Abstract
Scatter-hoarding animals are crucial in seed dispersal of nut-bearing plants. We used the holm oak Quercus ilex—wood mouse Apodemus sylvaticus mutualism as a model system to evaluate the relative importance of seed size and fat content on scatter-hoarders’ foraging decisions influencing oak dispersal and potential recruitment. We performed a field experiment in which we offered holm oak acorns with contrasting seed size (2 vs 5 g) and fat content (3 vs 11%). Moreover, to test if the strength of these seed trait effects was context-dependent, experimental acorns were placed in small fragments, where natural regeneration is scarce or absent, and forest habitats. In small fragments, rodents had to face increased intraspecific competition for acorns and reduced anti-predator cover during transportation. As a result, they became more selective to ensure rapid acquisition of most valuable food items but, in turn, transported seeds closer to avoid unaffordable predation risks. During harvesting and caching, larger acorns were prioritized and preferentially cached. Fat content only had a minor effect in harvesting preferences. In contrast, in forest sites, where rodent abundance was four times lower and understory cover was well-developed, rodents were not selective but provided enhanced dispersal services to oaks (caching rates were 75% higher). From the plants’ perspective, our results imply that the benefits of producing costly seeds are context-dependent. Seed traits modified harvesting and caching rates only when rodents were forced to forage more efficiently in response to increased intraspecific competition. However, when landscape traits limited cache protection strategies, a more selective foraging behavior by scatter-hoarders did not result in enhanced dispersal services. Overall, our result shows that successful dispersal of acorns depends on how specific traits modulate their value and how landscape properties affect rodents’ ability to safeguard them for later consumption.
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Introduction
Scatter-hoarding animals are crucial in seed dispersal of nut-bearing plants (Vander Wall 1990). From the animals’ view, storing seeds in individual caches allows them to equalize food availability over space, and most importantly, over time ensuring food supply during periods of scarcity. From the plants’ perspective, transportation and burial reduce seed predation close to source trees and maintain seed viability over longer periods of time (Vander Wall 2001; Gómez et al. 2008).
Among scatter-hoarders, rodents often dominate local dynamics of forest regeneration (Jansen et al. 2004; Gómez et al. 2008; Pesendorfer et al. 2016) and may become the only seed dispersers in anthropogenic landscapes (Morán-López et al. 2015). Thus, a better understanding of the behavioral mechanisms driving rodents’ foraging decisions will provide valuable information about seed dispersal and regeneration patterns of nut-bearing plants. Scatter-hoarding can be viewed as a step-wise process that begins when an animal encounters a seed and ends when the animal decides where and when to consume it. It can be divided into four consecutive stages where the hoarder decides whether (1) to ignore or manipulate an encountered seed; (2) to carry it away or consume it in situ; (3) upon removal, how far to carry it; and (4) to eat or cache the transported seed (Wang et al. 2013). During this process, animals balance the costs and benefits of each foraging decision according to their internal motivations, seed traits, and environmental factors (Lichti et al. 2017).
Regarding seed traits, size and nutrient rewards affect hoarders’ foraging decisions. In general, larger seeds are harvested more rapidly, transported further and preferentially stored (e.g. Gómez et al. 2008; Pérez-Ramos et al. 2008; Wang and Chen 2008, 2009; Perea et al. 2012; Sunyer et al. 2014). All else being equal, larger seeds contain more energy and nutrients (Jansen et al. 2004). Thus, by preferentially harvesting and caching them, rodents rapidly sequester the most valuable food items whereas by transporting them further they decrease cache pilferage risks (Lichti et al. 2017). Among nutrients, fat is tightly linked to energy-intake optimization strategies, and it is a fundamental component of mammals’ diet (Wang and Chen 2012). Like larger seeds, fat-rich seeds are usually removed faster (Xiao et al. 2006b; Wang et al. 2013; Wang and Yang 2014). However, fat content effects on dispersal patterns (distance and caching rates) are less clear (Wang and Yang 2014; Lichti et al. 2017). Therefore, maintaining other traits constant, larger and fat-rich seeds are expected to be preferentially removed and stored, and hence, to show higher probabilities of dispersal.
