Abstract
We previously isolated a mutant of Saccharomyces cerevisiae strain 85_9 whose glycerol assimilation was improved through adaptive laboratory evolution. To investigate the mechanism for this improved glycerol assimilation, genome resequencing of the 85_9 strain was performed, and the mutations in the open reading frame of HOG1, SIR3, SSB2, and KGD2 genes were found. Among these, a frameshift mutation in the HOG1 open reading frame was responsible for the improved glycerol assimilation ability of the 85_9 strain. Moreover, the HOG1 gene disruption improved glycerol assimilation. As HOG1 encodes a mitogen-activated protein kinase (MAPK), which is responsible for the signal transduction cascade in response to osmotic stress, namely the high osmolarity glycerol (HOG) pathway, we investigated the effect of the disruption of PBS2 gene encoding MAPK kinase for Hog1 MAPK on glycerol assimilation, revealing that PBS2 disruption can increase glycerol assimilation. These results indicate that loss of function of Hog1 improves glycerol assimilation in S. cerevisiae. However, single disruption of the SSK2, SSK22 and STE11 genes encoding protein kinases responsible for Pbs2 phosphorylation in the HOG pathway did not increase glycerol assimilation, while their triple disruption partially improved glycerol assimilation in S. cerevisiae. In addition, the HOG1 frameshift mutation did not improve glycerol assimilation in the STL1-overexpressing RIM15 disruptant strain, which was previously constructed with high glycerol assimilation ability. Furthermore, the effectiveness of the HOG1 disruptant as a bioproduction host was validated, indicating that the HOG1 CYB2 double disruptant can produce L-lactic acid from glycerol.
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Introduction
The budding yeast Saccharomyces cerevisiae has been widely used not only for brewing alcoholic beverages, such as wine and Japanese rice wine (sake), but also for producing bioethanol and industrially useful materials, including organic acids, alcohols, and intermediate compounds of pharmaceutical drugs. Moreover, it has been used as a eukaryotic model organism for researches in genetics and molecular biology.
In biodiesel production, triacylglycerol obtained from plants and animal oils is converted to fatty acid esters and glycerol by transesterification with alcohols (Parawira 2009). Fatty acid esters can be used as fuels, but there are few appropriate ways to utilize glycerol as a byproduct in biodiesel production. Therefore, efficient strategies for using glycerol are highly desirable. To date, many studies on glycerol utilization as a carbon source for bioproduction using microorganisms have been reported (Asskamp et al. 2019; Jo et al. 2023; Tokuyama et al. 2014; Vikromvarasiri et al. 2021; Zhu et al. 2002).
It is known that S. cerevisiae has metabolic reactions to assimilate glycerol as a carbon source (Klein et al. 2017). However, S. cerevisiae cannot grow well on glycerol as a sole carbon source, and the reason for this phenomenon is unclear (Swinnen et al. 2013). Previously, to obtain S. cerevisiae strains with high glycerol assimilation ability through metabolic engineering, we performed adaptive laboratory evolution of the S. cerevisiae W303-1B strain, in which the serial transfer of a culture to a fresh medium containing glycerol as the main carbon source was repeated until the cell growth rate increased by three times (Kawai et al. 2019). Based on transcriptome analysis of the evolved population exhibiting high growth rate on glycerol, we constructed recombinant strains with high growth ability on glycerol, and we successfully improved glycerol assimilation by overexpressing HAP4 or disrupting RIM15 genes together with overexpressing the STL1 gene. However, the growth rate of the recombinant strains was lower than that of the population obtained by adaptive laboratory evolution. In addition, the metabolic flux distribution of the evolved 85_9 strain, which was isolated by single colony isolation of the evolved population after 85 generations in the adaptive laboratory evolution, showed the fastest growth rate among the strains isolated (Yuzawa et al. 2021). However, the mechanisms for the improved glycerol assimilation in 85_9 strain are still unclear.
Glycerol functions as a compatible solute against increasing osmotic pressure in S. cerevisiae (Blomberg and Adler 1989). When S. cerevisiae responds to an increase in osmotic pressure, a signal transduction cascade, namely high osmolarity glycerol (HOG) pathway, is activated, and the mitogen-activated protein kinase (MAPK) Hog1 is phosphorylated (Brewster et al. 1993). Two branches are present in the HOG pathway; Sln1 and Sho1. In the Sln1 branch, the signal is transferred by a phosphorylation relay from Sln1 on the cytoplasmic membrane through Ypd1 and Ssk1 to MAPK kinase kinases (MAPKKKs) Ssk2 and Ssk22 in the cytoplasm (Posas et al. 1996). In the Sho1 branch, the Sho1 protein on the cytoplasmic membrane recruits the Ste11 MAPKKK via a scaffold protein Pbs2, which is a MAPK kinase (MAPKK) responsible for the phosphorylation of Hog1 (Posas and Saito 1997). Phosphorylated Ssk2, Ssk22, and Ste11 phosphorylate Pbs2 MAPKK, and phosphorylated Pbs2 phosphorylates Hog1 MAPK (Brewster et al. 1993; Posas and Saito 1997; Posas et al. 1996). Phosphorylated Hog1 MAPK is transferred to the nucleus with importin β encoded by NMD5 (Ferrigno et al. 1998) and phosphorylates the stress-responsive transcription factors Msn2 and Msn4 (Rep et al. 2000). Phosphorylated Msn2 and Msn4 induce the transcription of genes related to glycerol synthesis (Rep et al. 2000). However, the relationship between Hog1 and glycerol assimilation remains unknown.
