Abstract
The immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nano particles (MNPs), preliminarily functionalized with 3-aminopropylsilane and N-(phosphonomethyl) iminodiacetic acid (PMIDA), was studied. Protein binding to MNPs depending on the nature of the surface functional groups, the medium, and the presence of a coupling agent, namely, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), was studied using fluorimetry, UV and IR spectroscopy. It was shown that protein immobilization can occur by both non-covalent and covalent binding with surface groups of MNPs. The best results (up to 150 μg of TagGFP2 per 1 mg of MNPs) were obtained for MNPs preliminarily functionalized with PMIDA and using EDC. The results of this study can be used for the design of imaging agents having both magnetic and optical properties to be applied in biomedicine.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
O. Veiseh, J. Gunn, M. Zhang, Adv. Drug Deliv. Rev., 2010, 62, 284.
C. Xu, S. Sun, Adv. Drug Deliv. Rev., 2013, 65, 732.
S. Laurent, D. Forge, M. Port, A. Roch, C. Robic, L. Vander Elst, R. N. Muller, Chem. Rev., 2008, 108, 2064.
Y.-X. J. Wang, Quant. Imaging Med. Surg., 2011, 1, 35.
J. Gao, H. Gu, Xu, Acc. Chem. Res., 2009, 42, 1097.
A. M. Demin, Yu. V. Kuznetsova, V. P. Krasnov, A. A. Rempel, Dokl. Chem., 2016, 467, 113.
A. M. Demin, M. V. Ulitko, A. S. Minin, Yu. V. Kuznetsova, S. V. Rempel, V. P. Krasnov, A. A. Rempel, Dokl. Chem., 2016, 467, 118.
C.-W. Hung, T. Holomana, P. Kofinas, W. E. Bentley, Biochem. Eng. J., 2008, 38, 164.
I. F. Nata, N. S. El-Safory, C. K. Lee, ACS Appl. Mater. Interfaces, 2011, 3, 3342.
I. S. Lee, N. Lee, J. Park, B. H. Kim, Y.-W. Yi, T. Kim, T. K. Kim, I. H. Lee, S. R. Paik, T. Hyeon, J. Am. Chem. Soc., 2006, 128, 10658.
B. J. Kim, Y. Piao, N. Lee, Y. Park, I.-H. Lee, J.-H. Lee, S. R. Paik, T. Hyeon, Adv. Mater., 2010, 22, 57.
S. Engin, V. Trouillet, C. M. Franz, A. Welle, M. Bruns, D. Wedlich, Langmuir, 2010, 26, 6097.
Z.-F. Gan, J.-S. Jiang, Y. Yang, B. Du, M. Qian, P. Zhang, J. Biomed. Mater. Res. A., 2008, 84A, 10.
O. M. Subach, I. S. Gundorov, M. Yoshimura, F. V. Subach, J. Zhang, D. Gruenwald, E. A. Souslova, D. M. Chudakov, V. V. Verkhusha, Chem. Biol., 2008, 15, 1116.
A. M. Demin, A. G. Pershina, K. V. Nevskaya, L. V. Efimova, N. N. Shchegoleva, M. A. Uimin, D. K. Kuznetsov, V. Ya. Shur, V. P. Krasnov, L. M. Ogorodova, RSC Adv., 2016, 6, 60196.
A. M. Demin, A. G. Pershina, V. V. Ivanov, K. V. Nevskaya, O. B. Shevelev, A. S. Minin, I. V. Byzov, A. E. Sazonov, V. P. Krasnov, L. M. Ogorodov, Inter. J. Nanomed., 2016, 11, 4451.
A. M. Demin, A. V. Mekhaev, A. A. Esin, D. K. Kuznetsov, P. S. Zelenovskiy, V. Y. Shur, V. P. Krasnov, Appl. Surf. Sci., 2018, 440, 1196.
A. M. Demin, A. G. Pershina, A. S. Minin, A. V. Mekhaev, V. V. Ivanov, S. P. Lezhava, A. A. Zakharova, I. V. Byzov, M. A. Uimin, V. P. Krasnov, L. M. Ogorodova, Langmuir, 2018, 34, 3449.
A. M. Demin, A. G. Pershina, V. V. Ivanov, O. B. Shevelev, M. A. Uimin, K. V. Nevskaya, N. N. Shchegoleva, A. S. Minin, A. E. Sazonov, V. P. Krasnov, L. M. Ogorodova, Izv. Vuzov. Fiz. [Bull. of Higher Inst. Phys.], 2015, {dd58}, No. 12/2, 68 (in Russian).
A. M. Demin, O. V. Koryakova, V. P. Krasnov, J. Appl. Spectr., 2014, 81, 565.
H. Fukuda, M. Arai, K. Kuwajima, Biochemistry, 2000, 39, 12025.
T. Maniatis, E. F. Fritsch, J. Sambrook, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York, 1982, 2230 pp.
U. K. Laemmli, Nature, 1970, 227, 680.
M. M. Bradford, Anal. Biochem., 1976, 72, 248.
Author information
Authors and Affiliations
Corresponding author
Additional information
Based on the materials of the IV Interdisciplinary Symposium on Medicinal, Organic, Biological Chemistry and Pharmaceutics (MOBI-ChemPharma 2018) (September 23–26, 2018, Novyi Svet, Crimea).
Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 5, pp. 1088–1095, May, 2019.
Rights and permissions
About this article
Cite this article
Demin, A.M., Valova, M.S., Pershina, A.G. et al. Immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nanoparticles. Russ Chem Bull 68, 1088–1095 (2019). https://doi.org/10.1007/s11172-019-2524-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11172-019-2524-1