During post-dispersal stages, larger acorns show higher germination rates and produce seedlings more resistant to stressful environmental conditions (Tecklin and McCreary 1991; Gómez 2004; Sage et al. 2011). Thus, on the one hand, producing large seeds may promote seedling recruitment by increasing both the quantity and the quality component of seed dispersal effectiveness (Schupp et al. 2010). On the other hand, larger acorns also entail greater costs per embryo, which may reduce acorn crop (Alejano et al. 2011; Martin et al. 2015). However, the value of food items is context-dependent. In many cases, rodents have to balance between maximizing foraging efficiency and minimizing risks (Lima et al. 1985). For instance, in risky environments like open microhabitats or under bright moonlight (Díaz 1992; Prugh and Golde 2014), rodents tend to minimize seed handling which reduces their discrimination ability, and hence, the influence of seed traits on their harvesting preferences is weaker (Perea et al. 2011; Sunyer et al. 2013). In contrast, when intraspecific competition for seeds is high, rodents invest more effort in safeguarding the most valuable food items from pilferers (Moore et al. 2007; Gálvez et al. 2009), which results in stronger seed trait effects on choice and storage (Pons and Pausas 2007). Thus, whether or not producing more attractive acorns results in enhanced dispersal services is expected to strongly depend on external factors modulating rodents’ foraging decisions.
A better understanding of how environmental conditions affect scatter-hoarders’ foraging decisions is particularly relevant for oaks. A high proportion of oak woodlands are subjected to anthropogenic disturbances (Santos and Tellería 1998; Dey 2014) that impact risk perception by rodents, per capita acorn availability (Morán-López et al. 2016a, b), as well as seedling recruitment (review in Pulido and Díaz 2005). Therefore, to have a full picture of seed trait effects on successful dispersal, the environmental context in which acorn–rodent encounters occur should be acknowledged. In this work, we use the conditional mutualism among holm oak (Quercus ilex) and the wood mouse (Apodemus sylvaticus) as a model system to test the relative importance of seed size and fat content effects on rodents’ scatter-hoarding decisions. Holm oak acorns provided us a unique opportunity to test size and fat content effects on mouse preferences using natural seeds instead of artificial ones. There are two subspecies that differ in their fat content but hold similar levels of protein and tannins (Gea-Izquierdo et al. 2006). Additionally, to evaluate how environmental conditions modify seed trait effects, we performed experiments in habitats with contrasting levels of anti-predator cover and rodent abundance—forest sites and small fragments (Morán-López et al. 2015). Labeled acorns of different size (2 vs 5 g) and fat content (3% vs 11%) were offered in forest and small fragments and left for depletion by rodents. With the help of video-recordings, we evaluated acorn trait effects on harvesting preferences, transportation distances, and caching rates.
We expected that seed size would promote dispersal across all stages of the scatter-hoarding process while fat content would mainly affect harvesting time (Wang and Chen 2009; Wang et al. 2013). We also hypothesized that mice would show hierarchical foraging preferences prioritizing seed size over nutrient content (Wang and Chen 2009). Finally, we expected that foraging behaviors aimed at ensuring rapid acquisition of the most valuable food resources (e.g. quick harvesting) would be more important in forest fragments, due to increased competition for acorns (Moore et al. 2007; Sunyer et al. 2013). In contrast, low anti-predator cover in these areas would constrain mouse movements and cache protection strategies resulting in impoverished dispersal services (Morán-López et al. 2015, 2016b).
Materials and methods
Study site and experimental design
Fieldwork was carried out in the northern plateau of the Iberian Peninsula close to the locality of Lerma (42º5′N,3º45′W, 930 m a.s.l.). Landscape is composed of an archipelago holm oak forests located in an extensive treeless agricultural region (Santos and Tellería 1998). The dominant tree is holm oak, with isolated Lusitanian oaks Q. faginea and Spanish junipers Juniperus thurifera and understory shrubs typical from wet and cool Supramediterranean localities (e.g. Cistus laurifolius, Genista scorpius, Thymus zygis). Annual precipitation is 567 mm and annual mean temperature 11 °C.
