation (Fig. S2 [b2] and [b3]). River runoff and SM concentration have a clear negative correlation (Fig. S2 [c2] and [c3]), which indicates that flow velocity has an important impact on both distribution and variance of SM concentrations (Fono et al. 2006; Reiner and Kannan 2011; Chase et al. 2012; Wang et al. 2012). For instance, at site S5, the STP discharge was 3.36 to 3.70 m3/s (Lu et al. 2014), while the Songhua River runoff (299.01 to 2368.74 m3/s) was much greater. Clearly, the most important factor controlling SM concentration was the volume of river runoff, which diluted the pollutants (Wang et al. 2012; Lu et al. 2014).
As shown in Fig. S2 (c1), the overall flow of the Songhua River occurs in three distinct hydrological seasons: a 6-month low-flow period (from November to April), moderate-flow periods (May, June, and October), and a high-flow period (from July to September) (Wang et al. 2012). In addition, runoff in the cold season was just 1/6 to 1/16 of that in the warm season. For example, in April 2012, the smallest runoff volume corresponded to the highest SM concentrations. In contrast, the largest runoff occurred with the lowest concentrations. In addition, SM concentrations were found to be higher in January and April, due to decreasing river flow, low temperatures, and low illumination in the low-flow period compared to other periods. The winter ice-bound period of almost 5 months greatly aggravated the SM levels, partly because low flow and low temperatures hampered dilution and degradation in reducing pollutant concentration and partly because fugacity tends to rise inversely proportional to environmental temperature and enhances the capacity of “holding” pollutants in the aquatic environment as temperatures decrease. Finally, the river went through a thawing phase in April 2012, with SM concentrations reaching a maximum after the low-flow period (see Table S3).
Correlations of normalized concentrations and normalized runoffs for HHCB and AHTN in water and sediment at sites S5 and S8 are shown in Fig. 3 and can be determined by the following equation:
where C t is the predictive concentration of HHCB or AHTN in different sampling periods at one given sampling site (ng/L or ng/g dw); C t,max is the maximum concentration in 1 year at this site (ng/L or ng/g dw); R is the monthly average runoff at this site (m3/s); R max is the minimum monthly average runoff corresponding to C t,max; and A, B, and K t are three fitting parameters. For eight sets of data shown in Fig. 3, the fitting parameters and R 2 can be found in Table S6. From the R 2 and the fitting curves in Fig. 3, we found that the nonlinear regressions were satisfactory. Using Eq. (1), we can easily estimate the SM concentrations in different months at one location.
From Fig. 3, we can also see that normalized SM concentrations in water are closer to 0 than those in sediment in the high runoff periods, which indicates that SM levels in water are more susceptible to the impact of runoff.
Spatial profiles of HHCB and AHTN in water and sediment of the Songhua River
Spatial distributions of HHCB and AHTN detected in water and sediment phases are shown in Fig. 4, which shows that HHCB and AHTN have the same spatial profile. The spatial variation of HHCB concentrations was consistent with that of AHTN (Fig. 4). Similarly, Fig. 5 shows that there was a solid positive correlation between concentrations of HHCB and AHTN in water and sediment.
As presented in Fig. 4, sampling sites along the Songhua River were polluted to a certain extent separately from the river’s source (sampling sites S1 and AS13). Higher concentrations of HHCB and AHTN were detected at S5 (the maximum), S8, AS5, S2, and AS17, which were all located downstream of cities with dense populations (Fig. 4) and large wastewater discharges (Dsikowitzky et al. 2002; Fono et al. 2006; Chase et al. 2012). Lower levels of SMs in water and sediment were found at S1, AS13, AS1, AS14, and S7, likely owing to the sparse population around the sites (Fig. 4) along with dilution effects from adjoining branches of the river (Buerge et al. 2003; Wang et al. 2010). The concentrations of HHCB and AHTN in surface water and sediment show an obvious spatial distribution feature: concentrations downstream of cities were distinctly higher than those found upstream (Dsikowitzky et al. 2002; Bester 2005). Furthermore, the levels of SM concentrations in water and sediment depended on the distance of the sampling sites to the pollution source (Fig. 4) (Dsikowitzky et al. 2002; Yoon et al. 2010; Gómez et al. 2012).