In the present study, we performed genome resequencing analysis of S. cerevisiae 85_9 strain and evolved population to understand the mechanisms of glycerol assimilation, and we identified the mutation responsible for improved glycerol assimilation in that strain. We found that loss of function of Hog1 by its mutation or PBS2 disruption resulted in improved glycerol assimilation in S. cerevisiae. Moreover, the effect of disruption of genes related to the HOG pathway on glycerol assimilation was investigated. In addition, the effectiveness of the HOG1 disruptant as a host for bioproduction from glycerol was demonstrated.
Materials and methods
Strains, plasmids, and media
S. cerevisiae W303-1B (MATα leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15) and its glycerol-assimilating mutant strain 85_9, previously obtained through adaptive laboratory evolution and single-colony isolation (Yuzawa et al. 2021), were used. In addition, the STL1-overexpressing RIM15 disruptant of S. cerevisiae constructed in a previous study (Kawai et al. 2019) was used. For recombinant DNA experiments, Escherichia coli MG1655 (F–, wild-type) and DH5α (F– Φ80dlacZΔM15 Δ(lacZYA-argF) U169 deoR recA1 endA1 hsdR17(rK–, mK+) phoA supE44 λ–thi-1 gyrA96 relA1) were used.
To amplify the gene disruption cassette for S. cerevisiae, the plasmid pUG73 carrying the LEU2 gene from Kluyveromyces lactis (KlLEU2) (Gueldener et al. 2002) was used. To remove the selection marker in the disruption cassette from the genome of gene disruptants in S. cerevisiae, plasmid pSHAUR1 (Ida et al. 2012) was used. To clone the mutant KGD2 gene from the 85_9 strain and the gene encoding L-lactate dehydrogenase (LDH) from Xenopus laevis, a T-vector pMD20 (Takara Bio Inc., Shiga, Japan) and an expression vector for S. cerevisiae pGK426 (Ishii et al. 2009), provided by the National Bio-Resource Project, Japan, were used. In addition, pGK425-STL1, which was constructed in a previous study (Kawai et al. 2019), was used to overexpress the STL1 gene in S. cerevisiae.
To construct recombinant strains of S. cerevisiae, YPAD medium [10 g L–1 Bacto yeast extract (Difco Laboratories, Detroit, MI), 20 g L–1 Bacto peptone (Difco Laboratories), 20 g L–1 glucose, and 0.2 g L–1 adenine hemisulfate], 2× YPAD medium (20 g L–1 Bacto yeast extract, 40 g L–1 Bacto peptone, 40 g L–1 glucose, 0.4 g L–1 adenine hemisulfate), YPAGal medium (10 g L–1 Bacto yeast extract, 20 g L–1 Bacto peptone, 20 g L–1 galactose, 0.2 g L–1 adenine hemisulfate), and SC medium [6.7 g L–1 yeast nitrogen base without amino acids (Difco Laboratories), 20 g L–1 glucose, 0.2 g L–1 adenine hemisulfate, 0.076 g L–1 L-tryptophan, 0.076 g L–1 uracil, 0.076 g L–1 L-leucine, 0.076 g L–1 L-histidine-HCl, 1.4 g L–1 yeast synthetic drop-out media supplements without histidine, leucine, tryptophan, and uracil (Sigma-Aldrich, Inc., St. Louis, MO)] were used. To prepare agar plates, 20 g L–1 agar was added to the medium. To analyze glycerol assimilation in S. cerevisiae, MD medium (6.7 g L–1 yeast nitrogen base without amino acid, 20 g L–1 glucose, 0.2 g L–1 adenine hemisulfate, 0.076 g L–1 L-histidine-HCl, 0.076 g L–1 L-tryptophan, 0.076 g L–1 L-leucine, 0.076 g L–1 uracil) and MG medium, in which 20 g L–1 glycerol was added instead of glucose, were used. For the assay of L-lactate production, modified MG medium containing 6.7 g L–1 yeast nitrogen base without amino acid, 20 g L–1 glycerol, 0.2 g L–1 adenine hemisulfate, 0.38 g L–1 L-histidine-HCl, 0.38 g L–1 L-tryptophan, 0.38 g L–1 L-leucine was used. If necessary, uracil and L-leucine were removed from the culture medium.
For plasmid construction, E. coli was cultured in Lennox (L) medium containing 10 g L–1 hipolypeptone (Nihon Pharmaceutical Co., Ltd., Tokyo, Japan), 5 g L–1 dried yeast extract D-3 H (Nihon Pharmaceutical Co.), 5 g L–1 NaCl, and 1 g L–1 glucose (pH 7.0). To prepare the L agar plates, 15 g L–1 agar was added to the medium. If necessary, 50 mg L–1 ampicillin was added to the L medium.