To evaluate the behavioral plasticity of rodents, we selected three plots located in forest sites (> 100 ha) more than 2 km apart and six small forest fragments (0.03 ± 0.01 ha) (Table 4). For each fragmentation category (forest and small fragments), we selected 12 focal trees. Each tree was supplied with a full-factorial design of seeds with contrasting sizes (big and small, 5.00 ± 0.07, 2.37 ± 0.05 g) and fat content (poor and rich, 3 acorns per size–fat content combination, N = 12 acorns per tree). Acorns of subsp. ballota were considered fat-rich while acorns from the subsp. ilex were considered fat poor according to Gea-Izquierdo (2006). To ensure that such classification was correct, we subsequently analyzed the organoleptic composition of acorns from the same source trees as offered ones (see below). Trees located at small fragments show much higher crops than that from forest sites (Morán-López et al. 2016a, b). Overall acorn availability (Moore et al. 2007; Sunyer et al. 2014) and the contrast of traits between environmental acorns and those offered (Lichti et al. 2014) can modify rodents’ foraging decisions. Therefore, to test for net size and fat content effects, the experiment was carried in March 2013, outside the acorn fall season (November–January) and when more than 5% of cached acorns have already been recovered (Gómez et al. 2008; Perea et al. 2011b, Morán-López pers. obs.). The timing of the experiment is unlikely to have biased rodents’ foraging behavior since previous studies in the area have found similar responses to labeled acorns (e.g., similar overall removal rates) during and outside the acorn fall season (Morán-López et al. 2015).
Experimental acorns were individually marked with a metal wire (Ø 0.6 mm) with a numbered plastic tag attached to it (Xiao et al. 2006a). All acorns were manipulated with gloves to avoid effect of human scent (Duncan et al. 2002). They were placed beneath canopies of focal trees and were protected with 35 × 35 × 15 cm wire cages with 6 cm mesh that only allowed the entrance of rodents. To monitor mouse activity in each focal tree, we installed a video-recording device that consisted on an OmniVision CMOS 380 LTV (3.6 mm lens) camera focused on cages. The system was powered with car batteries and was fully autonomous for continuous recording during 3 consecutive days (Gallego et al. 2017). Supply points were checked one night after acorn offering.
Fragmentation effects on rodent abundance and anti-predator cover
Rodents were lived-trapped within 3 days of new moon in March 2013 (to control for moonlight effects on reduced rodent activity; Díaz 1992; Perea et al. 2011) by means of Sherman live traps. Forest sites were sampled by means of grids of 6 × 5 traps spaced 10 m, covering an area of 0.3 ha. Small fragments were sampled by means of 1–6 pairs of traps (depending on fragment size, measured on 1:5000 aerial photographs) distributed over the entire fragment. Traps were operated following the standard guidelines of baiting and comfort (Díaz et al. 2010). Trapped mice were identified to species, sexed, and marked (see Morán-López et al. 2015 for details). Complete trapping was corroborated by plotting the number of new recaptures per night against the number of days of exposure. To take into account potential biases in seed trait effects due to rodents’ gender (Rosalino et al. 2013), sex ratios of forest fragments were calculated.
Habitat structure was estimated over two 20 m-long, 0.5 m-wide transects established in random directions from each focal tree. Along these transects, we estimated the proportion of open land (bare ground and herbs) and anti-predator cover (shrubs and tree canopies with resprout). Transect length was determined by transportation distances during primary dispersal (< 20 m in most cases) (Gómez et al. 2008, Puerta-Piñero et al. 2010, Morán-López et al. 2015). This way, our estimations of habitat structure were used as a proxy of anti-predator cover availability during acorn transportation from the source point.
Nutritional traits of experimental acorns
In November, we collected for chemical analyses sound and uninfested acorns from 37 to 46 mother trees, Q. ilex subsp. ballota and subsp. ilex, respectively. In each source tree, we collected between 45 and 60 acorns that were subsequently separated in three groups of 15–20 acorns (Nilex = 1887; Nballota = 1930). Firstly, to ensure that rodents’ selectivity was due to fat content and not to differences in acorn morphology (Muñoz et al. 2012), length and width was measured with digital calipers to the nearest millimeter and a shape parameter (length:width ratio) was calculated. Then, hulls were removed and kernels of acorns from the same group were milled. This flour was dried in a forced-air drier during 48 h at 45 °C. It was then sieved (1-mm mesh) to obtain a fine homogeneous flour. Samples were vacuum-sealed and stored at 4 °C until laboratory analyses (Fernández et al. 2004).
Near-infrared spectroscopy (NIR) was used to translate reflectance spectra of acorns into chemical composition. To take into account potential co-variation among acorn traits, for each source tree we estimated the percentage of crude fat, crude protein, and polyphenols per dry weight. We used polyphenolic compounds as a surrogate of tannins since they constitute the most common polyphenol in acorns (Luczaj et al. 2014). Samples were analyzed at the University of Cordoba NIRS Service (http://www.uco.es/servicios/scai/nir.html). They were scanned using a Foss-NIRSystems 6500 System II spectrophotometer (Foss-NIRSystems Inc., Silver Spring, MD, USA). Reflectance spectra were collected every 2 nm, from 400 to 2500 nm. Data were stored using WinISI II software (Infrasoft International Port Matilda, PA, USA). For translating reflectance values into organoleptic properties, we used the NIR equation developed by Galvan et al. (2012). Samples whose chemical composition was very different from samples used to develop NIR equations (GH > 3) were analyzed in Serida (http://www.serida.org) and “Agroalimentario de Córdoba” laboratories.