The pollution levels in the Songhua River are related to population density (Dsikowitzky et al. 2002; Wang et al. 2010). As presented in Fig. 4, the greater the population density, the higher the contamination levels. Figure S3 presents positive correlations between concentrations of HHCB and AHTN and population densities around sampling sites in water and sediment in April 2012. Similar relationships can be found in other monitored seasons. Experimental data reported in Fig. S3 can be fitted with the following equations:
where C s is the predictive concentration of HHCB or AHTN (ng/L or ng/g dw), C s,max is the maximum value of C s , D is the population density around the sampling site (number of persons/km2), and D 0,max is the maximum no-effect population density that can be determined by survey or regress. K s is a fitting parameter. For four sets of data shown in Fig. S3, the fitting parameters are provided in Table S7. The coefficients of determination (R 2) are 0.8492 and 0.8221 to HHCB and AHTN for water and 0.6311 and 0.7162 for sediment, respectively. Through Eq. (2), we can estimate the maximum SM concentrations in different locations along the Songhua River.
Combining Eqs. (1) and (2), we can preliminarily predict the SM levels in the entire mainstream of the Songhua River at any time and place. Furthermore, the overall environmental risk assessments for aquatic organisms can be performed based on these predicted SM levels. Nevertheless, in order to provide a more realistic forecast and assessment, a dynamic multimedia fate model of SMs in the Songhua River is needed (Wang et al. 2012).
HHCB/AHTN ratios in water and sediment of the Songhua River
The ratios of the concentrations of HHCB and AHTN in different aquatic compartments have been used as a tool to characterize differences in partitioning (Dsikowitzky et al. 2002). As illustrated in Fig. S4, HHCB/AHTN ratios ranged from 1.1 to 4.3 (mean = 3.1) and 1.7 to 3.1 (mean = 2.3) in water and sediment, respectively, which are in close agreement with results from studies in other regions (HHCB/AHTN = 2.9–5.8) (Buerge et al. 2003; Peck and Hornbuckle 2004). The mean HHCB/AHTN ratios in water are higher than those in sediment mainly as a result of the higher photolysis rate of AHTN relative to that of HHCB (Buerge et al. 2003; Peck et al. 2006) and the degradation of HHCB to HHCB-lactone in sediment (Bester 2005). Variations of HHCB/AHTN ratios are possibly due to variations in ongoing discharge and SM degradation along the river (Lu et al. 2011).
Figure 5 presents the significant positive correlation between HHCB and AHTN concentrations in water and sediment samples (R 2 = 0.9039, 0.9584, p < 0.05), suggesting the coexposure of HHCB and AHTN in the Songhua River Basin (Wang et al. 2010). Moreover, the positive correlation between HHCB/AHTN ratios and SM concentrations suggests that the HHCB/AHTN ratio could be used as a tracer for source discrimination and for the degree of degradation in the environment (Buerge et al. 2003; Zeng et al. 2008). Positive correlations were also found between concentrations of HHCB and other SMs (ADBI, AHMI, and MK, p < 0.05, see Table S1, S2, S3 and S4), which indicate potential feasibility to predict levels of other SMs based on levels of HHCB (Lee et al. 2010).
Linear regression analysis was performed between the concentrations of HHCB and AHTN in water and sediment samples with Eqs. (4) and (5) as follows:
where C wAHTN and C sAHTN are the predictive concentrations of AHTN in water (ng/L) and sediment (ng/g dw) and C wHHCB and C sHHCB are the measured concentrations of HHCB in water (ng/L) and sediment (ng/g dw).