Genome resequencing analysis
For genome resequencing analysis, genomic DNA was isolated from S. cerevisiae using Gentra Puregene Yeast/Bact. kit (Qiagen, Venlo, The Netherlands). Genome resequencing analysis of the S. cerevisiae W303-1B and 85_9 strains, as well as the cell population obtained from the culture after 85 generations in the adaptive laboratory evolution, was performed using the MiSeq system (Illumina, Inc., San Diego, CA). Mutations in the 85_9 strain and cell population after 85 generations of adaptive laboratory evolution were identified using the Mudi web tool (https://naoii.nig.ac.jp/mudi_top.html) (Iida et al. 2014).
The fastq files obtained from genome resequencing analysis are available under accession numbers DRA452749, DRA452750, and DRA452751.
Introduction of mutations identified in the 85_9 mutant into the W303-1B strain
It was reported that the expression of E. coli mazF gene, which encodes mRNA interferase, is toxic to S. cerevisiae (Liu et al. 2014). Based on this report, we developed a system to introduce mutations into S. cerevisiae. Briefly, the mazF gene with the S. cerevisiae GAL1 promoter was first introduced into the target gene site of S. cerevisiae grown in a medium containing glucose to prevent expression of the mazF gene. The resulting strain was transformed again with the DNA fragment carrying a mutation, and the transformants were selected on a medium containing galactose. If the mazF gene in the target site is replaced with the introduced DNA fragment carrying a mutation, cells can grow on galactose.
First, a plasmid containing KlLEU2 and E. coli mazF was constructed. E. coli mazF gene was amplified through polymerase chain reaction (PCR) from the genomic DNA of E. coli MG1655 using KOD-Plus-Neo (Toyobo Co., Ltd., Osaka, Japan) and the primer set shown in Table S1. In addition, the GAL1 promoter and CYC1 terminator were amplified from the genomic DNA of S. cerevisiae W303-1B through PCR using the primer sets shown in Table S1. These three fragments were connected using overlap extension PCR, and the connected fragment was inserted into the SacI site of pUG73. The resulting plasmid was designated pUG73-mazF.
Next, the DNA fragments containing the KlLEU2 and mazF genes with upstream and downstream flanking sequences of HOG1, SIR3, and SSB2 genes were amplified through PCR from the pUG73-mazF using primer sets shown in Table S1. Each amplified fragment was introduced into the W303-1B strain using the lithium acetate method reported by Gietz and Woods (2002) and L-leucine-prototrophic transformants were obtained on SC medium. Then, the DNA fragment was amplified from the genomic DNA of the 85_9 strain through PCR using the primer sets shown in Table S1, introduced into the corresponding L-leucine-prototrophic transformant, and transformants were selected on YPAGal medium. The introduction of mutations was confirmed by sequencing of the DNA fragment amplified through PCR from the genome of the transformant selected on YPAGal medium using the primer sets shown in Table S1.
As the strain carrying the KGD2 gene mutation found in the 85_9 strain could not be obtained using the methods described above, the KGD2-disrupted strain harboring a plasmid with the mutant KGD2 gene was constructed. The mutant KGD2 gene was amplified from the genomic DNA of the 85_9 strain, through PCR using the primer set shown in Table S1. The amplified fragment was treated with 10×A attachment mix (Toyobo Co.) and cloned into the T-vector pMD20. After confirming the nucleotide sequence of the cloned fragment, it was subcloned into the SalI-EcoRI sites of pGK426 (Ishii et al. 2009). The resulting plasmid was introduced into the KGD2-disrupted strain. The methods for constructing the KGD2-disrupted strain are described in the following section.
Gene disruption in S. cerevisiae
To disrupt genes in S. cerevisiae, a disruption cassette (loxP-KlLEU2-loxP) with upstream and downstream flanking regions for each target gene was amplified through PCR from the template plasmid pUG73 (Gueldener et al. 2002) using KOD-Plus-Neo and KOD One (Toyobo Co.) and primer sets listed in Table S1. The disruption cassettes were introduced into the W303-1B strain, and the disruption of the target genes was checked through PCR using the primer sets shown in Table S1. To disrupt multiple genes, KlLEU2 sequence at the disrupted site was removed from the genome by introducing plasmid pSHAUR1 (Ida et al. 2012) into the constructed disruptants and expressing Cre recombinase in YPGal medium to induce homologous recombination between loxP sites in the disruption cassette, and then a disruption cassette for another gene was introduced.
Construction of a plasmid carrying the L-lactate dehydrogenase gene from X. laevis
To construct a plasmid carrying the gene for LDH from X. laevis, a gene fragment, whose codons were optimized for S. cerevisiae, was synthesized (GeneArt Strings DNA Fragments; Thermo Fischer Scientific, Waltham, MA) (Fig. S1). The fragment was amplified through PCR from this synthesized fragment using the primer set shown in Table S1 and then cloned into pMD20. After confirming the nucleotide sequence of the cloned fragment, it was subcloned into the SalI-BamHI sites of pGK426 (Ishii et al. 2009), and the resulting plasmid was designated pGK426-Xe_LDH. Finally, the pGK426-Xe_LDH plasmid was introduced into HOG1 single and HOG1 and CYB2 double disruptants of W303-1B.