Mouse foraging activity
First, we estimated the amount of time mice took to deplete offered acorns upon the first encounter (depletion time hereafter). Then, we evaluated mouse activity during foraging events, which were defined from the entry of an individual into the cage up to its exit. For each foraging event, we measured the duration—to the nearest second—and the proportion of time spent searching for food (moving head down), handling acorns, or vigilant (standing head up) (Hochman and Kotler 2007; Gallego et al. 2017). To evaluate mouse foraging decisions, we divided the scatter-hoarding process into three consecutive events: (1) acorn choice, (2) transportation, and (3) storage. In each foraging event, we noted which acorn was harvested and whether it was transported outside the cage or not. Transported acorns were searched within a radius of 30 m the day after exposure, and when not found, during the following week. We noted transported distances and whether the handled acorns were cached or not.
Data analysis
Acorn traits and environmental variables
To assess if experimental acorns differed in their chemical content, we used generalized linear models with a binomial response (logit link). Our response variables were proportion of fat, protein, and tannin content (averaged per tree). Our fixed effect was subsp. (ilex vs ballota). To evaluate if mouse abundance and sex ratios differed between forest sites and small fragments, we used generalized linear models. We modeled the number of mice per 100 traps per night with a Poisson distribution (log link) and the proportion of females with a binomial distribution (logit link). Finally, to test if fragmentation levels showed different anti-predator cover, we used hierarchical binomial models (logit link). Our response variable was the proportion of open land cover (averaged per tree) and our fixed-effect fragmentation level (forest vs small fragment). We introduced site as random factor to take into account spatial autocorrelation of trees located within the same forest sites or within groups of nearby fragments (areas of 35 ha). We fitted all these models using maximum likelihood approximation (lme4 package version 1.0–5., Bates et al. 2011).
Mouse activity
Depletion time was modeled as a Poisson process (log link), which depended on forest fragmentation. Regarding individual events, total event duration (in seconds) was modeled as a Poisson (log link) process, and proportion of time invested on acorn handling and searching were modeled as binomial process (logit link). In all cases, mouse response depended on the fragmentation level (forest, small fragment) and the number of acorns available at the time of the event. In the case of vigilant behaviors, low number of events forced us to use zero-inflated binomial models. The probability of having a vigilant behavior depended on forest fragmentation, and once this occurred time investment depended on the number of acorns available. In all cases, tree nested in site was introduced as a random factor. See Appendix 1 for model specification.
Mouse scatter-hoarding decisions
To assess acorn choice by rodents, we fitted a hierarchical multinomial model that took into account the number and types (fat content and size) of seeds available in each foraging event. Reference seed trait combination was set to small and fat-rich acorns. Moreover, as rodents may change their selectivity with acorn depletion (Mitchell 1990), we also analyzed the probability of removal during the first or last three foraging events. For this purpose, we used two hierarchical binomial models (logit link), in which our response variables were (1) the first three and (2) the last three acorns removed. In all cases, the probability of removal was modulated by fat content (rich vs poor) and size (small vs big). The strength of these effects depended on fragmentation, which is equivalent to introducing double interactions between fragmentation and acorn traits. Tree nested in site was introduced as a random factor.
To evaluate acorn transportation distances, we used a hierarchical Gaussian model with log-transformed data. The same structure of fixed and random effects as in acorn choice was used. Additionally, we evaluated habitat-type effects on acorn transportation kernels. For this purpose, we estimated skewness, kurtosis, and quantiles of both habitat types using fitdistributionplus package in R (Delignette-Muller et al. 2010). Finally, we analyzed the probability of acorn caching (yes or no) by means of a hierarchical binomial model (logit link). Again, fat content and size modified the probability of storage. The strength of these effects was modulated by fragmentation, and tree nested in site was introduced as a random factor. We fitted models of mouse activity and foraging decisions employing a Bayesian approach with JAGS 3.4.0 (Plumer 2003). For model structure and parameter prior distributions, see Appendix 1. We checked for convergence (Rhat < 1.03) for all model parameters and calculated the mean value of posterior distributions as point estimates and the 95% Highest Posterior Density interval (HPD), also called credible interval, as a measure of uncertainty around point estimates (Gelman and Hill 2007). In addition, we calculated the proportion of the posterior distribution with the same sign as the mean (f). The effective sample size for each Monte Carlo Markov Chain (MCMC) was always greater than 900. To assess the predictive power of our models, we performed posterior predictive checks (in Appendix 3, Gelman and Hill 2007).