Partitioning of SMs between dissolved and sedimentary phases
The partition characteristics in water and sediment for selected SMs in the Songhua River were determined. A positive correlation between concentrations in water and those in sediment was observed for HHCB and AHTN, and the relationships were determined using Eqs. (6) and (7):
The R 2s are 0.7717 and 0.6803. According to the above relationships, SM levels in sediment can be roughly estimated based on those in water (Lee et al. 2010).
Results also indicated that HHCB and AHTN have similar tendencies to partition between aqueous and solid matrices, which can be explained by the similar log K oc values of 4.65 and 4.74, respectively. Previous studies have indicated that sediment is the final destination of SMs and the overlying water is a significant factor affecting SM concentrations in sediment (Hu et al. 2011; Reiner and Kannan 2011), which can explain the positive correlations between concentrations in sediment and water (Wang et al. 2010).
Environmental risk assessment to aquatic organisms
This study found that HHCB and AHTN are the primary musk contaminants found in the Songhua River, with both SMs being present at levels of nanogram per liter. The PNECs of HHCB and AHTN for aquatic organisms were estimated to be 6.8 and 3.5 μg/L (Balk and Ford 1999b). All RCRs calculated based on this data were below 0.01 as shown in Fig. S5, suggesting that these substances will not pose an environmental risk in the near future (Balk and Ford 1999b).
The spatial distribution maps for the environmental risk from HHCB and AHTN in four seasons are shown in Fig. S6. From the spatial distribution patterns, there appears to be a high degree of spatial variability in the SM RCRs. One hot-spot area was observed at S5, downstream of Harbin city, which is the most developed region along the Songhua River, with a population of about 4.65 million. Here, the river mainstream receives the greatest pollution from both point (STP effluent) and nonpoint (directly draining urban domestic sewage) sources (Wang et al. 2013). Moreover, due to the higher RCR value in the cold season, it is critical to select effective STP treatment processes for SM elimination under conditions of extreme cold.
Conclusion
SMs are widespread in water and sediment of the Songhua River. HHCB and AHTN were found to be the principal pollutants. Temporal profiles were found to be seasonal, which is mainly attributed to seasonal changes of environmental variables. Higher contamination in the cold season is most likely caused by the following: (1) low dilution, (2) low microbial degradation rate of SMs, (3) low volatilization and photolysis of SMs prevented by ice and snow layers, (4) low levels of dissolved oxygen below the ice layer that reduces SM degradation, (5) relatively large STP emission of SMs, most likely caused by the relatively lower STP removal efficiency at low temperatures particularly for biological oxidation processes (Lu et al. 2014).
Spatial differences of SM levels in the Songhua River mainly were found in total SM emission rates, related to population densities around the sampling sites. SM concentrations downstream of cities were distinctly higher than those found upstream. Peak concentrations were measured downstream of Harbin city (site S5), which has a high population density and considerable volume of direct drainage of urban domestic sewage into the river. Environmental risk assessment to aquatic organisms was performed, and all RCRs were calculated to be below 0.01, indicating that SMs are unlikely to pose an environmental risk in the near future.
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Acknowledgments
This work was supported by the National Major Science and Technology Projects for Water Pollution Control and Governance (2009ZX07207-008-5-2), the National Creative Research Groups of China (51121062), and the National Science Fund for Distinguished Young Scholars (51125033). We wish to thank the Harbin Station of Environmental Monitoring, Heilongjiang Province, China, for river runoff data and sampling of water and sediment.
Conflict of interest
All authors have disclosed any actual or potential conflict of interest including any financial, personal, or other relationships with other people or organizations within 3 years of beginning the submitted work that could inappropriately influence, or be perceived to influence, their work.
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Lu, B., Feng, Y., Gao, P. et al. Distribution and fate of synthetic musks in the Songhua River, Northeastern China: influence of environmental variables. Environ Sci Pollut Res 22, 9090–9099 (2015). https://doi.org/10.1007/s11356-014-3973-6
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DOI: https://doi.org/10.1007/s11356-014-3973-6