Analysis of the glycerol assimilation ability
The glycerol assimilation ability of S. cerevisiae was analyzed by monitoring cell growth on glycerol. The preculture was prepared by culturing S. cerevisiae in the MD medium. For the main culture, cells from the preculture washed with MG medium were diluted to an optical density at 660 nm (OD660) of 0.05, and then cultured at 30 °C for 168 h. The cells were cultured in 5 mL of MG medium with reciprocal shaking at 150 strokes/min using test tubes. The OD660 was measured using a spectrophotometer UV-1280 (Shimadzu Co., Kyoto, Japan).
L-Lactic acid production assay
Cells of the HOG1 single and HOG1 and CYB2 double disruptants carrying pGK426-Xe_LDH were cultured in 300-mL Erlenmeyer flasks containing 50 mL of modified MG medium with rotary shaking at 200 rpm to prevent sedimentation of the cells. Time courses of OD660 and the concentrations of glycerol and L-lactic acid in the culture supernatant were measured. Concentrations of L-lactic acid, ethanol, and glycerol in the culture supernatant were measured using F-kit L-lactate (Roche Diagnostics GmbH, Mannheim, Germany), F-kit ethanol (Roche Diagnostics GmbH), and Glycerol GK assay kit (Megazyme Ltd., Bray, Ireland), respectively.
Results
Genome resequencing of the 85_9 mutant strain and identification of the mutation responsible for glycerol assimilation
We previously performed adaptive laboratory evolution of S. cerevisiae W303-1B strain to achieve a high growth rate in glycerol as the main carbon source, and the specific growth rate increased by approximately 3-fold after 85 generations (Kawai et al. 2019). The mutant strain 85_9 showed the highest specific growth rate among the strains randomly isolated from the evolved population after 85 generations (Yuzawa et al. 2021). To understand the high glycerol assimilation phenotype of the 85_9 strain, we analyzed the genome sequence of the strain and the evolved population after 85 generations, compared with that of the W303-1B parental strain. We identified mutations in the open reading frame (ORF) of the 85_9 strain, which overlapped in the evolved population after 85 generations. We found that four genes, HOG1, SIR3, SSB2, and KGD2 had a mutation in their ORFs; a frameshift mutation was found in the ORF of HOG1, while missense mutations were found in the ORF of the other genes (Table 1). These mutations in the 85_9 genome were confirmed using Sanger sequencing.
Next, we determined which mutation(s) caused the high glycerol assimilation ability of the 85_9 strain by introducing each identified mutation into the genome of the parental W303-1B strain and observing cell growth of the mutation-introduced strains on glycerol. Although the mutations in HOG1, SIR3, and SSB2 genes were successfully introduced into the genome of the W303-1B strain, the mutation in the KGD2 gene was not. Therefore, the mutant KGD2 gene cloned into the plasmid was introduced into the KGD2-disrupted W303-1B strain and its growth in glycerol was analyzed. As shown in Fig. 1a, only the strain with a frameshift mutation in the HOG1 gene grew on glycerol, indicating that the high glycerol assimilation phenotype in the 85_9 strain is caused by a frameshift mutation in the HOG1 gene.
To investigate whether only the HOG1 frameshift mutation caused high glycerol assimilation, we compared growth in glycerol of the W303-1B strain carrying the HOG1 frameshift mutation with that of the 85_9 strain. As shown in Fig. 1b, growth on glycerol was significantly higher in the 85_9 strain than that in the W303-1B strain with a HOG1 frameshift mutation. This result indicates that other unknown factor(s), in addition to the HOG1 frameshift mutation, caused high glycerol assimilation in the 85_9 strain.
Furthermore, to determine when these four mutations were introduced through adaptive laboratory evolution, nucleotide sequences of these four genes in the populations obtained from the culture after 3 and 35 generations of adaptive laboratory evolution (Kawai et al. 2019) were analyzed. The HOG1 frameshift mutation was found in the cells from the populations at 3 and 35 generations, while the other mutations were detected in the cells from the evolved population at 85 generations (data not shown). These results indicate that the HOG1 frameshift mutation introduced in the first three generations causes an initial increase in cell growth during the adaptive laboratory evolution (Kawai et al. 2019).
Effect of disruption of genes related to the HOG pathway on glycerol assimilation
Hog1 is a MAPK and is involved in signal transduction in response to osmotic stress via the HOG pathway (Brewster et al. 1993). As the HOG1 gene mutation in the 85_9 strain was a frameshift mutation (Table 1), we investigated the effect of HOG1 gene disruption on glycerol assimilation in S. cerevisiae W303-1B. As shown in Fig. 1c, disruption of the HOG1 gene significantly increased glycerol assimilation in S. cerevisiae.
In the HOG pathway, Hog1 is phosphorylated by Pbs2 MAPKK, and phosphorylated Hog1 phosphorylates its target proteins in the nucleus, including transcription factors and other proteins, to facilitate downstream cellular functions (Brewster et al. 1993). Therefore, we investigated the effect of PBS2 gene disruption on glycerol assimilation in S. cerevisiae and found that the disruption improved cell growth on glycerol (Fig. 2a). These results indicate that loss of function of Hog1 MAPK caused by disruption of HOG1 and PBS2 genes improves glycerol assimilation in S. cerevisiae.