Results
Acorn traits
As expected, main differences in acorn chemical composition were found in fat content. Subsp. ballota acorns showed almost four times higher fat content than subsp. ilex acorns (10.81 vs 2.80%, respectively; model estimate 1.43 ± 0.11, P < 0.01). Acorns from the two subspecies did not differ in protein or tannin content (P = 0.09 and P = 0.33, respectively, Table 1). Offered acorns had the same shape irrespectively of the population of origin (2.12 ± 0.07, 2.24 ± 0.05, length:width ratio; subsp. ilex and subsp. ballota, respectively).
Fragmentation effects on rodent abundance and anti-predator cover
All captured rodents were Apodemus sylvaticus. Forest fragmentation significantly affected mouse abundance (estimate 1.47 ± 0.36, P < 0.01), which was almost four times higher in small fragments than in forest sites (13.00 ± 2.49 vs 3.33 ± 0.64 mice/100 traps per night). However, sex ratios did not differ between habitat types (estimate 0.74 ± 0.83, P = 0.37). Regarding anti-predator cover, the proportion of open land around focal trees was greater in small forest fragments (0.62 ± 0.18) than in forest sites (0.28 ± 0.14) (estimate 1.39 ± 0.20, P < 0.01).
Mouse activity
Mice harvested 100% of offered seeds during the first night of the experiment. Three cameras failed during the experiment leading to a final sample size of 21 focal trees, 11 of them located in forest sites and 10 of them in forest fragments. We obtained 313 video-recordings out of which 252 corresponded to foraging events. Duration of foraging bouts was similar in both habitats (11.57 ± 0.20 s) and remained unchanged with acorn depletion (Table 2, total time). In general, patterns of time investment in different activities were consistent between habitats. Rodents spent most time in acorn searching and handling while vigilance was a secondary activity (Fig. 1). The amount of time invested in searching and handling did not differ between habitat types though the probability of having a vigilant behavior tended to be higher in forest fragments.
Fragmentation modulated the capacity of rodents to adapt their behavior to acorn availability (Table 2, number of acorn effects). Although average handling times did not differ between habitats (0.44 ± 0.05 and 0.50 ± 0.04 s for forests and fragments, respectively, Table 2); in forest fragments, mice tended to spend more time in acorn handling when all acorns were available. In fact, during the first three foraging bouts, mice spent 29.3% more time in handling activities than in forests. In contrast, in forest sites mice did not adapt their foraging behavior to acorn availability. It only modified time devoted to vigilant behaviors, which represented less than 1% of total time (Table 2).
Mouse scatter-hoarding decisions
The amount of time taken to deplete offered acorns was similar in both types of habitats (48.38 ± 1.52 min on average, mean effect 0.05, f = 0.50). Nonetheless, mice foraging decisions differed between habitats. When taking into account all foraging bouts, acorn choice was driven by size in small fragments (mean effect 1.95, f = 0.99; multinomial model) while in forest sites size effects were small (mean effect 0.51, f = 0.88). In general, fat content did not affect the overall probability of acorn removal. However, in small forest fragments, within the same seed size category mice tended to preferentially remove fat-rich acorns leading to a late harvesting of small fat-poor ones (Table 3, Fig. 2b).
Maximum and mean transportation distances were similar in both habitat types (22.08 vs 21.53 m; 3.09 vs 4.06 m; forest and fragment sites, respectively). Nonetheless, acorn transportation kernels showed different shapes, with forests showing fatter tails (estimated skewness: 1.61, 2.49, and kurtosis: 4.95, 9.45; forest and fragments, respectively). As a result, the probability of transportation beyond the canopy of the source tree (> 2 m) was higher in forests (third quantile: 6.47 vs 2.98 m; Fig. 3a). At first glance, acorn traits did not modify transportation distances at forest sites while in small fragments fat-poor acorns were transported further (Fig. 3a, 5.47 ± 0.82 vs 1.91 ± 0.31 m, fat-poor vs fat-rich). However, there was a high variability on fat content effects among source trees leading to a non-significant global effect (Table 3). Regarding caching rates, they were higher in forest sites than in small fragments (23.07% vs 13.17% of handled seeds on average). Acorn traits in forest sites did not affect caching rates whereas in small fragments larger acorns had a higher probability of being cached (23% vs 3%, respectively; Table 3, Fig. 3b).
Discussion
Seed encounter by animals represents the first stage of the scatter-hoarding process. During this step, time devoted to different activities will be tightly linked to the effect of acorn traits on the probability of manipulation. In small fragments, where intraspecific competition for seeds was much stronger, rodents adapted their behavior to food availability. When all acorn types were available, they spent more time in acorn handling. This may have helped rodents at gathering information about acorn traits, and hence, at foraging more efficiently by discriminating more valuable food items (Perea et al. 2011; Sunyer et al. 2013). In forest habitats, where competition for seeds was much lower, this adaptive behavior disappeared. On one hand, these findings support the idea that in areas with high competition for resources foragers go from a selective behavior to an opportunistic one as food availability decreases, whereas in areas with fewer competitors they mostly behave as generalists (Mitchell 1990, Perea et al. 2011). On the other hand, they suggest that high intraspecific competition for seeds promotes a more selective harvesting by rodents.
Accordingly, acorn trait effects on mouse scatter-hoarding decisions were stronger in small forest fragments. As found in previous studies, larger acorns were harvested faster (e.g. Perea et al. 2012; Sunyer et al. 2014) and preferentially cached (e.g. Gómez, et al. 2008; Wang and Chen 2008). Fat content played a secondary role, only enhancing the positive effects of size on seed choice. Thus, our results show that seed size rather than fat content affects the probability of dispersal. This may be because by prioritizing seed size, mice optimize the number of caches to be tracked for the same amount of stored food, since they use size as a proxy of other seed traits (Wang and Chen 2009; Wang et al. 2013; Wang and Yang 2014), or simply because fat content is more cryptic. From the plants’ perspective, enhanced dispersal services of larger seeds may imply a double benefit. Larger acorns tend to produce seedlings with higher probabilities to overcome the annual summer drought (Gómez 2004), which is one of the main causes of seedling death in Mediterranean areas (Smit et al. 2008).
Enhanced rodent abundance in fragmented areas has been linked to impoverished dispersal services (Morán-López et al. 2015) and even to regeneration failure due to complete predation of acorn crops (Santos and Telleria 1997). However, preferential caching of “high quality” seeds due to increased intraspecific competition may attenuate such negative impacts, provided that rodents act as moderately efficient acorn dispersers (sensu Gómez et al. 2008). If this was the case, our results suggest that the role of rodents as catalyst of forest regeneration after crop abandonment (Morán-López et al. 2016a, b) could be greater than previously acknowledged. Unfortunately, mowing of surrounding fields precluded us from evaluating seedling emergence and survival. Future studies in fragmented areas surrounded by abandoned croplands will provide valuable information in this regard.
In contrast to small fragments, in forest sites acorn trait effects were weaker or null. They did not affect the overall probability of removal and only size was negatively linked to late harvesting. Most importantly, seed traits did not affect caching rates. Low intraspecific competition for seeds may have discouraged rodents to forage efficiently (Sunyer et al. 2013). These results support the idea that food value, and hence, the relative importance of seed trait effects on successful dispersal are context-dependent (Lichti et al. 2017). Certain environmental conditions should be met so that rodents forage selectively, and as a result, specific traits affect seed fate. Otherwise, producing expensive seeds may make no difference on the probability of dispersal, an essential initial step for recruitment.
It is important to note, however, that even though rodents were less responsive to acorn traits in forest sites, they provided enhanced dispersal services to oaks. For instance, caching rates were 75% higher. In small fragments, rodents behaved selectively in all stages of the scatter-hoarding process except for transportation. This suggests that environmental conditions in these areas constrain the ability of rodents to carry and cache the most valuable acorns far from tree canopies, where pilferage risks are high. Most probably, lack of anti-predator cover triggered risks during transportation leading to higher acorn predation rates (see Morán-López et al. 2015 for similar results in the study area). Overall, our results point out that when environmental conditions constrain cache protection strategies, a more selective foraging behavior may not necessarily imply enhanced dispersal services. Besides, they support the idea that high proportion of open land cover is the main environmental factor limiting acorn dispersal in fragmented areas (Morán-López et al. 2015, 2016b).