As described in the Introduction, two individual signal transduction pathways lead to Pbs2 phosphorylation: Ssk2 and Ssk22 MAPKKKs phosphorylate the Pbs2 MAPKK receiving signals from Sln1 protein on the cytoplasmic membrane through Ypd1 and Ssk1, while Ste11 MAPKKK phosphorylates the Pbs2 responding signals by Sho1 protein on the cytoplasmic membrane (Posas and Saito 1997; Posas et al. 1996). Therefore, the effect of disrupting the genes encoding MAPKKKs, which are responsible for Pbs2 phosphorylation on glycerol assimilation, was examined. As shown in Fig. 2b, S. cerevisiae did not grow well on glycerol as the main carbon source by disruption of either STE11, SSK2, or SSK22 genes, while their triple disruption partially improved growth on glycerol. In addition, triple disruption of SSK2, SSK22, and SHO1 did not improve glycerol assimilation (Fig. 2c). These results indicate that inactivation of MAPKKKs in the HOG pathway toward Pbs2 phosphorylation results in partial improvement of glycerol assimilation in S. cerevisiae.
Effect of RIM15 disruption with STL1 overexpression on glycerol assimilation in the HOG1 frameshift mutant
We previously reported that disruption of the RIM15 gene encoding a Greatwall protein kinase, together with overexpression of the STL1 gene encoding a glycerol importer, resulted in increased glycerol assimilation in S. cerevisiae (Kawai et al. 2019). Therefore, we examined whether glycerol assimilation in the HOG1 frameshift mutant was improved by disrupting RIM15 gene and introducing the STL1 overexpression plasmid pGK425-STL1. As shown in Fig. 3, cell growth of the HOG1 frameshift mutant was improved by RIM15 disruption and STL1 overexpression, but the growth was similar to that of the RIM15 disruptant overexpressing STL1. This result indicates that the synergy of the HOG1 frameshift mutation with RIM15 disruption and STL1 overexpression in glycerol assimilation does not occur.
Utilization of the HOG1 disruptant as a host for L-lactic acid production
Finally, utilization of the HOG1 disruptant as a host in the production of valuable materials was attempted. We constructed a recombinant strain of the HOG1 disruptant to produce L-lactic acid and investigated its L-lactic acid production (Fig. 4). Briefly, the codon-optimized gene encoding LDH from X. laevis was cloned under the PGK1 promoter on the pGK426 vector and introduced into the HOG1 disruptant.
Before conducting the L-lactic acid production assay, we added uracil, L-leucine, L-histidine, and L-tryptophan, which are required for both strains to grow, to the culture of the S. cerevisiae W303-1B strain and its HOG1 disruptant, and examined the effect on the growth and glycerol assimilation. The final nutrient concentration was two and five times higher (0.15 and 0.38 g L–1, respectively) compared with that in the original MG medium (0.076 g L–1). As shown in Figs. S2a and S2b, the W303-1B strain did not grow on glycerol or assimilate glycerol, even though uracil, L-leucine, L-histidine, and L-tryptophan were supplemented. In contrast, in the HOG1 disruptant, when changing concentration of uracil, L-leucine, L-histidine and L-tryptophan in the MG medium, final OD660 value reached at 168 h in the presence of 0.38 g L–1 of uracil, L-leucine, L-histidine and L-tryptophan was larger than that in the presence of 0.076 g L–1 of them and this difference was significant (p < 0.05, t-test) (Fig. S2c). Moreover, concentration of glycerol remained in the presence of 0.38 g L–1 of uracil, L-leucine, L-histidine and L-tryptophan was lower than that in the presence of 0.076 g L–1 of them and this difference was also significant (p < 0.05, t-test)(Fig. S2d). These indicate that glycerol assimilation is improved by additional nutrient supplementation. Therefore, we used modified MG medium, where the concentration of L-leucine, L-histidine, and L-tryptophan was five times higher compared to that in the original MG medium, but uracil was not added because the strain harboring pGK426-Xe_LDH1, which carries the URA3 gene as a selection marker, was cultured in this study.
L-Lactic acid production by the HOG1 disruptant harboring LDH from X. laevis was examined under microaerobic conditions. As shown in Fig. 4a and b, although glycerol was consumed in the HOG1 disruptant harboring X. laevis LDH, L-lactic acid production was not observed in this strain. Moreover, glycerol consumption stopped after consumption of approximately 8 g L–1 for 192 h. As the reason for the lack of L-lactic acid production might be the immediate incorporation of produced L-lactic acid into the cells of the recombinant strain, the reduction of the incorporation of L-lactic acid was attempted. According to Ookubo et al. (2008), disruption of the CYB2 gene encoding L-lactate cytochrome c oxidoreductase, responsible for the reduction of L-lactic acid to pyruvic acid, prevents incorporation of L-lactic acid into the cells due to the decrease in pH in the culture. Therefore, the CYB2 gene was additionally disrupted in the HOG1 disruptant harboring LDH from X. laevis and L-lactic acid production was examined. As shown in Fig. 4c and Table S2, specific growth rate was not changed by the additional CYB2 disruption, but specific glycerol consumption rate was significantly increased (p < 0.05, t-test). Moreover, as shown in Fig. 4d, L-lactic acid production was observed in the LDH-harboring HOG1 disruptant by CYB2 gene disruption and the production levels reached approximately 1.8 g L–1 at 336 h. Similar to the case of the LDH-harboring HOG1 disruptant, glycerol consumption stopped after consuming approximately 10 g L–1 for 144 h. Ethanol was not produced by any of the recombinant strains (data not shown). In addition, the CYB2 disruptant harboring the LDH from X. laevis could not grow on glycerol (data not shown), revealing that the CYB2 disruption does not affect glycerol assimilation in S. cerevisiae. These results indicate that the HOG1 disruptant of S. cerevisiae can be used for bioproduction from glycerol, but it is necessary to solve the problems regarding stopping glycerol consumption to further increase the production of target materials.