Conclusions
In general, our results confirm that seed size is a major driver of scatter-hoarders’ foraging decisions while fat content plays a secondary role. Moreover, they show that seed trait effects on successful dispersal are context-dependent. Only in forest fragments, where rodents were forced to forage more efficiently due to increased intraspecific competition for seeds, size promoted rapid harvesting and enhanced caching rates. Although this behavior may mitigate the negative impacts of fragmentation on oak regeneration, caching rates were much lower than in forest sites. This suggests that when landscape traits (e.g., high proportions of open land) limit scatter-hoarders’ caching strategies, a more selective foraging behavior does not warrant enhanced seed dispersal services for oaks.
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Acknowledgements
We acknowledge Miguel Ángel Fernández, Xavier Rotllàn Puig, David López Quiroga and Dulce Flores Rentería for their invaluable technical support during fieldwork. Morán-López T. was beneficiary of a FPI grant (funded by the Spanish Government (BES-2011-048346) and a CONICET post-doctoral fellowship (funded by the Argentinian Government). This paper is a contribution to the Spanish-funded projects VULGLO (CGL2010–22180-C03–03), VERONICA (CGL2013-42271-P) and REMEDINAL 2 & 3 (CM S2009 AMB 1783; S2013/MAE-2719). NIR data were obtained using NIRS hardware and software provided by the NIR/MIR Spectroscopy Unit of the SCAI at the University of Cordoba, Campus de Excelencia CEIA3 (Spain).
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Appendices
Appendix 1: Model structure and priors for every response variable
Total foraging time
We modeled the total foraging time invested by mice as a Poisson process that depended on the fragmentation level (forest or fragment) and the number of acorns available. As acorns were in trees located within sites, we introduced tree nested in site as random factors.
Prior distributions—parameters at tree level
Hyper-prior distributions—parameters at site level
\(\sigma^{b0} \sim {\text{uniform}}\quad (0, 100)\)
\(y _{i,j,k}\) is the total foraging time of event i at tree j in site k. b0 is the mean time invested in non-fragmented sites, b1 depicts fragmentation effects. b2 represents the effect of the number of acorns available, which depends on forest fragmentation (α and δ). Each focal tree had a mean b0 and b2 effect (\(b0_{j,k} , b2_{j,k} )\), centered on the site where the tree is located \(\mu_{k}^{b0} ,\,\mu_{k}^{b2}\).
Handling—searching time
We modeled proportion of time invested in handling or searching as a binomial process that depended on the fragmentation level (forest or fragment) and the number of acorns available. As acorns were in trees located within sites, we introduced tree nested in site as random factors.
Prior distributions—parameters at tree level
Hyper-prior distributions—parameters at site level
\(\sigma^{b0} \sim {\text{uniform}} \quad(0, 100)\)
where \(y _{i,j,k}\) is either the searching or the handling time of event i at tree j in site k. Here, N is the total time of the foraging ith event. b0 is the mean time invested in non-fragmented sites, b1 depicts fragmentation effects. b2 represents the effect of the number of acorns available, which depends on forest fragmentation (α and δ). Each focal tree had a mean b0 and b2 effect \(\left( {b0_{j,k} , b2_{j,k} } \right)\), centered on the site where the tree is located \(\mu_{k}^{b0} ,\,\mu_{k}^{b2}\).
Vigilance time
We modeled time invested in vigilance as a zero-inflated binomial process that depended on the fragmentation level (forest or forest fragment) and the number of acorns available. As acorns were in trees located within sites, we introduced tree nested in site as random factors.
Prior distributions—parameters at tree level
Hyper-prior distributions—parameters at site level
\(y _{i,j,k}\) is whether the mouse invested time in vigilance or not during event i at tree j in site k, and \(r _{i,j,k}\) is the time invested in vigilance during event i at tree j in site k. The probability of investing time in vigilance (p1) depends on fragmentation level (forest or forest fragment). a0 is the mean time invested in non-fragmented sites and a1 depicts fragmentation effects. The proportion of time invested in vigilance (p2) depends on the number of acorns available. b2 represents the effect of the number of acorns available, which depends on forest fragmentation (α and δ). Each focal tree had a mean b0 and b2 effect \(\left( {a0_{j,k} , b1_{j,k} } \right)\), centered on the site where the tree is located \(\mu_{k}^{bo} ,\,\mu_{k}^{b1}\).
Depletion time
We modeled the depletion time (in seconds) as a Poisson process that depended on the fragmentation level (forest or fragment). As acorns were in trees located within sites, we introduced tree nested in site as random factors.
Prior distributions
Hyper-prior distributions—parameters at site level
\(y _{j,k}\) is the depletion time at tree j in site k. α is the mean time invested in non-fragmented sites and β depicts fragmentation effects. Each site had a mean α k .
Multiple choice model
We modeled the probability of an acorn being removed taking into account the number and types (fat content and size) of acorns available in each foraging event. This probability depended on acorns fat content and size. As acorns were in trees located within sites, we introduced tree nested in site as random factors.
Prior distributions—parameters at tree level
Hyper-prior distributions—parameters at site level
\(y _{i,j,k}\) is a binary variable that represents whether the acorn i at tree j in site k was removed (1), or not (0). N represents the number of acorns present in that foraging event at that tree. b1 is the probability of being removed given that the acorn is small and has low fat content, b2 is the effect of fat content, and b3 the effect of size. These effects depended on forest fragmentation (α and δ). Each focal tree had a mean b1, b2, and b3 effect (\(b1_{j,k} , b2_{j,k} ,b3_{j,k} )\), centered on the site where the tree was located \(\left(mu_{k}^{b1} ,\,\mu_{k}^{b2} ,\,\mu_{k}^{b3}\right)\).
First and last acorns removed
We modeled the probability of an acorn being removed first or last as a Bernoulli process that depended on their fat content and size. As acorns were in trees located within sites, we introduced tree nested in site as random factors.
Prior distributions—parameters at tree level
Hyper-prior distributions—parameters at site level
\(y _{i,j,k}\) is a binary variable that represents whether the acorn i at tree j in site k was removed first/last (1), or not (0). b1 is the probability of being removed first or last given that the acorn is small and has low fat content, b2 the effect of fat content, and b3 the effect of size. These effects depended on forest fragmentation (α and δ). Each focal tree had a mean b1, b2, and b3 effect (\(b1_{j,k} , b2_{j,k} ,b3_{j,k} )\), centered on the site where the tree was located \(\mu_{k}^{b1} ,\;\mu_{k}^{b2} ,\;\mu_{k}^{b3}\).
Dispersal distance
We modeled how far the mice transported the acorns with a normal distribution. We used a logarithmic transformation in other to meet normality. Dispersal distances depended on acorns fat content and size. As acorns were in trees located within sites, we introduced tree nested in site as random factors.
Prior distributions—parameters at tree level
Hyper-prior distributions—parameters at site level
\(y _{i,j,k}\) is the distance acorn i at tree j in site k was dispersed. b1 is the mean distance mice transport seeds given they are small and have low fat content, b2 the effect of fat content, and b3 the effect of size. These effects depended on forest fragmentation (α and δ). Each focal tree had a mean b1, b2, and b3 effect (\(b1_{j,k} , b2_{j,k} ,b3_{j,k} )\), centered on the site where the tree was located \(\mu_{k}^{b1} ,\;\mu_{k}^{b2} ,\;\mu_{k}^{b3}\).
Cached
We modeled if mice consumed or stored the acorns as a Bernoulli process that depended on acorn fat content and size. As acorns were in trees located within sites, we introduced tree nested in site as random factors.
Prior distributions—parameters at tree level
Hyper-prior distributions—parameters at site level
\(y _{i,j,k}\) is a binary variable that represents whether the acorn i at tree j in site k was cached (1), or not (0). b1 is the probability of being cached given that the acorn is small and has low fat content, b2 the effect of fat content and b3 the effect of size. These effects depended on forest fragmentation (α and δ). Each focal tree had a mean b1, b2, and b3 effect (\(b1_{j,k} , b2_{j,k} ,b3_{j,k} )\), centered on the site where the tree was located \(\mu_{k}^{b1} ,\,\mu_{k}^{b2} ,\,\mu_{k}^{b3} \,\).
Appendix 2
See Table 4.
Appendix 3 Posterior predictive checks of Bayesian models
Mouse foraging activity
Mouse foraging decisions
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Morán-López, T., Valladares, F., Tiribelli, F. et al. Fragmentation modifies seed trait effects on scatter-hoarders’ foraging decisions. Plant Ecol 219, 325–342 (2018). https://doi.org/10.1007/s11258-018-0798-2
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DOI: https://doi.org/10.1007/s11258-018-0798-2