Discussion
In this study, to investigate the mechanism for improved glycerol assimilation in the S. cerevisiae 85_9 strain obtained through adaptive laboratory evolution, we identified a mutation that is related to this phenomenon. We found that the frameshift mutation in the HOG1 gene causes the increased glycerol assimilation in the 85_9 strain (Fig. 1a and b). However, the growth rate of the HOG1 frameshift mutant and HOG1 disruptant was lower than that of the 85_9 strain obtained through adaptive laboratory evolution (Fig. 1b and c), indicating that other factor(s), which is responsible for the high glycerol assimilation phenotype in the 85_9 strain, may be present in addition to the HOG1 frameshift mutation. In the present study, the mutations in the open reading frame of the genes were focused. However, some mutations in the intergenic regions of the open reading frames should be found as well. Such mutations might be related to improved glycerol assimilation in the 85_9 strain and introduction of the mutations into the HOG1 frameshift mutant would further improve glycerol assimilation. In addition, 13C-metabolic flux analysis of the 85_9 strain revealed that the increase in the flux to the pentose phosphate pathway might be related to improved glycerol assimilation (Yuzawa et al. 2021). Therefore, genetic modification in the HOG1 frameshift mutant to increase the flux to the pentose phosphate pathway might also be required for further improvement of glycerol assimilation.
In this study, disruption of the HOG1 disruption improved glycerol assimilation in S. cerevisiae, but the growth characteristics of the HOG1 disruptant seemed to be unstable. The specific growth rates of the HOG1 disruptant during exponential phase calculated using the data in Figs. 1c and 2a, b and c were similar (0.036 ± 0.000 h–1, 0.035 ± 0.001 h–1, 0.040 ± 0.002 h–1, and 0.037 ± 0.000 h–1, respectively), but the lag time of growth shown in Fig. 1c was longer than that shown in Fig. 2. This indicates that there might be less reproducibility of the length of lag time, which corresponds to initial adaptation of the HOG1 disruptant to the environment where the strain can grow on glycerol as a carbon source. Once the HOG1 disruptant starts growing after initial adaptation to the condition where it can grow on glycerol, it can exponentially grow well. In addition, rapid adaptation to such environment (i.e. reduction of the lag time) would be required for improving glycerol assimilation in the HOG1 disruptant.
It was expected that Hog1 MAPK would not be active and glycerol assimilation would increase when the SSK2, SSK22 and STE11 genes encoding upstream MAPKKKs in the HOG pathway were disrupted. However, this disruption partially improved glycerol assimilation (Fig. 2b). The mechanism for this phenomenon is unclear, but Pbs2 MAPKK seems to be active when grown on glycerol as the carbon source, even though the genes encoding upstream MAPKKKs were disrupted. There might be unidentified protein kinase(s) responsible for phosphorylation of the Pbs2 and/or Hog1, in addition to Ssk2, Ssk22 and Ste11. In the triple disruptant of the SSK2, SSK22 and STE11 genes, such unidentified protein kinase might moderately activate the Pbs2 and/or Hog1 by phosphorylation and as a result glycerol assimilation in S. cerevisiae would partially be improved. In addition, triple disruption of the SSK2, SSK22, and SHO1 did not result in improved glycerol assimilation. In the triple disruptant of the SSK2, SSK22, and SHO1, Pbs2 and/or Hog1 might completely be phosphorylated by unidentified protein kinase(s) and as a result, glycerol assimilation would not be improved.
Strucko et al. (2018) reported that mutations in the HOG1 and PBS2 genes were found in the evolved populations obtained through adaptive laboratory evolution of S. cerevisiae grown on glycerol as the main carbon source. These researchers suggested that strains carrying a mutation in GUT1 encoding glycerol kinase with that in HOG1 or PBS2 show significant growth in glycerol. Mutations in the HOG1 and PBS2 genes found in the evolved populations resulted in a loss-of-function phenotype, but they did not examine contribution of the mutations in the HOG1 and PBS2 genes to glycerol assimilation in S. cerevisiae. In contrast, we found that only the HOG1 frameshift mutation resulted in significant growth on glycerol. Comparing these studies, it can be concluded that the main cause for significant growth on glycerol is a loss-of-function mutation in the HOG1 gene. In addition, Kvitek and Sherlock (2013) showed that the signaling pathways, including Ras/cAMP/PKA and HOG pathways, are affected by mutations through adaptive laboratory evolution experiments, resulting in loss of sensitivity to environmental changes. These researchers concluded that the loss of signaling networks is a major strategy for adapting to a constant environment. Therefore, S. cerevisiae can grow on glycerol as the carbon source because it does not respond to the environment in which it is grown.
We also demonstrated utilization of the HOG1 disruptant in S. cerevisiae for bioproduction from glycerol. In the present study, the HOG1 disruptant was used as a host for L-lactic acid production from glycerol. However, since L-lactic acid production was not observed in the LDH-harboring the HOG1 disruptant, the CYB2 gene was additionally disrupted in the LDH-expressing HOG1 disruptant because it was assumed that the L-lactic acid produced might be incorporated into the recombinant cells due to the decrease in the pH of the culture (Ookubo et al. 2008). L-Lactic acid production was observed by additional disruption of the CYB2 gene. It should be noted that the specific glycerol consumption rate in the LDH-expressing HOG1 CYB2 double disruptant was higher than that in the LDH-expressing HOG1 disruptant, whereas their specific growth rates were similar (Table S2); difference in specific glycerol consumption rates between two strains was significant (p < 0.05, t-test). When S. cerevisiae cells consume glycerol, the NADH/NAD+ ratio (redox balance) tends to increase (Clomburg and Gonzalez 2013; Merico et al. 2011). Therefore, the increase in the specific glycerol consumption rate might be caused by the increase in NADH oxidation through L-lactic acid production, catalyzed by LDH. Considering these results, it might be useful to determine the compound whose production is related to NADH oxidation as a target for bioproduction from glycerol, using the HOG1 disruptant of S. cerevisiae.
Currently, the mechanism for increased glycerol assimilation by loss of function of the HOG1 gene is still unclear. It is important to understand the mechanism for further improvement of glycerol assimilation toward bioproduction from glycerol. Moreover, utilization of the HOG1 disruptant to produce useful material(s) would be encouraged to demonstrate the effectiveness of the HOG1 disruptant as a bioproduction host.
Data availability
All data generated or analyzed during this study are included in this article and its supplementary information.
References
Asskamp MR, Klein M, Nevoigt E (2019) Saccharomyces cerevisiae exhibiting a modified route for uptake and catabolism of glycerol forms significant amounts of ethanol from this carbon source considered as ‘non-fermentable’. Biotechnol Biofuels 12:257. https://doi.org/10.1186/s13068-019-1597-2
Blomberg A, Adler L (1989) Roles of glycerol and glycerol-3-phosphate dehydrogenase (NAD+) in acquired osmotolerance of Saccharomyces cerevisiae. J Bacteriol 171:1087–1092. https://doi.org/10.1128/jb.171.2.1087-1092.1989
Brewster JL, de Valoir T, Dwyer ND, Winter E, Gustin MC (1993) An osmosensing signal transduction pathway in yeast. Science 259:1760–1763. https://doi.org/10.1126/science.7681220
Clomburg JM, Gonzalez R (2013) Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals. Trends Biotechnol 31:20–28. https://doi.org/10.1016/j.tibtech.2012.10.006
Ferrigno P, Posas F, Koepp D, Saito H, Silver PA (1998) Regulated nucleo/cytoplasmic exchange of HOG1 MAPK requires the importin β homologs NMD5 and XPO1. EMBO J 17:5606–5614. https://doi.org/10.1093/emboj/17.19.5606
Gietz RD, Woods RA (2002) Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol 350:87–96. https://doi.org/10.1016/s0076-6879(02)50957-5
Gueldener U, Heinisch J, Koehler GJ, Voss D, Hegemann JH (2002) A second set of loxP marker cassettes for cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Res 30:e23. https://doi.org/10.1093/nar/30.6.e23
Ida Y, Furusawa C, Hirasawa T, Shimizu H (2012) Stable disruption of ethanol production by deletion of the genes encoding alcohol dehydrogenase isozymes in Saccharomyces cerevisiae. J Biosci Bioeng 113:192–195. https://doi.org/10.1016/j.jbiosc.2011.09.019
Iida N, Yamao F, Nakamura Y, Iida T (2014) Mudi, a web tool for identifying mutations by bioinformatics analysis of whole-genome sequence. Genes Cells 19:517–527. https://doi.org/10.1111/gtc.12151
Ishii J, Izawa K, Matsumura S, Wakamura K, Tanino T, Tanaka T, Ogino C, Fukuda H, Kondo A (2009) A simple and immediate method for simultaneously evaluating expression level and plasmid maintenance in yeast. J Biochem 145:701–708. https://doi.org/10.1093/jb/mvp028
Jo MH, Ju JH, Heo SY, Cho J, Jeong KJ, Kim MS, Kim CH, Oh BR (2023) Production of 1,2-propanediol from glycerol in Klebsiella pneumoniae GEM167 with flux enhancement of the oxidative pathway. Biotechnol Biofuels Bioprod 16:18. https://doi.org/10.1186/s13068-023-02269-4
Kawai K, Kanesaki Y, Yoshikawa H, Hirasawa T (2019) Identification of metabolic engineering targets for improving glycerol assimilation ability of Saccharomyces cerevisiae based on adaptive laboratory evolution and transcriptome analysis. J Biosci Bioeng 128:162–169. https://doi.org/10.1016/j.jbiosc.2019.02.001
Klein M, Swinnen S, Thevelein JM, Nevoigt E (2017) Glycerol metabolism and transport in yeast and fungi: established knowledge and ambiguities. Environ Microbiol 19:878–893. https://doi.org/10.1111/1462-2920.13617
Kvitek DJ, Sherlock G (2013) Whole genome, whole population sequencing reveals that loss of signaling networks is the major adaptive strategy in a constant environment. PLoS Genet 9:e1003972. https://doi.org/10.1371/journal.pgen.1003972
Liu Q, Liu H, Yang Y, Zhang X, Bai Y, Qiao M, Xu H (2014) Scarless gene deletion using mazF as a new counter-selection marker and an improved deletion cassette assembly method in Saccharomyces cerevisiae. J Gen Appl Microbiol 60:89–93. https://doi.org/10.2323/jgam.60.89
Merico A, Ragni E, Galafassi S, Popolo L, Compagno C (2011) Generation of an evolved Saccharomyces cerevisiae strain with a high freeze tolerance and an improved ability to grow on glycerol. J Ind Microbiol Biotechnol 38:1037–1044. https://doi.org/10.1007/s10295-010-0878-3
Ookubo A, Hirasawa T, Yoshikawa K, Nagahisa K, Furusawa C, Shimizu H (2008) Improvement of L-lactate production by CYB2 gene disruption in a recombinant Saccharomyces cerevisiae strain under low pH condition. Biosci Biotechnol Biochem 72:3063–3066. https://doi.org/10.1271/bbb.80493
Parawira W (2009) Biotechnological production of biodiesel fuel using biocatalysed transesterification: a review. Crit Rev Biotechnol 29:82–93. https://doi.org/10.1080/07388550902823674
Posas F, Saito H (1997) Osmotic activation of the HOG MAPK pathway via Ste11p MAPKKK: scaffold role of Pbs2p MAPKK. Science 276:1702–1705. https://doi.org/10.1126/science.276.5319.1702
Posas F, Wurgler-Murphy SM, Maeda T, Witten EA, Thai TC, Saito H (1996) Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 “two-component” osmosensor. Cell 86:865–875. https://doi.org/10.1016/s0092-8674(00)80162-2
Rep M, Krantz M, Thevelein JM, Hohmann S (2000) The transcriptional response of Saccharomyces cerevisiae to osmotic shock. Hot1p and Msn2p/Msn4p are required for the induction of subsets of high osmolarity glycerol pathway-dependent genes. J Biol Chem 275:8290–8300. https://doi.org/10.1074/jbc.275.12.8290
Strucko T, Zirngibl K, Pereira F, Kafkia E, Mohamed ET, Rettel M, Stein F, Feist AM, Jouhten P, Patil KR, Forster J (2018) Laboratory evolution reveals regulatory and metabolic trade-offs of glycerol utilization in Saccharomyces cerevisiae. Metab Eng 47:73–82. https://doi.org/10.1016/j.ymben.2018.03.006
Swinnen S, Klein M, Carrillo M, McInnes J, Nguyen HTT, Nevoigt E (2013) Re-evaluation of glycerol utilization in Saccharomyces cerevisiae: characterization of an isolate that grows on glycerol without supporting supplements. Biotechnol Biofuels 6:157. https://doi.org/10.1186/1754-6834-6-157
Tokuyama K, Ohno S, Yoshikawa K, Hirasawa T, Tanaka S, Furusawa C, Shimizu H (2014) Increased 3-hydroxypropionic acid production from glycerol, by modification of central metabolism in Escherichia coli. Microb Cell Fact 13:64. https://doi.org/10.1186/1475-2859-13-64
Vikromvarasiri N, Shirai T, Kondo A (2021) Metabolic engineering design to enhance (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis based on flux balance analysis. Microb Cell Fact 20:196. https://doi.org/10.1186/s12934-021-01688-y
Yuzawa T, Shirai T, Orishimo R, Kawai K, Kondo A, Hirasawa T (2021) 13C-metabolic flux analysis in glycerol-assimilating strains of Saccharomyces cerevisiae. J Gen Appl Microbiol 67:142–149. https://doi.org/10.2323/jgam.2020.10.001
Zhu MM, Lawman PD, Cameron DC (2002) Improving 1,3-propanediol production from glycerol in a metabolically engineered Escherichia coli by reducing accumulation of sn-glycerol-3-phosphate. Biotechnol Prog 18:694–699. https://doi.org/10.1021/bp020281+
Acknowledgements
The authors thank Hazuki Kotani (RIKEN) and Tomoya Maeda (Hokkaido University and RIKEN) for genome resequencing analysis and the Biomaterials Analysis Division, Open Facility Center (Tokyo Institute of Technology) for technical assistance with Sanger DNA sequencing analysis.
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This work was supported in part by JSPS KAKENHI Grant Number JP16K14881 to TH.
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Conceptualization: TH; Methodology: MS, KN, ST, TH; Formal analysis and investigation: MS, KN, ST, CF, TH; Writing - original draft preparation: TH; Writing - review and editing: MS, KN, CF.
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Sone, M., Navanopparatsakul, K., Takahashi, S. et al. Loss of function of Hog1 improves glycerol assimilation in Saccharomyces cerevisiae. World J Microbiol Biotechnol 39, 255 (2023). https://doi.org/10.1007/s11274-023-03696-z
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DOI: https://doi.org/10.1007/s11274-023-03696-z